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Items: 1 to 50 of 72

1.

Combinatorial impact of Sec signal peptides from Bacillus subtilis and bioprocess conditions on heterologous cutinase secretion by Corynebacterium glutamicum.

Hemmerich J, Moch M, Jurischka S, Wiechert W, Freudl R, Oldiges M.

Biotechnol Bioeng. 2019 Mar;116(3):644-655. doi: 10.1002/bit.26873. Epub 2018 Dec 31.

PMID:
30450544
2.

Signal peptides for recombinant protein secretion in bacterial expression systems.

Freudl R.

Microb Cell Fact. 2018 Mar 29;17(1):52. doi: 10.1186/s12934-018-0901-3. Review.

3.

The early mature part of bacterial twin-arginine translocation (Tat) precursor proteins contributes to TatBC receptor binding.

Ulfig A, Freudl R.

J Biol Chem. 2018 May 11;293(19):7281-7299. doi: 10.1074/jbc.RA118.002576. Epub 2018 Mar 28.

4.

The three-component system EsrISR regulates a cell envelope stress response in Corynebacterium glutamicum.

Kleine B, Chattopadhyay A, Polen T, Pinto D, Mascher T, Bott M, Brocker M, Freudl R.

Mol Microbiol. 2017 Dec;106(5):719-741. doi: 10.1111/mmi.13839. Epub 2017 Oct 12.

5.

How to achieve Tat transport with alien TatA.

Hauer RS, Freudl R, Dittmar J, Jakob M, Klösgen RB.

Sci Rep. 2017 Aug 18;7(1):8808. doi: 10.1038/s41598-017-08818-w.

6.

The h-region of twin-arginine signal peptides supports productive binding of bacterial Tat precursor proteins to the TatBC receptor complex.

Ulfig A, Fröbel J, Lausberg F, Blümmel AS, Heide AK, Müller M, Freudl R.

J Biol Chem. 2017 Jun 30;292(26):10865-10882. doi: 10.1074/jbc.M117.788950. Epub 2017 May 17.

7.

Beyond amino acids: Use of the Corynebacterium glutamicum cell factory for the secretion of heterologous proteins.

Freudl R.

J Biotechnol. 2017 Sep 20;258:101-109. doi: 10.1016/j.jbiotec.2017.02.023. Epub 2017 Feb 24.

PMID:
28238807
8.

Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum.

Hemmerich J, Rohe P, Kleine B, Jurischka S, Wiechert W, Freudl R, Oldiges M.

Microb Cell Fact. 2016 Dec 7;15(1):208.

9.

A TatABC-type Tat translocase is required for unimpaired aerobic growth of Corynebacterium glutamicum ATCC13032.

Oertel D, Schmitz S, Freudl R.

PLoS One. 2015 Apr 2;10(4):e0123413. doi: 10.1371/journal.pone.0123413. eCollection 2015.

10.

Leaving home ain't easy: protein export systems in Gram-positive bacteria.

Freudl R.

Res Microbiol. 2013 Jul-Aug;164(6):664-74. doi: 10.1016/j.resmic.2013.03.014. Epub 2013 Mar 26. Review.

PMID:
23541477
11.

Transmembrane insertion of twin-arginine signal peptides is driven by TatC and regulated by TatB.

Fröbel J, Rose P, Lausberg F, Blümmel AS, Freudl R, Müller M.

Nat Commun. 2012;3:1311. doi: 10.1038/ncomms2308.

12.

Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol-xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum.

Scheele S, Oertel D, Bongaerts J, Evers S, Hellmuth H, Maurer KH, Bott M, Freudl R.

Microb Biotechnol. 2013 Mar;6(2):202-6. doi: 10.1111/1751-7915.12005. Epub 2012 Nov 20.

13.

An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform.

Rohe P, Venkanna D, Kleine B, Freudl R, Oldiges M.

Microb Cell Fact. 2012 Oct 31;11:144. doi: 10.1186/1475-2859-11-144.

14.

Genetic evidence for a tight cooperation of TatB and TatC during productive recognition of twin-arginine (Tat) signal peptides in Escherichia coli.

Lausberg F, Fleckenstein S, Kreutzenbeck P, Fröbel J, Rose P, Müller M, Freudl R.

PLoS One. 2012;7(6):e39867. doi: 10.1371/journal.pone.0039867. Epub 2012 Jun 26.

