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Items: 1 to 50 of 64

1.

DNA assembly with error correction on a droplet digital microfluidics platform.

Khilko Y, Weyman PD, Glass JI, Adams MD, McNeil MA, Griffin PB.

BMC Biotechnol. 2018 Jun 1;18(1):37. doi: 10.1186/s12896-018-0439-9.

2.

Tuning Gene Activity by Inducible and Targeted Regulation of Gene Expression in Minimal Bacterial Cells.

Mariscal AM, Kakizawa S, Hsu JY, Tanaka K, González-González L, Broto A, Querol E, Lluch-Senar M, Piñero-Lambea C, Sun L, Weyman PD, Wise KS, Merryman C, Tse G, Moore AJ, Hutchison CA 3rd, Smith HO, Tomita M, Venter JC, Glass JI, Piñol J, Suzuki Y.

ACS Synth Biol. 2018 Jun 15;7(6):1538-1552. doi: 10.1021/acssynbio.8b00028. Epub 2018 May 22.

3.

Minimal Cells-Real and Imagined.

Glass JI, Merryman C, Wise KS, Hutchison CA 3rd, Smith HO.

Cold Spring Harb Perspect Biol. 2017 Dec 1;9(12). pii: a023861. doi: 10.1101/cshperspect.a023861. Review.

PMID:
28348033
4.

Efficient size-independent chromosome delivery from yeast to cultured cell lines.

Brown DM, Chan YA, Desai PJ, Grzesik P, Oldfield LM, Vashee S, Way JC, Silver PA, Glass JI.

Nucleic Acids Res. 2017 Apr 20;45(7):e50. doi: 10.1093/nar/gkw1252.

5.

One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9.

Kannan K, Tsvetanova B, Chuang RY, Noskov VN, Assad-Garcia N, Ma L, Hutchison Iii CA, Smith HO, Glass JI, Merryman C, Venter JC, Gibson DG.

Sci Rep. 2016 Aug 4;6:30714. doi: 10.1038/srep30714.

6.

Design and synthesis of a minimal bacterial genome.

Hutchison CA 3rd, Chuang RY, Noskov VN, Assad-Garcia N, Deerinck TJ, Ellisman MH, Gill J, Kannan K, Karas BJ, Ma L, Pelletier JF, Qi ZQ, Richter RA, Strychalski EA, Sun L, Suzuki Y, Tsvetanova B, Wise KS, Smith HO, Glass JI, Merryman C, Gibson DG, Venter JC.

Science. 2016 Mar 25;351(6280):aad6253. doi: 10.1126/science.aad6253. Erratum in: ACS Chem Biol. 2016 May 20;11(5):1463.

PMID:
27013737
7.

Bacterial antisense RNAs are mainly the product of transcriptional noise.

Lloréns-Rico V, Cano J, Kamminga T, Gil R, Latorre A, Chen WH, Bork P, Glass JI, Serrano L, Lluch-Senar M.

Sci Adv. 2016 Mar 4;2(3):e1501363. doi: 10.1126/sciadv.1501363. eCollection 2016 Mar.

8.

Designer diatom episomes delivered by bacterial conjugation.

Karas BJ, Diner RE, Lefebvre SC, McQuaid J, Phillips AP, Noddings CM, Brunson JK, Valas RE, Deerinck TJ, Jablanovic J, Gillard JT, Beeri K, Ellisman MH, Glass JI, Hutchison CA 3rd, Smith HO, Venter JC, Allen AE, Dupont CL, Weyman PD.

Nat Commun. 2015 Apr 21;6:6925. doi: 10.1038/ncomms7925.

9.

Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.

Suzuki Y, Assad-Garcia N, Kostylev M, Noskov VN, Wise KS, Karas BJ, Stam J, Montague MG, Hanly TJ, Enriquez NJ, Ramon A, Goldgof GM, Richter RA, Vashee S, Chuang RY, Winzeler EA, Hutchison CA 3rd, Gibson DG, Smith HO, Glass JI, Venter JC.

Genome Res. 2015 Mar;25(3):435-44. doi: 10.1101/gr.182477.114. Epub 2015 Feb 5.

10.

Random insertion and gene disruption via transposon mutagenesis of Ureaplasma parvum using a mini-transposon plasmid.

Aboklaish AF, Dordet-Frisoni E, Citti C, Toleman MA, Glass JI, Spiller OB.

Int J Med Microbiol. 2014 Nov;304(8):1218-25. doi: 10.1016/j.ijmm.2014.09.003. Epub 2014 Sep 26.

11.

Rescue of mutant fitness defects using in vitro reconstituted designer transposons in Mycoplasma mycoides.

Karas BJ, Wise KS, Sun L, Venter JC, Glass JI, Hutchison CA 3rd, Smith HO, Suzuki Y.

