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Items: 38

1.

Biotinylation of Membrane Proteins for Binder Selections.

Kuhn BT, Zimmermann I, Egloff P, Hürlimann LM, Hutter CAJ, Miscenic C, Dawson RJP, Seeger MA, Geertsma ER.

Methods Mol Biol. 2020;2127:151-165. doi: 10.1007/978-1-0716-0373-4_11.

PMID:
32112321
2.

AcrB: a mean, keen, drug efflux machine.

Kobylka J, Kuth MS, Müller RT, Geertsma ER, Pos KM.

Ann N Y Acad Sci. 2020 Jan;1459(1):38-68. doi: 10.1111/nyas.14239. Epub 2019 Oct 6. Review.

PMID:
31588569
3.

Conformation space of a heterodimeric ABC exporter under turnover conditions.

Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Brüchert S, Kuhn BT, Geertsma ER, Hummer G, Tampé R, Moeller A.

Nature. 2019 Jul;571(7766):580-583. doi: 10.1038/s41586-019-1391-0. Epub 2019 Jul 17.

PMID:
31316210
4.

Structural basis for functional interactions in dimers of SLC26 transporters.

Chang YN, Jaumann EA, Reichel K, Hartmann J, Oliver D, Hummer G, Joseph B, Geertsma ER.

Nat Commun. 2019 May 2;10(1):2032. doi: 10.1038/s41467-019-10001-w.

5.

Synthetic single domain antibodies for the conformational trapping of membrane proteins.

Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA.

Elife. 2018 May 24;7. pii: e34317. doi: 10.7554/eLife.34317.

6.

Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers.

Koch K, Kalusche S, Torres JL, Stanfield RL, Danquah W, Khazanehdari K, von Briesen H, Geertsma ER, Wilson IA, Wernery U, Koch-Nolte F, Ward AB, Dietrich U.

Sci Rep. 2017 Aug 21;7(1):8390. doi: 10.1038/s41598-017-08273-7.

7.

Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli.

Marino J, Holzhüter K, Kuhn B, Geertsma ER.

Methods Enzymol. 2017;594:139-164. doi: 10.1016/bs.mie.2017.05.011. Epub 2017 Jul 25.

PMID:
28779839
8.

Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport.

Schenck S, Kunz L, Sahlender D, Pardon E, Geertsma ER, Savtchouk I, Suzuki T, Neldner Y, Štefanić S, Steyaert J, Volterra A, Dutzler R.

Biochemistry. 2017 Aug 1;56(30):3962-3971. doi: 10.1021/acs.biochem.7b00436. Epub 2017 Jul 21.

PMID:
28731329
9.

The novel class of seven transmembrane segment inverted repeat carriers.

Chang YN, Geertsma ER.

Biol Chem. 2017 Feb 1;398(2):165-174. doi: 10.1515/hsz-2016-0254. Review.

PMID:
27865089
10.

Corrigendum: Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family.

Geertsma ER, Chang YN, Shaik FR, Neldner Y, Pardon E, Steyaert J, Dutzler R.

Nat Struct Mol Biol. 2016 May 4;23(5):462. doi: 10.1038/nsmb0516-462. No abstract available.

PMID:
27142325
11.

Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family.

Geertsma ER, Chang YN, Shaik FR, Neldner Y, Pardon E, Steyaert J, Dutzler R.

Nat Struct Mol Biol. 2015 Oct;22(10):803-8. doi: 10.1038/nsmb.3091. Epub 2015 Sep 14. Erratum in: Nat Struct Mol Biol. 2016 May 4;23 (5):462.

PMID:
26367249
12.

A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter.

Bukowska MA, Hohl M, Geertsma ER, Hürlimann LM, Grütter MG, Seeger MA.

Biochemistry. 2015 May 19;54(19):3086-99. doi: 10.1021/acs.biochem.5b00188. Epub 2015 May 7.

PMID:
25947941
13.

Bicistronic mRNAs to enhance membrane protein overexpression.

Marino J, Hohl M, Seeger MA, Zerbe O, Geertsma ER.

J Mol Biol. 2015 Feb 27;427(4):943-954. doi: 10.1016/j.jmb.2014.11.002. Epub 2014 Nov 9.

14.

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.

Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R.

Nat Struct Mol Biol. 2014 Nov;21(11):990-6. doi: 10.1038/nsmb.2904. Epub 2014 Oct 19.

PMID:
25326704
15.

FX cloning: a simple and robust high-throughput cloning method for protein expression.

Geertsma ER.

Methods Mol Biol. 2014;1116:153-64. doi: 10.1007/978-1-62703-764-8_11.

PMID:
24395363
16.

FX cloning: a versatile high-throughput cloning system for characterization of enzyme variants.

Geertsma ER.

Methods Mol Biol. 2013;978:133-48. doi: 10.1007/978-1-62703-293-3_10.

PMID:
23423894
17.

Topogenesis of heterologously expressed fragments of the human Y4 GPCR.

Marino J, Geertsma ER, Zerbe O.

Biochim Biophys Acta. 2012 Dec;1818(12):3055-63. doi: 10.1016/j.bbamem.2012.07.023. Epub 2012 Jul 31.

