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Biol Open. 2018 Feb 20;7(2). pii: bio029660. doi: 10.1242/bio.029660.

Spermine modulates fungal morphogenesis and activates plasma membrane H+-ATPase during yeast to hyphae transition.

Author information

1
Laboratório de Fisiologia e Bioquímica de Microrganismos, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Pq. Califórnia, Campos dos Goytacazes-RJ 28013-602, Brazil.
2
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Pq. Califórnia, Campos dos Goytacazes-RJ 28013-602, Brazil.
3
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Pq. Califórnia, Campos dos Goytacazes-RJ 28013-602, Brazil anna.uenf@gmail.com arnoldo@uenf.br.
4
Laboratório de Fisiologia e Bioquímica de Microrganismos, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Pq. Califórnia, Campos dos Goytacazes-RJ 28013-602, Brazil anna.uenf@gmail.com arnoldo@uenf.br.

Abstract

Polyamines play a regulatory role in eukaryotic cell growth and morphogenesis. Despite many molecular advances, the underlying mechanism of action remains unclear. Here, we investigate a mechanism by which spermine affects the morphogenesis of a dimorphic fungal model of emerging relevance in plant interactions, Yarrowia lipolytica, through the recruitment of a phytohormone-like pathway involving activation of the plasma membrane P-type H+-ATPase. Morphological transition was followed microscopically, and the H+-ATPase activity was analyzed in isolated membrane vesicles. Proton flux and acidification were directly probed at living cell surfaces by a non-invasive selective ion electrode technique. Spermine and indol-3-acetic acid (IAA) induced the yeast-hypha transition, influencing the colony architecture. Spermine induced H+-ATPase activity and H+ efflux in living cells correlating with yeast-hypha dynamics. Pharmacological inhibition of spermine and IAA pathways prevented the physio-morphological responses, and indicated that spermine could act upstream of the IAA pathway. This study provides the first compelling evidence on the fungal morphogenesis and colony development as modulated by a spermine-induced acid growth mechanism analogous to that previously postulated for the multicellular growth regulation of plants.

KEYWORDS:

Acid growth theory; H+ transport; P-type ATPase; Polarized growth; Polyamine; Scanning ion-selective electrode technique; Yarrowia lipolytica

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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