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Building Cell Structures in Three Dimensions: Electron Tomography Methods for Budding Yeast.

O'Toole ET, Giddings TH Jr, Winey M.

Cold Spring Harb Protoc. 2017 Mar 1;2017(3). doi: 10.1101/pdb.top077685.


Cryopreparation and Electron Tomography of Yeast Cells.

O'Toole ET, Giddings TH Jr, Winey M.

Cold Spring Harb Protoc. 2017 Mar 1;2017(3). doi: 10.1101/pdb.prot085589.


Electron Microscopy of Fission Yeast.

McIntosh JR, Morphew MK, Giddings TH Jr.

Cold Spring Harb Protoc. 2017 Jan 3;2017(1). doi: 10.1101/pdb.top079822.


Cryoelectron Microscopy of Fission Yeast.

Morphew MK, Giddings TH Jr, McIntosh JR.

Cold Spring Harb Protoc. 2017 Jan 3;2017(1). doi: 10.1101/pdb.prot091330.


Immunolocalization of Proteins in Fission Yeast by Electron Microscopy.

Morphew MK, Giddings TH Jr, McIntosh JR.

Cold Spring Harb Protoc. 2017 Jan 3;2017(1). doi: 10.1101/pdb.prot091322.


Preparing Fission Yeast for Electron Microscopy.

Giddings TH Jr, Morphew MK, McIntosh JR.

Cold Spring Harb Protoc. 2017 Jan 3;2017(1). doi: 10.1101/pdb.prot091314.


Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity.

Meehl JB, Bayless BA, Giddings TH Jr, Pearson CG, Winey M.

Mol Biol Cell. 2016 Aug 1;27(15):2394-403. doi: 10.1091/mbc.E16-03-0165. Epub 2016 Jun 1.


The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation.

Fees CP, Aiken J, O'Toole ET, Giddings TH Jr, Moore JK.

Mol Biol Cell. 2016 Jun 1;27(11):1786-96. doi: 10.1091/mbc.E15-05-0300. Epub 2016 Apr 6.


Correction: Katanin Localization Requires Triplet Microtubules in Chlamydomonas reinhardtii.

Esparza JM, O'Toole E, Li L, Giddings TH Jr, Kozak B, Albee AJ, Dutcher SK.

PLoS One. 2016 Jan 15;11(1):e0145569. doi: 10.1371/journal.pone.0145569. eCollection 2016. No abstract available.


A Short CEP135 Splice Isoform Controls Centriole Duplication.

Dahl KD, Sankaran DG, Bayless BA, Pinter ME, Galati DF, Heasley LR, Giddings TH Jr, Pearson CG.

Curr Biol. 2015 Oct 5;25(19):2591-6. doi: 10.1016/j.cub.2015.08.039. Epub 2015 Sep 24.


Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning.

Peng Y, Moritz M, Han X, Giddings TH, Lyon A, Kollman J, Winey M, Yates J 3rd, Agard DA, Drubin DG, Barnes G.

Mol Biol Cell. 2015 Jul 1;26(13):2505-18. doi: 10.1091/mbc.E14-12-1627. Epub 2015 May 13.


DisAp-dependent striated fiber elongation is required to organize ciliary arrays.

Galati DF, Bonney S, Kronenberg Z, Clarissa C, Yandell M, Elde NC, Jerka-Dziadosz M, Giddings TH, Frankel J, Pearson CG.

J Cell Biol. 2014 Dec 22;207(6):705-15. doi: 10.1083/jcb.201409123.


Membrane dynamics at the nuclear exchange junction during early mating (one to four hours) in the ciliate Tetrahymena thermophila.

Cole ES, Giddings TH Jr, Ozzello C, Winey M, O'Toole E, Orias J, Hamilton E, Guerrier S, Ballard A, Aronstein T.

Eukaryot Cell. 2015 Feb;14(2):116-27. doi: 10.1128/EC.00164-14. Epub 2014 Aug 8.


Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast.

Lee IJ, Wang N, Hu W, Schott K, Bähler J, Giddings TH Jr, Pringle JR, Du LL, Wu JQ.

Mol Biol Cell. 2014 Sep 15;25(18):2735-49. doi: 10.1091/mbc.E13-11-0699. Epub 2014 Jul 16.


Conventional transmission electron microscopy.

Winey M, Meehl JB, O'Toole ET, Giddings TH Jr.

Mol Biol Cell. 2014 Feb;25(3):319-23. doi: 10.1091/mbc.E12-12-0863.


ε-tubulin is essential in Tetrahymena thermophila for the assembly and stability of basal bodies.

Ross I, Clarissa C, Giddings TH Jr, Winey M.

