Format
Sort by
Items per page

Send to

Choose Destination

Search results

Items: 1 to 50 of 59

1.

Basic Concepts and Validation of Digital PCR Measurements.

Pinheiro L, Emslie KR.

Methods Mol Biol. 2018;1768:11-24. doi: 10.1007/978-1-4939-7778-9_2.

PMID:
29717435
2.

Interlaboratory Reproducibility of Droplet Digital Polymerase Chain Reaction Using a New DNA Reference Material Format.

Pinheiro LB, O'Brien H, Druce J, Do H, Kay P, Daniels M, You J, Burke D, Griffiths K, Emslie KR.

Anal Chem. 2017 Nov 7;89(21):11243-11251. doi: 10.1021/acs.analchem.6b05032. Epub 2017 Oct 18.

PMID:
28968098
3.

International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.

Yoo HB, Park SR, Dong L, Wang J, Sui Z, Pavšič J, Milavec M, Akgoz M, Mozioğlu E, Corbisier P, Janka M, Cosme B, de V Cavalcante JJ, Flatshart RB, Burke D, Forbes-Smith M, McLaughlin J, Emslie K, Whale AS, Huggett JF, Parkes H, Kline MC, Harenza JL, Vallone PM.

Anal Chem. 2016 Dec 20;88(24):12169-12176. doi: 10.1021/acs.analchem.6b03076. Epub 2016 Nov 30.

PMID:
28193036
4.

Digital polymerase chain reaction for characterisation of DNA reference materials.

Bhat S, Emslie KR.

Biomol Detect Quantif. 2016 May 3;10:47-49. eCollection 2016 Dec. Review.

5.

Synthetic certified DNA reference material for analysis of human erythropoietin transgene and transcript in gene doping and gene therapy.

Baoutina A, Bhat S, Zheng M, Partis L, Dobeson M, Alexander IE, Emslie KR.

Gene Ther. 2016 Oct;23(10):708-717. doi: 10.1038/gt.2016.47. Epub 2016 Jun 7.

PMID:
27439362
6.

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

Devonshire AS, Sanders R, Whale AS, Nixon GJ, Cowen S, Ellison SL, Parkes H, Pine PS, Salit M, McDaniel J, Munro S, Lund S, Matsukura S, Sekiguchi Y, Kawaharasaki M, Granjeiro JM, Falagan-Lotsch P, Saraiva AM, Couto P, Yang I, Kwon H, Park SR, Demšar T, Žel J, Blejec A, Milavec M, Dong L, Zhang L, Sui Z, Wang J, Viroonudomphol D, Prawettongsopon C, Partis L, Baoutina A, Emslie K, Takatsu A, Akyurek S, Akgoz M, Vonsky M, Konopelko LA, Cundapi EM, Urquiza MP, Huggett JF, Foy CA.

Biomol Detect Quantif. 2016 Jun 6;8:15-28. doi: 10.1016/j.bdq.2016.05.003. eCollection 2016 Jun.

7.

DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials.

Corbisier P, Pinheiro L, Mazoua S, Kortekaas AM, Chung PY, Gerganova T, Roebben G, Emons H, Emslie K.

Anal Bioanal Chem. 2015 Mar;407(7):1831-40. doi: 10.1007/s00216-015-8458-z. Epub 2015 Jan 20.

8.

Measurement of absolute copy number variation reveals association with essential hypertension.

Marques FZ, Prestes PR, Pinheiro LB, Scurrah K, Emslie KR, Tomaszewski M, Harrap SB, Charchar FJ.

BMC Med Genomics. 2014 Jul 15;7:44. doi: 10.1186/1755-8794-7-44.

9.

DNA methylation ratio variability may impede clinical application of cancer diagnostic markers.

Burke D, Fu S, Forbes-Smith M, Bhat S, Partis L, Coldham T, Frenkel R, Emslie KR.

Anal Bioanal Chem. 2014 Oct;406(26):6529-37. doi: 10.1007/s00216-014-7914-5. Epub 2014 Jun 20.

PMID:
24948089
10.

Validation of DNA methylation biomarkers for diagnosis of acute lymphoblastic leukemia.

Chatterton Z, Burke D, Emslie KR, Craig JM, Ng J, Ashley DM, Mechinaud F, Saffery R, Wong NC.

Clin Chem. 2014 Jul;60(7):995-1003. doi: 10.1373/clinchem.2013.219956. Epub 2014 May 14.

11.

Improved detection of transgene and nonviral vectors in blood.

Baoutina A, Coldham T, Fuller B, Emslie KR.

Hum Gene Ther Methods. 2013 Dec;24(6):345-54. doi: 10.1089/hgtb.2013.128. Epub 2013 Sep 26.

PMID:
23971678
12.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

13.

Digital polymerase chain reaction measured pUC19 marker as calibrant for HPLC measurement of DNA quantity.

Burke DG, Dong L, Bhat S, Forbes-Smith M, Fu S, Pinheiro L, Jing W, Emslie KR.