Front Microbiol. 2014 Jul 23;5:369. doi: 10.3389/fmicb.2014.00369. eCollection 2014.

12.

Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases.

Krishnakumar R, Grose C, Haft DH, Zaveri J, Alperovich N, Gibson DG, Merryman C, Glass JI.

Nucleic Acids Res. 2014 Aug;42(14):e111. doi: 10.1093/nar/gku509. Epub 2014 Jun 9.

13.

Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.

Karas BJ, Jablanovic J, Irvine E, Sun L, Ma L, Weyman PD, Gibson DG, Glass JI, Venter JC, Hutchison CA 3rd, Smith HO, Suzuki Y.

Nat Protoc. 2014 Apr;9(4):743-50. doi: 10.1038/nprot.2014.045. Epub 2014 Mar 6.

PMID:
24603933
14.

A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.

Grover RK, Zhu X, Nieusma T, Jones T, Boreo I, MacLeod AS, Mark A, Niessen S, Kim HJ, Kong L, Assad-Garcia N, Kwon K, Chesi M, Smider VV, Salomon DR, Jelinek DF, Kyle RA, Pyles RB, Glass JI, Ward AB, Wilson IA, Lerner RA.

Science. 2014 Feb 7;343(6171):656-661. doi: 10.1126/science.1246135.

15.

Assembly of eukaryotic algal chromosomes in yeast.

Karas BJ, Molparia B, Jablanovic J, Hermann WJ, Lin YC, Dupont CL, Tagwerker C, Yonemoto IT, Noskov VN, Chuang RY, Allen AE, Glass JI, Hutchison CA 3rd, Smith HO, Venter JC, Weyman PD.

J Biol Eng. 2013 Dec 10;7(1):30. doi: 10.1186/1754-1611-7-30.

16.

Transfer RNA misidentification scrambles sense codon recoding.

Krishnakumar R, Prat L, Aerni HR, Ling J, Merryman C, Glass JI, Rinehart J, Söll D.

Chembiochem. 2013 Oct 11;14(15):1967-72. doi: 10.1002/cbic.201300444. Epub 2013 Sep 2.

17.

Synthetic generation of influenza vaccine viruses for rapid response to pandemics.

Dormitzer PR, Suphaphiphat P, Gibson DG, Wentworth DE, Stockwell TB, Algire MA, Alperovich N, Barro M, Brown DM, Craig S, Dattilo BM, Denisova EA, De Souza I, Eickmann M, Dugan VG, Ferrari A, Gomila RC, Han L, Judge C, Mane S, Matrosovich M, Merryman C, Palladino G, Palmer GA, Spencer T, Strecker T, Trusheim H, Uhlendorff J, Wen Y, Yee AC, Zaveri J, Zhou B, Becker S, Donabedian A, Mason PW, Glass JI, Rappuoli R, Venter JC.

Sci Transl Med. 2013 May 15;5(185):185ra68. doi: 10.1126/scitranslmed.3006368.

18.

Assembly of large, high G+C bacterial DNA fragments in yeast.

Noskov VN, Karas BJ, Young L, Chuang RY, Gibson DG, Lin YC, Stam J, Yonemoto IT, Suzuki Y, Andrews-Pfannkoch C, Glass JI, Smith HO, Hutchison CA 3rd, Venter JC, Weyman PD.

ACS Synth Biol. 2012 Jul 20;1(7):267-73. doi: 10.1021/sb3000194. Epub 2012 May 21.

PMID:
23651249
19.

Direct transfer of whole genomes from bacteria to yeast.

Karas BJ, Jablanovic J, Sun L, Ma L, Goldgof GM, Stam J, Ramon A, Manary MJ, Winzeler EA, Venter JC, Weyman PD, Gibson DG, Glass JI, Hutchison CA 3rd, Smith HO, Suzuki Y.

Nat Methods. 2013 May;10(5):410-2. doi: 10.1038/nmeth.2433. Epub 2013 Mar 31.

20.

Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.

Tagwerker C, Dupont CL, Karas BJ, Ma L, Chuang RY, Benders GA, Ramon A, Novotny M, Montague MG, Venepally P, Brami D, Schwartz A, Andrews-Pfannkoch C, Gibson DG, Glass JI, Smith HO, Venter JC, Hutchison CA 3rd.

Nucleic Acids Res. 2012 Nov 1;40(20):10375-83. doi: 10.1093/nar/gks823. Epub 2012 Aug 31.

21.

Complete genome sequences of Mycoplasma leachii strain PG50T and the pathogenic Mycoplasma mycoides subsp. mycoides small colony biotype strain Gladysdale.

