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Items: 1 to 50 of 114


A cell-free biosynthesis platform for modular construction of protein glycosylation pathways.

Kightlinger W, Duncker KE, Ramesh A, Thames AH, Natarajan A, Stark JC, Yang A, Lin L, Mrksich M, DeLisa MP, Jewett MC.

Nat Commun. 2019 Nov 27;10(1):5404. doi: 10.1038/s41467-019-12024-9.


Broad-Spectrum Proteome Editing with an Engineered Bacterial Ubiquitin Ligase Mimic.

Ludwicki MB, Li J, Stephens EA, Roberts RW, Koide S, Hammond PT, DeLisa MP.

ACS Cent Sci. 2019 May 22;5(5):852-866. doi: 10.1021/acscentsci.9b00127. Epub 2019 May 10.


Dual Site-Specific Antibody Conjugates for Sequential and Orthogonal Cargo Release.

Thornlow DN, Cox EC, Walker JA, Sorkin M, Plesset JB, DeLisa MP, Alabi CA.

Bioconjug Chem. 2019 Jun 19;30(6):1702-1710. doi: 10.1021/acs.bioconjchem.9b00244. Epub 2019 May 24.


A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins.

Meksiriporn B, Ludwicki MB, Stephens EA, Jiang A, Lee HC, Waraho-Zhmayev D, Kummer L, Brandl F, Plückthun A, DeLisa MP.

Nat Commun. 2019 Apr 23;10(1):1830. doi: 10.1038/s41467-019-09854-y.


Improving designer glycan production in Escherichia coli through model-guided metabolic engineering.

Wayman JA, Glasscock C, Mansell TJ, DeLisa MP, Varner JD.

Metab Eng Commun. 2019 Mar 29;9:e00088. doi: 10.1016/j.mec.2019.e00088. eCollection 2019 Dec.


Antibody-Mediated Endocytosis of Polysialic Acid Enables Intracellular Delivery and Cytotoxicity of a Glycan-Directed Antibody-Drug Conjugate.

Cox EC, Thornlow DN, Jones MA, Fuller JL, Merritt JH, Paszek MJ, Alabi CA, DeLisa MP.

Cancer Res. 2019 Apr 15;79(8):1810-1821. doi: 10.1158/0008-5472.CAN-18-3119. Epub 2019 Feb 26.


Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters.

Yates LE, Natarajan A, Li M, Hale ME, Mills DC, DeLisa MP.

Metab Eng. 2019 May;53:59-68. doi: 10.1016/j.ymben.2019.02.002. Epub 2019 Feb 14.


Engineering a new generation of carbohydrate-based vaccines.

Weyant KB, Mills DC, DeLisa MP.

Curr Opin Chem Eng. 2018 Mar;19:77-85. doi: 10.1016/j.coche.2017.12.009. Epub 2018 Jan 11.


Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein.

Mahajan SP, Meksiriporn B, Waraho-Zhmayev D, Weyant KB, Kocer I, Butler DC, Messer A, Escobedo FA, DeLisa MP.

Sci Rep. 2018 Dec 4;8(1):17611. doi: 10.1038/s41598-018-35464-7.


Interplay of Carbohydrate and Carrier in Antibacterial Glycoconjugate Vaccines.

Moeller TD, Weyant KB, DeLisa MP.

Adv Biochem Eng Biotechnol. 2018 Aug 25. doi: 10.1007/10_2018_71. [Epub ahead of print]


Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

Jaroentomeechai T, Stark JC, Natarajan A, Glasscock CJ, Yates LE, Hsu KJ, Mrksich M, Jewett MC, DeLisa MP.

Nat Commun. 2018 Aug 20;9(1):3396. doi: 10.1038/s41467-018-05620-8.


Bacterial Glycoengineering as a Biosynthetic Route to Customized Glycomolecules.

Yates LE, Mills DC, DeLisa MP.

Adv Biochem Eng Biotechnol. 2018 Aug 12. doi: 10.1007/10_2018_72. [Epub ahead of print]


Design and Functional Characterization of Synthetic E3 Ubiquitin Ligases for Targeted Protein Depletion.

Baltz MR, Stephens EA, DeLisa MP.

Curr Protoc Chem Biol. 2018 Mar;10(1):72-90. doi: 10.1002/cpch.37.


Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

Jaroentomeechai T, Stark JC, Natarajan A, Glasscock CJ, Yates LE, Hsu KJ, Mrksich M, Jewett MC, DeLisa MP.

Nat Commun. 2018 Jul 12;9(1):2686. doi: 10.1038/s41467-018-05110-x. Erratum in: Nat Commun. 2018 Aug 20;9(1):3396.


Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.

Kightlinger W, Lin L, Rosztoczy M, Li W, DeLisa MP, Mrksich M, Jewett MC.

Nat Chem Biol. 2018 Jun;14(6):627-635. doi: 10.1038/s41589-018-0051-2. Epub 2018 May 7.


A flow cytometric approach to engineering Escherichia coli for improved eukaryotic protein glycosylation.

Glasscock CJ, Yates LE, Jaroentomeechai T, Wilson JD, Merritt JH, Lucks JB, DeLisa MP.

Metab Eng. 2018 May;47:488-495. doi: 10.1016/j.ymben.2018.04.014. Epub 2018 Apr 25.


Immunization with outer membrane vesicles displaying conserved surface polysaccharide antigen elicits broadly antimicrobial antibodies.

Stevenson TC, Cywes-Bentley C, Moeller TD, Weyant KB, Putnam D, Chang YF, Jones BD, Pier GB, DeLisa MP.

Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3106-E3115. doi: 10.1073/pnas.1718341115. Epub 2018 Mar 19.


A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases.

Schoborg JA, Hershewe JM, Stark JC, Kightlinger W, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa MP, Jewett MC.

Biotechnol Bioeng. 2018 Mar;115(3):739-750. doi: 10.1002/bit.26502. Epub 2017 Dec 26.


A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors.

Hamilton BS, Wilson JD, Shumakovich MA, Fisher AC, Brooks JC, Pontes A, Naran R, Heiss C, Gao C, Kardish R, