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A micro-volume adaptation of a stopped-flow system; use with μg quantities of muscle proteins.

Walklate J, Ujfalusi Z, Behrens V, King EJ, Geeves MA.

Anal Biochem. 2019 Jun 12. pii: S0003-2697(19)30371-9. doi: 10.1016/j.ab.2019.06.009. [Epub ahead of print]


High-speed imaging of glutamate release with genetically encoded sensors.

Dürst CD, Wiegert JS, Helassa N, Kerruth S, Coates C, Schulze C, Geeves MA, Török K, Oertner TG.

Nat Protoc. 2019 May;14(5):1401-1424. doi: 10.1038/s41596-019-0143-9. Epub 2019 Apr 15.


Nebulin and titin modulate cross-bridge cycling and length-dependent calcium sensitivity.

Mijailovich SM, Stojanovic B, Nedic D, Svicevic M, Geeves MA, Irving TC, Granzier HL.

J Gen Physiol. 2019 May 6;151(5):680-704. doi: 10.1085/jgp.201812165. Epub 2019 Apr 4.


Measurement of Myofilament-Localized Calcium Dynamics in Adult Cardiomyocytes and the Effect of Hypertrophic Cardiomyopathy Mutations.

Sparrow AJ, Sievert K, Patel S, Chang YF, Broyles CN, Brook FA, Watkins H, Geeves MA, Redwood CS, Robinson P, Daniels MJ.

Circ Res. 2019 Apr 12;124(8):1228-1239. doi: 10.1161/CIRCRESAHA.118.314600.


Mg2+ -free ATP regulates the processivity of native cytoplasmic dynein.

Behrens VA, Walter WJ, Peters C, Wang T, Brenner B, Geeves MA, Scholz T, Steffen W.

FEBS Lett. 2019 Feb;593(3):296-307. doi: 10.1002/1873-3468.13319. Epub 2019 Jan 3.


Evidence of functional deficits at the single muscle fiber level in experimentally-induced renal insufficiency.

Mitrou GI, Sakkas GK, Poulianiti KP, Karioti A, Tepetes K, Christodoulidis G, Giakas G, Stefanidis I, Geeves MA, Koutedakis Y, Karatzaferi C.

J Biomech. 2019 Jan 3;82:259-265. doi: 10.1016/j.jbiomech.2018.10.035. Epub 2018 Nov 3.


A novel live-cell imaging system reveals a reversible hydrostatic pressure impact on cell-cycle progression.

Brooker HR, Gyamfi IA, Wieckowska A, Brooks NJ, Mulvihill DP, Geeves MA.

J Cell Sci. 2018 Aug 6;131(15). pii: jcs212167. doi: 10.1242/jcs.212167.


Dilated cardiomyopathy myosin mutants have reduced force-generating capacity.

Ujfalusi Z, Vera CD, Mijailovich SM, Svicevic M, Yu EC, Kawana M, Ruppel KM, Spudich JA, Geeves MA, Leinwand LA.

J Biol Chem. 2018 Jun 8;293(23):9017-9029. doi: 10.1074/jbc.RA118.001938. Epub 2018 Apr 17.


Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer.

Johnson CA, Brooker HR, Gyamfi I, O'Brien J, Ashley B, Brazier JE, Dean A, Embling J, Grimsey E, Tomlinson AC, Wilson EG, Geeves MA, Mulvihill DP.

Biochem Biophys Res Commun. 2018 Nov 25;506(2):339-346. doi: 10.1016/j.bbrc.2017.10.109. Epub 2017 Nov 1.


Modulators of actin-myosin dissociation: basis for muscle type functional differences during fatigue.

Karatzaferi C, Adamek N, Geeves MA.

Am J Physiol Cell Physiol. 2017 Dec 1;313(6):C644-C654. doi: 10.1152/ajpcell.00023.2017. Epub 2017 Sep 20.


More Can Mean Less, or: Simplifying Sometimes Requires Ideas to Be More Complicated.

Geeves MA.

Biophys J. 2017 Jun 20;112(12):2467-2468. doi: 10.1016/j.bpj.2017.05.004. Epub 2017 May 25. No abstract available.


