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Items: 1 to 50 of 148

1.

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy.

Becke TD, Ness S, Sudhop S, Gaub HE, Hilleringmann M, Schilling AF, Clausen-Schaumann H.

J Vis Exp. 2018 Aug 20;(138). doi: 10.3791/58167.

PMID:
30176022
2.

Enzyme-Mediated, Site-Specific Protein Coupling Strategies for Surface-Based Binding Assays.

Ott W, Durner E, Gaub HE.

Angew Chem Int Ed Engl. 2018 Sep 24;57(39):12666-12669. doi: 10.1002/anie.201805034. Epub 2018 Aug 31.

PMID:
30080959
3.

Post-Translational Sortase-Mediated Attachment of High-Strength Force Spectroscopy Handles.

Durner E, Ott W, Nash MA, Gaub HE.

ACS Omega. 2017 Jun 30;2(6):3064-3069. doi: 10.1021/acsomega.7b00478.

4.

Ligand Binding Stabilizes Cellulosomal Cohesins as Revealed by AFM-based Single-Molecule Force Spectroscopy.

Verdorfer T, Gaub HE.

Sci Rep. 2018 Jun 25;8(1):9634. doi: 10.1038/s41598-018-27085-x.

5.

Molecular mechanism of extreme mechanostability in a pathogen adhesin.

Milles LF, Schulten K, Gaub HE, Bernardi RC.

Science. 2018 Mar 30;359(6383):1527-1533. doi: 10.1126/science.aar2094.

PMID:
29599244
6.

Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry.

Sedlak SM, Bauer MS, Kluger C, Schendel LC, Milles LF, Pippig DA, Gaub HE.

PLoS One. 2017 Dec 5;12(12):e0188722. doi: 10.1371/journal.pone.0188722. eCollection 2017.

7.

Combining in Vitro and in Silico Single-Molecule Force Spectroscopy to Characterize and Tune Cellulosomal Scaffoldin Mechanics.

Verdorfer T, Bernardi RC, Meinhold A, Ott W, Luthey-Schulten Z, Nash MA, Gaub HE.

J Am Chem Soc. 2017 Dec 13;139(49):17841-17852. doi: 10.1021/jacs.7b07574. Epub 2017 Nov 3.

8.

Increasing evidence of mechanical force as a functional regulator in smooth muscle myosin light chain kinase.

Baumann F, Bauer MS, Rees M, Alexandrovich A, Gautel M, Pippig DA, Gaub HE.

Elife. 2017 Jul 11;6. pii: e26473. doi: 10.7554/eLife.26473.

9.

Elastin-like Polypeptide Linkers for Single-Molecule Force Spectroscopy.

Ott W, Jobst MA, Bauer MS, Durner E, Milles LF, Nash MA, Gaub HE.

ACS Nano. 2017 Jun 27;11(6):6346-6354. doi: 10.1021/acsnano.7b02694. Epub 2017 Jun 12.

PMID:
28591514
10.

Membrane proteins scrambling through a folding landscape.

Müller DJ, Gaub HE.

Science. 2017 Mar 3;355(6328):907-908. doi: 10.1126/science.aam8370. No abstract available.

PMID:
28254899
11.

Mechanical Stability of a High-Affinity Toxin Anchor from the Pathogen Clostridium perfringens.

Milles LF, Bayer EA, Nash MA, Gaub HE.

J Phys Chem B. 2017 Apr 20;121(15):3620-3625. doi: 10.1021/acs.jpcb.6b09593. Epub 2016 Dec 19.

PMID:
27991799
12.

Biasing effects of receptor-ligand complexes on protein-unfolding statistics.

Schoeler C, Verdorfer T, Gaub HE, Nash MA.

Phys Rev E. 2016 Oct;94(4-1):042412. Epub 2016 Oct 13.

PMID:
27841541
13.

Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation.

Ott W, Nicolaus T, Gaub HE, Nash MA.

