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The Rel stringent factor from Thermus thermophilus: crystallization and X-ray analysis.

Van Nerom K, Tamman H, Takada H, Hauryliuk V, Garcia-Pino A.

Acta Crystallogr F Struct Biol Commun. 2019 Aug 1;75(Pt 8):561-569. doi: 10.1107/S2053230X19010628. Epub 2019 Aug 2.


Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.

Sigoillot M, Overtus M, Grodecka M, Scholl D, Garcia-Pino A, Laeremans T, He L, Pardon E, Hildebrandt E, Urbatsch I, Steyaert J, Riordan JR, Govaerts C.

Nat Commun. 2019 Jun 14;10(1):2636. doi: 10.1038/s41467-019-10714-y.


A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.

Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R.

Nat Commun. 2019 Feb 27;10(1):972. doi: 10.1038/s41467-019-08865-z.


Mechanism of regulation and neutralization of the AtaR-AtaT toxin-antitoxin system.

Jurėnas D, Van Melderen L, Garcia-Pino A.

Nat Chem Biol. 2019 Mar;15(3):285-294. doi: 10.1038/s41589-018-0216-z. Epub 2019 Feb 4.


Regulation of (p)ppGpp hydrolysis by a conserved archetypal regulatory domain.

Ronneau S, Caballero-Montes J, Coppine J, Mayard A, Garcia-Pino A, Hallez R.

Nucleic Acids Res. 2019 Jan 25;47(2):843-854. doi: 10.1093/nar/gky1201.


Reply to Holden and Errington, "Type II Toxin-Antitoxin Systems and Persister Cells".

Goormaghtigh F, Fraikin N, Putrinš M, Hauryliuk V, Garcia-Pino A, Udekwu K, Tenson T, Kaldalu N, Van Melderen L.

MBio. 2018 Sep 25;9(5). pii: e01838-18. doi: 10.1128/mBio.01838-18. No abstract available.


A molecular mechanism for Wnt ligand-specific signaling.

Eubelen M, Bostaille N, Cabochette P, Gauquier A, Tebabi P, Dumitru AC, Koehler M, Gut P, Alsteens D, Stainier DYR, Garcia-Pino A, Vanhollebeke B.

Science. 2018 Aug 17;361(6403). pii: eaat1178. doi: 10.1126/science.aat1178. Epub 2018 Jul 19.


Crystallization and X-ray analysis of all of the players in the autoregulation of the ataRT toxin-antitoxin system.

Jurėnas D, Van Melderen L, Garcia-Pino A.

Acta Crystallogr F Struct Biol Commun. 2018 Jul 1;74(Pt 7):391-401. doi: 10.1107/S2053230X18007914. Epub 2018 Jun 26.


Reassessing the Role of Type II Toxin-Antitoxin Systems in Formation of Escherichia coli Type II Persister Cells.

Goormaghtigh F, Fraikin N, Putrinš M, Hallaert T, Hauryliuk V, Garcia-Pino A, Sjödin A, Kasvandik S, Udekwu K, Tenson T, Kaldalu N, Van Melderen L.

MBio. 2018 Jun 12;9(3). pii: e00640-18. doi: 10.1128/mBio.00640-18.


ARE-mediated decay controls gene expression and cellular metabolism upon oxygen variations.

de Toeuf B, Soin R, Nazih A, Dragojevic M, Jurėnas D, Delacourt N, Vo Ngoc L, Garcia-Pino A, Kruys V, Gueydan C.

Sci Rep. 2018 Mar 26;8(1):5211. doi: 10.1038/s41598-018-23551-8.


Phosphorylation decelerates conformational dynamics in bacterial translation elongation factors.

Talavera A, Hendrix J, Versées W, Jurėnas D, Van Nerom K, Vandenberk N, Singh RK, Konijnenberg A, De Gieter S, Castro-Roa D, Barth A, De Greve H, Sobott F, Hofkens J, Zenkin N, Loris R, Garcia-Pino A.

Sci Adv. 2018 Mar 14;4(3):eaap9714. doi: 10.1126/sciadv.aap9714. eCollection 2018 Mar.


Messing up translation from the start: How AtaT inhibits translation initiation in E. coli.

Van Melderen L, Jurenas D, Garcia-Pino A.

RNA Biol. 2018 Mar 4;15(3):303-307. doi: 10.1080/15476286.2017.1391439. Epub 2018 Jan 30.


Novel toxins from type II toxin-antitoxin systems with acetyltransferase activity.

