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Items: 24


Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.

Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB.

Mol Cell. 2019 Jul 11;75(1):90-101.e5. doi: 10.1016/j.molcel.2019.04.020. Epub 2019 May 9.


Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic Cyanobacterium Aphanizomenon flos-aquae Reveals Novel Insights into Virus-Bacterium Interactions.

Šulčius S, Šimoliūnas E, Alzbutas G, Gasiūnas G, Jauniškis V, Kuznecova J, Miettinen S, Nilsson E, Meškys R, Roine E, Paškauskas R, Holmfeldt K.

Appl Environ Microbiol. 2018 Dec 13;85(1). pii: e01311-18. doi: 10.1128/AEM.01311-18. Print 2019 Jan 1.


A closely-related clade of globally distributed bloom-forming cyanobacteria within the Nostocales.

Driscoll CB, Meyer KA, Šulčius S, Brown NM, Dick GJ, Cao H, Gasiūnas G, Timinskas A, Yin Y, Landry ZC, Otten TG, Davis TW, Watson SB, Dreher TW.

Harmful Algae. 2018 Jul;77:93-107. doi: 10.1016/j.hal.2018.05.009. Epub 2018 Jun 29.


DnaQ exonuclease-like domain of Cas2 promotes spacer integration in a type I-E CRISPR-Cas system.

Drabavicius G, Sinkunas T, Silanskas A, Gasiunas G, Venclovas Č, Siksnys V.

EMBO Rep. 2018 Jul;19(7). pii: e45543. doi: 10.15252/embr.201745543. Epub 2018 Jun 11.


Harnessing the natural diversity and in vitro evolution of Cas9 to expand the genome editing toolbox.

Karvelis T, Gasiunas G, Siksnys V.

Curr Opin Microbiol. 2017 Jun;37:88-94. doi: 10.1016/j.mib.2017.05.009. Epub 2017 Jun 20. Review.


Methods for decoding Cas9 protospacer adjacent motif (PAM) sequences: A brief overview.

Karvelis T, Gasiunas G, Siksnys V.

Methods. 2017 May 15;121-122:3-8. doi: 10.1016/j.ymeth.2017.03.006. Epub 2017 Mar 24. Review.


Design of a CRISPR-Cas system to increase resistance of Bacillus subtilis to bacteriophage SPP1.

Jakutyte-Giraitiene L, Gasiunas G.

J Ind Microbiol Biotechnol. 2016 Aug;43(8):1183-8. doi: 10.1007/s10295-016-1784-0. Epub 2016 Jun 2.


Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging.

Labrie V, Buske OJ, Oh E, Jeremian R, Ptak C, Gasiūnas G, Maleckas A, Petereit R, Žvirbliene A, Adamonis K, Kriukienė E, Koncevičius K, Gordevičius J, Nair A, Zhang A, Ebrahimi S, Oh G, Šikšnys V, Kupčinskas L, Brudno M, Petronis A.

Nat Struct Mol Biol. 2016 Jun;23(6):566-73. doi: 10.1038/nsmb.3227. Epub 2016 May 9.


Rewiring Cas9 to Target New PAM Sequences.

Siksnys V, Gasiunas G.

Mol Cell. 2016 Mar 17;61(6):793-4. doi: 10.1016/j.molcel.2016.03.002.


Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome.

Müller M, Lee CM, Gasiunas G, Davis TH, Cradick TJ, Siksnys V, Bao G, Cathomen T, Mussolino C.

Mol Ther. 2016 Mar;24(3):636-44. doi: 10.1038/mt.2015.218. Epub 2015 Dec 14.


Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements.

Karvelis T, Gasiunas G, Young J, Bigelyte G, Silanskas A, Cigan M, Siksnys V.

Genome Biol. 2015 Nov 19;16:253. doi: 10.1186/s13059-015-0818-7.


Cas3 nuclease-helicase activity assays.

Sinkunas T, Gasiunas G, Siksnys V.

Methods Mol Biol. 2015;1311:277-91. doi: 10.1007/978-1-4939-2687-9_18.


Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex.

Glemzaite M, Balciunaite E, Karvelis T, Gasiunas G, Grusyte MM, Alzbutas G, Jurcyte A, Anderson EM, Maksimova E, Smith AJ, Lubys A, Zaliauskiene L, Siksnys V.

RNA Biol. 2015;12(1):1-4. doi: 10.1080/15476286.2015.1017209. No abstract available.


Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

Szczelkun MD, Tikhomirova MS, Sinkunas T, Gasiunas G, Karvelis T, Pschera P, Siksnys V, Seidel R.

Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9798-803. doi: 10.1073/pnas.1402597111. Epub 2014 May 27.


Programmable DNA cleavage in vitro by Cas9.

Karvelis T, Gasiunas G, Siksnys V.

Biochem Soc Trans. 2013 Dec;41(6):1401-6. doi: 10.1042/BST20130164. Review.


RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?

Gasiunas G, Siksnys V.

Trends Microbiol. 2013 Nov;21(11):562-7. doi: 10.1016/j.tim.2013.09.001. Epub 2013 Oct 1.


Molecular mechanisms of CRISPR-mediated microbial immunity.

Gasiunas G, Sinkunas T, Siksnys V.

Cell Mol Life Sci. 2014 Feb;71(3):449-65. Review.


crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus.

Karvelis T, Gasiunas G, Miksys A, Barrangou R, Horvath P, Siksnys V.

RNA Biol. 2013 May;10(5):841-51. doi: 10.4161/rna.24203. Epub 2013 Mar 27.


In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus.

Sinkunas T, Gasiunas G, Waghmare SP, Dickman MJ, Barrangou R, Horvath P, Siksnys V.

EMBO J. 2013 Feb 6;32(3):385-94. doi: 10.1038/emboj.2012.352. Epub 2013 Jan 18.


Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria.

Gasiunas G, Barrangou R, Horvath P, Siksnys V.

Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):E2579-86. Epub 2012 Sep 4.


The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli.

Sapranauskas R, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V.

Nucleic Acids Res. 2011 Nov;39(21):9275-82. doi: 10.1093/nar/gkr606. Epub 2011 Aug 3.


Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system.

Sinkunas T, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V.

EMBO J. 2011 Apr 6;30(7):1335-42. doi: 10.1038/emboj.2011.41. Epub 2011 Feb 22.


Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family.

Gasiunas G, Sasnauskas G, Tamulaitis G, Urbanke C, Razaniene D, Siksnys V.

Nucleic Acids Res. 2008 Feb;36(3):938-49. Epub 2007 Dec 17.


Mva1269I: a monomeric type IIS restriction endonuclease from Micrococcus varians with two EcoRI- and FokI-like catalytic domains.

Armalyte E, Bujnicki JM, Giedriene J, Gasiunas G, Kosiński J, Lubys A.

J Biol Chem. 2005 Dec 16;280(50):41584-94. Epub 2005 Oct 11.

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