Biomacromolecules. 2016 Apr 11;17(4):1330-8. doi: 10.1021/acs.biomac.5b01726. Epub 2016 Mar 22.

PMID:
26974874
14.

Single-molecule force spectroscopy on polyproteins and receptor-ligand complexes: The current toolbox.

Ott W, Jobst MA, Schoeler C, Gaub HE, Nash MA.

J Struct Biol. 2017 Jan;197(1):3-12. doi: 10.1016/j.jsb.2016.02.011. Epub 2016 Feb 9. Review.

PMID:
26873782
15.

Resolving dual binding conformations of cellulosome cohesin-dockerin complexes using single-molecule force spectroscopy.

Jobst MA, Milles LF, Schoeler C, Ott W, Fried DB, Bayer EA, Gaub HE, Nash MA.

Elife. 2015 Oct 31;4. pii: e10319. doi: 10.7554/eLife.10319.

16.

Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy.

Baumann F, Bauer MS, Milles LF, Alexandrovich A, Gaub HE, Pippig DA.

Nat Nanotechnol. 2016 Jan;11(1):89-94. doi: 10.1038/nnano.2015.231. Epub 2015 Oct 12.

PMID:
26457965
17.

Energy profile of nanobody-GFP complex under force.

Klamecka K, Severin PM, Milles LF, Gaub HE, Leonhardt H.

Phys Biol. 2015 Sep 10;12(5):056009. doi: 10.1088/1478-3975/12/5/056009.

PMID:
26356046
18.

Mapping Mechanical Force Propagation through Biomolecular Complexes.

Schoeler C, Bernardi RC, Malinowska KH, Durner E, Ott W, Bayer EA, Schulten K, Nash MA, Gaub HE.

Nano Lett. 2015 Nov 11;15(11):7370-6. doi: 10.1021/acs.nanolett.5b02727. Epub 2015 Aug 19.

19.

Quantifying Synergy, Thermostability, and Targeting of Cellulolytic Enzymes and Cellulosomes with Polymerization-Based Amplification.

Malinowska KH, Rind T, Verdorfer T, Gaub HE, Nash MA.

Anal Chem. 2015 Jul 21;87(14):7133-40. doi: 10.1021/acs.analchem.5b00936. Epub 2015 Jun 26.

PMID:
26114625
20.

C-5 Propynyl Modifications Enhance the Mechanical Stability of DNA.

Aschenbrenner D, Baumann F, Milles LF, Pippig DA, Gaub HE.

Chemphyschem. 2015 Jul 20;16(10):2085-90. doi: 10.1002/cphc.201500193. Epub 2015 May 15.

PMID:
25982589
21.

Tip localization of an atomic force microscope in transmission microscopy with nanoscale precision.

Baumann F, Heucke SF, Pippig DA, Gaub HE.

Rev Sci Instrum. 2015 Mar;86(3):035109. doi: 10.1063/1.4915145.

PMID:
25832277
22.

Parallel force assay for protein-protein interactions.

Aschenbrenner D, Pippig DA, Klamecka K, Limmer K, Leonhardt H, Gaub HE.

PLoS One. 2014 Dec 29;9(12):e115049. doi: 10.1371/journal.pone.0115049. eCollection 2014. Erratum in: PLoS One. 2015;10(3):e0119695.

23.

Ultrastable cellulosome-adhesion complex tightens under load.

Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA.

Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635.

24.

Redox-initiated hydrogel system for detection and real-time imaging of cellulolytic enzyme activity.

Malinowska KH, Verdorfer T, Meinhold A, Milles LF, Funk V, Gaub HE, Nash MA.

ChemSusChem. 2014 Oct;7(10):2759. doi: 10.1002/cssc.201402796. Epub 2014 Sep 11. No abstract available.

PMID:
25213832
25.

From genes to protein mechanics on a chip.

Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash MA, Gaub HE.