J Cell Sci. 2013 Aug 1;126(Pt 15):3441-51. doi: 10.1242/jcs.128694. Epub 2013 May 23.


Katanin localization requires triplet microtubules in Chlamydomonas reinhardtii.

Esparza JM, O'Toole E, Li L, Giddings TH Jr, Kozak B, Albee AJ, Dutcher SK.

PLoS One. 2013;8(1):e53940. doi: 10.1371/journal.pone.0053940. Epub 2013 Jan 8. Erratum in: PLoS One. 2016;11(1):e0145569.


Bld10/Cep135 stabilizes basal bodies to resist cilia-generated forces.

Bayless BA, Giddings TH Jr, Winey M, Pearson CG.

Mol Biol Cell. 2012 Dec;23(24):4820-32. doi: 10.1091/mbc.E12-08-0577. Epub 2012 Oct 31.


The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies.

Vonderfecht T, Cookson MW, Giddings TH Jr, Clarissa C, Winey M.

Mol Biol Cell. 2012 Dec;23(24):4766-77. doi: 10.1091/mbc.E12-06-0454. Epub 2012 Oct 19.


Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure.

O'Toole ET, Giddings TH Jr, Porter ME, Ostrowski LE.

Cytoskeleton (Hoboken). 2012 Aug;69(8):577-90. doi: 10.1002/cm.21035. Epub 2012 May 22.


Cytological analysis of Tetrahymena thermophila.

Winey M, Stemm-Wolf AJ, Giddings TH Jr, Pearson CG.

Methods Cell Biol. 2012;109:357-78. doi: 10.1016/B978-0-12-385967-9.00013-X. Review.


Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii.

Dutcher SK, Li L, Lin H, Meyer L, Giddings TH Jr, Kwan AL, Lewis BL.

G3 (Bethesda). 2012 Jan;2(1):15-22. doi: 10.1534/g3.111.000919. Epub 2012 Jan 1.


The Aurora kinase Ipl1 is necessary for spindle pole body cohesion during budding yeast meiosis.

Shirk K, Jin H, Giddings TH Jr, Winey M, Yu HG.

J Cell Sci. 2011 Sep 1;124(Pt 17):2891-6. doi: 10.1242/jcs.086652.


The two domains of centrin have distinct basal body functions in Tetrahymena.

Vonderfecht T, Stemm-Wolf AJ, Hendershott M, Giddings TH Jr, Meehl JB, Winey M.

Mol Biol Cell. 2011 Jul 1;22(13):2221-34. doi: 10.1091/mbc.E11-02-0151. Epub 2011 May 11.


Deciphering the mode of action of the synthetic antimicrobial peptide Bac8c.

Spindler EC, Hale JD, Giddings TH Jr, Hancock RE, Gill RT.

Antimicrob Agents Chemother. 2011 Apr;55(4):1706-16. doi: 10.1128/AAC.01053-10. Epub 2011 Jan 31.


Electron tomography and immuno-labeling of Tetrahymena thermophila basal bodies.

Giddings TH Jr, Meehl JB, Pearson CG, Winey M.

Methods Cell Biol. 2010;96:117-41. doi: 10.1016/S0091-679X(10)96006-8.


Basal body stability and ciliogenesis requires the conserved component Poc1.

Pearson CG, Osborn DP, Giddings TH Jr, Beales PL, Winey M.

J Cell Biol. 2009 Dec 14;187(6):905-20. doi: 10.1083/jcb.200908019.


High pressure freezing, electron microscopy, and immuno-electron microscopy of Tetrahymena thermophila basal bodies.

Meehl JB, Giddings TH Jr, Winey M.

Methods Mol Biol. 2009;586:227-41. doi: 10.1007/978-1-60761-376-3_12.


Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate Tetrahymena thermophila.

Zweifel E, Smith J, Romero D, Giddings TH Jr, Winey M, Honts J, Dahlseid J, Schneider B, Cole ES.

Eukaryot Cell. 2009 Jun;8(6):899-912. doi: 10.1128/EC.00342-08. Epub 2009 Mar 13.


Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation.

Holinger EP, Old WM, Giddings TH Jr, Wong C, Yates JR 3rd, Winey M.

J Biol Chem. 2009 May 8;284(19):12949-55. doi: 10.1074/jbc.M900088200. Epub 2009 Mar 5.


The two SAS-6 homologs in Tetrahymena thermophila have distinct functions in basal body assembly.

Culver BP, Meehl JB, Giddings TH Jr, Winey M.

Mol Biol Cell. 2009 Mar;20(6):1865-77. doi: 10.1091/mbc.E08-08-0838. Epub 2009 Jan 21.


Basal body components exhibit differential protein dynamics during nascent basal body assembly.

