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Towards the structural characterization of the human methyltransferome.

Fenwick MK, Ealick SE.

Curr Opin Struct Biol. 2018 Apr 11;53:12-21. doi: 10.1016/ [Epub ahead of print] Review.


Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis.

Dong M, Kathiresan V, Fenwick MK, Torelli AT, Zhang Y, Caranto JD, Dzikovski B, Sharma A, Lancaster KM, Freed JH, Ealick SE, Hoffman BM, Lin H.

Science. 2018 Mar 16;359(6381):1247-1250. doi: 10.1126/science.aao6595.


Biochemical Characterization and Structural Basis of Reactivity and Regioselectivity Differences between Burkholderia thailandensis and Burkholderia glumae 1,6-Didesmethyltoxoflavin N-Methyltransferase.

Fenwick MK, Almabruk KH, Ealick SE, Begley TP, Philmus B.

Biochemistry. 2017 Aug 1;56(30):3934-3944. doi: 10.1021/acs.biochem.7b00476. Epub 2017 Jul 20.


Structural studies of viperin, an antiviral radical SAM enzyme.

Fenwick MK, Li Y, Cresswell P, Modis Y, Ealick SE.

Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):6806-6811. doi: 10.1073/pnas.1705402114. Epub 2017 Jun 12.


Substrate-Dependent Cleavage Site Selection by Unconventional Radical S-Adenosylmethionine Enzymes in Diphthamide Biosynthesis.

Dong M, Horitani M, Dzikovski B, Freed JH, Ealick SE, Hoffman BM, Lin H.

J Am Chem Soc. 2017 Apr 26;139(16):5680-5683. doi: 10.1021/jacs.7b01712. Epub 2017 Apr 13.


Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis.

Rodrigues MJ, Windeisen V, Zhang Y, Guédez G, Weber S, Strohmeier M, Hanes JW, Royant A, Evans G, Sinning I, Ealick SE, Begley TP, Tews I.

Nat Chem Biol. 2017 Mar;13(3):290-294. doi: 10.1038/nchembio.2273. Epub 2017 Jan 16.


Lyme disease spirochaete Borrelia burgdorferi does not require thiamin.

Zhang K, Bian J, Deng Y, Smith A, Nunez RE, Li MB, Pal U, Yu AM, Qiu W, Ealick SE, Li C.

Nat Microbiol. 2016 Nov 21;2:16213. doi: 10.1038/nmicrobiol.2016.213.


Crystal Structures of the Iron-Sulfur Cluster-Dependent Quinolinate Synthase in Complex with Dihydroxyacetone Phosphate, Iminoaspartate Analogues, and Quinolinate.

Fenwick MK, Ealick SE.

Biochemistry. 2016 Aug 2;55(30):4135-9. doi: 10.1021/acs.biochem.6b00626. Epub 2016 Jul 22.


Biochemical and structural characterization of Klebsiella pneumoniae oxamate amidohydrolase in the uric acid degradation pathway.

Hicks KA, Ealick SE.

Acta Crystallogr D Struct Biol. 2016 Jun;72(Pt 6):808-16. doi: 10.1107/S2059798316007099. Epub 2016 May 25.


Burkholderia glumae ToxA Is a Dual-Specificity Methyltransferase That Catalyzes the Last Two Steps of Toxoflavin Biosynthesis.

Fenwick MK, Philmus B, Begley TP, Ealick SE.

Biochemistry. 2016 May 17;55(19):2748-59. doi: 10.1021/acs.biochem.6b00167. Epub 2016 May 3.


From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.

Eser BE, Zhang X, Chanani PK, Begley TP, Ealick SE.

J Am Chem Soc. 2016 Mar 23;138(11):3639-42. doi: 10.1021/jacs.6b00445. Epub 2016 Mar 11.


Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.

Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE.

Biochemistry. 2016 Mar 29;55(12):1826-38. doi: 10.1021/acs.biochem.6b00030. Epub 2016 Mar 10.


