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Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids.

de Martín Garrido N, Crone MA, Ramlaul K, Simpson PA, Freemont PS, Aylett CHS.

Mol Microbiol. 2019 Oct 16. doi: 10.1111/mmi.14406. [Epub ahead of print]


The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology.

Wood TE, Howard SA, Förster A, Nolan LM, Manoli E, Bullen NP, Yau HCL, Hachani A, Hayward RD, Whitney JC, Vollmer W, Freemont PS, Filloux A.

Cell Rep. 2019 Oct 1;29(1):187-201.e7. doi: 10.1016/j.celrep.2019.08.094.


Author Correction: Building a global alliance of biofoundries.

Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS.

Nat Commun. 2019 Jul 11;10(1):3132. doi: 10.1038/s41467-019-10862-1.


Synthetic Biology and the United Nations.

Lai HE, Canavan C, Cameron L, Moore S, Danchenko M, Kuiken T, Sekeyová Z, Freemont PS.

Trends Biotechnol. 2019 Jun 27. pii: S0167-7799(19)30133-7. doi: 10.1016/j.tibtech.2019.05.011. [Epub ahead of print]


Building a global alliance of biofoundries.

Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS.

Nat Commun. 2019 May 9;10(1):2040. doi: 10.1038/s41467-019-10079-2. No abstract available. Erratum in: Nat Commun. 2019 Jul 11;10(1):3132.


Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation.

Suckling L, McFarlane C, Sawyer C, Chambers SP, Kitney RI, McClymont DW, Freemont PS.

Synth Syst Biotechnol. 2019 Jan 22;4(1):57-66. doi: 10.1016/j.synbio.2019.01.002. eCollection 2019 Mar.


Utilising datasheets for the informed automated design and build of a synthetic metabolic pathway.

Exley K, Reynolds CR, Suckling L, Chee SM, Tsipa A, Freemont PS, McClymont D, Kitney RI.

J Biol Eng. 2019 Jan 18;13:8. doi: 10.1186/s13036-019-0141-z. eCollection 2019.


Whole-Cell Biosensor with Tunable Limit of Detection Enables Low-Cost Agglutination Assays for Medical Diagnostic Applications.

Kylilis N, Riangrungroj P, Lai HE, Salema V, Fernández LÁ, Stan GV, Freemont PS, Polizzi KM.

ACS Sens. 2019 Feb 22;4(2):370-378. doi: 10.1021/acssensors.8b01163. Epub 2019 Jan 25.


Structural basis for recognition and repair of the 3'-phosphate by NExo, a base excision DNA repair nuclease from Neisseria meningitidis.

Silhan J, Zhao Q, Boura E, Thomson H, Förster A, Tang CM, Freemont PS, Baldwin GS.

Nucleic Acids Res. 2018 Dec 14;46(22):11980-11989. doi: 10.1093/nar/gky934.


Rapid Prototyping Platform for Saccharomyces cerevisiae Using Computer-Aided Genetic Design Enabled by Parallel Software and Workcell Platform Development.

Rajakumar PD, Gowers GF, Suckling L, Foster A, Ellis T, Kitney RI, McClymont DW, Freemont PS.

SLAS Technol. 2019 Jun;24(3):291-297. doi: 10.1177/2472630318798304. Epub 2018 Aug 30.


Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria.

Moore SJ, MacDonald JT, Wienecke S, Ishwarbhai A, Tsipa A, Aw R, Kylilis N, Bell DJ, McClymont DW, Jensen K, Polizzi KM, Biedendieck R, Freemont PS.

Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4340-E4349. doi: 10.1073/pnas.1715806115. Epub 2018 Apr 17.


Corrigendum: Inhibitor Selectivity for Cyclin-Dependent Kinase 7: A Structural, Thermodynamic, and Modelling Study.

Hazel P, Kroll SHB, Bondke A, Barbazanges M, Patel H, Fuchter MJ, Coombes RC, Ali S, Barrett AGM, Freemont PS.

ChemMedChem. 2018 Jan 22;13(2):207. doi: 10.1002/cmdc.201700826. Epub 2018 Jan 16. No abstract available.


Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa.

Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PS.

Structure. 2018 Feb 6;26(2):329-336.e3. doi: 10.1016/j.str.2017.12.005. Epub 2018 Jan 4.


A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.

Wen KY, Cameron L, Chappell J, Jensen K, Bell DJ, Kelwick R, Kopniczky M, Davies JC, Filloux A, Freemont PS.

ACS Synth Biol. 2017 Dec 15;6(12):2293-2301. doi: 10.1021/acssynbio.7b00219. Epub 2017 Oct 5.


Current and future therapies for Pseudomonas aeruginosa infection in patients with cystic fibrosis.

Smith WD, Bardin E, Cameron L, Edmondson CL, Farrant KV, Martin I, Murphy RA, Soren O, Turnbull AR, Wierre-Gore N, Alton EW, Bundy JG, Bush A, Connett GJ, Faust SN, Filloux A, Freemont PS, Jones AL, Takats Z, Webb JS, Williams HD, Davies JC.

FEMS Microbiol Lett. 2017 Aug 1;364(14). doi: 10.1093/femsle/fnx121. Review.


The AAA+ ATPase p97, a cellular multitool.

Stach L, Freemont PS.

Biochem J. 2017 Aug 17;474(17):2953-2976. doi: 10.1042/BCJ20160783. Review.


Cell-free synthetic biology for in vitro prototype engineering.

Moore SJ, MacDonald JT, Freemont PS.

Biochem Soc Trans. 2017 Jun 15;45(3):785-791. doi: 10.1042/BST20170011. Review.


With all due respect to Maholo, lab automation isn't anthropomorphic.

McClymont DW, Freemont PS.

Nat Biotechnol. 2017 Apr 11;35(4):312-314. doi: 10.1038/nbt.3795. No abstract available.


Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool.

Moore SJ, Lai HE, Needham H, Polizzi KM, Freemont PS.

Biotechnol J. 2017 Apr;12(4). doi: 10.1002/biot.201600678. Epub 2017 Feb 24.


Inhibitor Selectivity for Cyclin-Dependent Kinase 7: A Structural, Thermodynamic, and Modelling Study.

Hazel P, Kroll SH, Bondke A, Barbazanges M, Patel H, Fuchter MJ, Coombes RC, Ali S, Barrett AG, Freemont PS.

ChemMedChem. 2017 Mar 7;12(5):372-380. doi: 10.1002/cmdc.201600535. Epub 2017 Feb 6. Erratum in: ChemMedChem. 2018 Jan 22;13(2):207.


Opportunities for applying whole-cell bioreporters towards parasite detection.

Webb AJ, Kelwick R, Freemont PS.

Microb Biotechnol. 2017 Mar;10(2):244-249. doi: 10.1111/1751-7915.12604. Epub 2017 Jan 26. No abstract available.


Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.

Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM.

Biotechnol Bioeng. 2017 Jun;114(6):1290-1300. doi: 10.1002/bit.26254. Epub 2017 Feb 23.


Computational protein design with backbone plasticity.

MacDonald JT, Freemont PS.

Biochem Soc Trans. 2016 Oct 15;44(5):1523-1529. Epub 2016 Oct 19. Review.


EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.

Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS.

ACS Synth Biol. 2016 Oct 21;5(10):1059-1069. Epub 2016 May 2.


Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements.

Kelwick R, Webb AJ, MacDonald JT, Freemont PS.

Metab Eng. 2016 Nov;38:370-381. doi: 10.1016/j.ymben.2016.09.008. Epub 2016 Sep 30.


Synthetic beta-solenoid proteins with the fragment-free computational design of a beta-hairpin extension.

MacDonald JT, Kabasakal BV, Godding D, Kraatz S, Henderson L, Barber J, Freemont PS, Murray JW.

Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10346-51. doi: 10.1073/pnas.1525308113. Epub 2016 Aug 29.


TssA forms a gp6-like ring attached to the type VI secretion sheath.

Planamente S, Salih O, Manoli E, Albesa-Jové D, Freemont PS, Filloux A.

EMBO J. 2016 Aug 1;35(15):1613-27. doi: 10.15252/embj.201694024. Epub 2016 Jun 10.


Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain.

Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T.

Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 10.1073/pnas.1522985113. Epub 2016 May 31.


A protease-based biosensor for the detection of schistosome cercariae.

Webb AJ, Kelwick R, Doenhoff MJ, Kylilis N, MacDonald JT, Wen KY, McKeown C, Baldwin G, Ellis T, Jensen K, Freemont PS.

Sci Rep. 2016 Apr 19;6:24725. doi: 10.1038/srep24725.


Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582.

Florea M, Reeve B, Abbott J, Freemont PS, Ellis T.

Sci Rep. 2016 Mar 24;6:23635. doi: 10.1038/srep23635.


Delineation of metabolic gene clusters in plant genomes by chromatin signatures.

Yu N, Nützmann HW, MacDonald JT, Moore B, Field B, Berriri S, Trick M, Rosser SJ, Kumar SV, Freemont PS, Osbourn A.

Nucleic Acids Res. 2016 Mar 18;44(5):2255-65. doi: 10.1093/nar/gkw100. Epub 2016 Feb 18.


The N-terminal Region of the Ubiquitin Regulatory X (UBX) Domain-containing Protein 1 (UBXD1) Modulates Interdomain Communication within the Valosin-containing Protein p97.

Trusch F, Matena A, Vuk M, Koerver L, Knævelsrud H, Freemont PS, Meyer H, Bayer P.

J Biol Chem. 2015 Dec 4;290(49):29414-27. doi: 10.1074/jbc.M115.680686. Epub 2015 Oct 16.


Multilevel Regulation and Translational Switches in Synthetic Biology.

Kopniczky MB, Moore SJ, Freemont PS.

IEEE Trans Biomed Circuits Syst. 2015 Aug;9(4):485-96. Epub 2015 Aug 27. Review.


New quantitative approaches reveal the spatial preference of nuclear compartments in mammalian fibroblasts.

Weston DJ, Russell RA, Batty E, Jensen K, Stephens DA, Adams NM, Freemont PS.

