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Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein.

Oberbichler E, Wiesauer M, Schlögl E, Stangl J, Faschinger F, Knör G, Gruber HJ, Hytönen VP.

Methods Enzymol. 2020;633:1-20. doi: 10.1016/bs.mie.2019.10.028. Epub 2019 Nov 20.


AFM-Based Force Spectroscopy Guided by Recognition Imaging: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density.

Koehler M, Fis A, Gruber HJ, Hinterdorfer P.

Methods Protoc. 2019 Jan 8;2(1). pii: E6. doi: 10.3390/mps2010006.


Subcutaneous adipose tissue distribution and telomere length.

Mangge H, Renner W, Almer G, Gruber HJ, Zelzer S, Moeller R, Horejsi R, Herrmann M.

Clin Chem Lab Med. 2019 Aug 27;57(9):1358-1363. doi: 10.1515/cclm-2018-0801.


Functionalization of AFM Tips and Supports for Molecular Recognition Force Spectroscopy and Recognition Imaging.

Ebner A, Wildling L, Gruber HJ.

Methods Mol Biol. 2019;1886:117-151. doi: 10.1007/978-1-4939-8894-5_7.


Relative telomere length and prostate cancer mortality.

Renner W, Krenn-Pilko S, Gruber HJ, Herrmann M, Langsenlehner T.

Prostate Cancer Prostatic Dis. 2018 Nov;21(4):579-583. doi: 10.1038/s41391-018-0068-3. Epub 2018 Aug 6.


Progesterone-associated arginine decline at luteal phase of menstrual cycle and associations with related amino acids and nuclear factor kB activation.

Faustmann G, Meinitzer A, Magnes C, Tiran B, Obermayer-Pietsch B, Gruber HJ, Ribalta J, Rock E, Roob JM, Winklhofer-Roob BM.

PLoS One. 2018 Jul 10;13(7):e0200489. doi: 10.1371/journal.pone.0200489. eCollection 2018.


Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing.

Zhu R, Gruber HJ, Hinterdorfer P.

Methods Mol Biol. 2018;1814:19-33. doi: 10.1007/978-1-4939-8591-3_2.


Lysyl oxidase-like protein 2 (LOXL2) modulates barrier function in cholangiocytes in cholestasis.

Pollheimer MJ, Racedo S, Mikels-Vigdal A, Marshall D, Bowlus C, Lackner C, Madl T, Karlsen TH, Hov JR, Lyman SK, Adamkewicz J, Smith V, Moreau E, Zollner G, Eide TJ, Stojakovic T, Scharnagl H, Gruber HJ, Stauber RE, Trauner M, Fickert P.

J Hepatol. 2018 Aug;69(2):368-377. doi: 10.1016/j.jhep.2018.04.009. Epub 2018 Apr 28.


Communication between N terminus and loop2 tunes Orai activation.

Fahrner M, Pandey SK, Muik M, Traxler L, Butorac C, Stadlbauer M, Zayats V, Krizova A, Plenk P, Frischauf I, Schindl R, Gruber HJ, Hinterdorfer P, Ettrich R, Romanin C, Derler I.

J Biol Chem. 2018 Jan 26;293(4):1271-1285. doi: 10.1074/jbc.M117.812693. Epub 2017 Dec 13.


Stable Europium(III) Complexes with Short Linkers for Site-Specific Labeling of Biomolecules.

Faschinger F, Ertl M, Zimmermann M, Horner A, Himmelsbach M, Schöfberger W, Knör G, Gruber HJ.

ChemistryOpen. 2017 Sep 4;6(6):721-732. doi: 10.1002/open.201700122. eCollection 2017 Dec.


Detailed Evidence for an Unparalleled Interaction Mode between Calmodulin and Orai Proteins.

Traxler L, Rathner P, Fahrner M, Stadlbauer M, Faschinger F, Charnavets T, Müller N, Romanin C, Hinterdorfer P, Gruber HJ.