Polyketide Ring Expansion Mediated by a Thioesterase, Chain Elongation and Cyclization Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG.

Mori S, Simkhada D, Zhang H, Erb MS, Zhang Y, Williams H, Fedoseyenko D, Russell WK, Kim D, Fleer N, Ealick SE, Watanabe CM.

Biochemistry. 2016 Feb 2;55(4):704-14. doi: 10.1021/acs.biochem.5b01050. Epub 2016 Jan 19.


Anaerobic 5-Hydroxybenzimidazole Formation from Aminoimidazole Ribotide: An Unanticipated Intersection of Thiamin and Vitamin B₁₂ Biosynthesis.

Mehta AP, Abdelwahed SH, Fenwick MK, Hazra AB, Taga ME, Zhang Y, Ealick SE, Begley TP.

J Am Chem Soc. 2015 Aug 26;137(33):10444-7. doi: 10.1021/jacs.5b03576. Epub 2015 Aug 13.


Different polyamine pathways from bacteria have replaced eukaryotic spermidine biosynthesis in ciliates Tetrahymena thermophila and Paramecium tetaurelia.

Li B, Kim SH, Zhang Y, Hanfrey CC, Elliott KA, Ealick SE, Michael AJ.

Mol Microbiol. 2015 Sep;97(5):791-807. doi: 10.1111/mmi.13066. Epub 2015 Jun 8.


Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase.

Fenwick MK, Mehta AP, Zhang Y, Abdelwahed SH, Begley TP, Ealick SE.

Nat Commun. 2015 Mar 27;6:6480. doi: 10.1038/ncomms7480.


Ethenoguanines undergo glycosylation by nucleoside 2'-deoxyribosyltransferases at non-natural sites.

Ye W, Paul D, Gao L, Seckute J, Sangaiah R, Jayaraj K, Zhang Z, Kaminski PA, Ealick SE, Gold A, Ball LM.

PLoS One. 2014 Dec 18;9(12):e115082. doi: 10.1371/journal.pone.0115082. eCollection 2014.


Radical S-adenosylmethionine (SAM) enzymes in cofactor biosynthesis: a treasure trove of complex organic radical rearrangement reactions.

Mehta AP, Abdelwahed SH, Mahanta N, Fedoseyenko D, Philmus B, Cooper LE, Liu Y, Jhulki I, Ealick SE, Begley TP.

J Biol Chem. 2015 Feb 13;290(7):3980-6. doi: 10.1074/jbc.R114.623793. Epub 2014 Dec 4. Review.


Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis.

Sasaki E, Zhang X, Sun HG, Lu MY, Liu TL, Ou A, Li JY, Chen YH, Ealick SE, Liu HW.

Nature. 2014 Jun 19;510(7505):427-31. doi: 10.1038/nature13256. Epub 2014 May 11.


Structure of a Clostridium botulinum C143S thiaminase I/thiamin complex reveals active site architecture .

Sikowitz MD, Shome B, Zhang Y, Begley TP, Ealick SE.

Biochemistry. 2013 Nov 5;52(44):7830-9. doi: 10.1021/bi400841g. Epub 2013 Oct 25.


Active-site models for complexes of quinolinate synthase with substrates and intermediates.

Soriano EV, Zhang Y, Colabroy KL, Sanders JM, Settembre EC, Dorrestein PC, Begley TP, Ealick SE.

Acta Crystallogr D Biol Crystallogr. 2013 Sep;69(Pt 9):1685-96. doi: 10.1107/S090744491301247X. Epub 2013 Aug 15.


Reversal of the substrate specificity of CMP N-glycosidase to dCMP.

Sikowitz MD, Cooper LE, Begley TP, Kaminski PA, Ealick SE.

Biochemistry. 2013 Jun 11;52(23):4037-47. doi: 10.1021/bi400316p. Epub 2013 May 28.