Modeling the Actin.myosin ATPase Cross-Bridge Cycle for Skeletal and Cardiac Muscle Myosin Isoforms.

Mijailovich SM, Nedic D, Svicevic M, Stojanovic B, Walklate J, Ujfalusi Z, Geeves MA.

Biophys J. 2017 Mar 14;112(5):984-996. doi: 10.1016/j.bpj.2017.01.021.


Three-dimensional stochastic model of actin-myosin binding in the sarcomere lattice.

Mijailovich SM, Kayser-Herold O, Stojanovic B, Nedic D, Irving TC, Geeves MA.

J Gen Physiol. 2016 Dec;148(6):459-488. Epub 2016 Nov 18.


Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions.

Brooker HR, Geeves MA, Mulvihill DP.

FEBS Lett. 2016 Sep;590(18):3111-21. doi: 10.1002/1873-3468.12346. Epub 2016 Aug 23.


Review: The ATPase mechanism of myosin and actomyosin.

Geeves MA.

Biopolymers. 2016 Aug;105(8):483-91. doi: 10.1002/bip.22853. Review.


The Most Prevalent Freeman-Sheldon Syndrome Mutations in the Embryonic Myosin Motor Share Functional Defects.

Walklate J, Vera C, Bloemink MJ, Geeves MA, Leinwand L.

J Biol Chem. 2016 May 6;291(19):10318-31. doi: 10.1074/jbc.M115.707489. Epub 2016 Mar 4.


Myosin isoforms and the mechanochemical cross-bridge cycle.

Walklate J, Ujfalusi Z, Geeves MA.

J Exp Biol. 2016 Jan;219(Pt 2):168-74. doi: 10.1242/jeb.124594. Review.


Contractility parameters of human β-cardiac myosin with the hypertrophic cardiomyopathy mutation R403Q show loss of motor function.

Nag S, Sommese RF, Ujfalusi Z, Combs A, Langer S, Sutton S, Leinwand LA, Geeves MA, Ruppel KM, Spudich JA.

Sci Adv. 2015 Oct 9;1(9):e1500511. doi: 10.1126/sciadv.1500511. eCollection 2015 Oct.


The Relay/Converter Interface Influences Hydrolysis of ATP by Skeletal Muscle Myosin II.

Bloemink MJ, Melkani GC, Bernstein SI, Geeves MA.

J Biol Chem. 2016 Jan 22;291(4):1763-73. doi: 10.1074/jbc.M115.688002. Epub 2015 Nov 19.


Phosphorylation of Ser283 enhances the stiffness of the tropomyosin head-to-tail overlap domain.

Lehman W, Medlock G, Li XE, Suphamungmee W, Tu AY, Schmidtmann A, Ujfalusi Z, Fischer S, Moore JR, Geeves MA, Regnier M.

Arch Biochem Biophys. 2015 Apr 1;571:10-5. doi: 10.1016/ Epub 2015 Feb 26.


Temperature manifold for a stopped-flow machine to allow measurements from -10 to +40°C.

Walklate J, Geeves MA.

Anal Biochem. 2015 May 1;476:11-6. doi: 10.1016/j.ab.2015.01.020. Epub 2015 Feb 7.


Using fluorescent myosin to directly visualize cooperative activation of thin filaments.

Desai R, Geeves MA, Kad NM.

J Biol Chem. 2015 Jan 23;290(4):1915-25. doi: 10.1074/jbc.M114.609743. Epub 2014 Nov 26.


A systematic nomenclature for mammalian tropomyosin isoforms.

Geeves MA, Hitchcock-DeGregori SE, Gunning PW.

J Muscle Res Cell Motil. 2015 Apr;36(2):147-53. doi: 10.1007/s10974-014-9389-6. Epub 2014 Nov 5. Review.


Cross-talk, cross-bridges, and calcium activation of cardiac contraction.

Geeves MA, Lehrer SS.

Biophys J. 2014 Aug 5;107(3):543-545. doi: 10.1016/j.bpj.2014.06.019. No abstract available. Erratum in: Biophys J. 2014 Sep 16;107(6):1485.


