Format

Send to

Choose Destination
G3 (Bethesda). 2018 Mar 28;8(4):1139-1145. doi: 10.1534/g3.117.300388.

Maize Transposable Elements Ac/Ds as Insertion Mutagenesis Tools in Candida albicans.

Author information

1
Institute of Biology, Dahlem Centre of Plant Sciences, Free University pf Berlin, 14195, Germany.
2
Department of Molecular Microbiology and Biotechnology, George Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel.
3
Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 138673, Singapore.
4
Institute of Biology, Dahlem Centre of Plant Sciences, Free University pf Berlin, 14195, Germany reinhard.kunze@fu-berlin.de.

Abstract

In nonmodel systems, genetic research is often limited by the lack of techniques for the generation and identification of gene mutations. One approach to overcome this bottleneck is the application of transposons for gene tagging. We have established a two-element transposon tagging system, based on the transposable elements Activator (Ac)/Dissociation (Ds) from maize, for in vivo insertion mutagenesis in the fungal human pathogen Candida albicans A nonautonomous Ds transposon carrying a selectable marker was constructed into the ADE2 promoter on chromosome 3 and a codon usage-adapted Ac transposase gene was inserted into the neutral NEUT5L locus on chromosome 5. In C. albicans cells expressing the transposase, the Ds element efficiently excised and reintegrated elsewhere in the genome, which makes the Ac/Ds transposons promising tools for saturating insertion mutagenesis in clinical strains of C. albicans.

KEYWORDS:

Activator/Dissociation; Candida albicans; Zea mays; insertion mutagenesis; transposon tagging

PMID:
29378819
PMCID:
PMC5873905
DOI:
10.1534/g3.117.300388
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center