Anal Chem. 2013 Feb 5;85(3):1657-64. doi: 10.1021/ac302925f. Epub 2013 Jan 8.

PMID:
23215355
14.

The effect of input DNA copy number on genotype call and characterising SNP markers in the humpback whale genome using a nanofluidic array.

Bhat S, Polanowski AM, Double MC, Jarman SN, Emslie KR.

PLoS One. 2012;7(6):e39181. doi: 10.1371/journal.pone.0039181. Epub 2012 Jun 20.

15.

Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification.

Pinheiro LB, Coleman VA, Hindson CM, Herrmann J, Hindson BJ, Bhat S, Emslie KR.

Anal Chem. 2012 Jan 17;84(2):1003-11. doi: 10.1021/ac202578x. Epub 2011 Dec 21.

16.

A standard additions method reduces inhibitor-induced bias in quantitative real-time PCR.

Ellison SL, Emslie KR, Kassir Z.

Anal Bioanal Chem. 2011 Dec;401(10):3221-7. doi: 10.1007/s00216-011-5460-y. Epub 2011 Oct 15.

PMID:
22002559
17.

Effect of sustained elevated temperature prior to amplification on template copy number estimation using digital polymerase chain reaction.

Bhat S, McLaughlin JL, Emslie KR.

Analyst. 2011 Feb 21;136(4):724-32. doi: 10.1039/c0an00484g. Epub 2010 Nov 25.

PMID:
21107463
18.

Comparison of methods for accurate quantification of DNA mass concentration with traceability to the international system of units.

Bhat S, Curach N, Mostyn T, Bains GS, Griffiths KR, Emslie KR.

Anal Chem. 2010 Sep 1;82(17):7185-92. doi: 10.1021/ac100845m.

PMID:
20690645
19.

Gene doping detection: evaluation of approach for direct detection of gene transfer using erythropoietin as a model system.

Baoutina A, Coldham T, Bains GS, Emslie KR.

Gene Ther. 2010 Aug;17(8):1022-32. doi: 10.1038/gt.2010.49. Epub 2010 May 13.

PMID:
20463760
20.

Detection and identification of multiple genetically modified events using DNA insert fingerprinting.

Raymond P, Gendron L, Khalf M, Paul S, Dibley KL, Bhat S, Xie VR, Partis L, Moreau ME, Dollard C, Coté MJ, Laberge S, Emslie KR.

Anal Bioanal Chem. 2010 Mar;396(6):2091-102. doi: 10.1007/s00216-009-3295-6. Epub 2009 Nov 27.

PMID:
19943159
21.

Absolute quantification of genetically modified MON810 maize (Zea mays L.) by digital polymerase chain reaction.

Corbisier P, Bhat S, Partis L, Xie VR, Emslie KR.

Anal Bioanal Chem. 2010 Mar;396(6):2143-50. doi: 10.1007/s00216-009-3200-3. Epub 2009 Oct 9.

PMID:
19816678
22.

Accurate measurement of DNA methylation that is traceable to the international system of units.

Burke DG, Griffiths K, Kassir Z, Emslie K.

Anal Chem. 2009 Sep 1;81(17):7294-301. doi: 10.1021/ac901116f.

PMID:
19715360
23.

Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number.

Bhat S, Herrmann J, Armishaw P, Corbisier P, Emslie KR.

Anal Bioanal Chem. 2009 May;394(2):457-67. doi: 10.1007/s00216-009-2729-5. Epub 2009 Mar 15.

PMID:
19288230
24.

An international comparability study on quantification of total methyl cytosine content.

Yang I, Kim SK, Burke DG, Griffiths K, Kassir Z, Emslie KR, Gao Y, Wang J, Foy CA, Pardos-Pardos AC, Ellison S, Domann PJ, Fujii S, Park SR.

Anal Biochem. 2009 Jan 15;384(2):288-95. doi: 10.1016/j.ab.2008.09.036. Epub 2008 Sep 30.

PMID:
18929528
25.

Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence.

Haber AL, Griffiths KR, Jamting AK, Emslie KR.

Anal Bioanal Chem. 2008 Nov;392(5):887-96. doi: 10.1007/s00216-008-2358-4. Epub 2008 Sep 14.

PMID:
18791860
26.

Developing strategies for detection of gene doping.

Baoutina A, Alexander IE, Rasko JE, Emslie KR.

J Gene Med. 2008 Jan;10(1):3-20. Review.

PMID:
18081214
27.

A study of comparability in amplified fragment length polymorphism profiling using a simple model system.

Partis L, Burns M, Chiba K, Corbisier P, Gancberg D, Holden MJ, Wang J, Liu QY, Okunishi T, Yang I, Vonsky M, Emslie KR.

Electrophoresis. 2007 Sep;28(18):3193-200.

PMID:
17696213
28.

Potential use of gene transfer in athletic performance enhancement.

Baoutina A, Alexander IE, Rasko JE, Emslie KR.

