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Org Biomol Chem. 2017 Dec 13;15(48):10160-10163. doi: 10.1039/c7ob02032e.

A colorimetric competitive displacement assay for the evaluation of catalytic peptides.

Author information

1
Department of Chemistry, Macalester College, 1600 Grand Ave., Saint Paul, MN 55105, USA. lwitus@macalester.edu.

Abstract

An indicator displacement assay has been adapted to detect the diol products of the aldol reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This provides a rapid colorimetric method of detecting product formation and thus evaluating potential catalysts, which is demonstrated using multiple catalytic peptides.

PMID:
29182189
DOI:
10.1039/c7ob02032e
[Indexed for MEDLINE]

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