Send to

Choose Destination
Appl Environ Microbiol. 1999 Feb;65(2):648-51.

Detection of methanotrophs in groundwater by PCR.

Author information

Department of Biological Sciences and Great Lakes Water Institute, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, USA.


Methanotrophic bacteria have significant potential for bioremediation, which would require methods for monitoring the presence and activity of these organisms in environmental samples. In this study, PCR was used to detect methanotrophic bacteria. Primers were designed on the basis of a partial sequence of pmoA, which encodes one of the proteins of the particulate methane monooxygenase. Specific amplification of a portion of pmoA was obtained with template DNA isolated from lab strains of methanotrophs. A pmoA product was also obtained by using DNA from groundwater. The identity of the PCR product was confirmed by sequencing or by amplification with a nested primer. Reverse transcriptase PCR detected pmoA mRNA.


Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center