Structural and mechanistic studies of HpxO, a novel flavin adenine dinucleotide-dependent urate oxidase from Klebsiella pneumoniae.

Hicks KA, O'Leary SE, Begley TP, Ealick SE.

Biochemistry. 2013 Jan 22;52(3):477-87. doi: 10.1021/bi301262p. Epub 2013 Jan 9.


Pseudouridine monophosphate glycosidase: a new glycosidase mechanism.

Huang S, Mahanta N, Begley TP, Ealick SE.

Biochemistry. 2012 Nov 13;51(45):9245-55. doi: 10.1021/bi3006829. Epub 2012 Oct 30.


Reverse structural genomics: an unusual flavin-binding site in a putative protease from Bacteroides thetaiotaomicron.

Knaus T, Eger E, Koop J, Stipsits S, Kinsland CL, Ealick SE, Macheroux P.

J Biol Chem. 2012 Aug 10;287(33):27490-8. doi: 10.1074/jbc.M112.355388. Epub 2012 Jun 20.


Thiamin biosynthesis: still yielding fascinating biological chemistry.

Begley TP, Ealick SE, McLafferty FW.

Biochem Soc Trans. 2012 Jun 1;40(3):555-60. doi: 10.1042/BST20120084. Review.


Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate.

Lai RY, Huang S, Fenwick MK, Hazra A, Zhang Y, Rajashankar K, Philmus B, Kinsland C, Sanders JM, Ealick SE, Begley TP.

J Am Chem Soc. 2012 Jun 6;134(22):9157-9. doi: 10.1021/ja302474a. Epub 2012 May 24.


A corrected space group for Sulfolobus sulfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II.

Zhang Y, Zwart PH, Ealick SE.

Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):249-52. doi: 10.1107/S0907444911051699. Epub 2012 Feb 7.


Structural basis of the substrate specificity of Bacillus cereus adenosine phosphorylase.

Dessanti P, Zhang Y, Allegrini S, Tozzi MG, Sgarrella F, Ealick SE.

Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):239-48. doi: 10.1107/S090744491200073X. Epub 2012 Feb 14.


Identification of the product of toxoflavin lyase: degradation via a Baeyer-Villiger oxidation.

Philmus B, Abdelwahed S, Williams HJ, Fenwick MK, Ealick SE, Begley TP.

J Am Chem Soc. 2012 Mar 21;134(11):5326-30. doi: 10.1021/ja211759n. Epub 2012 Mar 9.


Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.

Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP.

Nature. 2011 Oct 26;478(7370):542-6. doi: 10.1038/nature10503.


A novel mechanism of sulfur transfer catalyzed by O-acetylhomoserine sulfhydrylase in the methionine-biosynthetic pathway of Wolinella succinogenes.

Tran TH, Krishnamoorthy K, Begley TP, Ealick SE.

Acta Crystallogr D Biol Crystallogr. 2011 Oct;67(Pt 10):831-8. doi: 10.1107/S0907444911028010. Epub 2011 Sep 8.


Structure of trifunctional THI20 from yeast.

French JB, Begley TP, Ealick SE.

Acta Crystallogr D Biol Crystallogr. 2011 Sep;67(Pt 9):784-91. doi: 10.1107/S0907444911024814. Epub 2011 Aug 9.


Binding and inhibition of human spermidine synthase by decarboxylated S-adenosylhomocysteine.

Sečkutė J, McCloskey DE, Thomas HJ, Secrist JA 3rd, Pegg AE, Ealick SE.

Protein Sci. 2011 Nov;20(11):1836-44. doi: 10.1002/pro.717. Epub 2011 Sep 15.


Structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from Klebsiella pneumoniae.

French JB, Ealick SE.

Acta Crystallogr D Biol Crystallogr. 2011 Aug;67(Pt 8):671-7. doi: 10.1107/S090744491101746X. Epub 2011 Jul 12.


Pyridoxal phosphate: biosynthesis and catabolism.

