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Items: 45

1.

Complete Draft Genome Sequence of Escherichia coli K802.

Kleiner-Grote GRM, Wibberg D, Winkler A, Kalinowski J, Friehs K.

Genome Announc. 2017 Sep 28;5(39). pii: e00934-17. doi: 10.1128/genomeA.00934-17.

2.

Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase.

Schwarzhans JP, Wibberg D, Winkler A, Kalinowski J, Friehs K.

Genome Announc. 2017 Sep 28;5(39). pii: e00933-17. doi: 10.1128/genomeA.00933-17.

3.

Towards systems metabolic engineering in Pichia pastoris.

Schwarzhans JP, Luttermann T, Geier M, Kalinowski J, Friehs K.

Biotechnol Adv. 2017 Nov 1;35(6):681-710. doi: 10.1016/j.biotechadv.2017.07.009. Epub 2017 Jul 29. Review.

PMID:
28760369
4.

A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production.

Schwarzhans JP, Luttermann T, Wibberg D, Winkler A, Hübner W, Huser T, Kalinowski J, Friehs K.

Front Microbiol. 2017 May 2;8:780. doi: 10.3389/fmicb.2017.00780. eCollection 2017.

5.

Fermentative production of L-pipecolic acid from glucose and alternative carbon sources.

Pérez-García F, Max Risse J, Friehs K, Wendisch VF.

Biotechnol J. 2017 Jul;12(7). doi: 10.1002/biot.201600646. Epub 2017 Mar 9.

PMID:
28169491
6.

Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing.

Schwarzhans JP, Wibberg D, Winkler A, Luttermann T, Kalinowski J, Friehs K.

Sci Rep. 2016 Dec 13;6:38952. doi: 10.1038/srep38952.

7.

Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.

Schwarzhans JP, Wibberg D, Winkler A, Luttermann T, Kalinowski J, Friehs K.

Microb Cell Fact. 2016 May 20;15:84. doi: 10.1186/s12934-016-0486-7.

8.

Complete Draft Genome Sequence of Escherichia coli JF733.

Kleiner GR, Wibberg D, Winkler A, Kalinowski J, Wertz JE, Friehs K.

Genome Announc. 2016 Apr 21;4(2). pii: e00298-16. doi: 10.1128/genomeA.00298-16.

9.

GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris.

Müller JM, Bruhn S, Flaschel E, Friehs K, Risse JM.

Biotechnol Prog. 2016 Jul 8;32(4):855-64. doi: 10.1002/btpr.2283. Epub 2016 May 4.

PMID:
27090387
10.

Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development.

Wetzel D, Müller JM, Flaschel E, Friehs K, Risse JM.

J Biotechnol. 2016 May 10;225:3-9. doi: 10.1016/j.jbiotec.2016.03.017. Epub 2016 Mar 14.

PMID:
26988393
11.

Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant'.

Müller JM, Wetzel D, Flaschel E, Friehs K, Risse JM.

J Biotechnol. 2016 Mar 10;221:91-100. doi: 10.1016/j.jbiotec.2016.01.032. Epub 2016 Jan 25.

PMID:
26820322
12.

Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion.

Grimm P, Risse JM, Cholewa D, Müller JM, Beshay U, Friehs K, Flaschel E.

J Biotechnol. 2015 Dec 10;215:72-9. doi: 10.1016/j.jbiotec.2015.04.004. Epub 2015 Apr 21.

PMID:
25910451
13.

Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA).

Velur Selvamani RS, Telaar M, Friehs K, Flaschel E.

Microb Cell Fact. 2014 Apr 21;13:58. doi: 10.1186/1475-2859-13-58.

14.

Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism.

Velur Selvamani RS, Friehs K, Flaschel E.

Bioprocess Biosyst Eng. 2014 Mar;37(3):401-13. doi: 10.1007/s00449-013-1005-4. Epub 2013 Jul 3.

PMID:
23820825
15.

Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins.

Sommer B, Friehs K, Flaschel E.

J Biotechnol. 2010 Feb 15;145(4):350-8. doi: 10.1016/j.jbiotec.2009.11.019. Epub 2009 Dec 1.

PMID:
19958803
16.

Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein.

Sommer B, Friehs K, Flaschel E, Reck M, Stahl F, Scheper T.

J Biotechnol. 2009 Mar 25;140(3-4):194-202. doi: 10.1016/j.jbiotec.2009.01.010. Epub 2009 Jan 29.

PMID:
19428714
17.

A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification.

Wei N, Flaschel E, Friehs K, Nattkemper TW.

BMC Bioinformatics. 2008 Oct 21;9:449. doi: 10.1186/1471-2105-9-449.

18.

Systematic investigation of optimal aptamer immobilization for protein-microarray applications.

Walter JG, Kökpinar O, Friehs K, Stahl F, Scheper T.

Anal Chem. 2008 Oct 1;80(19):7372-8. doi: 10.1021/ac801081v. Epub 2008 Aug 27.

PMID:
18729475
19.

Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli.

Beshay U, Miksch G, Friehs K, Flaschel E.

Bioprocess Biosyst Eng. 2009 Feb;32(2):149-58. doi: 10.1007/s00449-008-0227-3. Epub 2008 May 15. Erratum in: Bioprocess Biosyst Eng. 2009 Feb;32(2):159.