Nat Methods. 2014 Nov;11(11):1127-1130. doi: 10.1038/nmeth.3099. Epub 2014 Sep 7.

26.

Single molecule techniques--applications in biology.

Puchner EM, Huang B, Gaub HE.

FEBS Lett. 2014 Oct 1;588(19):3519. doi: 10.1016/j.febslet.2014.08.020. Epub 2014 Aug 23. No abstract available.

27.

Functionalization of cantilever tips with nucleotides by the phosphoramidite method.

David R, Erdmann M, Fornof AR, Gaub HE.

ChemMedChem. 2014 Sep;9(9):2049-51. doi: 10.1002/cmdc.201402165. Epub 2014 Aug 11.

PMID:
25130700
28.

Redox-initiated hydrogel system for detection and real-time imaging of cellulolytic enzyme activity.

Malinowska KH, Verdorfer T, Meinhold A, Milles LF, Funk V, Gaub HE, Nash MA.

ChemSusChem. 2014 Oct;7(10):2825-31. doi: 10.1002/cssc.201402428. Epub 2014 Aug 12.

PMID:
25116339
29.

Protein-DNA chimeras for nano assembly.

Pippig DA, Baumann F, Strackharn M, Aschenbrenner D, Gaub HE.

ACS Nano. 2014 Jul 22;8(7):6551-5. doi: 10.1021/nn501644w. Epub 2014 Jun 6.

PMID:
24897163
30.

A force-based, parallel assay for the quantification of protein-DNA interactions.

Limmer K, Pippig DA, Aschenbrenner D, Gaub HE.

PLoS One. 2014 Feb 27;9(2):e89626. doi: 10.1371/journal.pone.0089626. eCollection 2014.

31.

Stamping vital cells - a force-based ligand receptor assay.

Wienken U, Gaub HE.

Biophys J. 2013 Dec 17;105(12):2687-94. doi: 10.1016/j.bpj.2013.10.013.

32.

Protein-DNA force assay in a microfluidic format.

Otten M, Wolf P, Gaub HE.

Lab Chip. 2013 Nov 7;13(21):4198-204. doi: 10.1039/c3lc50830g.

PMID:
23986395
33.

Placing individual molecules in the center of nanoapertures.

Heucke SF, Baumann F, Acuna GP, Severin PM, Stahl SW, Strackharn M, Stein IH, Altpeter P, Tinnefeld P, Gaub HE.

Nano Lett. 2014 Feb 12;14(2):391-5. doi: 10.1021/nl401517a. Epub 2013 Jun 17.

PMID:
23742166
34.

Effects of cytosine hydroxymethylation on DNA strand separation.

Severin PM, Zou X, Schulten K, Gaub HE.

Biophys J. 2013 Jan 8;104(1):208-15. doi: 10.1016/j.bpj.2012.11.013. Epub 2013 Jan 8.

35.

Sequence-specific inhibition of Dicer measured with a force-based microarray for RNA ligands.

Limmer K, Aschenbrenner D, Gaub HE.

Nucleic Acids Res. 2013 Apr 1;41(6):e69. doi: 10.1093/nar/gks1455. Epub 2013 Jan 8.

36.

Single-molecule dissection of the high-affinity cohesin-dockerin complex.

Stahl SW, Nash MA, Fried DB, Slutzki M, Barak Y, Bayer EA, Gaub HE.

Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20431-6. doi: 10.1073/pnas.1211929109. Epub 2012 Nov 27.

37.

Single-molecule adhesion of a stimuli-responsive oligo(ethylene glycol) copolymer to gold.

Nash MA, Gaub HE.

ACS Nano. 2012 Dec 21;6(12):10735-42. doi: 10.1021/nn303963m. Epub 2012 Nov 6.

PMID:
23113802
38.

The state of nanoparticle-based nanoscience and biotechnology: progress, promises, and challenges.

