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Items: 1 to 50 of 62

1.

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.

De Zitter E, Thédié D, Mönkemöller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D.

Nat Methods. 2019 Aug;16(8):707-710. doi: 10.1038/s41592-019-0462-3. Epub 2019 Jul 8.

PMID:
31285624
2.

Optimizing the fluorescent protein toolbox and its use.

Duwé S, Dedecker P.

Curr Opin Biotechnol. 2019 Jun 3;58:183-191. doi: 10.1016/j.copbio.2019.04.006. [Epub ahead of print] Review.

PMID:
31170610
3.

Sensitive and specific detection of E. coli using biomimetic receptors in combination with a modified heat-transfer method.

Cornelis P, Givanoudi S, Yongabi D, Iken H, Duwé S, Deschaume O, Robbens J, Dedecker P, Bartic C, Wübbenhorst M, Schöning MJ, Heyndrickx M, Wagner P.

Biosens Bioelectron. 2019 Jul 1;136:97-105. doi: 10.1016/j.bios.2019.04.026. Epub 2019 Apr 24.

PMID:
31048213
4.

The Persistence-Inducing Toxin HokB Forms Dynamic Pores That Cause ATP Leakage.

Wilmaerts D, Bayoumi M, Dewachter L, Knapen W, Mika JT, Hofkens J, Dedecker P, Maglia G, Verstraeten N, Michiels J.

MBio. 2018 Aug 14;9(4). pii: e00744-18. doi: 10.1128/mBio.00744-18.

5.

Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI.

Hertel F, Mo GCH, Dedecker P, Zhang J.

Methods Mol Biol. 2018;1764:267-277. doi: 10.1007/978-1-4939-7759-8_16.

PMID:
29605920
6.

An introduction to optical super-resolution microscopy for the adventurous biologist.

Vangindertael J, Camacho R, Sempels W, Mizuno H, Dedecker P, Janssen KPF.

Methods Appl Fluoresc. 2018 Mar 16;6(2):022003. doi: 10.1088/2050-6120/aaae0c. Review.

PMID:
29422456
7.

Super-resolution imaging goes fast and deep.

Duwé S, Dedecker P.

Nat Methods. 2017 Oct 31;14(11):1042-1044. doi: 10.1038/nmeth.4484. No abstract available.

PMID:
29088130
8.

Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization.

Roebroek T, Duwé S, Vandenberg W, Dedecker P.

Int J Mol Sci. 2017 Sep 20;18(9). pii: E2015. doi: 10.3390/ijms18092015.

9.

Correcting for photodestruction in super-resolution optical fluctuation imaging.

Peeters Y, Vandenberg W, Duwé S, Bouwens A, Lukeš T, Ruckebusch C, Lasser T, Dedecker P.

Sci Rep. 2017 Sep 5;7(1):10470. doi: 10.1038/s41598-017-09666-4.

10.

Efficient switching of mCherry fluorescence using chemical caging.

Cloin BMC, De Zitter E, Salas D, Gielen V, Folkers GE, Mikhaylova M, Bergeler M, Krajnik B, Harvey J, Hoogenraad CC, Van Meervelt L, Dedecker P, Kapitein LC.

Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7013-7018. doi: 10.1073/pnas.1617280114. Epub 2017 Jun 19.

11.

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI.

Duwé S, Vandenberg W, Dedecker P.

Chem Commun (Camb). 2017 Jun 29;53(53):7242-7245. doi: 10.1039/c7cc02344h.

PMID:
28561832
12.

Effect of probe diffusion on the SOFI imaging accuracy.

Vandenberg W, Dedecker P.

Sci Rep. 2017 Mar 23;7:44665. doi: 10.1038/srep44665.

13.

Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution.

Mo GC, Ross B, Hertel F, Manna P, Yang X, Greenwald E, Booth C, Plummer AM, Tenner B, Chen Z, Wang Y, Kennedy EJ, Cole PA, Fleming KG, Palmer A, Jimenez R, Xiao J, Dedecker P, Zhang J.

Nat Methods. 2017 Apr;14(4):427-434. doi: 10.1038/nmeth.4221. Epub 2017 Mar 13.

14.

Field-Controlled Charge Separation in a Conductive Matrix at the Single-Molecule Level: Toward Controlling Single-Molecule Fluorescence Intermittency.

