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Items: 1 to 50 of 63

1.

N-succinylamino acid racemases: Enzymatic properties and biotechnological applications.

Martínez-Rodríguez S, Soriano-Maldonado P, Gavira JA.

Biochim Biophys Acta Proteins Proteom. 2020 Apr;1868(4):140377. doi: 10.1016/j.bbapap.2020.140377. Epub 2020 Jan 23. Review.

PMID:
31982578
2.

How Bacterial Chemoreceptors Evolve Novel Ligand Specificities.

Gavira JA, Gumerov VM, Rico-Jiménez M, Petukh M, Upadhyay AA, Ortega A, Matilla MA, Zhulin IB, Krell T.

mBio. 2020 Jan 21;11(1). pii: e03066-19. doi: 10.1128/mBio.03066-19.

3.

Non-conservation of folding rates in the thioredoxin family reveals degradation of ancestral unassisted-folding.

Gamiz-Arco G, Risso VA, Candel AM, Inglés-Prieto A, Romero-Romero ML, Gaucher EA, Gavira JA, Ibarra-Molero B, Sanchez-Ruiz JM.

Biochem J. 2019 Dec 12;476(23):3631-3647. doi: 10.1042/BCJ20190739.

4.

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.

de Wijn R, Hennig O, Roche J, Engilberge S, Rollet K, Fernandez-Millan P, Brillet K, Betat H, Mörl M, Roussel A, Girard E, Mueller-Dieckmann C, Fox GC, Olieric V, Gavira JA, Lorber B, Sauter C.

IUCrJ. 2019 Apr 19;6(Pt 3):454-464. doi: 10.1107/S2052252519003622. eCollection 2019 May 1.

5.

Efficacy of aldose reductase inhibitors is affected by oxidative stress induced under X-ray irradiation.

Castellví A, Crespo I, Crosas E, Cámara-Artigas A, Gavira JA, Aranda MAG, Parés X, Farrés J, Juanhuix J.

Sci Rep. 2019 Feb 28;9(1):3177. doi: 10.1038/s41598-019-39722-0.

6.

The Molecular Mechanism of Nitrate Chemotaxis via Direct Ligand Binding to the PilJ Domain of McpN.

Martín-Mora D, Ortega Á, Matilla MA, Martínez-Rodríguez S, Gavira JA, Krell T.

mBio. 2019 Feb 19;10(1). pii: e02334-18. doi: 10.1128/mBio.02334-18.

7.

Seeding from silica-reinforced lysozyme crystals for neutron crystallography.

Gavira JA, Conejero-Muriel M, Delgado-López JM.

Acta Crystallogr D Struct Biol. 2018 Dec 1;74(Pt 12):1200-1207. doi: 10.1107/S2059798318016054. Epub 2018 Nov 30.

PMID:
30605134
8.

A novel cysteine carbamoyl-switch is responsible for the inhibition of formamidase, a nitrilase superfamily member.

Martínez-Rodríguez S, Conejero-Muriel M, Gavira JA.

Arch Biochem Biophys. 2019 Feb 15;662:151-159. doi: 10.1016/j.abb.2018.12.008. Epub 2018 Dec 5.

PMID:
30528776
9.

Functional Annotation of Bacterial Signal Transduction Systems: Progress and Challenges.

Martín-Mora D, Fernández M, Velando F, Ortega Á, Gavira JA, Matilla MA, Krell T.

Int J Mol Sci. 2018 Nov 26;19(12). pii: E3755. doi: 10.3390/ijms19123755. Review.

10.

High-Affinity Chemotaxis to Histamine Mediated by the TlpQ Chemoreceptor of the Human Pathogen Pseudomonas aeruginosa.

Corral-Lugo A, Matilla MA, Martín-Mora D, Silva Jiménez H, Mesa Torres N, Kato J, Hida A, Oku S, Conejero-Muriel M, Gavira JA, Krell T.

mBio. 2018 Nov 13;9(6). pii: e01894-18. doi: 10.1128/mBio.01894-18.

11.

Structural Basis for Polyamine Binding at the dCACHE Domain of the McpU Chemoreceptor from Pseudomonas putida.

Gavira JA, Ortega Á, Martín-Mora D, Conejero-Muriel MT, Corral-Lugo A, Morel B, Matilla MA, Krell T.

J Mol Biol. 2018 Jun 22;430(13):1950-1963. doi: 10.1016/j.jmb.2018.05.008. Epub 2018 May 11.

PMID:
29758259
12.

Protein separation under a microfluidic regime.

Rodríguez-Ruiz I, Babenko V, Martínez-Rodríguez S, Gavira JA.

Analyst. 2018 Feb 7;143(3):606-619. doi: 10.1039/c7an01568b. Epub 2017 Dec 7.