Angew Chem Int Ed Engl. 2017 Dec 4;56(49):15755-15759. doi: 10.1002/anie.201708667. Epub 2017 Nov 6.


Regenerative biosensor for use with biotinylated bait molecules.

Knoglinger C, Zich A, Traxler L, Poslední K, Friedl G, Ruttmann B, Schorpp A, Müller K, Zimmermann M, Gruber HJ.

Biosens Bioelectron. 2018 Jan 15;99:684-690. doi: 10.1016/j.bios.2017.07.033. Epub 2017 Jul 13.


Combined Recognition Imaging and Force Spectroscopy: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density.

Koehler M, Macher G, Rupprecht A, Zhu R, Gruber HJ, Pohl EE, Hinterdorfer P.

Sci Adv Mater. 2017 Jan 1;9(1):128-134. doi: 10.1166/sam.2017.3066.


Curli mediate bacterial adhesion to fibronectin via tensile multiple bonds.

Oh YJ, Hubauer-Brenner M, Gruber HJ, Cui Y, Traxler L, Siligan C, Park S, Hinterdorfer P.

Sci Rep. 2016 Sep 22;6:33909. doi: 10.1038/srep33909.


Single molecule force spectroscopy data and BD- and MD simulations on the blood protein von Willebrand factor.

Posch S, Aponte-Santamaría C, Schwarzl R, Karner A, Radtke M, Gräter F, Obser T, König G, Brehm MA, Gruber HJ, Netz RR, Baldauf C, Schneppenheim R, Tampé R, Hinterdorfer P.

Data Brief. 2016 Jul 21;8:1080-7. doi: 10.1016/j.dib.2016.07.031. eCollection 2016 Sep.


Mutual A domain interactions in the force sensing protein von Willebrand factor.

Posch S, Aponte-Santamaría C, Schwarzl R, Karner A, Radtke M, Gräter F, Obser T, König G, Brehm MA, Gruber HJ, Netz RR, Baldauf C, Schneppenheim R, Tampé R, Hinterdorfer P.

J Struct Biol. 2017 Jan;197(1):57-64. doi: 10.1016/j.jsb.2016.04.012. Epub 2016 Apr 23.


Circulating leptin and NF-κB activation in peripheral blood mononuclear cells across the menstrual cycle.

Faustmann G, Tiran B, Maimari T, Kieslinger P, Obermayer-Pietsch B, Gruber HJ, Roob JM, Winklhofer-Roob BM.

Biofactors. 2016 Jul 8;42(4):376-87. doi: 10.1002/biof.1281. Epub 2016 Apr 20.


Nanopharmacological Force Sensing to Reveal Allosteric Coupling in Transporter Binding Sites.

Zhu R, Sinwel D, Hasenhuetl PS, Saha K, Kumar V, Zhang P, Rankl C, Holy M, Sucic S, Kudlacek O, Karner A, Sandtner W, Stockner T, Gruber HJ, Freissmuth M, Newman AH, Sitte HH, Hinterdorfer P.

Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1719-22. doi: 10.1002/anie.201508755. Epub 2015 Dec 22.


Hypothyroidism Exacerbates Thrombophilia in Female Rats Fed with a High Fat Diet.

Mangge H, Prüller F, Zelzer S, Ainödhofer H, Pailer S, Kieslinger P, Haybaeck J, Obermayer-Pietsch B, Cvirn G, Gruber HJ.

Int J Mol Sci. 2015 Jul 10;16(7):15776-84. doi: 10.3390/ijms160715776.


Oxidative stress: Determination of 4-hydroxy-2-nonenal by gas chromatography/mass spectrometry in human and rat plasma.

Zelzer S, Mangge H, Oberreither R, Bernecker C, Gruber HJ, Prüller F, Fauler G.

Free Radic Res. 2015 Oct;49(10):1233-8. doi: 10.3109/10715762.2015.1059936. Epub 2015 Aug 11.


Oxidized LDL Is Strictly Limited to Hyperthyroidism Irrespective of Fat Feeding in Female Sprague Dawley Rats.