Wise KS, Calcutt MJ, Foecking MF, Madupu R, DeBoy RT, Röske K, Hvinden ML, Martin TR, Durkin AS, Glass JI, Methé BA.

J Bacteriol. 2012 Aug;194(16):4448-9. doi: 10.1128/JB.00761-12.

22.

Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.

Waites KB, Xiao L, Paralanov V, Viscardi RM, Glass JI.

J Mol Diagn. 2012 Sep;14(5):437-50. doi: 10.1016/j.jmoldx.2012.06.001. Epub 2012 Jul 20. Review.

23.

A whole-cell computational model predicts phenotype from genotype.

Karr JR, Sanghvi JC, Macklin DN, Gutschow MV, Jacobs JM, Bolival B Jr, Assad-Garcia N, Glass JI, Covert MW.

Cell. 2012 Jul 20;150(2):389-401. doi: 10.1016/j.cell.2012.05.044.

24.

RecA mediates MgpB and MgpC phase and antigenic variation in Mycoplasma genitalium, but plays a minor role in DNA repair.

Burgos R, Wood GE, Young L, Glass JI, Totten PA.

Mol Microbiol. 2012 Aug;85(4):669-83. doi: 10.1111/j.1365-2958.2012.08130.x. Epub 2012 Jul 5.

25.

Comparative genome analysis of 19 Ureaplasma urealyticum and Ureaplasma parvum strains.

Paralanov V, Lu J, Duffy LB, Crabb DM, Shrivastava S, Methé BA, Inman J, Yooseph S, Xiao L, Cassell GH, Waites KB, Glass JI.

BMC Microbiol. 2012 May 30;12:88. doi: 10.1186/1471-2180-12-88.

26.

The costimulatory immunogen LPS induces the B-Cell clones that infiltrate transplanted human kidneys.

Grover RK, Cheng J, Peng Y, Jones TM, Ruiz DI, Ulevitch RJ, Glass JI, Dennis EA, Salomon DR, Lerner RA.

Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6036-41. doi: 10.1073/pnas.1202214109. Epub 2012 Apr 6. Erratum in: Proc Natl Acad Sci U S A. 2016 Oct 17;:.

27.

Chromosomal mutations responsible for fluoroquinolone resistance in Ureaplasma species in the United States.

Xiao L, Crabb DM, Duffy LB, Paralanov V, Glass JI, Waites KB.

Antimicrob Agents Chemother. 2012 May;56(5):2780-3. doi: 10.1128/AAC.06342-11. Epub 2012 Feb 27.

28.

Synthetic genomics and the construction of a synthetic bacterial cell.

Glass JI.

Perspect Biol Med. 2012;55(4):473-89. doi: 10.1353/pbm.2012.0040.

PMID:
23502559
29.

Essence of life: essential genes of minimal genomes.

Juhas M, Eberl L, Glass JI.

Trends Cell Biol. 2011 Oct;21(10):562-8. doi: 10.1016/j.tcb.2011.07.005. Epub 2011 Sep 1.

PMID:
21889892
30.

Genotypic characterization of ureaplasma serovars from clinical isolates by pulsed-field gel electrophoresis.

Xiao L, Crabb DM, Moser SA, Duffy LB, Glass JI, Paralanov V, Waites KB.

J Clin Microbiol. 2011 Sep;49(9):3325-8. doi: 10.1128/JCM.01235-11. Epub 2011 Jul 27.

31.

Extensive horizontal gene transfer in ureaplasmas from humans questions the utility of serotyping for diagnostic purposes.

Xiao L, Paralanov V, Glass JI, Duffy LB, Robertson JA, Cassell GH, Chen Y, Waites KB.

J Clin Microbiol. 2011 Aug;49(8):2818-26. doi: 10.1128/JCM.00637-11. Epub 2011 Jun 22.

32.

Genome sequences of Mycoplasma alligatoris A21JP2T and Mycoplasma crocodyli MP145T.

Brown DR, Farmerie WG, May M, Benders GA, Durkin AS, Hlavinka K, Hostetler J, Jackson J, Johnson J, Miller RH, Paralanov V, Radune D, Szczypinski B, Glass JI.

J Bacteriol. 2011 Jun;193(11):2892-3. doi: 10.1128/JB.00309-11. Epub 2011 Apr 1.

33.

Mutations in ribosomal proteins and ribosomal RNA confer macrolide resistance in human Ureaplasma spp.

Xiao L, Crabb DM, Duffy LB, Paralanov V, Glass JI, Hamilos DL, Waites KB.

Int J Antimicrob Agents. 2011 Apr;37(4):377-9. doi: 10.1016/j.ijantimicag.2010.12.012. Epub 2011 Feb 24.

PMID:
21353494
34.

Enhancement of targeted homologous recombination in Mycoplasma mycoides subsp. capri by inclusion of heterologous recA.

