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Genetics. 2019 Sep 3. pii: genetics.302594.2019. doi: 10.1534/genetics.119.302594. [Epub ahead of print]

TeoNAM: A Nested Association Mapping Population for Domestication and Agronomic Trait Analysis in Maize.

Author information

1
University of Wisconsin-Madison; China Agricultural University.
2
University of Wisconsin-Madison.
3
University of Wisconsin-Madison; Shanxi Normal University.
4
China Agricultural University.
5
University of Wisconsin-Madison jdoebley@wisc.edu.

Abstract

Recombinant inbred lines (RILs) are an important resource for mapping genes controlling complex traits in many species. While RIL populations have been developed for maize, a maize RIL population with multiple teosinte inbred lines as parents has been lacking. Here, we report a teosinte nested association mapping population (TeoNAM), derived from crossing five teosinte inbreds to the maize inbred line W22. The resulting 1257 BC1S4 RILs were genotyped with 51,544 SNPs, providing a high-density genetic map with a length of 1540 cM. On average, each RIL is 15% homozygous teosinte and 8% heterozygous. We performed joint linkage mapping (JLM) and genome-wide association study (GWAS) for 22 domestication and agronomic traits. A total of 255 QTLs from JLM were identified with many of these mapping near known genes or novel candidate genes. TeoNAM is a useful resource for QTL mapping for the discovery of novel allelic variation from teosinte. TeoNAM provides the first report that PROSTRATE GROWTH1, a rice domestication gene, is also a QTL associated with tillering in teosinte and maize. We detected multiple QTLs for flowering time and other traits for which the teosinte allele contributes to a more maize-like phenotype. Such QTL could be valuable in maize improvement.

KEYWORDS:

GWAS; JLM; RIL; TeoNAM; domestication; maize

PMID:
31481533
DOI:
10.1534/genetics.119.302594
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