PMID:
29214270
13.

Enhanced vulnerability of human proteins towards disease-associated inactivation through divergent evolution.

Medina-Carmona E, Fuchs JE, Gavira JA, Mesa-Torres N, Neira JL, Salido E, Palomino-Morales R, Burgos M, Timson DJ, Pey AL.

Hum Mol Genet. 2017 Sep 15;26(18):3531-3544. doi: 10.1093/hmg/ddx238.

PMID:
28911204
14.

De novo active sites for resurrected Precambrian enzymes.

Risso VA, Martinez-Rodriguez S, Candel AM, Krüger DM, Pantoja-Uceda D, Ortega-Muñoz M, Santoyo-Gonzalez F, Gaucher EA, Kamerlin SCL, Bruix M, Gavira JA, Sanchez-Ruiz JM.

Nat Commun. 2017 Jul 18;8:16113. doi: 10.1038/ncomms16113.

15.

Continuous Sensing Photonic Lab-on-a-Chip Platform Based on Cross-Linked Enzyme Crystals.

Conejero-Muriel M, Rodríguez-Ruiz I, Verdugo-Escamilla C, Llobera A, Gavira JA.

Anal Chem. 2016 Dec 6;88(23):11919-11923. Epub 2016 Nov 7.

PMID:
27783896
16.

Identification and characterization of a bacterial hyaluronidase and its production in recombinant form.

Messina L, Gavira JA, Pernagallo S, Unciti-Broceta JD, Sanchez Martin RM, Diaz-Mochon JJ, Vaccaro S, Conejero-Muriel M, Pineda-Molina E, Caruso S, Musumeci L, Di Pasquale R, Pontillo A, Sincinelli F, Pavan M, Secchieri C.

FEBS Lett. 2016 Jul;590(14):2180-9. doi: 10.1002/1873-3468.12258. Epub 2016 Jul 4.

17.

Macromolecular crystallography: An old science with new perspectives.

Cámara-Artigas A, Gavira JA.

Arch Biochem Biophys. 2016 Jul 15;602:1-2. doi: 10.1016/j.abb.2016.05.004. Epub 2016 May 6. No abstract available.

PMID:
27156967
18.

Current trends in protein crystallization.

Gavira JA.

Arch Biochem Biophys. 2016 Jul 15;602:3-11. doi: 10.1016/j.abb.2015.12.010. Epub 2015 Dec 31. Review.

PMID:
26747744
19.

McCLEC, a robust and stable enzymatic based microreactor platform.

Conejero-Muriel M, Rodríguez-Ruiz I, Martínez-Rodríguez S, Llobera A, Gavira JA.

Lab Chip. 2015 Oct 21;15(20):4083-9. doi: 10.1039/c5lc00776c. Epub 2015 Sep 3.

PMID:
26334474
20.

Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis.

Conejero-Muriel M, Gavira JA, Pineda-Molina E, Belsom A, Bradley M, Moral M, García-López Durán Jde D, Luque González A, Díaz-Mochón JJ, Contreras-Montoya R, Martínez-Peragón Á, Cuerva JM, Álvarez de Cienfuegos L.

Chem Commun (Camb). 2015 Mar 4;51(18):3862-5. doi: 10.1039/c4cc09024a.

PMID:
25655841
21.

A multiple path photonic lab on a chip for parallel protein concentration measurements.

Rodríguez-Ruiz I, Conejero-Muriel M, Ackermann TN, Gavira JA, Llobera A.

Lab Chip. 2015 Feb 21;15(4):1133-9. doi: 10.1039/c4lc01332h.

PMID:
25537135
22.

Mutational studies on resurrected ancestral proteins reveal conservation of site-specific amino acid preferences throughout evolutionary history.

Risso VA, Manssour-Triedo F, Delgado-Delgado A, Arco R, Barroso-delJesus A, Ingles-Prieto A, Godoy-Ruiz R, Gavira JA, Gaucher EA, Ibarra-Molero B, Sanchez-Ruiz JM.

Mol Biol Evol. 2015 Feb;32(2):440-55. doi: 10.1093/molbev/msu312. Epub 2014 Nov 12.

23.

Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization.

Gavira JA, Cera-Manjarres A, Ortiz K, Mendez J, Jimenez-Torres JA, Patiño-Lopez LD, Torres-Lugo M.

Cryst Growth Des. 2014 Jul 2;14(7):3239-3248. Epub 2014 May 14.

24.

Cloning, expression, purification, crystallization and preliminary X-ray characterization of allantoinase from Bacillus licheniformis ATCC 14580.

Conejero-Muriel M, Martínez-Gómez AI, Martínez-Rodríguez S, Gavira JA.