Pearson CG, Giddings TH Jr, Winey M.

Mol Biol Cell. 2009 Feb;20(3):904-14. doi: 10.1091/mbc.E08-08-0835. Epub 2008 Dec 3.


Inactivation of Cdh1 by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle.

Crasta K, Lim HH, Giddings TH Jr, Winey M, Surana U.

Nat Cell Biol. 2008 Jun;10(6):665-75. doi: 10.1038/ncb1729. Epub 2008 May 25.


beta-Catenin is a Nek2 substrate involved in centrosome separation.

Bahmanyar S, Kaplan DD, Deluca JG, Giddings TH Jr, O'Toole ET, Winey M, Salmon ED, Casey PJ, Nelson WJ, Barth AI.

Genes Dev. 2008 Jan 1;22(1):91-105. Epub 2007 Dec 17.


New Tetrahymena basal body protein components identify basal body domain structure.

Kilburn CL, Pearson CG, Romijn EP, Meehl JB, Giddings TH Jr, Culver BP, Yates JR 3rd, Winey M.

J Cell Biol. 2007 Sep 10;178(6):905-12. Epub 2007 Sep 4. Erratum in: J Cell Biol. 2007 Oct 8;179(1):167.


Novel sfi1 alleles uncover additional functions for Sfi1p in bipolar spindle assembly and function.

Anderson VE, Prudden J, Prochnik S, Giddings TH Jr, Hardwick KG.

Mol Biol Cell. 2007 Jun;18(6):2047-56. Epub 2007 Mar 28.


Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography.

O'Toole ET, Giddings TH Jr, Dutcher SK.

Methods Cell Biol. 2007;79:125-43. No abstract available.


The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope.

Jaspersen SL, Martin AE, Glazko G, Giddings TH Jr, Morgan G, Mushegian A, Winey M.

J Cell Biol. 2006 Aug 28;174(5):665-75. Epub 2006 Aug 21.


The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.

Araki Y, Lau CK, Maekawa H, Jaspersen SL, Giddings TH Jr, Schiebel E, Winey M.

Mol Biol Cell. 2006 Apr;17(4):1959-70. Epub 2006 Jan 25.


Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components.

Beliakova-Bethell N, Beckham C, Giddings TH Jr, Winey M, Parker R, Sandmeyer S.

RNA. 2006 Jan;12(1):94-101.


Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family.

Stemm-Wolf AJ, Morgan G, Giddings TH Jr, White EA, Marchione R, McDonald HB, Winey M.

Mol Biol Cell. 2005 Aug;16(8):3606-19. Epub 2005 Jun 8.


Subversion of cellular autophagosomal machinery by RNA viruses.

Jackson WT, Giddings TH Jr, Taylor MP, Mulinyawe S, Rabinovitch M, Kopito RR, Kirkegaard K.

PLoS Biol. 2005 May;3(5):e156. Epub 2005 Apr 26.


The organization of the core proteins of the yeast spindle pole body.

Muller EG, Snydsman BE, Novik I, Hailey DW, Gestaut DR, Niemann CA, O'Toole ET, Giddings TH Jr, Sundin BA, Davis TN.

Mol Biol Cell. 2005 Jul;16(7):3341-52. Epub 2005 May 4.


Three-dimensional ultrastructure of Saccharomyces cerevisiae meiotic spindles.

Winey M, Morgan GP, Straight PD, Giddings TH Jr, Mastronarde DN.

Mol Biol Cell. 2005 Mar;16(3):1178-88. Epub 2005 Jan 5.


Cdc28/Cdk1 regulates spindle pole body duplication through phosphorylation of Spc42 and Mps1.

Jaspersen SL, Huneycutt BJ, Giddings TH Jr, Resing KA, Ahn NG, Winey M.

Dev Cell. 2004 Aug;7(2):263-74.


Requirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae.

Park CJ, Song S, Giddings TH Jr, Ro HS, Sakchaisri K, Park JE, Seong YS, Winey M, Lee KS.

Mol Biol Cell. 2004 Apr;15(4):1711-23. Epub 2004 Feb 6.


Mutations in alpha-tubulin promote basal body maturation and flagellar assembly in the absence of delta-tubulin.

Fromherz S, Giddings TH Jr, Gomez-Ospina N, Dutcher SK.

J Cell Sci. 2004 Jan 15;117(Pt 2):303-14.


Three-dimensional organization of basal bodies from wild-type and delta-tubulin deletion strains of Chlamydomonas reinhardtii.

O'Toole ET, Giddings TH, McIntosh JR, Dutcher SK.

Mol Biol Cell. 2003 Jul;14(7):2999-3012. Epub 2003 Apr 4.

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