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Structural basis for recognition and repair of the 3'-phosphate by NExo, a base excision DNA repair nuclease from Neisseria meningitidis.

Silhan J, Zhao Q, Boura E, Thomson H, Förster A, Tang CM, Freemont PS, Baldwin GS.

Nucleic Acids Res. 2018 Oct 17. doi: 10.1093/nar/gky934. [Epub ahead of print]


Rapid Prototyping Platform for Saccharomyces cerevisiae Using Computer-Aided Genetic Design Enabled by Parallel Software and Workcell Platform Development.

Rajakumar PD, Gowers GF, Suckling L, Foster A, Ellis T, Kitney RI, McClymont DW, Freemont PS.

SLAS Technol. 2018 Aug 30:2472630318798304. doi: 10.1177/2472630318798304. [Epub ahead of print]


EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.

Lai HE, Moore S, Polizzi K, Freemont P.

Methods Mol Biol. 2018;1772:429-444. doi: 10.1007/978-1-4939-7795-6_25.


Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria.

Moore SJ, MacDonald JT, Wienecke S, Ishwarbhai A, Tsipa A, Aw R, Kylilis N, Bell DJ, McClymont DW, Jensen K, Polizzi KM, Biedendieck R, Freemont PS.

Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4340-E4349. doi: 10.1073/pnas.1715806115. Epub 2018 Apr 17.


Corrigendum: Inhibitor Selectivity for Cyclin-Dependent Kinase 7: A Structural, Thermodynamic, and Modelling Study.

Hazel P, Kroll SHB, Bondke A, Barbazanges M, Patel H, Fuchter MJ, Coombes RC, Ali S, Barrett AGM, Freemont PS.

ChemMedChem. 2018 Jan 22;13(2):207. doi: 10.1002/cmdc.201700826. Epub 2018 Jan 16. No abstract available.


Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa.

Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PS.

Structure. 2018 Feb 6;26(2):329-336.e3. doi: 10.1016/j.str.2017.12.005. Epub 2018 Jan 4.


A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.

Wen KY, Cameron L, Chappell J, Jensen K, Bell DJ, Kelwick R, Kopniczky M, Davies JC, Filloux A, Freemont PS.

ACS Synth Biol. 2017 Dec 15;6(12):2293-2301. doi: 10.1021/acssynbio.7b00219. Epub 2017 Oct 5.


Current and future therapies for Pseudomonas aeruginosa infection in patients with cystic fibrosis.

Smith WD, Bardin E, Cameron L, Edmondson CL, Farrant KV, Martin I, Murphy RA, Soren O, Turnbull AR, Wierre-Gore N, Alton EW, Bundy JG, Bush A, Connett GJ, Faust SN, Filloux A, Freemont PS, Jones AL, Takats Z, Webb JS, Williams HD, Davies JC.

FEMS Microbiol Lett. 2017 Aug 1;364(14). doi: 10.1093/femsle/fnx121. Review.


The AAA+ ATPase p97, a cellular multitool.

Stach L, Freemont PS.

Biochem J. 2017 Aug 17;474(17):2953-2976. doi: 10.1042/BCJ20160783. Review.


Cell-free synthetic biology for in vitro prototype engineering.

Moore SJ, MacDonald JT, Freemont PS.

Biochem Soc Trans. 2017 Jun 15;45(3):785-791. doi: 10.1042/BST20170011. Review.


With all due respect to Maholo, lab automation isn't anthropomorphic.

McClymont DW, Freemont PS.

Nat Biotechnol. 2017 Apr 11;35(4):312-314. doi: 10.1038/nbt.3795. No abstract available.


Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool.

Moore SJ, Lai HE, Needham H, Polizzi KM, Freemont PS.

Biotechnol J. 2017 Apr;12(4). doi: 10.1002/biot.201600678. Epub 2017 Feb 24.


Inhibitor Selectivity for Cyclin-Dependent Kinase 7: A Structural, Thermodynamic, and Modelling Study.

Hazel P, Kroll SH, Bondke A, Barbazanges M, Patel H, Fuchter MJ, Coombes RC, Ali S, Barrett AG, Freemont PS.

ChemMedChem. 2017 Mar 7;12(5):372-380. doi: 10.1002/cmdc.201600535. Epub 2017 Feb 6. Erratum in: ChemMedChem. 2018 Jan 22;13(2):207.


Opportunities for applying whole-cell bioreporters towards parasite detection.

Webb AJ, Kelwick R, Freemont PS.

Microb Biotechnol. 2017 Mar;10(2):244-249. doi: 10.1111/1751-7915.12604. Epub 2017 Jan 26. No abstract available.


Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.

Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM.

Biotechnol Bioeng. 2017 Jun;114(6):1290-1300. doi: 10.1002/bit.26254. Epub 2017 Feb 23.


Computational protein design with backbone plasticity.

MacDonald JT, Freemont PS.

Biochem Soc Trans. 2016 Oct 15;44(5):1523-1529. Epub 2016 Oct 19. Review.


EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.

Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS.

ACS Synth Biol. 2016 Oct 21;5(10):1059-1069. Epub 2016 May 2.


Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements.

Kelwick R, Webb AJ, MacDonald JT, Freemont PS.

Metab Eng. 2016 Nov;38:370-381. doi: 10.1016/j.ymben.2016.09.008. Epub 2016 Sep 30.


Synthetic beta-solenoid proteins with the fragment-free computational design of a beta-hairpin extension.

MacDonald JT, Kabasakal BV, Godding D, Kraatz S, Henderson L, Barber J, Freemont PS, Murray JW.

Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10346-51. doi: 10.1073/pnas.1525308113. Epub 2016 Aug 29.


Structural biology: Baseplates in contractile machines.

Filloux A, Freemont P.

Nat Microbiol. 2016 Jun 24;1(7):16104. doi: 10.1038/nmicrobiol.2016.104. No abstract available.


The second messenger c-di-AMP inhibits the osmolyte uptake system OpuC in Staphylococcus aureus.

Schuster CF, Bellows LE, Tosi T, Campeotto I, Corrigan RM, Freemont P, Gründling A.

Sci Signal. 2016 Aug 16;9(441):ra81. doi: 10.1126/scisignal.aaf7279.


TssA forms a gp6-like ring attached to the type VI secretion sheath.

Planamente S, Salih O, Manoli E, Albesa-Jové D, Freemont PS, Filloux A.

EMBO J. 2016 Aug 1;35(15):1613-27. doi: 10.15252/embj.201694024. Epub 2016 Jun 10.


The Foundry: the DNA synthesis and construction Foundry at Imperial College.

Chambers S, Kitney R, Freemont P.

Biochem Soc Trans. 2016 Jun 15;44(3):687-8. doi: 10.1042/BST20160007.


Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain.

Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T.

Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 10.1073/pnas.1522985113. Epub 2016 May 31.


Pseudomonas aeruginosa infection in cystic fibrosis: pathophysiological mechanisms and therapeutic approaches.

Lund-Palau H, Turnbull AR, Bush A, Bardin E, Cameron L, Soren O, Wierre-Gore N, Alton EW, Bundy JG, Connett G, Faust SN, Filloux A, Freemont P, Jones A, Khoo V, Morales S, Murphy R, Pabary R, Simbo A, Schelenz S, Takats Z, Webb J, Williams HD, Davies JC.

Expert Rev Respir Med. 2016 Jun;10(6):685-97. doi: 10.1080/17476348.2016.1177460. Epub 2016 May 13. Review.


A protease-based biosensor for the detection of schistosome cercariae.

Webb AJ, Kelwick R, Doenhoff MJ, Kylilis N, MacDonald JT, Wen KY, McKeown C, Baldwin G, Ellis T, Jensen K, Freemont PS.

Sci Rep. 2016 Apr 19;6:24725. doi: 10.1038/srep24725.


Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582.

Florea M, Reeve B, Abbott J, Freemont PS, Ellis T.

Sci Rep. 2016 Mar 24;6:23635. doi: 10.1038/srep23635.


Delineation of metabolic gene clusters in plant genomes by chromatin signatures.

Yu N, Nützmann HW, MacDonald JT, Moore B, Field B, Berriri S, Trick M, Rosser SJ, Kumar SV, Freemont PS, Osbourn A.

Nucleic Acids Res. 2016 Mar 18;44(5):2255-65. doi: 10.1093/nar/gkw100. Epub 2016 Feb 18.


The N-terminal Region of the Ubiquitin Regulatory X (UBX) Domain-containing Protein 1 (UBXD1) Modulates Interdomain Communication within the Valosin-containing Protein p97.

Trusch F, Matena A, Vuk M, Koerver L, Knævelsrud H, Freemont PS, Meyer H, Bayer P.

J Biol Chem. 2015 Dec 4;290(49):29414-27. doi: 10.1074/jbc.M115.680686. Epub 2015 Oct 16.


Multilevel Regulation and Translational Switches in Synthetic Biology.

Kopniczky MB, Moore SJ, Freemont PS.

IEEE Trans Biomed Circuits Syst. 2015 Aug;9(4):485-96. Epub 2015 Aug 27. Review.


A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli.

Kelwick R, Kopniczky M, Bower I, Chi W, Chin MH, Fan S, Pilcher J, Strutt J, Webb AJ, Jensen K, Stan GB, Kitney R, Freemont P.

PLoS One. 2015 Feb 20;10(2):e0117202. doi: 10.1371/journal.pone.0117202. eCollection 2015.


New quantitative approaches reveal the spatial preference of nuclear compartments in mammalian fibroblasts.

Weston DJ, Russell RA, Batty E, Jensen K, Stephens DA, Adams NM, Freemont PS.

J R Soc Interface. 2015 Mar 6;12(104):20140894. doi: 10.1098/rsif.2014.0894.


Developments in the tools and methodologies of synthetic biology.

Kelwick R, MacDonald JT, Webb AJ, Freemont P.

Front Bioeng Biotechnol. 2014 Nov 26;2:60. doi: 10.3389/fbioe.2014.00060. eCollection 2014. Review.