Jurėnas D, Garcia-Pino A, Van Melderen L.

Plasmid. 2017 Sep;93:30-35. doi: 10.1016/j.plasmid.2017.08.005. Epub 2017 Sep 20. Review.


Production, biophysical characterization and crystallization of Pseudomonas putida GraA and its complexes with GraT and the graTA operator.

Talavera A, Tamman H, Ainelo A, Hadǽi S, Garcia-Pino A, Hõrak R, Konijnenberg A, Loris R.

Acta Crystallogr F Struct Biol Commun. 2017 Aug 1;73(Pt 8):455-462. doi: 10.1107/S2053230X17009438. Epub 2017 Jul 26.


AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet.

Jurėnas D, Chatterjee S, Konijnenberg A, Sobott F, Droogmans L, Garcia-Pino A, Van Melderen L.

Nat Chem Biol. 2017 Jun;13(6):640-646. doi: 10.1038/nchembio.2346. Epub 2017 Apr 3.


Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a β-strand sliding mechanism.

Hadži S, Garcia-Pino A, Haesaerts S, Jurenas D, Gerdes K, Lah J, Loris R.

Nucleic Acids Res. 2017 May 5;45(8):4972-4983. doi: 10.1093/nar/gkx138.


Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain.

Huculeci R, Kieken F, Garcia-Pino A, Buts L, van Nuland N, Lenaerts T.

Methods Mol Biol. 2017;1555:257-267. doi: 10.1007/978-1-4939-6762-9_14.


Frizzled 7 and PIP2 binding by syntenin PDZ2 domain supports Frizzled 7 trafficking and signalling.

Egea-Jimenez AL, Gallardo R, Garcia-Pino A, Ivarsson Y, Wawrzyniak AM, Kashyap R, Loris R, Schymkowitz J, Rousseau F, Zimmermann P.

Nat Commun. 2016 Jul 8;7:12101. doi: 10.1038/ncomms12101.


An intrinsically disordered entropic switch determines allostery in Phd-Doc regulation.

Garcia-Pino A, De Gieter S, Talavera A, De Greve H, Efremov RG, Loris R.

Nat Chem Biol. 2016 Jul;12(7):490-6. doi: 10.1038/nchembio.2078. Epub 2016 May 2.


Substrate Recognition and Activity Regulation of the Escherichia coli mRNA Endonuclease MazF.

Zorzini V, Mernik A, Lah J, Sterckx YG, De Jonge N, Garcia-Pino A, De Greve H, Versées W, Loris R.

J Biol Chem. 2016 May 20;291(21):10950-60. doi: 10.1074/jbc.M116.715912. Epub 2016 Mar 29.


A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex.

Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R.

J Mol Biol. 2016 Apr 24;428(8):1589-603. doi: 10.1016/j.jmb.2016.03.007. Epub 2016 Mar 18.


Structural insights into the intertwined dimer of fyn SH2.

Huculeci R, Garcia-Pino A, Buts L, Lenaerts T, van Nuland N.

Protein Sci. 2015 Dec;24(12):1964-78. doi: 10.1002/pro.2806. Epub 2015 Oct 7.


Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module.

Hadzi S, Garcia-Pino A, Gerdes K, Lah J, Loris R.

Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):226-33. doi: 10.1107/S2053230X15000746. Epub 2015 Jan 28.


An efficient method for the purification of proteins from four distinct toxin-antitoxin modules.

Sterckx YG, De Gieter S, Zorzini V, Hadži S, Haesaerts S, Loris R, Garcia-Pino A.

Protein Expr Purif. 2015 Apr;108:30-40. doi: 10.1016/j.pep.2015.01.001. Epub 2015 Jan 9.


The intrinsically disordered domain of the antitoxin Phd chaperones the toxin Doc against irreversible inactivation and misfolding.

De Gieter S, Konijnenberg A, Talavera A, Butterer A, Haesaerts S, De Greve H, Sobott F, Loris R, Garcia-Pino A.

J Biol Chem. 2014 Dec 5;289(49):34013-23. doi: 10.1074/jbc.M114.572396. Epub 2014 Oct 16.


A nanobody modulates the p53 transcriptional program without perturbing its functional architecture.

Bethuyne J, De Gieter S, Zwaenepoel O, Garcia-Pino A, Durinck K, Verhelle A, Hassanzadeh-Ghassabeh G, Speleman F, Loris R, Gettemans J.

