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Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry.

Arttamangkul S, Plazek A, Platt EJ, Jin H, Murray TF, Birdsong WT, Rice KC, Farrens DL, Williams JT.

Elife. 2019 Oct 7;8. pii: e49319. doi: 10.7554/eLife.49319.


Structure of a Signaling Cannabinoid Receptor 1-G Protein Complex.

Krishna Kumar K, Shalev-Benami M, Robertson MJ, Hu H, Banister SD, Hollingsworth SA, Latorraca NR, Kato HE, Hilger D, Maeda S, Weis WI, Farrens DL, Dror RO, Malhotra SV, Kobilka BK, Skiniotis G.

Cell. 2019 Jan 24;176(3):448-458.e12. doi: 10.1016/j.cell.2018.11.040. Epub 2019 Jan 10.


Single Proteoliposome High-Content Analysis Reveals Differences in the Homo-Oligomerization of GPCRs.

Walsh SM, Mathiasen S, Christensen SM, Fay JF, King C, Provasi D, Borrero E, Rasmussen SGF, Fung JJ, Filizola M, Hristova K, Kobilka B, Farrens DL, Stamou D.

Biophys J. 2018 Jul 17;115(2):300-312. doi: 10.1016/j.bpj.2018.05.036.


Purification of Functional CB1 and Analysis by Site-Directed Fluorescence Labeling Methods.

Fay JF, Farrens DL.

Methods Enzymol. 2017;593:343-370. doi: 10.1016/bs.mie.2017.06.026. Epub 2017 Jul 17.


Decay of an active GPCR: Conformational dynamics govern agonist rebinding and persistence of an active, yet empty, receptor state.

Schafer CT, Fay JF, Janz JM, Farrens DL.

Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):11961-11966. Epub 2016 Oct 4.


Biosensor reveals multiple sources for mitochondrial NAD⁺.

Cambronne XA, Stewart ML, Kim D, Jones-Brunette AM, Morgan RK, Farrens DL, Cohen MS, Goodman RH.

Science. 2016 Jun 17;352(6292):1474-7. doi: 10.1126/science.aad5168.


Evidence that the Rhodopsin Kinase (GRK1) N-Terminus and the Transducin Gα C-Terminus Interact with the Same "Hydrophobic Patch" on Rhodopsin TM5.

Jones Brunette AM, Sinha A, David L, Farrens DL.

Biochemistry. 2016 Jun 7;55(22):3123-35. doi: 10.1021/acs.biochem.6b00328. Epub 2016 May 26.


Retinal Attachment Instability Is Diversified among Mammalian Melanopsins.

Tsukamoto H, Kubo Y, Farrens DL, Koyanagi M, Terakita A, Furutani Y.

J Biol Chem. 2015 Nov 6;290(45):27176-87. doi: 10.1074/jbc.M115.666305. Epub 2015 Sep 28.


Structural dynamics and energetics underlying allosteric inactivation of the cannabinoid receptor CB1.

Fay JF, Farrens DL.

Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8469-74. doi: 10.1073/pnas.1500895112. Epub 2015 Jun 22.


Conformational selection and equilibrium governs the ability of retinals to bind opsin.

Schafer CT, Farrens DL.

J Biol Chem. 2015 Feb 13;290(7):4304-18. doi: 10.1074/jbc.M114.603134. Epub 2014 Dec 1.


Distance mapping in proteins using fluorescence spectroscopy: tyrosine, like tryptophan, quenches bimane fluorescence in a distance-dependent manner.

Jones Brunette AM, Farrens DL.

Biochemistry. 2014 Oct 14;53(40):6290-301. doi: 10.1021/bi500493r. Epub 2014 Oct 1.


Nanoscale high-content analysis using compositional heterogeneities of single proteoliposomes.

Mathiasen S, Christensen SM, Fung JJ, Rasmussen SG, Fay JF, Jorgensen SK, Veshaguri S, Farrens DL, Kiskowski M, Kobilka B, Stamou D.

Nat Methods. 2014 Sep;11(9):931-4. doi: 10.1038/nmeth.3062. Epub 2014 Aug 3.


Rhodopsin TM6 can interact with two separate and distinct sites on arrestin: evidence for structural plasticity and multiple docking modes in arrestin-rhodopsin binding.

Sinha A, Jones Brunette AM, Fay JF, Schafer CT, Farrens DL.

Biochemistry. 2014 May 27;53(20):3294-307. doi: 10.1021/bi401534y. Epub 2014 May 13.


The membrane proximal region of the cannabinoid receptor CB1 N-terminus can allosterically modulate ligand affinity.

Fay JF, Farrens DL.

Biochemistry. 2013 Nov 19;52(46):8286-94. doi: 10.1021/bi400842k. Epub 2013 Nov 8.


Fluorescence spectroscopy of rhodopsins: insights and approaches.

Alexiev U, Farrens DL.

Biochim Biophys Acta. 2014 May;1837(5):694-709. doi: 10.1016/j.bbabio.2013.10.008. Epub 2013 Oct 29. Review.


A constitutively activating mutation alters the dynamics and energetics of a key conformational change in a ligand-free G protein-coupled receptor.

Tsukamoto H, Farrens DL.

J Biol Chem. 2013 Sep 27;288(39):28207-16. doi: 10.1074/jbc.M113.472464. Epub 2013 Aug 12.


Rhodopsin in nanodiscs has native membrane-like photointermediates.

Tsukamoto H, Szundi I, Lewis JW, Farrens DL, Kliger DS.

Biochemistry. 2011 Jun 7;50(22):5086-91. doi: 10.1021/bi200391a. Epub 2011 May 13.


What site-directed labeling studies tell us about the mechanism of rhodopsin activation and G-protein binding.