Use of pyrene-labelled actin to probe actin-myosin interactions: kinetic and equilibrium studies.

Adamek N, Geeves MA.

Exp Suppl. 2014;105:87-104. doi: 10.1007/978-3-0348-0856-9_5. Review.


Rapid reaction kinetic techniques.

Toseland CP, Geeves MA.

Exp Suppl. 2014;105:49-65. doi: 10.1007/978-3-0348-0856-9_3. Review.


FAD binding, cobinamide binding and active site communication in the corrin reductase (CobR).

Lawrence AD, Taylor SL, Scott A, Rowe ML, Johnson CM, Rigby SE, Geeves MA, Pickersgill RW, Howard MJ, Warren MJ.

Biosci Rep. 2014 Jul 4;34(4). pii: e00120. doi: 10.1042/BSR20140060.


The myosin-activated thin filament regulatory state, M⁻-open: a link to hypertrophic cardiomyopathy (HCM).

Lehrer SS, Geeves MA.

J Muscle Res Cell Motil. 2014 Apr;35(2):153-60. doi: 10.1007/s10974-014-9383-z. Epub 2014 Apr 17. Review.


The hypertrophic cardiomyopathy myosin mutation R453C alters ATP binding and hydrolysis of human cardiac β-myosin.

Bloemink M, Deacon J, Langer S, Vera C, Combs A, Leinwand L, Geeves MA.

J Biol Chem. 2014 Feb 21;289(8):5158-67. doi: 10.1074/jbc.M113.511204. Epub 2013 Dec 16.


The superfast human extraocular myosin is kinetically distinct from the fast skeletal IIa, IIb, and IId isoforms.

Bloemink MJ, Deacon JC, Resnicow DI, Leinwand LA, Geeves MA.

J Biol Chem. 2013 Sep 20;288(38):27469-79. doi: 10.1074/jbc.M113.488130. Epub 2013 Aug 1.


Polymorphism in tropomyosin structure and function.

Janco M, Suphamungmee W, Li X, Lehman W, Lehrer SS, Geeves MA.

J Muscle Res Cell Motil. 2013 Aug;34(3-4):177-87. doi: 10.1007/s10974-013-9353-x. Epub 2013 Jul 7. Review.


Production of amino-terminally acetylated recombinant proteins in E. coli.

Johnson M, Geeves MA, Mulvihill DP.

Methods Mol Biol. 2013;981:193-200. doi: 10.1007/978-1-62703-305-3_15.


α-Tropomyosin with a D175N or E180G mutation in only one chain differs from tropomyosin with mutations in both chains.

Janco M, Kalyva A, Scellini B, Piroddi N, Tesi C, Poggesi C, Geeves MA.

Biochemistry. 2012 Dec 11;51(49):9880-90. doi: 10.1021/bi301323n. Epub 2012 Nov 30.


Cooperative regulation of myosin-S1 binding to actin filaments by a continuous flexible Tm-Tn chain.

Mijailovich SM, Kayser-Herold O, Li X, Griffiths H, Geeves MA.

Eur Biophys J. 2012 Dec;41(12):1015-32. doi: 10.1007/s00249-012-0859-8. Epub 2012 Oct 7.


An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis.

Deery E, Schroeder S, Lawrence AD, Taylor SL, Seyedarabi A, Waterman J, Wilson KS, Brown D, Geeves MA, Howard MJ, Pickersgill RW, Warren MJ.

Nat Chem Biol. 2012 Nov;8(11):933-40. doi: 10.1038/nchembio.1086. Epub 2012 Oct 7.


Tuning the calcium sensitivity of cardiac muscle.

Geeves MA, Ranatunga KW.

Biophys J. 2012 Sep 5;103(5):849-50. No abstract available.


Erratum to: Identification of functional differences between recombinant human α and β cardiac myosin motors.

Deacon JC, Bloemink MJ, Rezavandi H, Geeves MA, Leinwand LA.

Cell Mol Life Sci. 2012 Dec;69(24):4239-55. doi: 10.1007/s00018-012-1111-5.


In vitro formation and characterization of the skeletal muscle α·β tropomyosin heterodimers.

Kalyva A, Schmidtmann A, Geeves MA.

Biochemistry. 2012 Aug 14;51(32):6388-99. Epub 2012 Aug 3.


The flexibility of two tropomyosin mutants, D175N and E180G, that cause hypertrophic cardiomyopathy.

Li XE, Suphamungmee W, Janco M, Geeves MA, Marston SB, Fischer S, Lehman W.

Biochem Biophys Res Commun. 2012 Aug 3;424(3):493-6. doi: 10.1016/j.bbrc.2012.06.141. Epub 2012 Jul 9.


Identification of functional differences between recombinant human α and β cardiac myosin motors.

Deacon JC, Bloemink MJ, Rezavandi H, Geeves MA, Leinwand LA.

Cell Mol Life Sci. 2012 Jul;69(13):2261-77. doi: 10.1007/s00018-012-0927-3. Epub 2012 Feb 16. Corrected and republished in: Cell Mol Life Sci. 2012 Dec;69(24):4239-55.


The Hill model for binding myosin S1 to regulated actin is not equivalent to the McKillop-Geeves model.

Mijailovich SM, Li X, Griffiths RH, Geeves MA.

J Mol Biol. 2012 Mar 16;417(1-2):112-28. doi: 10.1016/j.jmb.2012.01.011. Epub 2012 Jan 28.


Shaking the myosin family tree: biochemical kinetics defines four types of myosin motor.

Bloemink MJ, Geeves MA.

Semin Cell Dev Biol. 2011 Dec;22(9):961-7. doi: 10.1016/j.semcdb.2011.09.015. Epub 2011 Oct 4. Review.


Upregulation of cardiomyocyte ribonucleotide reductase increases intracellular 2 deoxy-ATP, contractility, and relaxation.

Korte FS, Dai J, Buckley K, Feest ER, Adamek N, Geeves MA, Murry CE, Regnier M.

J Mol Cell Cardiol. 2011 Dec;51(6):894-901. doi: 10.1016/j.yjmcc.2011.08.026. Epub 2011 Sep 3.


Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V.

Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ.

J Biol Chem. 2011 Oct 7;286(40):35051-60. doi: 10.1074/jbc.M111.265298. Epub 2011 Aug 13.


Dynamics of tropomyosin in muscle fibers as monitored by saturation transfer EPR of bi-functional probe.

Rayes RF, Kálai T, Hideg K, Geeves MA, Fajer PG.

PLoS One. 2011;6(6):e21277. doi: 10.1371/journal.pone.0021277. Epub 2011 Jun 20.


Two Drosophila myosin transducer mutants with distinct cardiomyopathies have divergent ADP and actin affinities.

Bloemink MJ, Melkani GC, Dambacher CM, Bernstein SI, Geeves MA.

J Biol Chem. 2011 Aug 12;286(32):28435-43. doi: 10.1074/jbc.M111.258228. Epub 2011 Jun 16.


Targeted amino-terminal acetylation of recombinant proteins in E. coli.

Johnson M, Coulton AT, Geeves MA, Mulvihill DP.

PLoS One. 2010 Dec 23;5(12):e15801. doi: 10.1371/journal.pone.0015801.


Myo1c mutations associated with hearing loss cause defects in the interaction with nucleotide and actin.

Adamek N, Geeves MA, Coluccio LM.

Cell Mol Life Sci. 2011 Jan;68(1):139-50. doi: 10.1007/s00018-010-0448-x. Epub 2010 Jul 17.


Resolution and uniqueness of estimated parameters of a model of thin filament regulation in solution.

Mijailovich SM, Li X, del Alamo JC, Griffiths RH, Kecman V, Geeves MA.

Comput Biol Chem. 2010 Feb;34(1):19-33. doi: 10.1016/j.compbiolchem.2009.11.002. Epub 2009 Dec 6.


Modification of loop 1 affects the nucleotide binding properties of Myo1c, the adaptation motor in the inner ear.

Adamek N, Lieto-Trivedi A, Geeves MA, Coluccio LM.

Biochemistry. 2010 Feb 9;49(5):958-71. doi: 10.1021/bi901803j.

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