Nucleic Acids Res. 2014 Nov 10;42(20):12928-38. doi: 10.1093/nar/gku962. Epub 2014 Oct 16.


Disorder- and dynamics-based regulatory mechanisms in toxin-antitoxin modules.

Loris R, Garcia-Pino A.

Chem Rev. 2014 Jul 9;114(13):6933-47. doi: 10.1021/cr400656f. Epub 2014 May 7. Review. No abstract available.


Small-angle X-ray scattering- and nuclear magnetic resonance-derived conformational ensemble of the highly flexible antitoxin PaaA2.

Sterckx YG, Volkov AN, Vranken WF, Kragelj J, Jensen MR, Buts L, Garcia-Pino A, Jové T, Van Melderen L, Blackledge M, van Nuland NA, Loris R.

Structure. 2014 Jun 10;22(6):854-65. doi: 10.1016/j.str.2014.03.012. Epub 2014 Apr 24.


Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics.

Zorzini V, Buts L, Sleutel M, Garcia-Pino A, Talavera A, Haesaerts S, De Greve H, Cheung A, van Nuland NA, Loris R.

Nucleic Acids Res. 2014 Jun;42(10):6709-25. doi: 10.1093/nar/gku266. Epub 2014 Apr 19.


SAXS analysis of the tRNA-modifying enzyme complex MnmE/MnmG reveals a novel interaction mode and GTP-induced oligomerization.

Fislage M, Brosens E, Deyaert E, Spilotros A, Pardon E, Loris R, Steyaert J, Garcia-Pino A, Versées W.

Nucleic Acids Res. 2014 May;42(9):5978-92. doi: 10.1093/nar/gku213. Epub 2014 Mar 14.


The many faces of Fic: structural and functional aspects of Fic enzymes.

Garcia-Pino A, Zenkin N, Loris R.

Trends Biochem Sci. 2014 Mar;39(3):121-9. doi: 10.1016/j.tibs.2014.01.001. Epub 2014 Feb 5. Review.


Study of the structural and dynamic effects in the FimH adhesin upon α-d-heptyl mannose binding.

Vanwetswinkel S, Volkov AN, Sterckx YG, Garcia-Pino A, Buts L, Vranken WF, Bouckaert J, Roy R, Wyns L, van Nuland NA.

J Med Chem. 2014 Feb 27;57(4):1416-27. doi: 10.1021/jm401666c. Epub 2014 Feb 7.


The structure of TAX1BP1 UBZ1+2 provides insight into target specificity and adaptability.

Ceregido MA, Spínola Amilibia M, Buts L, Rivera-Torres J, Garcia-Pino A, Bravo J, van Nuland NA.

J Mol Biol. 2014 Feb 6;426(3):674-90. doi: 10.1016/j.jmb.2013.11.006. Epub 2013 Nov 13.


The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu.

Castro-Roa D, Garcia-Pino A, De Gieter S, van Nuland NAJ, Loris R, Zenkin N.

Nat Chem Biol. 2013 Dec;9(12):811-7. doi: 10.1038/nchembio.1364. Epub 2013 Oct 20.


Crystallization of the HigBA2 toxin-antitoxin complex from Vibrio cholerae.

Hadži S, Garcia-Pino A, Martinez-Rodriguez S, Verschueren K, Christensen-Dalsgaard M, Gerdes K, Lah J, Loris R.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):1052-9. doi: 10.1107/S1744309113021490. Epub 2013 Aug 27.


Multimeric and differential binding of CIN85/CD2AP with two atypical proline-rich sequences from CD2 and Cbl-b*.

Ceregido MA, Garcia-Pino A, Ortega-Roldan JL, Casares S, López Mayorga O, Bravo J, van Nuland NA, Azuaga AI.

FEBS J. 2013 Jul;280(14):3399-415. doi: 10.1111/febs.12333. Epub 2013 Jun 10.


Structural determinants for activity and specificity of the bacterial toxin LlpA.

Ghequire MG, Garcia-Pino A, Lebbe EK, Spaepen S, Loris R, De Mot R.

PLoS Pathog. 2013 Feb;9(2):e1003199. doi: 10.1371/journal.ppat.1003199. Epub 2013 Feb 28.


¹H, ¹³C, and ¹⁵N backbone and side-chain chemical shift assignment of the toxin Doc in the unbound state.

De Gieter S, Loris R, van Nuland NA, Garcia-Pino A.

