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Sci Signal. 2018 Mar 6;11(520). pii: eaah3738. doi: 10.1126/scisignal.aah3738.

cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.

Author information

1
Department of Biology, New York University (NYU), 100 Washington Square East, New York, NY 10003, USA.
2
NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA.
3
Department of Biology, New York University (NYU), 100 Washington Square East, New York, NY 10003, USA. justin.blau@nyu.edu.
4
Center for Genomics and Systems Biology, NYU Abu Dhabi Institute, Abu Dhabi, United Arab Emirates.

Abstract

Genetically encoded fluorescent sensors enable cell-specific measurements of ions and small molecules in real time. Cyclic adenosine monophosphate (cAMP) is one of the most important signaling molecules in virtually all cell types and organisms. We describe cAMPr, a new single-wavelength cAMP sensor. We developed cAMPr in bacteria and embryonic stem cells and validated the sensor in mammalian neurons in vitro and in Drosophila circadian pacemaker neurons in intact brains. Comparison with other single-wavelength cAMP sensors showed that cAMPr improved the quantitative detection of cAMP abundance. In addition, cAMPr is compatible with both single-photon and two-photon imaging. This enabled us to use cAMPr together with the red fluorescent Ca2+ sensor RCaMP1h to simultaneously monitor Ca2+ and cAMP in Drosophila brains. Thus, cAMPr is a new and versatile genetically encoded cAMP sensor.

PMID:
29511120
PMCID:
PMC5863242
[Available on 2018-09-06]
DOI:
10.1126/scisignal.aah3738

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