Sort by
Items per page

Send to

Choose Destination

Search results

Items: 1 to 50 of 56


Generating mouse models for biomedical research: technological advances.

Gurumurthy CB, Lloyd KCK.

Dis Model Mech. 2019 Jan 8;12(1). pii: dmm029462. doi: 10.1242/dmm.029462. Review.


Human-like NSG mouse glycoproteins sialylation pattern changes the phenotype of human lymphocytes and sensitivity to HIV-1 infection.

Dagur RS, Branch-Woods A, Mathews S, Joshi PS, Quadros RM, Harms DW, Cheng Y, Miles SM, Pirruccello SJ, Gurumurthy CB, Gorantla S, Poluektova LY.

BMC Immunol. 2019 Jan 7;20(1):2. doi: 10.1186/s12865-018-0279-3.


Subcellular localization of sterol biosynthesis enzymes.

Koczok K, Gurumurthy CB, Balogh I, Korade Z, Mirnics K.

J Mol Histol. 2019 Feb;50(1):63-73. doi: 10.1007/s10735-018-9807-y. Epub 2018 Dec 8.


Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line.

Sakurai T, Kamiyoshi A, Ohtsuka M, Gurumurthy CB, Sato M, Shindo T.

Methods Mol Biol. 2019;1874:257-271. doi: 10.1007/978-1-4939-8831-0_15.


Neuron-Type Specific Loss of CDKL5 Leads to Alterations in mTOR Signaling and Synaptic Markers.

Schroeder E, Yuan L, Seong E, Ligon C, DeKorver N, Gurumurthy CB, Arikkath J.

Mol Neurobiol. 2018 Oct 4. doi: 10.1007/s12035-018-1346-8. [Epub ahead of print]


Reprogramming human T cell function and specificity with non-viral genome targeting.

Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta-Aranburu G, Del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A.

Nature. 2018 Jul;559(7714):405-409. doi: 10.1038/s41586-018-0326-5. Epub 2018 Jul 11.


In vivo genome editing targeted towards the female reproductive system.

Sato M, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Gurumurthy CB.

Arch Pharm Res. 2018 Sep;41(9):898-910. doi: 10.1007/s12272-018-1053-z. Epub 2018 Jul 4. Review.


i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.

Ohtsuka M, Sato M, Miura H, Takabayashi S, Matsuyama M, Koyano T, Arifin N, Nakamura S, Wada K, Gurumurthy CB.

Genome Biol. 2018 Feb 26;19(1):25. doi: 10.1186/s13059-018-1400-x.


Antiretroviral Drug Metabolism in Humanized PXR-CAR-CYP3A-NOG Mice.

McMillan JM, Cobb DA, Lin Z, Banoub MG, Dagur RS, Branch Woods AA, Wang W, Makarov E, Kocher T, Joshi PS, Quadros RM, Harms DW, Cohen SM, Gendelman HE, Gurumurthy CB, Gorantla S, Poluektova LY.

J Pharmacol Exp Ther. 2018 May;365(2):272-280. doi: 10.1124/jpet.117.247288. Epub 2018 Feb 23.


Precise and efficient nucleotide substitution near genomic nick via noncanonical homology-directed repair.

Nakajima K, Zhou Y, Tomita A, Hirade Y, Gurumurthy CB, Nakada S.

Genome Res. 2018 Feb;28(2):223-230. doi: 10.1101/gr.226027.117. Epub 2017 Dec 22.


Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors.

Miura H, Quadros RM, Gurumurthy CB, Ohtsuka M.

Nat Protoc. 2018 Jan;13(1):195-215. doi: 10.1038/nprot.2017.153. Epub 2017 Dec 21.


Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior.

Jung EM, Moffat JJ, Liu J, Dravid SM, Gurumurthy CB, Kim WY.

Nat Neurosci. 2017 Dec;20(12):1694-1707. doi: 10.1038/s41593-017-0013-0. Epub 2017 Nov 6.