Acta Crystallogr F Struct Biol Commun. 2014 Nov;70(Pt 11):1513-6. doi: 10.1107/S2053230X14021608. Epub 2014 Oct 25.

25.

Evolution of conformational dynamics determines the conversion of a promiscuous generalist into a specialist enzyme.

Zou T, Risso VA, Gavira JA, Sanchez-Ruiz JM, Ozkan SB.

Mol Biol Evol. 2015 Jan;32(1):132-43. doi: 10.1093/molbev/msu281. Epub 2014 Oct 13.

PMID:
25312912
26.

Thermostable and promiscuous Precambrian proteins.

Risso VA, Gavira JA, Sanchez-Ruiz JM.

Environ Microbiol. 2014 Jun;16(6):1485-9. No abstract available.

PMID:
25009840
27.

Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins.

Risso VA, Gavira JA, Gaucher EA, Sanchez-Ruiz JM.

Proteins. 2014 Jun;82(6):887-96. doi: 10.1002/prot.24575. Epub 2014 Apr 18.

PMID:
24710963
28.

Introduction to protein crystallization.

McPherson A, Gavira JA.

Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):2-20. doi: 10.1107/S2053230X13033141. Epub 2013 Dec 24. Review.

29.

Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids.

Rico-Jiménez M, Muñoz-Martínez F, Krell T, Gavira JA, Pineda-Molina E.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1431-5. doi: 10.1107/S1744309113023592. Epub 2013 Nov 29.

30.

Conservation of protein structure over four billion years.

Ingles-Prieto A, Ibarra-Molero B, Delgado-Delgado A, Perez-Jimenez R, Fernandez JM, Gaucher EA, Sanchez-Ruiz JM, Gavira JA.

Structure. 2013 Sep 3;21(9):1690-7. doi: 10.1016/j.str.2013.06.020. Epub 2013 Aug 8.

31.

Hyperstability and substrate promiscuity in laboratory resurrections of Precambrian β-lactamases.

Risso VA, Gavira JA, Mejia-Carmona DF, Gaucher EA, Sanchez-Ruiz JM.

J Am Chem Soc. 2013 Feb 27;135(8):2899-902. doi: 10.1021/ja311630a. Epub 2013 Feb 14. Erratum in: J Am Chem Soc. 2013 Jul 17;135(28):10580.

PMID:
23394108
32.

In situ X-ray data collection from highly sensitive crystals of Pseudomonas putida PtxS in complex with DNA.

Pineda-Molina E, Daddaoua A, Krell T, Ramos JL, García-Ruiz JM, Gavira JA.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1307-10. doi: 10.1107/S1744309112028540. Epub 2012 Oct 30.

33.

Evidence for chemoreceptors with bimodular ligand-binding regions harboring two signal-binding sites.

Pineda-Molina E, Reyes-Darias JA, Lacal J, Ramos JL, García-Ruiz JM, Gavira JA, Krell T.

Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18926-31. doi: 10.1073/pnas.1201400109. Epub 2012 Oct 29.

34.

Mutational and structural analysis of L-N-carbamoylase reveals new insights into a peptidase M20/M25/M40 family member.

Martínez-Rodríguez S, García-Pino A, Las Heras-Vázquez FJ, Clemente-Jiménez JM, Rodríguez-Vico F, García-Ruiz JM, Loris R, Gavira JA.

J Bacteriol. 2012 Nov;194(21):5759-68. doi: 10.1128/JB.01056-12. Epub 2012 Aug 17.

35.

Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering.

Oberthuer D, Melero-García E, Dierks K, Meyer A, Betzel C, Garcia-Caballero A, Gavira JA.

PLoS One. 2012;7(6):e33545. doi: 10.1371/journal.pone.0033545. Epub 2012 Jun 4.

36.

Crystallization and crystallographic analysis of the ligand-binding domain of the Pseudomonas putida chemoreceptor McpS in complex with malate and succinate.

Gavira JA, Lacal J, Ramos JL, García-Ruiz JM, Krell T, Pineda-Molina E.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):428-31. doi: 10.1107/S1744309112004940. Epub 2012 Mar 27.

37.

Understanding the polymorphic behaviour of a mutant of the α-spectrin SH3 domain by means of two 1.1 Å resolution structures.

Cámara-Artigas A, Gavira JA, Casares S, Garcia-Ruiz JM, Conejero-Lara F, Allen JP, Martinez JC.

Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):189-96. doi: 10.1107/S0907444911001715. Epub 2011 Feb 15.

PMID:
21358049
38.

Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.

Stsiapanava A, Dohnalek J, Gavira JA, Kuty M, Koudelakova T, Damborsky J, Kuta Smatanova I.