PMID:
18481103
20.

Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling.

Vijayendran C, Barsch A, Friehs K, Niehaus K, Becker A, Flaschel E.

Genome Biol. 2008 Apr 10;9(4):R72. doi: 10.1186/gb-2008-9-4-r72.

21.

2DBase: 2D-PAGE database of Escherichia coli.

Vijayendran C, Burgemeister S, Friehs K, Niehaus K, Flaschel E.

Biochem Biophys Res Commun. 2007 Nov 23;363(3):822-7. Epub 2007 Sep 21.

PMID:
17904107
22.

Recycling of bacterial biomass in a process of L-threonine production by means of a recombinant strain of Escherichia coli.

Blaesen M, Friehs K, Flaschel E.

J Biotechnol. 2007 Dec 1;132(4):431-7. Epub 2007 Aug 10.

PMID:
17868941
23.

Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter.

Beshay U, Miksch G, Friehs K, Flaschel E.

Biotechnol Lett. 2007 Dec;29(12):1893-901. Epub 2007 Jul 25.

PMID:
17653622
24.

The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12.

Vijayendran C, Polen T, Wendisch VF, Friehs K, Niehaus K, Flaschel E.

J Biotechnol. 2007 Mar 10;128(4):747-61. Epub 2007 Jan 14.

PMID:
17331609
25.
26.

In situ dark field microscopy for on-line monitoring of yeast cultures.

Wei N, You J, Friehs K, Flaschel E, Nattkemper TW.

Biotechnol Lett. 2007 Mar;29(3):373-8. Epub 2006 Dec 21.

PMID:
17186133
27.

The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli.

Miksch G, Bettenworth F, Friehs K, Flaschel E.

Appl Microbiol Biotechnol. 2005 Dec;69(3):312-20. Epub 2005 Nov 15.

PMID:
16088348
28.

Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli.

Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Twellmann T, Nattkemper TW.

J Biotechnol. 2005 Oct 17;120(1):25-37. Epub 2005 Jul 12.

PMID:
16019099
29.

A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli.

Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Nattkemper TW.

Appl Microbiol Biotechnol. 2006 Mar;70(2):229-36. Epub 2005 Jul 13.

PMID:
16012833
30.

Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand.

Lu Y, Knol JC, Linskens MH, Friehs K, van Haastert PJ, Flaschel E.

Appl Microbiol Biotechnol. 2004 Oct;65(5):547-52. Epub 2004 May 14.

PMID:
15146296
31.

Plasmid copy number and plasmid stability.

Friehs K.

Adv Biochem Eng Biotechnol. 2004;86:47-82. Review.

PMID:
15088763
32.

Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium.

Lu Y, Knol JC, Linskens MH, Friehs K, Van Haastert PJ, Flaschel E.

J Biotechnol. 2004 Mar 18;108(3):243-51.

PMID:
15006425
33.

Production of supercoiled multimeric plasmid DNA for biopharmaceutical application.

Voss C, Schmidt T, Schleef M, Friehs K, Flaschel E.

J Biotechnol. 2003 Nov 6;105(3):205-13.

PMID:
14580792
34.

Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation.

Beshay U, Friehs K, Azzam AE, Flaschel E.

Bioprocess Biosyst Eng. 2003 Dec;26(2):117-22. Epub 2003 Oct 18.

PMID:
14566554
35.

Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies.

Kleist S, Miksch G, Hitzmann B, Arndt M, Friehs K, Flaschel E.

Appl Microbiol Biotechnol. 2003 Jun;61(5-6):456-62. Epub 2003 Mar 18.

PMID:
12764560
36.

Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors.

Miksch G, Kleist S, Friehs K, Flaschel E.

Appl Microbiol Biotechnol. 2002 Sep;59(6):685-94. Epub 2002 Jul 17.

PMID:
12226725
37.

Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis.

Schmidt T, Friehs K, Schleef M, Voss C, Flaschel E.

Anal Biochem. 1999 Oct 15;274(2):235-40.

PMID:
10527521
38.

High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium.

Miksch G, Neitzel R, Friehs K, Flaschel E.

Appl Microbiol Biotechnol. 1999 May;51(5):627-32.

PMID:
10390821
39.

The green fluorescent protein is a versatile reporter for bioprocess monitoring.

Poppenborg L, Friehs K, Flaschel E.

J Biotechnol. 1997 Oct 17;58(2):79-88.

PMID:
9383982
41.
42.

Rapid determination of plasmid copy number.

Schmidt T, Friehs K, Flaschel E.

J Biotechnol. 1996 Aug 20;49(1-3):219-29.

PMID:
8879172
43.
44.

Parameters influencing the productivity of recombinant E. coli cultivations.

Friehs K, Reardon KF.

Adv Biochem Eng Biotechnol. 1993;48:53-77. Review.

PMID:
8460577
45.

Strategies for improving plasmid stability in genetically modified bacteria in bioreactors.

Kumar PK, Maschke HE, Friehs K, Schügerl K.

Trends Biotechnol. 1991 Aug;9(8):279-84. Review.

PMID:
1367568

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