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Items: 33

1.

High-specificity single-tube multiplex genotyping using Ribo-PAP PCR, tag primers, alkali cleavage of RNA/DNA chimeras and MALDI-TOF MS.

Mauger F, Gelfand DH, Gupta A, Bodepudi V, Will SG, Bauer K, Myers TW, Gut IG.

Hum Mutat. 2013 Jan;34(1):266-73. doi: 10.1002/humu.22227. Epub 2012 Nov 8.

PMID:
23132774
2.

Ribo-polymerase chain reaction--a facile method for the preparation of chimeric RNA/DNA applied to DNA sequencing.

Mauger F, Bauer K, Semhoun J, Myers TW, Gelfand DH, Gut IG.

Hum Mutat. 2012 Jun;33(6):1010-5. doi: 10.1002/humu.22061. Epub 2012 Apr 4.

PMID:
22354560
3.

DNA-dependent DNA polymerases.

Tabor S, Struhl K, Scharf SJ, Gelfand DH.

Curr Protoc Mol Biol. 2001 May;Chapter 3:Unit3.5. doi: 10.1002/0471142727.mb0305s37.

PMID:
18265231
4.

DNA sequencing by MALDI-TOF MS using alkali cleavage of RNA/DNA chimeras.

Mauger F, Bauer K, Calloway CD, Semhoun J, Nishimoto T, Myers TW, Gelfand DH, Gut IG.

Nucleic Acids Res. 2007;35(8):e62. Epub 2007 Apr 10.

5.

Chimeric thermostable DNA polymerases with reverse transcriptase and attenuated 3'-5' exonuclease activity.

Schönbrunner NJ, Fiss EH, Budker O, Stoffel S, Sigua CL, Gelfand DH, Myers TW.

Biochemistry. 2006 Oct 24;45(42):12786-95.

PMID:
17042497
6.

SNP genotyping using alkali cleavage of RNA/DNA chimeras and MALDI time-of-flight mass spectrometry.

Mauger F, Jaunay O, Chamblain V, Reichert F, Bauer K, Gut IG, Gelfand DH.

Nucleic Acids Res. 2006 Feb 9;34(3):e18.

7.

Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with a Magnesium-Activated Thermostable DNA Polymerase.

Smith ES, Li AK, Wang AM, Gelfand DH, Myers TW.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4115. doi: 10.1101/pdb.prot4115. No abstract available.

PMID:
22485519
8.

Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry.

Sauer S, Gelfand DH, Boussicault F, Bauer K, Reichert F, Gut IG.

Nucleic Acids Res. 2002 Mar 1;30(5):e22.

9.

Transcript quantitation in total yeast cellular RNA using kinetic PCR.

Kang JJ, Watson RM, Fisher ME, Higuchi R, Gelfand DH, Holland MJ.

Nucleic Acids Res. 2000 Jan 15;28(2):e2.

10.

Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymerase.

Auer T, Sninsky JJ, Gelfand DH, Myers TW.

Nucleic Acids Res. 1996 Dec 15;24(24):5021-5.

11.

Cloning, sequencing, and expression of RecA proteins from three distantly related thermophilic eubacteria.

Wetmur JG, Wong DM, Ortiz B, Tong J, Reichert F, Gelfand DH.

J Biol Chem. 1994 Oct 14;269(41):25928-35.

12.
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DNA sequencing with Thermus acquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. 1988.

Innis MS, Myambo KB, Gelfand DH, Brown MA.

Biotechnology. 1992;24:6-10. No abstract available.

PMID:
1422071
14.
15.

Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Holland PM, Abramson RD, Watson R, Gelfand DH.

Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7276-80.

16.

Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase.

Myers TW, Gelfand DH.

Biochemistry. 1991 Aug 6;30(31):7661-6.

PMID:
1714296
17.

Genetic organization of the cellulose synthase operon in Acetobacter xylinum.

Wong HC, Fear AL, Calhoon RD, Eichinger GH, Mayer R, Amikam D, Benziman M, Gelfand DH, Meade JH, Emerick AW, et al.

Proc Natl Acad Sci U S A. 1990 Oct;87(20):8130-4.

18.

Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.

Lawyer FC, Stoffel S, Saiki RK, Myambo K, Drummond R, Gelfand DH.

J Biol Chem. 1989 Apr 15;264(11):6427-37.

19.

DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

Innis MA, Myambo KB, Gelfand DH, Brow MA.

Proc Natl Acad Sci U S A. 1988 Dec;85(24):9436-40.

20.

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA.

Science. 1988 Jan 29;239(4839):487-91.

PMID:
2448875
21.

Expression, Glycosylation, and Secretion of an Aspergillus Glucoamylase by Saccharomyces cerevisiae.

Innis MA, Holland MJ, McCabe PC, Cole GE, Wittman VP, Tal R, Watt KW, Gelfand DH, Holland JP, Meade JH.

Science. 1985 Apr 5;228(4695):21-6.

PMID:
17811549
22.

Analysis of a recessive plasmid copy number mutant: evidence for negative control of Col E1 replication.

Shepard HM, Gelfand DH, Polisky B.

Cell. 1979 Oct;18(2):267-75. No abstract available.

PMID:
387257
23.

Isolation and characterization of ColE1-derived plasmid copy-number mutant.

Gelfand DH, Shepard HM, O'Farrell PH, Polisky B.

Proc Natl Acad Sci U S A. 1978 Dec;75(12):5869-73.

24.

Regulated expression by readthrough translation from a plasmid-encoded beta-galactosidase.

O'Farrell P, Polisky B, Gelfand DH.

J Bacteriol. 1978 May;134(2):645-54.

25.
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28.

A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria.

Polisky B, Bishop RJ, Gelfand DH.

Proc Natl Acad Sci U S A. 1976 Nov;73(11):3900-4.

29.

In vitro synthesis of a DNA dependent RNA polymerase coded on coliphage T7 genome.

Gelfand DH, Hayashi M.

Nature. 1970 Dec 19;228(5277):1162-5. No abstract available.

PMID:
5487241
31.

Initiation of SV40 DNA-directed protein synthesis with N-formylmethionine in vitro.

Bryan RN, Gelfand DH, Hayashi M.

Nature. 1969 Dec 6;224(5223):1019-21. No abstract available.

PMID:
4311804
32.

Electrophoretic characterization of phiX174-specific proteins.

Gelfand DH, Hayashi M.

J Mol Biol. 1969 Sep 28;44(3):501-16. No abstract available.

PMID:
4899475
33.

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