Kennes K, Dedecker P, Hutchison JA, Fron E, Uji-I H, Hofkens J, Van der Auweraer M.

ACS Omega. 2016 Dec 31;1(6):1383-1392. doi: 10.1021/acsomega.6b00207. Epub 2016 Dec 27.

15.

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions.

Deschout H, Lukes T, Sharipov A, Szlag D, Feletti L, Vandenberg W, Dedecker P, Hofkens J, Leutenegger M, Lasser T, Radenovic A.

Nat Commun. 2016 Dec 19;7:13693. doi: 10.1038/ncomms13693.

16.

SOFI Simulation Tool: A Software Package for Simulating and Testing Super-Resolution Optical Fluctuation Imaging.

Girsault A, Lukes T, Sharipov A, Geissbuehler S, Leutenegger M, Vandenberg W, Dedecker P, Hofkens J, Lasser T.

PLoS One. 2016 Sep 1;11(9):e0161602. doi: 10.1371/journal.pone.0161602. eCollection 2016.

17.

Corrigendum: Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

Vangindertael J, Beets I, Rocha S, Dedecker P, Schoofs L, Vanhoorelbeke K, Hofkens J, Mizuno H.

Sci Rep. 2016 May 20;7:26047. doi: 10.1038/srep26047. No abstract available.

18.

Corrigendum: Sparse deconvolution of high-density super-resolution images.

Hugelier S, de Rooi JJ, Bernex R, Duwé S, Devos O, Sliwa M, Dedecker P, Eilers PH, Ruckebusch C.

Sci Rep. 2016 May 9;6:24975. doi: 10.1038/srep24975. No abstract available.

19.

Model-free uncertainty estimation in stochastical optical fluctuation imaging (SOFI) leads to a doubled temporal resolution.

Vandenberg W, Duwé S, Leutenegger M, Moeyaert B, Krajnik B, Lasser T, Dedecker P.

Biomed Opt Express. 2016 Jan 15;7(2):467-80. doi: 10.1364/BOE.7.000467. eCollection 2016 Feb 1.

20.

Sparse deconvolution of high-density super-resolution images.

Hugelier S, de Rooi JJ, Bernex R, Duwé S, Devos O, Sliwa M, Dedecker P, Eilers PH, Ruckebusch C.

Sci Rep. 2016 Feb 25;6:21413. doi: 10.1038/srep21413. Erratum in: Sci Rep. 2016 May 09;6:24975.

21.

RefSOFI for Mapping Nanoscale Organization of Protein-Protein Interactions in Living Cells.

Hertel F, Mo GC, Duwé S, Dedecker P, Zhang J.

Cell Rep. 2016 Jan 12;14(2):390-400. doi: 10.1016/j.celrep.2015.12.036. Epub 2015 Dec 31.

22.

A study of SeqA subcellular localization in Escherichia coli using photo-activated localization microscopy.

Mika JT, Vanhecke A, Dedecker P, Swings T, Vangindertael J, Van den Bergh B, Michiels J, Hofkens J.

Faraday Discuss. 2015;184:425-50. doi: 10.1039/c5fd00058k. Epub 2015 Oct 9.

PMID:
26449690
23.

Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM.

Vangindertael J, Beets I, Rocha S, Dedecker P, Schoofs L, Vanhoorelbeke K, Hofkens J, Mizuno H.

Sci Rep. 2015 Sep 1;5:13532. doi: 10.1038/srep13532. Erratum in: Sci Rep. 2016;7:26047. Vanhoorelbeeke, Karen [corrected to Vanhoorelbeke, Karen].

24.

Expression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy.

Duwé S, De Zitter E, Gielen V, Moeyaert B, Vandenberg W, Grotjohann T, Clays K, Jakobs S, Van Meervelt L, Dedecker P.

ACS Nano. 2015 Oct 27;9(10):9528-41. doi: 10.1021/acsnano.5b04129. Epub 2015 Sep 9.

PMID:
26308583
25.

Molecular Dynamic Indicators of the Photoswitching Properties of Green Fluorescent Proteins.

Smyrnova D, Moeyaert B, Michielssens S, Hofkens J, Dedecker P, Ceulemans A.

J Phys Chem B. 2015 Sep 10;119(36):12007-16. doi: 10.1021/acs.jpcb.5b04826. Epub 2015 Aug 31.