18.

Mechanism of the prokaryotic transmembrane disulfide reduction pathway and its in vitro reconstitution from purified components.

Malojčić G, Geertsma ER, Brozzo MS, Glockshuber R.

Angew Chem Int Ed Engl. 2012 Jul 9;51(28):6900-3. doi: 10.1002/anie.201201337. Epub 2012 Jun 5.

PMID:
22674494
19.

The response of Lactococcus lactis to membrane protein production.

Marreddy RK, Pinto JP, Wolters JC, Geertsma ER, Fusetti F, Permentier HP, Kuipers OP, Kok J, Poolman B.

PLoS One. 2011;6(8):e24060. doi: 10.1371/journal.pone.0024060. Epub 2011 Aug 31.

20.

A versatile and efficient high-throughput cloning tool for structural biology.

Geertsma ER, Dutzler R.

Biochemistry. 2011 Apr 19;50(15):3272-8. doi: 10.1021/bi200178z. Epub 2011 Mar 25.

PMID:
21410291
21.

Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.

Linares DM, Geertsma ER, Poolman B.

J Mol Biol. 2010 Aug 6;401(1):45-55. doi: 10.1016/j.jmb.2010.06.002. Epub 2010 Jun 11.

PMID:
20542040
22.

Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.

Marreddy RK, Geertsma ER, Permentier HP, Pinto JP, Kok J, Poolman B.

PLoS One. 2010 Apr 26;5(4):e10317. doi: 10.1371/journal.pone.0010317.

23.

Production of membrane proteins in Escherichia coli and Lactococcus lactis.

Geertsma ER, Poolman B.

Methods Mol Biol. 2010;601:17-38. doi: 10.1007/978-1-60761-344-2_2.

PMID:
20099137
24.

A method for site-specific labeling of multiple protein thiols.

Kuiper JM, Pluta R, Huibers WH, Fusetti F, Geertsma ER, Poolman B.

Protein Sci. 2009 May;18(5):1033-41. doi: 10.1002/pro.113.

25.

The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter.

Mulligan C, Geertsma ER, Severi E, Kelly DJ, Poolman B, Thomas GH.

Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1778-83. doi: 10.1073/pnas.0809979106. Epub 2009 Jan 28. Erratum in: Proc Natl Acad Sci U S A. 2009 May 19;106(20):8398.

26.

Quality control of overexpressed membrane proteins.

Geertsma ER, Groeneveld M, Slotboom DJ, Poolman B.

Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5722-7. doi: 10.1073/pnas.0802190105. Epub 2008 Apr 7.

27.

Membrane reconstitution of ABC transporters and assays of translocator function.

Geertsma ER, Nik Mahmood NA, Schuurman-Wolters GK, Poolman B.

Nat Protoc. 2008;3(2):256-66. doi: 10.1038/nprot.2007.519.

PMID:
18274528
28.

High-throughput cloning and expression in recalcitrant bacteria.

Geertsma ER, Poolman B.

Nat Methods. 2007 Sep;4(9):705-7. Epub 2007 Jul 22.

PMID:
17643108
29.

Biochemistry. A missing link in membrane protein evolution.

Poolman B, Geertsma ER, Slotboom DJ.

Science. 2007 Mar 2;315(5816):1229-31. No abstract available.

PMID:
17332400
30.

Flavin binding to the high affinity riboflavin transporter RibU.

Duurkens RH, Tol MB, Geertsma ER, Permentier HP, Slotboom DJ.

J Biol Chem. 2007 Apr 6;282(14):10380-6. Epub 2007 Feb 8.

31.

The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.

Burgess CM, Slotboom DJ, Geertsma ER, Duurkens RH, Poolman B, van Sinderen D.

J Bacteriol. 2006 Apr;188(8):2752-60.

32.

Functional analysis of detergent-solubilized and membrane-reconstituted ATP-binding cassette transporters.

Poolman B, Doeven MK, Geertsma ER, Biemans-Oldehinkel E, Konings WN, Rees DC.

Methods Enzymol. 2005;400:429-59.

PMID:
16399364
33.
34.

Functional interactions between the subunits of the lactose transporter from Streptococcus thermophilus.

Geertsma ER, Duurkens RH, Poolman B.

J Mol Biol. 2005 Jul 1;350(1):102-11.

PMID:
15919090
35.

The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.

Vervoort EB, Bultema JB, Schuurman-Wolters GK, Geertsma ER, Broos J, Poolman B.

J Mol Biol. 2005 Feb 25;346(3):733-43. Epub 2005 Jan 11.

PMID:
15713459
36.

Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles.

Doeven MK, Folgering JH, Krasnikov V, Geertsma ER, van den Bogaart G, Poolman B.

Biophys J. 2005 Feb;88(2):1134-42. Epub 2004 Dec 1.

37.

Identification of the dimer interface of the lactose transport protein from Streptococcus thermophilus.

Geertsma ER, Duurkens RH, Poolman B.

J Mol Biol. 2003 Oct 3;332(5):1165-74.

PMID:
14499618

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