Complex structure and biochemical characterization of the Staphylococcus aureus cyclic diadenylate monophosphate (c-di-AMP)-binding protein PstA, the founding member of a new signal transduction protein family.

Campeotto I, Zhang Y, Mladenov MG, Freemont PS, Gründling A.

J Biol Chem. 2015 Jan 30;290(5):2888-901. doi: 10.1074/jbc.M114.621789. Epub 2014 Dec 11.


Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.

Förster A, Planamente S, Manoli E, Lossi NS, Freemont PS, Filloux A.

J Biol Chem. 2014 Nov 21;289(47):33032-43. doi: 10.1074/jbc.M114.600510. Epub 2014 Oct 10.


How synthetic biology will reconsider natural bioluminescence and its applications.

Reeve B, Sanderson T, Ellis T, Freemont P.

Adv Biochem Eng Biotechnol. 2014;145:3-30. doi: 10.1007/978-3-662-43619-6_1. Review.


Structural and mechanistic insight into the Listeria monocytogenes two-enzyme lipoteichoic acid synthesis system.

Campeotto I, Percy MG, MacDonald JT, Förster A, Freemont PS, Gründling A.

J Biol Chem. 2014 Oct 10;289(41):28054-69. doi: 10.1074/jbc.M114.590570. Epub 2014 Aug 15.


R2oDNA designer: computational design of biologically neutral synthetic DNA sequences.

Casini A, Christodoulou G, Freemont PS, Baldwin GS, Ellis T, MacDonald JT.

ACS Synth Biol. 2014 Aug 15;3(8):525-8. doi: 10.1021/sb4001323. Epub 2014 Feb 11.


Co-culture systems and technologies: taking synthetic biology to the next level.

Goers L, Freemont P, Polizzi KM.

J R Soc Interface. 2014 Jul 6;11(96). pii: 20140065. doi: 10.1098/rsif.2014.0065. Review.


The p97-FAF1 protein complex reveals a common mode of p97 adaptor binding.

Ewens CA, Panico S, Kloppsteck P, McKeown C, Ebong IO, Robinson C, Zhang X, Freemont PS.

J Biol Chem. 2014 Apr 25;289(17):12077-84. doi: 10.1074/jbc.M114.559591. Epub 2014 Mar 11.


Inter-ring rotations of AAA ATPase p97 revealed by electron cryomicroscopy.

Yeung HO, Förster A, Bebeacua C, Niwa H, Ewens C, McKeown C, Zhang X, Freemont PS.

Open Biol. 2014 Mar 5;4:130142. doi: 10.1098/rsob.130142.


One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy.

Casini A, MacDonald JT, De Jonghe J, Christodoulou G, Freemont PS, Baldwin GS, Ellis T.

Nucleic Acids Res. 2014 Jan;42(1):e7. doi: 10.1093/nar/gkt915. Epub 2013 Oct 22.


In vivo and in vitro characterization of σ70 constitutive promoters by real-time PCR and fluorescent measurements.

Chappell J, Freemont P.

Methods Mol Biol. 2013;1073:61-74. doi: 10.1007/978-1-62703-625-2_7.


Design of a prototype flow microreactor for synthetic biology in vitro.

Boehm CR, Freemont PS, Ces O.

Lab Chip. 2013 Sep 7;13(17):3426-32. doi: 10.1039/c3lc50231g. Epub 2013 Jul 10.


Validating a Coarse-Grained Potential Energy Function through Protein Loop Modelling.

Macdonald JT, Kelley LA, Freemont PS.

PLoS One. 2013 Jun 18;8(6):e65770. doi: 10.1371/journal.pone.0065770. Print 2013.


Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.

Chappell J, Jensen K, Freemont PS.

Nucleic Acids Res. 2013 Mar 1;41(5):3471-81. doi: 10.1093/nar/gkt052. Epub 2013 Jan 31.


The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI secretion system forms a bacteriophage tail sheathlike structure.

Lossi NS, Manoli E, Förster A, Dajani R, Pape T, Freemont P, Filloux A.

J Biol Chem. 2013 Mar 15;288(11):7536-48. doi: 10.1074/jbc.M112.439273. Epub 2013 Jan 22.


The Salmonella kinase SteC targets the MAP kinase MEK to regulate the host actin cytoskeleton.

Odendall C, Rolhion N, Förster A, Poh J, Lamont DJ, Liu M, Freemont PS, Catling AD, Holden DW.

Cell Host Microbe. 2012 Nov 15;12(5):657-68. doi: 10.1016/j.chom.2012.09.011.


Structural basis for the recognition and cleavage of abasic DNA in Neisseria meningitidis.

Lu D, Silhan J, MacDonald JT, Carpenter EP, Jensen K, Tang CM, Baldwin GS, Freemont PS.

Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16852-7. doi: 10.1073/pnas.1206563109. Epub 2012 Oct 3.


The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system.

Lossi NS, Manoli E, Simpson P, Jones C, Hui K, Dajani R, Coulthurst SJ, Freemont P, Filloux A.

Mol Microbiol. 2012 Oct;86(2):437-56. doi: 10.1111/j.1365-2958.2012.08204.x. Epub 2012 Aug 29.

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