Allam AB, Reyes L, Assad-Garcia N, Glass JI, Brown MB.

Appl Environ Microbiol. 2010 Oct;76(20):6951-4. doi: 10.1128/AEM.00056-10. Epub 2010 Aug 27.

35.

A versatile palindromic amphipathic repeat coding sequence horizontally distributed among diverse bacterial and eucaryotic microbes.

Röske K, Foecking MF, Yooseph S, Glass JI, Calcutt MJ, Wise KS.

BMC Genomics. 2010 Jul 13;11:430. doi: 10.1186/1471-2164-11-430.

36.

Detection and characterization of human Ureaplasma species and serovars by real-time PCR.

Xiao L, Glass JI, Paralanov V, Yooseph S, Cassell GH, Duffy LB, Waites KB.

J Clin Microbiol. 2010 Aug;48(8):2715-23. doi: 10.1128/JCM.01877-09. Epub 2010 Jun 16.

37.

Targeted chromosomal knockouts in Mycoplasma pneumoniae.

Krishnakumar R, Assad-Garcia N, Benders GA, Phan Q, Montague MG, Glass JI.

Appl Environ Microbiol. 2010 Aug;76(15):5297-9. doi: 10.1128/AEM.00024-10. Epub 2010 Jun 11.

38.

Creation of a bacterial cell controlled by a chemically synthesized genome.

Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC.

Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20.

39.

Cloning whole bacterial genomes in yeast.

Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, Hutchison CA 3rd.

Nucleic Acids Res. 2010 May;38(8):2558-69. doi: 10.1093/nar/gkq119. Epub 2010 Mar 7.

40.

A systems biology tour de force for a near-minimal bacterium.

Glass JI, Hutchison CA 3rd, Smith HO, Venter JC.

Mol Syst Biol. 2009;5:330. doi: 10.1038/msb.2009.89. Epub 2009 Dec 1. No abstract available.

41.

Creating bacterial strains from genomes that have been cloned and engineered in yeast.

Lartigue C, Vashee S, Algire MA, Chuang RY, Benders GA, Ma L, Noskov VN, Denisova EA, Gibson DG, Assad-Garcia N, Alperovich N, Thomas DW, Merryman C, Hutchison CA 3rd, Smith HO, Venter JC, Glass JI.

Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20.

42.

New selectable marker for manipulating the simple genomes of Mycoplasma species.

Algire MA, Lartigue C, Thomas DW, Assad-Garcia N, Glass JI, Merryman C.

Antimicrob Agents Chemother. 2009 Oct;53(10):4429-32. doi: 10.1128/AAC.00388-09. Epub 2009 Aug 17.

43.

A genome-scale metabolic reconstruction of Mycoplasma genitalium, iPS189.

Suthers PF, Dasika MS, Kumar VS, Denisov G, Glass JI, Maranas CD.

PLoS Comput Biol. 2009 Feb;5(2):e1000285. doi: 10.1371/journal.pcbi.1000285. Epub 2009 Feb 13.

44.

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.

Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, Smith HO.

Science. 2008 Feb 29;319(5867):1215-20. doi: 10.1126/science.1151721. Epub 2008 Jan 24.

45.

The Sorcerer II Global Ocean Sampling Expedition: metagenomic characterization of viruses within aquatic microbial samples.

Williamson SJ, Rusch DB, Yooseph S, Halpern AL, Heidelberg KB, Glass JI, Andrews-Pfannkoch C, Fadrosh D, Miller CS, Sutton G, Frazier M, Venter JC.

PLoS One. 2008 Jan 23;3(1):e1456. doi: 10.1371/journal.pone.0001456.

46.

Genome transplantation in bacteria: changing one species to another.

Lartigue C, Glass JI, Alperovich N, Pieper R, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC.

Science. 2007 Aug 3;317(5838):632-8. Epub 2007 Jun 28.

47.

Cytokine-activated natural killer cells exert direct killing of hepatoma cells harboring hepatitis C virus replicons.

Larkin J, Bost A, Glass JI, Tan SL.

J Interferon Cytokine Res. 2006 Dec;26(12):854-65.

PMID:
17238828
48.

Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6.

Lanie JA, Ng WL, Kazmierczak KM, Andrzejewski TM, Davidsen TM, Wayne KJ, Tettelin H, Glass JI, Winkler ME.

J Bacteriol. 2007 Jan;189(1):38-51. Epub 2006 Oct 13.

49.

Essential genes of a minimal bacterium.

Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC.

Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):425-30. Epub 2006 Jan 3.

50.

Pyruvate oxidase is a determinant of Avery's rough morphology.

Belanger AE, Clague MJ, Glass JI, Leblanc DJ.

J Bacteriol. 2004 Dec;186(24):8164-71.

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