Mammalian ECD Protein Is a Novel Negative Regulator of the PERK Arm of the Unfolded Protein Response.

Olou AA, Sarkar A, Bele A, Gurumurthy CB, Mir RA, Ammons SA, Mirza S, Saleem I, Urano F, Band H, Band V.

Mol Cell Biol. 2017 Aug 28;37(18). pii: e00030-17. doi: 10.1128/MCB.00030-17. Print 2017 Sep 15.


Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.

Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T, Redder R, Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL, Ohtsuka M, Gurumurthy CB.

Genome Biol. 2017 May 17;18(1):92. doi: 10.1186/s13059-017-1220-4.


Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes.

Jacobi AM, Rettig GR, Turk R, Collingwood MA, Zeiner SA, Quadros RM, Harms DW, Bonthuis PJ, Gregg C, Ohtsuka M, Gurumurthy CB, Behlke MA.

Methods. 2017 May 15;121-122:16-28. doi: 10.1016/j.ymeth.2017.03.021. Epub 2017 Mar 27.


To cleave or not to cleave: therapeutic gene editing with and without programmable nucleases.

Woolf TM, Gurumurthy CB, Boyce F, Kmiec EB.

Nat Rev Drug Discov. 2017 Apr;16(4):296. doi: 10.1038/nrd.2017.42. Epub 2017 Mar 17. No abstract available.


Pronuclear Injection-Based Targeted Transgenesis.

Schilit SL, Ohtsuka M, Quadros RM, Gurumurthy CB.

Curr Protoc Hum Genet. 2016 Oct 11;91:15.10.1-15.10.28. doi: 10.1002/cphg.23.


CRISPR: a versatile tool for both forward and reverse genetics research.

Gurumurthy CB, Grati M, Ohtsuka M, Schilit SL, Quadros RM, Liu XZ.

Hum Genet. 2016 Sep;135(9):971-6. doi: 10.1007/s00439-016-1704-4. Epub 2016 Jul 7.


Cleavage by Caspase 8 and Mitochondrial Membrane Association Activate the BH3-only Protein Bid during TRAIL-induced Apoptosis.

Huang K, Zhang J, O'Neill KL, Gurumurthy CB, Quadros RM, Tu Y, Luo X.

J Biol Chem. 2016 May 27;291(22):11843-51. doi: 10.1074/jbc.M115.711051. Epub 2016 Apr 6.


Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new.

Sato M, Ohtsuka M, Watanabe S, Gurumurthy CB.

Biol Direct. 2016 Mar 31;11(1):16. doi: 10.1186/s13062-016-0115-8. Review.


Simple and reliable genotyping protocol for mouse Prkdc(SCID) mutation.

Quadros RM, Poluektova LY, Gurumurthy CB.

J Immunol Methods. 2016 Apr;431:60-2. doi: 10.1016/j.jim.2016.02.002. Epub 2016 Feb 3.


GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos.

Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M.

Curr Protoc Hum Genet. 2016 Jan 1;88:Unit 15.8. doi: 10.1002/0471142905.hg1508s88.


A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression.

Mir RA, Bele A, Mirza S, Srivastava S, Olou AA, Ammons SA, Kim JH, Gurumurthy CB, Qiu F, Band H, Band V.

Mol Cell Biol. 2015 Dec 28;36(6):886-99. doi: 10.1128/MCB.00594-15.


Mice deficient in Muc4 are resistant to experimental colitis and colitis-associated colorectal cancer.

Das S, Rachagani S, Sheinin Y, Smith LM, Gurumurthy CB, Roy HK, Batra SK.

Oncogene. 2016 May 19;35(20):2645-54. doi: 10.1038/onc.2015.327. Epub 2015 Sep 14.


Assessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice.

Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato M, Ohtsuka M.

PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015.


CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA.

Miura H, Gurumurthy CB, Sato T, Sato M, Ohtsuka M.

Sci Rep. 2015 Aug 5;5:12799. doi: 10.1038/srep12799.


GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice.

Takahashi G, Gurumurthy CB, Wada K, Miura H, Sato M, Ohtsuka M.

Sci Rep. 2015 Jun 22;5:11406. doi: 10.1038/srep11406.


One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).

Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB.

BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.


Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system.

Quadros RM, Harms DW, Ohtsuka M, Gurumurthy CB.

FEBS Open Bio. 2015 Mar 10;5:191-7. doi: 10.1016/j.fob.2015.03.003. eCollection 2015.


Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening.

Gurumurthy CB, Joshi PS, Kurz SG, Ohtsuka M, Quadros RM, Harms DW, Lloyd KC.

Int J Genomics. 2015;2015:735845. doi: 10.1155/2015/735845. Epub 2015 Feb 28.


Generation of a Retinoblastoma (Rb)1-inducible dominant-negative (DN) mouse model.

Tarang S, Doi SM, Gurumurthy CB, Harms D, Quadros R, Rocha-Sanchez SM.

Front Cell Neurosci. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052. eCollection 2015.


The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells.

Bele A, Mirza S, Zhang Y, Ahmad Mir R, Lin S, Kim JH, Gurumurthy CB, West W, Qiu F, Band H, Band V.

Cell Cycle. 2015;14(7):990-1000. doi: 10.1080/15384101.2015.1006982.


Mouse Genome Editing Using the CRISPR/Cas System.

Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB.

Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-27. doi: 10.1002/0471142905.hg1507s83.


Generation of conditional knockout mice.

Sakamoto K, Gurumurthy CB, Wagner KU.

Methods Mol Biol. 2014;1194:21-35. doi: 10.1007/978-1-4939-1215-5_2.


Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination.

Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H.

Transgenic Res. 2013 Aug;22(4):873-5. doi: 10.1007/s11248-013-9703-x. Epub 2013 Mar 26. No abstract available.


Cytoplasmic localization of alteration/deficiency in activation 3 (ADA3) predicts poor clinical outcome in breast cancer patients.

Mirza S, Rakha EA, Alshareeda A, Mohibi S, Zhao X, Katafiasz BJ, Wang J, Gurumurthy CB, Bele A, Ellis IO, Green AR, Band H, Band V.

Breast Cancer Res Treat. 2013 Feb;137(3):721-31. doi: 10.1007/s10549-012-2363-3. Epub 2013 Jan 4.


PITT: pronuclear injection-based targeted transgenesis, a reliable transgene expression method in mice.

Ohtsuka M, Miura H, Sato M, Kimura M, Inoko H, Gurumurthy CB.

Exp Anim. 2012;61(5):489-502. Review.


Alteration/deficiency in activation-3 (Ada3) plays a critical role in maintaining genomic stability.

Mirza S, Katafiasz BJ, Kumar R, Wang J, Mohibi S, Jain S, Gurumurthy CB, Pandita TK, Dave BJ, Band H, Band V.

Cell Cycle. 2012 Nov 15;11(22):4266-74. doi: 10.4161/cc.22613. Epub 2012 Oct 24.


Nucleic acid and non-nucleic acid-based reprogramming of adult limbal progenitors to pluripotency.

Parameswaran S, Balasubramanian S, Babai N, DelDebbio CB, Harms DW, Gurumurthy CB, Rao MS, Sharp JG, Ahmad I.

PLoS One. 2012;7(10):e46734. doi: 10.1371/journal.pone.0046734. Epub 2012 Oct 8.


Overexpression of ecdysoneless in pancreatic cancer and its role in oncogenesis by regulating glycolysis.

Dey P, Rachagani S, Chakraborty S, Singh PK, Zhao X, Gurumurthy CB, Anderson JM, Lele S, Hollingsworth MA, Band V, Batra SK.

Clin Cancer Res. 2012 Nov 15;18(22):6188-98. doi: 10.1158/1078-0432.CCR-12-1789. Epub 2012 Sep 12.