PMID:
26305506
26.

Mapping pixel dissimilarity in wide-field super-resolution fluorescence microscopy.

Ruckebusch C, Bernex R, Allegrini F, Sliwa M, Hofkens J, Dedecker P.

Anal Chem. 2015 May 5;87(9):4675-82. doi: 10.1021/ac504295p. Epub 2015 Apr 17.

PMID:
25844921
27.

Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells.

Antoku Y, Dedecker P, Pinheiro PS, Vosch T, Sørensen JB.

Photochem Photobiol Sci. 2015 May;14(5):1005-12. doi: 10.1039/c4pp00423j.

PMID:
25837695
28.

Diffraction-unlimited fluorescence microscopy of living biological samples using pcSOFI.

Duwé S, Moeyaert B, Dedecker P.

Curr Protoc Chem Biol. 2015 Mar 2;7(1):27-41. doi: 10.1002/9780470559277.ch140025.

PMID:
25727061
29.

Diffraction-unlimited imaging: from pretty pictures to hard numbers.

Vandenberg W, Leutenegger M, Lasser T, Hofkens J, Dedecker P.

Cell Tissue Res. 2015 Apr;360(1):151-78. doi: 10.1007/s00441-014-2109-0. Epub 2015 Feb 28. Review.

PMID:
25722085
30.

Combing of genomic DNA from droplets containing picograms of material.

Deen J, Sempels W, De Dier R, Vermant J, Dedecker P, Hofkens J, Neely RK.

ACS Nano. 2015 Jan 27;9(1):809-16. doi: 10.1021/nn5063497. Epub 2015 Jan 8.

31.

High-resolution single-molecule fluorescence imaging of zeolite aggregates within real-life fluid catalytic cracking particles.

Ristanović Z, Kerssens MM, Kubarev AV, Hendriks FC, Dedecker P, Hofkens J, Roeffaers MB, Weckhuysen BM.

Angew Chem Int Ed Engl. 2015 Feb 2;54(6):1836-40. doi: 10.1002/anie.201410236. Epub 2014 Dec 12.

32.

PcSOFI as a smart label-based superresolution microscopy technique.

Moeyaert B, Dedecker P.

Methods Mol Biol. 2014;1148:261-76. doi: 10.1007/978-1-4939-0470-9_17.

PMID:
24718807
33.

HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme.

Borrenberghs D, Thys W, Rocha S, Demeulemeester J, Weydert C, Dedecker P, Hofkens J, Debyser Z, Hendrix J.

ACS Nano. 2014 Apr 22;8(4):3531-45. doi: 10.1021/nn406615v. Epub 2014 Apr 2.

34.

Green-to-red photoconvertible Dronpa mutant for multimodal super-resolution fluorescence microscopy.

Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, van Meervelt L, Hofkens J, Dedecker P.

ACS Nano. 2014 Feb 25;8(2):1664-73. doi: 10.1021/nn4060144. Epub 2014 Jan 21.

PMID:
24410188
35.

Excited state dynamics of the photoconvertible fluorescent protein Kaede revealed by ultrafast spectroscopy.

Fron E, Sliwa M, Adam V, Michiels J, Rocha S, Dedecker P, Hofkens J, Mizuno H.

Photochem Photobiol Sci. 2014 Jun;13(6):867-74. doi: 10.1039/c3pp50335f.

PMID:
24365976
36.

Excited state dynamics of photoswitchable fluorescent protein Padron.

Fron E, Van der Auweraer M, Hofkens J, Dedecker P.

J Phys Chem B. 2013 Dec 27;117(51):16422-7. doi: 10.1021/jp409654f. Epub 2013 Dec 13.

PMID:
24308373
37.

Fluorescent proteins: shine on, you crazy diamond.

Dedecker P, De Schryver FC, Hofkens J.

J Am Chem Soc. 2013 Feb 20;135(7):2387-402. doi: 10.1021/ja309768d. Epub 2013 Feb 5.

PMID:
23317378
38.

Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy.

Dedecker P, Duwé S, Neely RK, Zhang J.

J Biomed Opt. 2012 Dec;17(12):126008. doi: 10.1117/1.JBO.17.12.126008.

39.

Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa.