Farrens DL.

Photochem Photobiol Sci. 2010 Nov;9(11):1466-74. doi: 10.1039/c0pp00283f. Epub 2010 Oct 21. Review.


Distance mapping in proteins using fluorescence spectroscopy: the tryptophan-induced quenching (TrIQ) method.

Mansoor SE, Dewitt MA, Farrens DL.

Biochemistry. 2010 Nov 16;49(45):9722-31. doi: 10.1021/bi100907m. Epub 2010 Oct 26.


Monomeric rhodopsin is the minimal functional unit required for arrestin binding.

Tsukamoto H, Sinha A, DeWitt M, Farrens DL.

J Mol Biol. 2010 Jun 11;399(3):501-11. doi: 10.1016/j.jmb.2010.04.029. Epub 2010 Apr 22.


The magnitude of the light-induced conformational change in different rhodopsins correlates with their ability to activate G proteins.

Tsukamoto H, Farrens DL, Koyanagi M, Terakita A.

J Biol Chem. 2009 Jul 31;284(31):20676-83. doi: 10.1074/jbc.M109.016212. Epub 2009 Jun 4.


Dynamics of arrestin-rhodopsin interactions: loop movement is involved in arrestin activation and receptor binding.

Sommer ME, Farrens DL, McDowell JH, Weber LA, Smith WC.

J Biol Chem. 2007 Aug 31;282(35):25560-8. Epub 2007 Jul 2.


The conformations of the manganese transport regulator of Bacillus subtilis in its metal-free state.

DeWitt MA, Kliegman JI, Helmann JD, Brennan RG, Farrens DL, Glasfeld A.

J Mol Biol. 2007 Feb 2;365(5):1257-65. Epub 2006 Oct 28.


Arrestin can act as a regulator of rhodopsin photochemistry.

Sommer ME, Farrens DL.

Vision Res. 2006 Dec;46(27):4532-46. Epub 2006 Oct 27.


Rhodopsin self-associates in asolectin liposomes.

Mansoor SE, Palczewski K, Farrens DL.

Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3060-5. Epub 2006 Feb 21.


Dynamics of arrestin-rhodopsin interactions: acidic phospholipids enable binding of arrestin to purified rhodopsin in detergent.

Sommer ME, Smith WC, Farrens DL.

J Biol Chem. 2006 Apr 7;281(14):9407-17. Epub 2006 Jan 20.


Dynamics of arrestin-rhodopsin interactions: arrestin and retinal release are directly linked events.

Sommer ME, Smith WC, Farrens DL.

J Biol Chem. 2005 Feb 25;280(8):6861-71. Epub 2004 Dec 9.


Role of the retinal hydrogen bond network in rhodopsin Schiff base stability and hydrolysis.

Janz JM, Farrens DL.

J Biol Chem. 2004 Dec 31;279(53):55886-94. Epub 2004 Oct 8.


Rhodopsin activation exposes a key hydrophobic binding site for the transducin alpha-subunit C terminus.

Janz JM, Farrens DL.

J Biol Chem. 2004 Jul 9;279(28):29767-73. Epub 2004 Apr 7.


Stability of dark state rhodopsin is mediated by a conserved ion pair in intradiscal loop E-2.

Janz JM, Fay JF, Farrens DL.

J Biol Chem. 2003 May 9;278(19):16982-91. Epub 2003 Jan 23.


Design, expression, and characterization of a synthetic human cannabinoid receptor and cannabinoid receptor/ G-protein fusion protein.

Farrens DL, Dunham TD, Fay JF, Dews IC, Caldwell J, Nauert B.

J Pept Res. 2002 Dec;60(6):336-47.


Engineering a functional blue-wavelength-shifted rhodopsin mutant.

Janz JM, Farrens DL.

Biochemistry. 2001 Jun 19;40(24):7219-27.


Agonist-induced conformational changes in the G-protein-coupling domain of the beta 2 adrenergic receptor.

Ghanouni P, Steenhuis JJ, Farrens DL, Kobilka BK.

Proc Natl Acad Sci U S A. 2001 May 22;98(11):5997-6002. Epub 2001 May 15.


Functionally different agonists induce distinct conformations in the G protein coupling domain of the beta 2 adrenergic receptor.

Ghanouni P, Gryczynski Z, Steenhuis JJ, Lee TW, Farrens DL, Lakowicz JR, Kobilka BK.

J Biol Chem. 2001 Jul 6;276(27):24433-6. Epub 2001 Apr 24.


Consensus and variant cAMP-regulated enhancers have distinct CREB-binding properties.

Craig JC, Schumacher MA, Mansoor SE, Farrens DL, Brennan RG, Goodman RH.

J Biol Chem. 2001 Apr 13;276(15):11719-28. Epub 2000 Dec 27.


Domains responsible for constitutive and Ca(2+)-dependent interactions between calmodulin and small conductance Ca(2+)-activated potassium channels.

Keen JE, Khawaled R, Farrens DL, Neelands T, Rivard A, Bond CT, Janowsky A, Fakler B, Adelman JP, Maylie J.

J Neurosci. 1999 Oct 15;19(20):8830-8.


Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin.

Farrens DL, Altenbach C, Yang K, Hubbell WL, Khorana HG.

Science. 1996 Nov 1;274(5288):768-70.


Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: a site-directed spin-labeling study.

Altenbach C, Yang K, Farrens DL, Farahbakhsh ZT, Khorana HG, Hubbell WL.

Biochemistry. 1996 Sep 24;35(38):12470-8.


Hydrophobic amino acids in the retinal-binding pocket of bacteriorhodopsin.

Greenhalgh DA, Farrens DL, Subramaniam S, Khorana HG.

J Biol Chem. 1993 Sep 25;268(27):20305-11.

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