Format

Send to

Choose Destination
Respir Physiol Neurobiol. 2014 Feb 1;192:134-46. doi: 10.1016/j.resp.2013.12.012. Epub 2013 Dec 26.

Effects of Rho-kinase inhibition in lung tissue with chronic inflammation.

Author information

1
Department of Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil. Electronic address: refragar@gmail.com.
2
Department of Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil. Electronic address: pangelipigati@gmail.com.
3
Department of Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil. Electronic address: szpossa@hotmail.com.
4
Department of Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil. Electronic address: fabiocetinichabrum@yahoo.com.br.
5
Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Ilha do Fundão, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil. Electronic address: deboraxisto@gmail.com.
6
Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Ilha do Fundão, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil. Electronic address: maryantunes@gmail.com.
7
Department of Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil. Electronic address: leick51@yahoo.com.
8
Department of Biological Science, Universidade Federal de São Paulo, 09972-270 Diadema, São Paulo, Brazil. Electronic address: cmaximoprado@gmail.com.
9
Department of Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil. Electronic address: mmartins@usp.br.
10
Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Ilha do Fundão, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil. Electronic address: prmrocco@gmail.com.
11
Department of Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil. Electronic address: iocalvo@uol.com.br.

Abstract

We evaluated whether Rho-kinase inhibition (Y-27632) modulated distal lung responsiveness, inflammation, extracellular matrix remodeling and oxidative stress activation in guinea pigs (GPs) with chronic allergic inflammation. GPs were submitted to inhalation of ovalbumin (OVA-2×/week/4 weeks). From the 5th inhalation on, the Rho-kinase inhibitor group animals were submitted to Y-27632 inhalation 10min before each inhalation of OVA. Seventy-two hours after the seventh inhalation, the oscillatory mechanics of the distal lung strips were assessed under the baseline condition and after the ovalbumin challenge. Subsequently, the lung slices were submitted to morphometry. Rho-kinase inhibition in the ovalbumin-exposed animals attenuated distal lung elastance and resistance, eosinophils, IL-2, IL-4, IL-5, IL-13, TIMP-1, MMP-9, TGF-β, IFN-γ, NF-κB and iNOS-positive cells and the volume fraction of 8-iso-PGF2α, elastic, collagen and actin in alveolar walls compared with the OVA group (P<0.05). Rho-kinase inhibition contributed to the control of distal lung responsiveness, eosinophilic and Th1/Th2 responses and extracellular matrix remodeling in an animal model of chronic allergic inflammation.

KEYWORDS:

Asthma; Distal lung; Inflammation; Rho-kinase; Y-27632

PMID:
24373838
DOI:
10.1016/j.resp.2013.12.012
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center