J R Soc Interface. 2015 Mar 6;12(104):20140894. doi: 10.1098/rsif.2014.0894.


Complex structure and biochemical characterization of the Staphylococcus aureus cyclic diadenylate monophosphate (c-di-AMP)-binding protein PstA, the founding member of a new signal transduction protein family.

Campeotto I, Zhang Y, Mladenov MG, Freemont PS, Gründling A.

J Biol Chem. 2015 Jan 30;290(5):2888-901. doi: 10.1074/jbc.M114.621789. Epub 2014 Dec 11.


Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.

Förster A, Planamente S, Manoli E, Lossi NS, Freemont PS, Filloux A.

J Biol Chem. 2014 Nov 21;289(47):33032-43. doi: 10.1074/jbc.M114.600510. Epub 2014 Oct 10.


Structural and mechanistic insight into the Listeria monocytogenes two-enzyme lipoteichoic acid synthesis system.

Campeotto I, Percy MG, MacDonald JT, Förster A, Freemont PS, Gründling A.

J Biol Chem. 2014 Oct 10;289(41):28054-69. doi: 10.1074/jbc.M114.590570. Epub 2014 Aug 15.


R2oDNA designer: computational design of biologically neutral synthetic DNA sequences.

Casini A, Christodoulou G, Freemont PS, Baldwin GS, Ellis T, MacDonald JT.

ACS Synth Biol. 2014 Aug 15;3(8):525-8. doi: 10.1021/sb4001323. Epub 2014 Feb 11.


The p97-FAF1 protein complex reveals a common mode of p97 adaptor binding.

Ewens CA, Panico S, Kloppsteck P, McKeown C, Ebong IO, Robinson C, Zhang X, Freemont PS.

J Biol Chem. 2014 Apr 25;289(17):12077-84. doi: 10.1074/jbc.M114.559591. Epub 2014 Mar 11.


Inter-ring rotations of AAA ATPase p97 revealed by electron cryomicroscopy.

Yeung HO, Förster A, Bebeacua C, Niwa H, Ewens C, McKeown C, Zhang X, Freemont PS.

Open Biol. 2014 Mar 5;4:130142. doi: 10.1098/rsob.130142.


One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy.

Casini A, MacDonald JT, De Jonghe J, Christodoulou G, Freemont PS, Baldwin GS, Ellis T.

Nucleic Acids Res. 2014 Jan;42(1):e7. doi: 10.1093/nar/gkt915. Epub 2013 Oct 22.


Design of a prototype flow microreactor for synthetic biology in vitro.

Boehm CR, Freemont PS, Ces O.

Lab Chip. 2013 Sep 7;13(17):3426-32. doi: 10.1039/c3lc50231g. Epub 2013 Jul 10.


Validating a Coarse-Grained Potential Energy Function through Protein Loop Modelling.

Macdonald JT, Kelley LA, Freemont PS.

PLoS One. 2013 Jun 18;8(6):e65770. doi: 10.1371/journal.pone.0065770. Print 2013.


Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.

Chappell J, Jensen K, Freemont PS.

Nucleic Acids Res. 2013 Mar 1;41(5):3471-81. doi: 10.1093/nar/gkt052. Epub 2013 Jan 31.


The Salmonella kinase SteC targets the MAP kinase MEK to regulate the host actin cytoskeleton.

Odendall C, Rolhion N, Förster A, Poh J, Lamont DJ, Liu M, Freemont PS, Catling AD, Holden DW.

Cell Host Microbe. 2012 Nov 15;12(5):657-68. doi: 10.1016/j.chom.2012.09.011.


Structural basis for the recognition and cleavage of abasic DNA in Neisseria meningitidis.

Lu D, Silhan J, MacDonald JT, Carpenter EP, Jensen K, Tang CM, Baldwin GS, Freemont PS.

Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16852-7. doi: 10.1073/pnas.1206563109. Epub 2012 Oct 3.


Analysis of spatial point patterns in nuclear biology.

Weston DJ, Adams NM, Russell RA, Stephens DA, Freemont PS.

PLoS One. 2012;7(5):e36841. doi: 10.1371/journal.pone.0036841. Epub 2012 May 16.


A network of enzymes involved in repair of oxidative DNA damage in Neisseria meningitidis.

Nagorska K, Silhan J, Li Y, Pelicic V, Freemont PS, Baldwin GS, Tang CM.

Mol Microbiol. 2012 Mar;83(5):1064-1079. doi: 10.1111/j.1365-2958.2012.07989.x. Epub 2012 Feb 15.


The role of the N-domain in the ATPase activity of the mammalian AAA ATPase p97/VCP.

Niwa H, Ewens CA, Tsang C, Yeung HO, Zhang X, Freemont PS.

J Biol Chem. 2012 Mar 9;287(11):8561-70. doi: 10.1074/jbc.M111.302778. Epub 2012 Jan 23.


Distinct conformations of the protein complex p97-Ufd1-Npl4 revealed by electron cryomicroscopy.

Bebeacua C, Förster A, McKeown C, Meyer HH, Zhang X, Freemont PS.

Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1098-103. doi: 10.1073/pnas.1114341109. Epub 2012 Jan 9.

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