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Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2859-E2868. doi: 10.1073/pnas.1721670115. Epub 2018 Mar 5.

hnRNP R and its main interactor, the noncoding RNA 7SK, coregulate the axonal transcriptome of motoneurons.

Author information

1
Institute for Clinical Neurobiology, University of Wuerzburg, 97078 Wuerzburg, Germany.
2
Department of Computer Science, Albert-Ludwigs-Universität Freiburg, 79110 Freiburg, Germany.
3
Centre for Biological Signalling Studies, Albert-Ludwigs-Universität Freiburg, 79104 Freiburg, Germany.
4
Core Unit Systems Medicine, University of Wuerzburg, 97078 Wuerzburg, Germany.
5
Comprehensive Cancer Center Mainfranken, University of Wuerzburg, 97080 Wuerzburg, Germany.
6
Institute for Clinical Neurobiology, University of Wuerzburg, 97078 Wuerzburg, Germany; Sendtner_M@ukw.de.

Abstract

Disturbed RNA processing and subcellular transport contribute to the pathomechanisms of motoneuron diseases such as amyotrophic lateral sclerosis and spinal muscular atrophy. RNA-binding proteins are involved in these processes, but the mechanisms by which they regulate the subcellular diversity of transcriptomes, particularly in axons, are not understood. Heterogeneous nuclear ribonucleoprotein R (hnRNP R) interacts with several proteins involved in motoneuron diseases. It is located in axons of developing motoneurons, and its depletion causes defects in axon growth. Here, we used individual nucleotide-resolution cross-linking and immunoprecipitation (iCLIP) to determine the RNA interactome of hnRNP R in motoneurons. We identified ∼3,500 RNA targets, predominantly with functions in synaptic transmission and axon guidance. Among the RNA targets identified by iCLIP, the noncoding RNA 7SK was the top interactor of hnRNP R. We detected 7SK in the nucleus and also in the cytosol of motoneurons. In axons, 7SK localized in close proximity to hnRNP R, and depletion of hnRNP R reduced axonal 7SK. Furthermore, suppression of 7SK led to defective axon growth that was accompanied by axonal transcriptome alterations similar to those caused by hnRNP R depletion. Using a series of 7SK-deletion mutants, we show that the function of 7SK in axon elongation depends on its interaction with hnRNP R but not with the PTEF-B complex involved in transcriptional regulation. These results propose a role for 7SK as an essential interactor of hnRNP R to regulate its function in axon maintenance.

KEYWORDS:

7SK; axon; hnRNP R; iCLIP; motoneuron

PMID:
29507242
PMCID:
PMC5866599
DOI:
10.1073/pnas.1721670115
[Indexed for MEDLINE]
Free PMC Article

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