Fluorescent transgenic mice suitable for multi-color aggregation chimera studies.

Ohtsuka M, Miura H, Gurumurthy CB, Kimura M, Inoko H, Yoshimura S, Sato M.

Cell Tissue Res. 2012 Nov;350(2):251-60. doi: 10.1007/s00441-012-1470-0. Epub 2012 Aug 7.


Mammalian alteration/deficiency in activation 3 (Ada3) is essential for embryonic development and cell cycle progression.

Mohibi S, Gurumurthy CB, Nag A, Wang J, Mirza S, Mian Y, Quinn M, Katafiasz B, Eudy J, Pandey S, Guda C, Naramura M, Band H, Band V.

J Biol Chem. 2012 Aug 24;287(35):29442-56. doi: 10.1074/jbc.M112.378901. Epub 2012 Jun 26.


Overexpression of a novel cell cycle regulator ecdysoneless in breast cancer: a marker of poor prognosis in HER2/neu-overexpressing breast cancer patients.

Zhao X, Mirza S, Alshareeda A, Zhang Y, Gurumurthy CB, Bele A, Kim JH, Mohibi S, Goswami M, Lele SM, West W, Qiu F, Ellis IO, Rakha EA, Green AR, Band H, Band V.

Breast Cancer Res Treat. 2012 Jul;134(1):171-80. doi: 10.1007/s10549-011-1946-8. Epub 2012 Jan 22.


Breast cancer subtypes: two decades of journey from cell culture to patients.

Zhao X, Gurumurthy CB, Malhotra G, Mirza S, Mohibi S, Bele A, Quinn MG, Band H, Band V.

Adv Exp Med Biol. 2011;720:135-44. doi: 10.1007/978-1-4614-0254-1_11. Review.


Renal thrombotic microangiopathy in mice with combined deletion of endocytic recycling regulators EHD3 and EHD4.

George M, Rainey MA, Naramura M, Foster KW, Holzapfel MS, Willoughby LL, Ying G, Goswami RM, Gurumurthy CB, Band V, Satchell SC, Band H.

PLoS One. 2011 Mar 9;6(3):e17838. doi: 10.1371/journal.pone.0017838.


Biochemical characterization of human Ecdysoneless reveals a role in transcriptional regulation.

Kim JH, Gurumurthy CB, Band H, Band V.

Biol Chem. 2010 Jan;391(1):9-19. doi: 10.1515/BC.2010.004.


Role of mammalian Ecdysoneless in cell cycle regulation.

Kim JH, Gurumurthy CB, Naramura M, Zhang Y, Dudley AT, Doglio L, Band H, Band V.

J Biol Chem. 2009 Sep 25;284(39):26402-10. doi: 10.1074/jbc.M109.030551. Epub 2009 Jul 29.


Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants.

Solomon A, Mian Y, Ortega-Cava C, Liu VW, Gurumurthy CB, Naramura M, Band V, Band H.

Dev Biol. 2008 Apr 15;316(2):191-9. doi: 10.1016/j.ydbio.2007.12.046. Epub 2008 Jan 11.


The notch regulator MAML1 interacts with p53 and functions as a coactivator.

Zhao Y, Katzman RB, Delmolino LM, Bhat I, Zhang Y, Gurumurthy CB, Germaniuk-Kurowska A, Reddi HV, Solomon A, Zeng MS, Kung A, Ma H, Gao Q, Dimri G, Stanculescu A, Miele L, Wu L, Griffin JD, Wazer DE, Band H, Band V.

J Biol Chem. 2007 Apr 20;282(16):11969-81. Epub 2007 Feb 22.


An essential role of human Ada3 in p53 acetylation.

Nag A, Germaniuk-Kurowska A, Dimri M, Sassack MA, Gurumurthy CB, Gao Q, Dimri G, Band H, Band V.

J Biol Chem. 2007 Mar 23;282(12):8812-20. Epub 2007 Feb 1.

Supplemental Content

Loading ...
Support Center