Nguyen Bich N, Moeyaert B, Van Hecke K, Dedecker P, Mizuno H, Hofkens J, Van Meervelt L.

Acta Crystallogr D Biol Crystallogr. 2012 Dec;68(Pt 12):1653-9. doi: 10.1107/S0907444912039686. Epub 2012 Nov 9.

PMID:
23151630
40.

Widely accessible method for superresolution fluorescence imaging of living systems.

Dedecker P, Mo GC, Dertinger T, Zhang J.

Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10909-14. doi: 10.1073/pnas.1204917109. Epub 2012 Jun 18.

41.

Spectroscopic characterization of Venus at the single molecule level.

David CC, Dedecker P, De Cremer G, Verstraeten N, Kint C, Michiels J, Hofkens J.

Photochem Photobiol Sci. 2012 Feb;11(2):358-63. doi: 10.1039/c1pp05292f. Epub 2012 Jan 3.

PMID:
22212707
42.

Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications.

Adam V, Moeyaert B, David CC, Mizuno H, Lelimousin M, Dedecker P, Ando R, Miyawaki A, Michiels J, Engelborghs Y, Hofkens J.

Chem Biol. 2011 Oct 28;18(10):1241-51. doi: 10.1016/j.chembiol.2011.08.007.

43.

Single-molecule spectroscopy: caught in a trap.

Dedecker P, Hofkens J.

Nat Chem. 2010 Mar;2(3):157-9. doi: 10.1038/nchem.562. No abstract available.

PMID:
21124467
44.

Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores.

Hotta J, Fron E, Dedecker P, Janssen KP, Li C, Müllen K, Harke B, Bückers J, Hell SW, Hofkens J.

J Am Chem Soc. 2010 Apr 14;132(14):5021-3. doi: 10.1021/ja100079w.

PMID:
20307062
45.

Higher resolution in localization microscopy by slower switching of a photochromic protein.

Mizuno H, Dedecker P, Ando R, Fukano T, Hofkens J, Miyawaki A.

Photochem Photobiol Sci. 2010 Feb;9(2):239-48. doi: 10.1039/b9pp00124g. Epub 2010 Jan 18.

PMID:
20126801
46.

High-resolution single-turnover mapping reveals intraparticle diffusion limitation in Ti-MCM-41-catalyzed epoxidation.

De Cremer G, Roeffaers MB, Bartholomeeusen E, Lin K, Dedecker P, Pescarmona PP, Jacobs PA, De Vos DE, Hofkens J, Sels BF.

Angew Chem Int Ed Engl. 2010;49(5):908-11. doi: 10.1002/anie.200905039. No abstract available.

PMID:
20029861
47.

Super-resolution reactivity mapping of nanostructured catalyst particles.

Roeffaers MB, De Cremer G, Libeert J, Ameloot R, Dedecker P, Bons AJ, Bückins M, Martens JA, Sels BF, De Vos DE, Hofkens J.

Angew Chem Int Ed Engl. 2009;48(49):9285-9. doi: 10.1002/anie.200904944. No abstract available.

PMID:
19890928
48.

Synthesis, spectroscopy, crystal structure, electrochemistry, and quantum chemical and molecular dynamics calculations of a 3-anilino difluoroboron dipyrromethene dye.

Qin W, Leen V, Rohand T, Dehaen W, Dedecker P, Van der Auweraer M, Robeyns K, Van Meervelt L, Beljonne D, Van Averbeke B, Clifford JN, Driesen K, Binnemans K, Boens N.

J Phys Chem A. 2009 Jan 15;113(2):439-47. doi: 10.1021/jp8077584.

PMID:
19099456
49.

How Is cis-trans Isomerization Controlled in Dronpa Mutants? A Replica Exchange Molecular Dynamics Study.

Moors SL, Michielssens S, Flors C, Dedecker P, Hofkens J, Ceulemans A.

J Chem Theory Comput. 2008 Jun;4(6):1012-20. doi: 10.1021/ct8000359.

PMID:
26621241
50.

Dark states in monomeric red fluorescent proteins studied by fluorescence correlation and single molecule spectroscopy.

Hendrix J, Flors C, Dedecker P, Hofkens J, Engelborghs Y.

Biophys J. 2008 May 15;94(10):4103-13. doi: 10.1529/biophysj.107.123596. Epub 2008 Jan 30.

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