Acta Crystallogr D Biol Crystallogr. 2010 Sep;66(Pt 9):962-9. doi: 10.1107/S0907444910027101. Epub 2010 Aug 13.

PMID:
20823547
39.

Biophysical and atomic force microscopy characterization of the RNA from satellite tobacco mosaic virus.

Kuznetsov YG, Dowell JJ, Gavira JA, Ng JD, McPherson A.

Nucleic Acids Res. 2010 Dec;38(22):8284-94. doi: 10.1093/nar/gkq662. Epub 2010 Aug 6.

40.

Novel conformational aspects of the third PDZ domain of the neuronal post-synaptic density-95 protein revealed from two 1.4A X-ray structures.

Cámara-Artigas A, Murciano-Calles J, Gavira JA, Cobos ES, Martínez JC.

J Struct Biol. 2010 Jun;170(3):565-9. doi: 10.1016/j.jsb.2010.03.005. Epub 2010 Mar 19.

PMID:
20227506
41.

Two-step counterdiffusion protocol for the crystallization of haemoglobin II from Lucina pectinata in the pH range 4-9.

Nieves-Marrero CA, Ruiz-Martínez CR, Estremera-Andújar RA, González-Ramírez LA, López-Garriga J, Gavira JA.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):264-8. doi: 10.1107/S1744309109053081. Epub 2010 Feb 24.

42.

Modulation of buried ionizable groups in proteins with engineered surface charge.

Pey AL, Rodriguez-Larrea D, Gavira JA, Garcia-Moreno B, Sanchez-Ruiz JM.

J Am Chem Soc. 2010 Feb 3;132(4):1218-9. doi: 10.1021/ja909298v.

PMID:
20055447
43.

Counterdiffusion methods applied to protein crystallization.

Otálora F, Gavira JA, Ng JD, García-Ruiz JM.

Prog Biophys Mol Biol. 2009 Nov;101(1-3):26-37. doi: 10.1016/j.pbiomolbio.2009.12.004. Epub 2009 Dec 16. Review.

PMID:
20018206
44.

Structure of dihydropyrimidinase from Sinorhizobium meliloti CECT4114: new features in an amidohydrolase family member.

Martínez-Rodríguez S, Martínez-Gómez AI, Clemente-Jiménez JM, Rodríguez-Vico F, García-Ruíz JM, Las Heras-Vázquez FJ, Gavira JA.

J Struct Biol. 2010 Feb;169(2):200-8. doi: 10.1016/j.jsb.2009.10.013. Epub 2009 Nov 4.

PMID:
19895890
45.

Crystallization and diffraction patterns of the oxy and cyano forms of the Lucina pectinata haemoglobins complex.

Ruiz-Martínez CR, Nieves-Marrero CA, Estremera-Andújar RA, Gavira JA, González-Ramírez LA, López-Garriga J, García-Ruiz JM.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jan 1;65(Pt 1):25-8. doi: 10.1107/S1744309108038542. Epub 2008 Dec 25.

46.

Crystallization and preliminary crystallographic studies of the recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43.

Martínez-Rodríguez S, García-Pino A, Las Heras-Vázquez FJ, Clemente-Jiménez JM, Rodríguez-Vico F, Loris R, García-Ruiz JM, Gavira JA.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt 12):1135-8. doi: 10.1107/S1744309108034830. Epub 2008 Nov 28.

47.

Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis.

Perez-Jimenez R, Wiita AP, Rodriguez-Larrea D, Kosuri P, Gavira JA, Sanchez-Ruiz JM, Fernandez JM.

J Biol Chem. 2008 Oct 3;283(40):27121-9. doi: 10.1074/jbc.M803746200. Epub 2008 Aug 7.

48.

Structure and ligand selection of hemoglobin II from Lucina pectinata.

Gavira JA, Camara-Artigas A, De Jesús-Bonilla W, López-Garriga J, Lewis A, Pietri R, Yeh SR, Cadilla CL, García-Ruiz JM.

J Biol Chem. 2008 Apr 4;283(14):9414-23. doi: 10.1074/jbc.M705026200. Epub 2008 Jan 18.

49.

Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.

Martínez-Rodríguez S, González-Ramírez LA, Clemente-Jiménez JM, Rodríguez-Vico F, Las Heras-Vázquez FJ, Gavira JA, García-Ruiz JM.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):50-3. Epub 2007 Dec 20.

50.

Crystallization by capillary counter-diffusion and structure determination of the N114A mutant of the SH3 domain of Abl tyrosine kinase complexed with a high-affinity peptide ligand.

Cámara-Artigas A, Palencia A, Martínez JC, Luque I, Gavira JA, García-Ruiz JM.

Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):646-52. Epub 2007 Apr 21.

PMID:
17452790

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