Biomol NMR Assign. 2014 Apr;8(1):145-8. doi: 10.1007/s12104-013-9471-9. Epub 2013 Feb 19.


Mutational and structural analysis of L-N-carbamoylase reveals new insights into a peptidase M20/M25/M40 family member.

Martínez-Rodríguez S, García-Pino A, Las Heras-Vázquez FJ, Clemente-Jiménez JM, Rodríguez-Vico F, García-Ruiz JM, Loris R, Gavira JA.

J Bacteriol. 2012 Nov;194(21):5759-68. doi: 10.1128/JB.01056-12. Epub 2012 Aug 17.


SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly.

Baranova E, Fronzes R, Garcia-Pino A, Van Gerven N, Papapostolou D, Péhau-Arnaudet G, Pardon E, Steyaert J, Howorka S, Remaut H.

Nature. 2012 Jul 5;487(7405):119-22. doi: 10.1038/nature11155.


The ParE2-PaaA2 toxin-antitoxin complex from Escherichia coli O157 forms a heterodocecamer in solution and in the crystal.

Sterckx YG, Garcia-Pino A, Haesaerts S, Jové T, Geerts L, Sakellaris V, Van Melderen L, Loris R.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):724-9. doi: 10.1107/S1744309112015230. Epub 2012 May 25.


Alternative interactions define gyrase specificity in the CcdB family.

De Jonge N, Simic M, Buts L, Haesaerts S, Roelants K, Garcia-Pino A, Sterckx Y, De Greve H, Lah J, Loris R.

Mol Microbiol. 2012 Jun;84(5):965-78. doi: 10.1111/j.1365-2958.2012.08069.x. Epub 2012 May 14.


Purification, crystallization and preliminary X-ray diffraction analysis of the Fyn SH2 domain and its complex with a phosphotyrosine peptide.

Huculeci R, Buts L, Lenaerts T, van Nuland NA, Garcia-Pino A.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar 1;68(Pt 3):359-64. doi: 10.1107/S1744309112004186. Epub 2012 Feb 29.


The plasticity of the β-trefoil fold constitutes an evolutionary platform for protease inhibition.

Azarkan M, Martinez-Rodriguez S, Buts L, Baeyens-Volant D, Garcia-Pino A.

J Biol Chem. 2011 Dec 23;286(51):43726-34. doi: 10.1074/jbc.M111.291310. Epub 2011 Oct 25.


Allostery and intrinsic disorder mediate transcription regulation by conditional cooperativity.

Garcia-Pino A, Balasubramanian S, Wyns L, Gazit E, De Greve H, Magnuson RD, Charlier D, van Nuland NA, Loris R.

Cell. 2010 Jul 9;142(1):101-11. doi: 10.1016/j.cell.2010.05.039.


Purification and crystallization of Phd, the antitoxin of the phd/doc operon.

Garcia-Pino A, Sterckx Y, Vandenbussche G, Loris R.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):167-71. doi: 10.1107/S1744309109051550. Epub 2010 Jan 27.


Structural and thermodynamic characterization of Vibrio fischeri CcdB.

De Jonge N, Hohlweg W, Garcia-Pino A, Respondek M, Buts L, Haesaerts S, Lah J, Zangger K, Loris R.

J Biol Chem. 2010 Feb 19;285(8):5606-13. doi: 10.1074/jbc.M109.068429. Epub 2009 Dec 2.


Structural and thermodynamic characterization of pre- and postpolymerization states in the F4 fimbrial subunit FaeG.

Van Molle I, Moonens K, Garcia-Pino A, Buts L, De Kerpel M, Wyns L, Bouckaert J, De Greve H.

J Mol Biol. 2009 Dec 18;394(5):957-67. doi: 10.1016/j.jmb.2009.09.059. Epub 2009 Sep 30.


Rejuvenation of CcdB-poisoned gyrase by an intrinsically disordered protein domain.

De Jonge N, Garcia-Pino A, Buts L, Haesaerts S, Charlier D, Zangger K, Wyns L, De Greve H, Loris R.

Mol Cell. 2009 Jul 31;35(2):154-63. doi: 10.1016/j.molcel.2009.05.025.


The F4 fimbrial chaperone FaeE is stable as a monomer that does not require self-capping of its pilin-interactive surfaces.

Van Molle I, Moonens K, Buts L, Garcia-Pino A, Panjikar S, Wyns L, De Greve H, Bouckaert J.

Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):411-20. doi: 10.1107/S0907444909005174. Epub 2009 Apr 18.


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