15.

A tetracycline inducible expression vector for Corynebacterium glutamicum allowing tightly regulable gene expression.

Lausberg F, Chattopadhyay AR, Heyer A, Eggeling L, Freudl R.

Plasmid. 2012 Sep;68(2):142-7. doi: 10.1016/j.plasmid.2012.05.001. Epub 2012 May 12.

PMID:
22587824
16.

Functional implementation of the posttranslational SecB-SecA protein-targeting pathway in Bacillus subtilis.

Diao L, Dong Q, Xu Z, Yang S, Zhou J, Freudl R.

Appl Environ Microbiol. 2012 Feb;78(3):651-9. doi: 10.1128/AEM.07209-11. Epub 2011 Nov 23.

17.

Twin-arginine translocation of methyl parathion hydrolase in Bacillus subtilis.

Yang C, Song C, Freudl R, Mulchandani A, Qiao C.

Environ Sci Technol. 2010 Oct 1;44(19):7607-12. doi: 10.1021/es100860k.

PMID:
20812717
18.

Improvement of Sec-dependent secretion of a heterologous model protein in Bacillus subtilis by saturation mutagenesis of the N-domain of the AmyE signal peptide.

Caspers M, Brockmeier U, Degering C, Eggert T, Freudl R.

Appl Microbiol Biotechnol. 2010 May;86(6):1877-85. doi: 10.1007/s00253-009-2405-x. Epub 2010 Jan 14.

PMID:
20077115
19.

Contributions of the pre- and pro-regions of a Staphylococcus hyicus lipase to secretion of a heterologous protein by Bacillus subtilis.

Kouwen TR, Nielsen AK, Denham EL, Dubois JY, Dorenbos R, Rasmussen MD, Quax WJ, Freudl R, van Dijl JM.

Appl Environ Microbiol. 2010 Feb;76(3):659-69. doi: 10.1128/AEM.01671-09. Epub 2009 Nov 30.

20.
21.

Export of methyl parathion hydrolase to the periplasm by the twin-arginine translocation pathway in Escherichia coli.

Yang C, Freudl R, Qiao C.

J Agric Food Chem. 2009 Oct 14;57(19):8901-5. doi: 10.1021/jf901739g.

PMID:
19754117
22.

A periplasmic, pyridoxal-5'-phosphate-dependent amino acid racemase in Pseudomonas taetrolens.

Matsui D, Oikawa T, Arakawa N, Osumi S, Lausberg F, Stäbler N, Freudl R, Eggeling L.

Appl Microbiol Biotechnol. 2009 Jul;83(6):1045-54. doi: 10.1007/s00253-009-1942-7. Epub 2009 Mar 20.

PMID:
19300994
23.

Modulation of thiol-disulfide oxidoreductases for increased production of disulfide-bond-containing proteins in Bacillus subtilis.

Kouwen TR, Dubois JY, Freudl R, Quax WJ, van Dijl JM.

Appl Environ Microbiol. 2008 Dec;74(24):7536-45. doi: 10.1128/AEM.00894-08. Epub 2008 Oct 24.

24.

Corynebacterium glutamicum possesses two secA homologous genes that are essential for viability.

Caspers M, Freudl R.

Arch Microbiol. 2008 Jun;189(6):605-10. doi: 10.1007/s00203-008-0351-0. Epub 2008 Feb 2.

PMID:
18246326
26.

Comparative analysis of twin-arginine (Tat)-dependent protein secretion of a heterologous model protein (GFP) in three different Gram-positive bacteria.

Meissner D, Vollstedt A, van Dijl JM, Freudl R.

Appl Microbiol Biotechnol. 2007 Sep;76(3):633-42. Epub 2007 Apr 24.

PMID:
17453196
27.

Escherichia coli twin arginine (Tat) mutant translocases possessing relaxed signal peptide recognition specificities.

Kreutzenbeck P, Kröger C, Lausberg F, Blaudeck N, Sprenger GA, Freudl R.

J Biol Chem. 2007 Mar 16;282(11):7903-11. Epub 2007 Jan 16.

28.

A disulfide bond-containing alkaline phosphatase triggers a BdbC-dependent secretion stress response in Bacillus subtilis.

Darmon E, Dorenbos R, Meens J, Freudl R, Antelmann H, Hecker M, Kuipers OP, Bron S, Quax WJ, Dubois JY, van Dijl JM.