Pelaz B, Jaber S, de Aberasturi DJ, Wulf V, Aida T, de la Fuente JM, Feldmann J, Gaub HE, Josephson L, Kagan CR, Kotov NA, Liz-Marzán LM, Mattoussi H, Mulvaney P, Murray CB, Rogach AL, Weiss PS, Willner I, Parak WJ.

ACS Nano. 2012 Oct 23;6(10):8468-83. doi: 10.1021/nn303929a. Epub 2012 Sep 27.

PMID:
23016700
39.

Nanoscale arrangement of proteins by single-molecule cut-and-paste.

Strackharn M, Pippig DA, Meyer P, Stahl SW, Gaub HE.

J Am Chem Soc. 2012 Sep 19;134(37):15193-6. Epub 2012 Sep 7.

PMID:
22950442
40.

DNA-protein binding force chip.

Severin PM, Gaub HE.

Small. 2012 Nov 5;8(21):3269-73. doi: 10.1002/smll.201201088. Epub 2012 Aug 9. No abstract available.

PMID:
22887737
41.

Single-molecule mechanoenzymatics.

Puchner EM, Gaub HE.

Annu Rev Biophys. 2012;41:497-518. doi: 10.1146/annurev-biophys-050511-102301. Review.

PMID:
22577826
42.

Functional assembly of aptamer binding sites by single-molecule cut-and-paste.

Strackharn M, Stahl SW, Puchner EM, Gaub HE.

Nano Lett. 2012 May 9;12(5):2425-8. doi: 10.1021/nl300422y. Epub 2012 Apr 6.

PMID:
22468898
43.

Peptide-antibody complex as handle for single-molecule cut & paste.

Strackharn M, Stahl SW, Severin PM, Nicolaus T, Gaub HE.

Chemphyschem. 2012 Mar;13(4):914-7. doi: 10.1002/cphc.201100765. Epub 2011 Dec 19.

PMID:
22184012
44.

A conditional gating mechanism assures the integrity of the molecular force-sensor titin kinase.

Stahl SW, Puchner EM, Alexandrovich A, Gautel M, Gaub HE.

Biophys J. 2011 Oct 19;101(8):1978-86. doi: 10.1016/j.bpj.2011.09.027.

45.

Cytosine methylation alters DNA mechanical properties.

Severin PM, Zou X, Gaub HE, Schulten K.

Nucleic Acids Res. 2011 Nov 1;39(20):8740-51. doi: 10.1093/nar/gkr578. Epub 2011 Jul 20.

46.

Electric glue: electrically controlled polymer-surface adhesion.

Fornof AR, Erdmann M, David R, Gaub HE.

Nano Lett. 2011 May 11;11(5):1993-6. doi: 10.1021/nl200353h. Epub 2011 Apr 26.

PMID:
21517123
47.

Atomic force microscope-based single-molecule force spectroscopy of RNA unfolding.

Heus HA, Puchner EM, van Vugt-Jonker AJ, Zimmermann JL, Gaub HE.

Anal Biochem. 2011 Jul 1;414(1):1-6. doi: 10.1016/j.ab.2011.03.012. Epub 2011 Mar 12.

PMID:
21402049
48.

Five challenges to bringing single-molecule force spectroscopy into living cells.

Dufrêne YF, Evans E, Engel A, Helenius J, Gaub HE, Müller DJ.

Nat Methods. 2011 Feb;8(2):123-7. doi: 10.1038/nmeth0211-123.

PMID:
21278722
49.

A high throughput molecular force assay for protein-DNA interactions.

Severin PM, Ho D, Gaub HE.

Lab Chip. 2011 Mar 7;11(5):856-62. doi: 10.1039/c0lc00302f. Epub 2011 Jan 10.

PMID:
21221429
50.

Electrically induced bonding of DNA to gold.

Erdmann M, David R, Fornof AR, Gaub HE.

Nat Chem. 2010 Sep;2(9):745-9. doi: 10.1038/nchem.722. Epub 2010 Jul 4.

PMID:
20729894

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