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Science. 2016 Jul 8;353(6295):aad9969. doi: 10.1126/science.aad9969. Epub 2016 May 19.

Asymmetric division of clonal muscle stem cells coordinates muscle regeneration in vivo.

Author information

1
Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
2
School of Biological Sciences, Building 18, Monash University, Clayton, Victoria 3800, Australia.
3
Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia. European Molecular Biology Laboratory Australia Melbourne Node, Level 1, Building 75, Monash University, Wellington Road, Clayton, Victoria 3800, Australia. peter.currie@monash.edu.

Abstract

Skeletal muscle is an example of a tissue that deploys a self-renewing stem cell, the satellite cell, to effect regeneration. Recent in vitro studies have highlighted a role for asymmetric divisions in renewing rare "immortal" stem cells and generating a clonal population of differentiation-competent myoblasts. However, this model currently lacks in vivo validation. We define a zebrafish muscle stem cell population analogous to the mammalian satellite cell and image the entire process of muscle regeneration from injury to fiber replacement in vivo. This analysis reveals complex interactions between satellite cells and both injured and uninjured fibers and provides in vivo evidence for the asymmetric division of satellite cells driving both self-renewal and regeneration via a clonally restricted progenitor pool.

PMID:
27198673
DOI:
10.1126/science.aad9969
[Indexed for MEDLINE]
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