Appl Environ Microbiol. 2006 Nov;72(11):6876-85.

29.

Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria.

Brockmeier U, Caspers M, Freudl R, Jockwer A, Noll T, Eggert T.

J Mol Biol. 2006 Sep 22;362(3):393-402. Epub 2006 Jul 26.

PMID:
16930615
30.

Heterologous expression and characterization of a novel branching enzyme from the thermoalkaliphilic anaerobic bacterium Anaerobranca gottschalkii.

Thiemann V, Saake B, Vollstedt A, Schäfer T, Puls J, Bertoldo C, Freudl R, Antranikian G.

Appl Microbiol Biotechnol. 2006 Aug;72(1):60-71. doi: 10.1007/s00253-005-0248-7. Epub 2006 Jan 12.

PMID:
16408175
31.

Isolation and characterization of bifunctional Escherichia coli TatA mutant proteins that allow efficient tat-dependent protein translocation in the absence of TatB.

Blaudeck N, Kreutzenbeck P, Müller M, Sprenger GA, Freudl R.

J Biol Chem. 2005 Feb 4;280(5):3426-32. Epub 2004 Nov 22.

32.
33.

Translocation of proteins across the cell envelope of Gram-positive bacteria.

van Wely KH, Swaving J, Freudl R, Driessen AJ.

FEMS Microbiol Rev. 2001 Aug;25(4):437-54. Review.

34.

Evaluation of parallel operated small-scale bubble columns for microbial process development using Staphylococcus carnosus.

Dilsen S, Paul W, Herforth D, Sandgathe A, Altenbach-Rehm J, Freudl R, Wandrey C, Weuster-Botz D.

J Biotechnol. 2001 Jun 1;88(1):77-84.

PMID:
11377767
35.

Specificity of signal peptide recognition in tat-dependent bacterial protein translocation.

Blaudeck N, Sprenger GA, Freudl R, Wiegert T.

J Bacteriol. 2001 Jan;183(2):604-10.

36.

Fed-batch production of recombinant human calcitonin precursor fusion protein using Staphylococcus carnosus as an expression-secretion system.

Dilsen S, Paul W, Sandgathe A, Tippe D, Freudl R, Thömmes J, Kula MR, Takors R, Wandrey C, Weuster-Botz D.

Appl Microbiol Biotechnol. 2000 Sep;54(3):361-9.

PMID:
11030573
37.

The carboxyl terminus of the Bacillus subtilis SecA is dispensable for protein secretion and viability.

van Wely KH, Swaving J, Klein M, Freudl R, Driessen AJ.

Microbiology. 2000 Oct;146 ( Pt 10):2573-81.

PMID:
11021932
38.

The LysE superfamily: topology of the lysine exporter LysE of Corynebacterium glutamicum, a paradyme for a novel superfamily of transmembrane solute translocators.

Vrljic M, Garg J, Bellmann A, Wachi S, Freudl R, Malecki MJ, Sahm H, Kozina VJ, Eggeling L, Saier MH Jr, Eggeling L, Saier MH Jr.

J Mol Microbiol Biotechnol. 1999 Nov;1(2):327-36.

PMID:
10943564
39.

Signal peptide peptidase- and ClpP-like proteins of Bacillus subtilis required for efficient translocation and processing of secretory proteins.

Bolhuis A, Matzen A, Hyyryläinen HL, Kontinen VP, Meima R, Chapuis J, Venema G, Bron S, Freudl R, van Dijl JM.

J Biol Chem. 1999 Aug 27;274(35):24585-92.

41.
42.
43.

Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase.

Herbort M, Klein M, Manting EH, Driessen AJ, Freudl R.

J Bacteriol. 1999 Jan;181(2):493-500.

46.
47.

Identification of the magnesium-binding domain of the high-affinity ATP-binding site of the Bacillus subtilis and Escherichia coli SecA protein.

van der Wolk JP, Klose M, de Wit JG, den Blaauwen T, Freudl R, Driessen AJ.

J Biol Chem. 1995 Aug 11;270(32):18975-82.

48.

Isolation and characterization of a Bacillus subtilis secA mutant allele conferring resistance to sodium azide.

Klein M, Hofmann B, Klose M, Freudl R.

FEMS Microbiol Lett. 1994 Dec 15;124(3):393-7.

PMID:
7851746
49.
50.

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