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PLoS One. 2018 Dec 12;13(12):e0207881. doi: 10.1371/journal.pone.0207881. eCollection 2018.

Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples.

Author information

LAI, Aix Marseille Univ, CNRS, INSERM, Marseille, France.
CINAM, Aix Marseille Univ, CNRS, Marseille, France.
Univ Mons, Res Inst Biosci, Interfaces & Complex Fluids Lab, Mechanobiol & Soft Matter, Mons, Belgium.
LCB, Aix Marseille Univ, CNRS, Marseille, France.
CRPP, Univ Bordeaux, CNRS, Pessac, France.
Nanolane, Le Mans, France.


Macroscopic properties of physical and biological processes like friction, wetting, and adhesion or cell migration are controlled by interfacial properties at the nanoscopic scale. In an attempt to bridge simultaneously investigations at different scales, we demonstrate here how optical microscopy in Wet-Surface Ellipsometric Enhanced Contrast (Wet-SEEC) mode offers imaging and measurement of thin films at solid/liquid interfaces in the range 1-500 nm with lateral optical resolution. A live, label-free and noninvasive methodology integrated with microfluidic devices allowed here characterization of polymers and proteins patterns together with corresponding phenotypes of living cells.

Conflict of interest statement

We have the following interests: This work was supported by the Nanolane company (Le Mans), Solvay company (Bordeaux), Alveole company (Paris). MM was employed by Nanolane. There are no patents, products in development or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.

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