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Development. 2018 Dec 18;145(24). pii: dev170142. doi: 10.1242/dev.170142.

Spatial and temporal inhibition of FGFR2b ligands reveals continuous requirements and novel targets in mouse inner ear morphogenesis.

Author information

1
Department of Human Genetics, University of Utah, Salt Lake City, UT 84112-5330, USA.
2
Department of Human Genetics, University of Utah, Salt Lake City, UT 84112-5330, USA suzi.mansour@genetics.utah.edu.
3
Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112-5330, USA.

Abstract

Morphogenesis of the inner ear epithelium requires coordinated deployment of several signaling pathways, and disruptions cause abnormalities of hearing and/or balance. The FGFR2b ligands FGF3 and FGF10 are expressed throughout otic development and are required individually for normal morphogenesis, but their prior and redundant roles in otic placode induction complicates investigation of subsequent combinatorial functions in morphogenesis. To interrogate these roles and identify new effectors of FGF3 and FGF10 signaling at the earliest stages of otic morphogenesis, we used conditional gene ablation after otic placode induction, and temporal inhibition of signaling with a secreted, dominant-negative FGFR2b ectodomain. We show that both ligands are required continuously after otocyst formation for maintenance of otic neuroblasts and for patterning and proliferation of the epithelium, leading to normal morphogenesis of both the cochlear and vestibular domains. Furthermore, the first genome-wide identification of proximal targets of FGFR2b signaling in the early otocyst reveals novel candidate genes for inner ear development and function.

KEYWORDS:

Conditional mutant; Ligand trap; Otocyst; RNA-Seq

PMID:
30504125
PMCID:
PMC6307884
[Available on 2019-12-15]
DOI:
10.1242/dev.170142
[Indexed for MEDLINE]
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