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Artif Cells Nanomed Biotechnol. 2017 Nov;45(7):1343-1349. doi: 10.1080/21691401.2016.1236804. Epub 2016 Sep 30.

The effect of purmorphamine on differentiation of endometrial stem cells into osteoblast-like cells on collagen/hydroxyapatite scaffolds.

Author information

1
a Department of Oral and Maxillofacial Surgery , School of Dentistry, Tehran University of Medical Sciences , Tehran , Iran.
2
b Craniomaxillo Facial Research Center, Tehran University of Medical Sciences , Tehran , Iran.
3
c Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences , Tehran , Iran.
4
d Department of Periodontics , School of Dentistry, Qazvin University of Medical Sciences , Qazvin , Iran.
5
e Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
6
f Department of Genetics, Science and Research Branch , Islamic Azad University , Tehran , Iran.
7
g Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences , Tehran , Iran.
8
h Department of Biotechnology, School of Advanced Technologies in Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
9
i Department of Biomedical Engineering , Wake Forest university ? Virginia Tech, Winston Salem , NC , USA.

Abstract

We assessed the effect of purmorphamine along with collagen/hydroxyapatite scaffold in inducing osteogenesis of human endometrial stem cells (hEnSCs). The adhesion, viability, proliferation, and differentiation of cells on scaffold were assayed with SEM, MTT, real time-PCR, and ALP assay, respectively. The results were shown good integration of cells with scaffold. Also, qRT-PCR of differentiated cells shows that osteoblast cell markers are expressed after 21d in 2D and scaffold groups while in the scaffold group the expression of these markers were higher than the 2D group. Based on our findings, collagen/hydroxyapatite scaffold with PMA has the potential role in osteogenic differentiation of hEnSCs.

KEYWORDS:

bone tissue engineering; collagen/hydroxyapatite scaffold; hEnSCs; purmorphamin

PMID:
27686538
DOI:
10.1080/21691401.2016.1236804
[Indexed for MEDLINE]

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