Mol Ther. 2007 Oct;15(10):1751-66. Epub 2007 Aug 7. Review.

29.

Sampling plan and test protocol for the semiquantitative detection of genetically modified canola (Brassica napus) seed in bulk canola seed.

Emslie KR, Whaites L, Griffiths KR, Murby EJ.

J Agric Food Chem. 2007 May 30;55(11):4414-21. Epub 2007 May 8.

PMID:
17488033
30.

Toward metrological traceability for DNA fragment ratios in GM quantification. 1. Effect of DNA extraction methods on the quantitative determination of Bt176 corn by real-time PCR.

Corbisier P, Broothaerts W, Gioria S, Schimmel H, Burns M, Baoutina A, Emslie KR, Furui S, Kurosawa Y, Holden MJ, Kim HH, Lee YM, Kawaharasaki M, Sin D, Wang J.

J Agric Food Chem. 2007 May 2;55(9):3249-57. Epub 2007 Apr 4.

PMID:
17407305
31.

Second-generation blood tests to detect erythropoietin abuse by athletes.

Gore CJ, Parisotto R, Ashenden MJ, Stray-Gundersen J, Sharpe K, Hopkins W, Emslie KR, Howe C, Trout GJ, Kazlauskas R, Hahn AG.

Haematologica. 2003 Mar;88(3):333-44.

32.

Development of reference ranges in elite athletes for markers of altered erythropoiesis.

Sharpe K, Hopkins W, Emslie KR, Howe C, Trout GJ, Kazlauskas R, Ashenden MJ, Gore CJ, Parisotto R, Hahn AG.

Haematologica. 2002 Dec;87(12):1248-57.

33.

Serotype classification and characterisation of the rotavirus SA11 VP6 protein using mass spectrometry and two-dimensional gel electrophoresis.

Emslie KR, Molloy MP, Barardi CR, Jardine D, Wilkins MR, Bellamy AR, Williams KL.

Funct Integr Genomics. 2000 May;1(1):12-24.

PMID:
11793218
34.

Detection of recombinant human erythropoietin abuse in athletes utilizing markers of altered erythropoiesis.

Parisotto R, Wu M, Ashenden MJ, Emslie KR, Gore CJ, Howe C, Kazlauskas R, Sharpe K, Trout GJ, Xie M.

Haematologica. 2001 Feb;86(2):128-37.

35.

A novel method utilising markers of altered erythropoiesis for the detection of recombinant human erythropoietin abuse in athletes.

Parisotto R, Gore CJ, Emslie KR, Ashenden MJ, Brugnara C, Howe C, Martin DT, Trout GJ, Hahn AG.

Haematologica. 2000 Jun;85(6):564-72.

36.

Simulated moderate altitude elevates serum erythropoietin but does not increase reticulocyte production in well-trained runners.

Ashenden MJ, Gore CJ, Dobson GP, Boston TT, Parisotto R, Emslie KR, Trout GJ, Hahn AG.

Eur J Appl Physiol. 2000 Mar;81(5):428-35.

PMID:
10751105
37.

Development of a rapid and sensitive quantitative assay for rotavirus based on flow cytometry.

Barardi CR, Emslie KR, Vesey G, Williams KL.

J Virol Methods. 1998 Sep;74(1):31-8.

PMID:
9763126
38.

Expression of recombinant glycoproteins in the simple eukaryote Dictyostelium discoideum.

Slade MB, Emslie KR, Williams KL.

Biotechnol Genet Eng Rev. 1997;14:1-35. Review. No abstract available.

PMID:
9188148
39.
40.

Calcium influences the stability and conformation of rotavirus SA11 glycoprotein VP7 expressed in Dictyostelium discoideum.

Emslie KR, Coukell MB, Birch D, Williams KL.

J Biotechnol. 1996 Oct 1;50(2-3):149-59.

PMID:
8987623
41.

From virus to vaccine: developments using the simple eukaryote, Dictyostelium discoideum.

Emslie KR, Slade MB, Williams KL.

Trends Microbiol. 1995 Dec;3(12):476-9. Review.

PMID:
8800841
42.

Recombinant glycoprotein production in the slime mould Dictyostelium discoideum.

Williams KL, Emslie KR, Slade MB.

Curr Opin Biotechnol. 1995 Oct;6(5):538-42. Review.

PMID:
7579665
43.

Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum.

Emslie KR, Miller JM, Slade MB, Dormitzer PR, Greenberg HB, Williams KL.

J Virol. 1995 Mar;69(3):1747-54.

44.
45.
46.

Transphyseal blood vessels exist in avian species.

Alderson M, Emslie K, Speers D, Nade S.

J Anat. 1986 Jun;146:217-24.

47.
48.
49.

A resistance determinant to nucleic acid-binding compounds in methicillin-resistant Staphylococcus aureus.

Emslie KR, Townsend DE, Grubb WB.

J Med Microbiol. 1985 Oct;20(2):139-45.

PMID:
3850128
50.

Supplemental Content

Loading ...
Support Center