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Forensic Sci Int. 2014 Jul;240:41-7. doi: 10.1016/j.forsciint.2014.02.028. Epub 2014 Apr 3.

The environmental biological signature: NGS profiling for forensic comparison of soils.

Author information

1
University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy.
2
Carabinieri, Reparto Investigazioni Scientifiche di Roma, Viale di Tor di Quinto, 119, 00191 Roma, Italy.
3
Geoscienze Forensi Italia(®), Viale Mediterraneo 77, 00122 Rome, Italy.
4
Department of Biology, University of Florence, Via del Proconsolo 12, 50122 Florence, Italy.
5
Spygen, Savoie Technolac, Bâtiment Koala, 17, rue du Lac Saint-André, BP 274, 73375 Le Bourget-du-Lac Cedex, France.
6
University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy; MDD University Spin off, Viterbo, Italy. Electronic address: vincenzo.romanospica@uniroma4.it.

Abstract

The identification of the source of a specific soil sample is a crucial step in forensic investigations. Rapid advances in next generation sequencing (NGS) technology and the strong reduction of the cost of sequencing have recently opened new perspectives. In the present work a metabarcoding approach has been successfully applied to forensic and environmental soil samples, allowing the accurate and sensitive analysis of microflora (mfDNA), plants, metazoa, and protozoa DNA. The identification of the biological component by DNA metabarcoding is a strong element for the discrimination of samples geologically very similar but coming for distinct environments.

KEYWORDS:

Metabarcoding; Soil; mFDNA

PMID:
24807707
DOI:
10.1016/j.forsciint.2014.02.028
[Indexed for MEDLINE]

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