Zelzer S, Mangge H, Pailer S, Ainoedhofer H, Kieslinger P, Stojakovic T, Scharnagl H, Prüller F, Weghuber D, Datz C, Haybaeck J, Obermayer-Pietsch B, Trummer C, Gostner J, Gruber HJ.

Int J Mol Sci. 2015 May 21;16(5):11689-98. doi: 10.3390/ijms160511689.


Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria.

Graupp M, Gruber HJ, Weiss G, Kiesler K, Bachna-Rotter S, Friedrich G, Gugatschka M.

Laryngoscope. 2015 Jun;125(6):E203-9. doi: 10.1002/lary.25103. Epub 2014 Dec 29.


Ultra-endurance exercise induces stress and inflammation and affects circulating hematopoietic progenitor cell function.

Stelzer I, Kröpfl JM, Fuchs R, Pekovits K, Mangge H, Raggam RB, Gruber HJ, Prüller F, Hofmann P, Truschnig-Wilders M, Obermayer-Pietsch B, Haushofer AC, Kessler HH, Mächler P.

Scand J Med Sci Sports. 2015 Oct;25(5):e442-50. doi: 10.1111/sms.12347. Epub 2014 Dec 1.


Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity.

Taskinen B, Zauner D, Lehtonen SI, Koskinen M, Thomson C, Kähkönen N, Kukkurainen S, Määttä JA, Ihalainen TO, Kulomaa MS, Gruber HJ, Hytönen VP.

Bioconjug Chem. 2014 Dec 17;25(12):2233-43. doi: 10.1021/bc500462w. Epub 2014 Dec 1.


IgGs are made for walking on bacterial and viral surfaces.

Preiner J, Kodera N, Tang J, Ebner A, Brameshuber M, Blaas D, Gelbmann N, Gruber HJ, Ando T, Hinterdorfer P.

Nat Commun. 2014 Jul 10;5:4394. doi: 10.1038/ncomms5394.


Forces and dynamics of glucose and inhibitor binding to sodium glucose co-transporter SGLT1 studied by single molecule force spectroscopy.

Neundlinger I, Puntheeranurak T, Wildling L, Rankl C, Wang LX, Gruber HJ, Kinne RK, Hinterdorfer P.

J Biol Chem. 2014 Aug 1;289(31):21673-83. doi: 10.1074/jbc.M113.529875. Epub 2014 Jun 24.


Vocal fold fibroblast response to growth factor treatment is age dependent: results from an in vitro study.

Graupp M, Kiesler K, Friedrich G, Ainödhofer H, Gruber HJ, Kieslinger P, Saxena A, Hirano S, Gugatschka M.

J Voice. 2014 Jul;28(4):420-3. doi: 10.1016/j.jvoice.2013.11.005. Epub 2014 Feb 1.


Investigating the binding behaviour of two avidin-based testosterone binders using molecular recognition force spectroscopy.

Rangl M, Leitner M, Riihimäki T, Lehtonen S, Hytönen VP, Gruber HJ, Kulomaa M, Hinterdorfer P, Ebner A.

J Mol Recognit. 2014 Feb;27(2):92-7. doi: 10.1002/jmr.2337.


Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay.

Näreoja T, Ebner A, Gruber HJ, Taskinen B, Kienberger F, Hänninen PE, Hytönen VP, Hinterdorfer P, Härmä H.

Anal Bioanal Chem. 2014 Jan;406(2):493-503. doi: 10.1007/s00216-013-7474-0. Epub 2013 Nov 22.


Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats.

Gugatschka M, Ainödhofer H, Gruber HJ, Graupp M, Kieslinger P, Kiesler K, Saxena A, Hirano S, Friedrich G.

Eur Arch Otorhinolaryngol. 2014 May;271(5):1107-12.


Single-molecule analysis of the recognition forces underlying nucleo-cytoplasmic transport.

Rangl M, Ebner A, Yamada J, Rankl C, Tampé R, Gruber HJ, Rexach M, Hinterdorfer P.