Mukherjee T, Hanes J, Tews I, Ealick SE, Begley TP.

Biochim Biophys Acta. 2011 Nov;1814(11):1585-96. doi: 10.1016/j.bbapap.2011.06.018. Epub 2011 Jul 8. Review.


The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases.

Tran TH, Christoffersen S, Allan PW, Parker WB, Piskur J, Serra I, Terreni M, Ealick SE.

Biochemistry. 2011 Aug 2;50(30):6549-58. doi: 10.1021/bi200707z. Epub 2011 Jul 8.


Flavogenomics--a genomic and structural view of flavin-dependent proteins.

Macheroux P, Kappes B, Ealick SE.

FEBS J. 2011 Aug;278(15):2625-34. doi: 10.1111/j.1742-4658.2011.08202.x. Epub 2011 Jul 5. Review.


Characterization of the structure and function of Klebsiella pneumoniae allantoin racemase.

French JB, Neau DB, Ealick SE.

J Mol Biol. 2011 Jul 15;410(3):447-60. doi: 10.1016/j.jmb.2011.05.016. Epub 2011 May 17.


A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.

Hazra AB, Han Y, Chatterjee A, Zhang Y, Lai RY, Ealick SE, Begley TP.

J Am Chem Soc. 2011 Jun 22;133(24):9311-9. doi: 10.1021/ja1110514. Epub 2011 May 26.


The Leishmania donovani UMP synthase is essential for promastigote viability and has an unusual tetrameric structure that exhibits substrate-controlled oligomerization.

French JB, Yates PA, Soysa DR, Boitz JM, Carter NS, Chang B, Ullman B, Ealick SE.

J Biol Chem. 2011 Jun 10;286(23):20930-41. doi: 10.1074/jbc.M111.228213. Epub 2011 Apr 19.


Toxoflavin lyase requires a novel 1-His-2-carboxylate facial triad.

Fenwick MK, Philmus B, Begley TP, Ealick SE.

Biochemistry. 2011 Feb 15;50(6):1091-100. doi: 10.1021/bi101741v. Epub 2011 Jan 20.


Construction of a thiamin sensor from the periplasmic thiamin binding protein.

Hanes JW, Chatterjee D, Soriano EV, Ealick SE, Begley TP.

Chem Commun (Camb). 2011 Feb 28;47(8):2273-5. doi: 10.1039/c0cc04228e. Epub 2010 Dec 2.


A maize thiamine auxotroph is defective in shoot meristem maintenance.

Woodward JB, Abeydeera ND, Paul D, Phillips K, Rapala-Kozik M, Freeling M, Begley TP, Ealick SE, McSteen P, Scanlon MJ.

Plant Cell. 2010 Oct;22(10):3305-17. doi: 10.1105/tpc.110.077776. Epub 2010 Oct 22.


Domain organization in Candida glabrata THI6, a bifunctional enzyme required for thiamin biosynthesis in eukaryotes.

Paul D, Chatterjee A, Begley TP, Ealick SE.

Biochemistry. 2010 Nov 16;49(45):9922-34. doi: 10.1021/bi101008u. Epub 2010 Oct 22.


Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis.

Zhu X, Dzikovski B, Su X, Torelli AT, Zhang Y, Ealick SE, Freed JH, Lin H.

Mol Biosyst. 2011 Jan;7(1):74-81. doi: 10.1039/c0mb00076k. Epub 2010 Oct 8.


HMP binding protein ThiY and HMP-P synthase THI5 are structural homologues.

Bale S, Rajashankar KR, Perry K, Begley TP, Ealick SE.

Biochemistry. 2010 Oct 19;49(41):8929-36. doi: 10.1021/bi101209t.


Structural and mechanistic studies on Klebsiella pneumoniae 2-Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase.

French JB, Ealick SE.

J Biol Chem. 2010 Nov 12;285(46):35446-54. doi: 10.1074/jbc.M110.156034. Epub 2010 Sep 8.


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