Angew Chem Int Ed Engl. 2013 Sep 23;52(39):10356-9. doi: 10.1002/anie.201305359. Epub 2013 Sep 5. No abstract available.


Reversible biofunctionalization of surfaces with a switchable mutant of avidin.

Pollheimer P, Taskinen B, Scherfler A, Gusenkov S, Creus M, Wiesauer P, Zauner D, Schöfberger W, Schwarzinger C, Ebner A, Tampé R, Stutz H, Hytönen VP, Gruber HJ.

Bioconjug Chem. 2013 Oct 16;24(10):1656-68. doi: 10.1021/bc400087e. Epub 2013 Sep 9.


The extended transmembrane Orai1 N-terminal (ETON) region combines binding interface and gate for Orai1 activation by STIM1.

Derler I, Plenk P, Fahrner M, Muik M, Jardin I, Schindl R, Gruber HJ, Groschner K, Romanin C.

J Biol Chem. 2013 Oct 4;288(40):29025-34. doi: 10.1074/jbc.M113.501510. Epub 2013 Aug 13.


Treatment with the nitric oxide donor SNP increases triiodothyronine levels in hyper- and hypothyroid Sprague-Dawley rats.

Ragginer C, Bernecker C, Ainoedhofer H, Pailer S, Kieslinger P, Truschnig-Wilders M, Gruber HJ.

Horm Metab Res. 2013 Oct;45(11):808-12. doi: 10.1055/s-0033-1349892. Epub 2013 Aug 5.


Mapping the nucleotide binding site of uncoupling protein 1 using atomic force microscopy.

Zhu R, Rupprecht A, Ebner A, Haselgrübler T, Gruber HJ, Hinterdorfer P, Pohl EE.

J Am Chem Soc. 2013 Mar 6;135(9):3640-6. doi: 10.1021/ja312550k. Epub 2013 Feb 26.


Reduced urinary glutamate levels are associated with the frequency of migraine attacks in females.

Ragginer C, Lechner A, Bernecker C, Horejsi R, Möller R, Wallner-Blazek M, Weiss S, Fazekas F, Schmidt R, Truschnig-Wilders M, Gruber HJ.

Eur J Neurol. 2012 Aug;19(8):1146-50. doi: 10.1111/j.1468-1331.2012.03693.x. Epub 2012 Mar 21.


Adult-type hypolactasia and calcium intake in polycystic ovary syndrome.

Lerchbaum E, Giuliani A, Gruber HJ, Pieber TR, Obermayer-Pietsch B.

Clin Endocrinol (Oxf). 2012 Dec;77(6):834-43. doi: 10.1111/j.1365-2265.2012.04334.x.


Active versus passive microleakage of Resilon/Epiphany and gutta-percha/AH Plus.

Kqiku L, Städtler P, Gruber HJ, Baraba A, Anic I, Miletic I.

Aust Endod J. 2011 Dec;37(3):141-6. doi: 10.1111/j.1747-4477.2010.00238.x. Epub 2010 Sep 1.


Nanoscale DNA tetrahedra improve biomolecular recognition on patterned surfaces.

Schlapak R, Danzberger J, Armitage D, Morgan D, Ebner A, Hinterdorfer P, Pollheimer P, Gruber HJ, Schäffler F, Howorka S.

Small. 2012 Jan 9;8(1):89-97. doi: 10.1002/smll.201101576. Epub 2011 Nov 15.


Probing binding pocket of serotonin transporter by single molecular force spectroscopy on living cells.

Wildling L, Rankl C, Haselgrübler T, Gruber HJ, Holy M, Newman AH, Zou MF, Zhu R, Freissmuth M, Sitte HH, Hinterdorfer P.

J Biol Chem. 2012 Jan 2;287(1):105-13. doi: 10.1074/jbc.M111.304873. Epub 2011 Oct 27.


Susceptibility loci for polycystic ovary syndrome on chromosome 2p16.3, 2p21, and 9q33.3 in a cohort of Caucasian women.

Lerchbaum E, Trummer O, Giuliani A, Gruber HJ, Pieber TR, Obermayer-Pietsch B.

Horm Metab Res. 2011 Oct;43(11):743-7. doi: 10.1055/s-0031-1286279. Epub 2011 Oct 18.


Fatty liver index in polycystic ovary syndrome.

Lerchbaum E, Gruber HJ, Schwetz V, Giuliani A, Möller R, Pieber TR, Obermayer-Pietsch B.

Eur J Endocrinol. 2011 Dec;165(6):935-43. doi: 10.1530/EJE-11-0614. Epub 2011 Sep 21.


Single-molecule AFM characterization of individual chemically tagged DNA tetrahedra.

Leitner M, Mitchell N, Kastner M, Schlapak R, Gruber HJ, Hinterdorfer P, Howorka S, Ebner A.

ACS Nano. 2011 Sep 27;5(9):7048-54. doi: 10.1021/nn201705p. Epub 2011 Aug 9.


Molecular determinants within N terminus of Orai3 protein that control channel activation and gating.

Bergsmann J, Derler I, Muik M, Frischauf I, Fahrner M, Pollheimer P, Schwarzinger C, Gruber HJ, Groschner K, Romanin C.

J Biol Chem. 2011 Sep 9;286(36):31565-75. doi: 10.1074/jbc.M111.227546. Epub 2011 Jul 1.


Linking of sensor molecules with amino groups to amino-functionalized AFM tips.

Wildling L, Unterauer B, Zhu R, Rupprecht A, Haselgrübler T, Rankl C, Ebner A, Vater D, Pollheimer P, Pohl EE, Hinterdorfer P, Gruber HJ.

Bioconjug Chem. 2011 Jun 15;22(6):1239-48. doi: 10.1021/bc200099t. Epub 2011 May 4.


Oxidative stress is associated with migraine and migraine-related metabolic risk in females.

Bernecker C, Ragginer C, Fauler G, Horejsi R, Möller R, Zelzer S, Lechner A, Wallner-Blazek M, Weiss S, Fazekas F, Bahadori B, Truschnig-Wilders M, Gruber HJ.

Eur J Neurol. 2011 Oct;18(10):1233-9. doi: 10.1111/j.1468-1331.2011.03414.x. Epub 2011 Apr 23.


The lipid accumulation product is associated with impaired glucose tolerance in PCOS women.

Wehr E, Gruber HJ, Giuliani A, Möller R, Pieber TR, Obermayer-Pietsch B.

J Clin Endocrinol Metab. 2011 Jun;96(6):E986-90. doi: 10.1210/jc.2011-0031. Epub 2011 Apr 6.


Vitamin D-associated polymorphisms are related to insulin resistance and vitamin D deficiency in polycystic ovary syndrome.

Wehr E, Trummer O, Giuliani A, Gruber HJ, Pieber TR, Obermayer-Pietsch B.

Eur J Endocrinol. 2011 May;164(5):741-9. doi: 10.1530/EJE-11-0134. Epub 2011 Mar 9.


Nanomechanical recognition measurements of individual DNA molecules reveal epigenetic methylation patterns.

Zhu R, Howorka S, Pröll J, Kienberger F, Preiner J, Hesse J, Ebner A, Pastushenko VP, Gruber HJ, Hinterdorfer P.

Nat Nanotechnol. 2010 Nov;5(11):788-91. doi: 10.1038/nnano.2010.212. Epub 2010 Oct 31.


GLP-2 and leptin are associated with hyperinsulinemia in non-obese female migraineurs.

Bernecker C, Pailer S, Kieslinger P, Horejsi R, Möller R, Lechner A, Wallner-Blazek M, Weiss S, Fazekas F, Truschnig-Wilders M, Gruber HJ.

Cephalalgia. 2010 Nov;30(11):1366-74. doi: 10.1177/0333102410364674. Epub 2010 Mar 26.


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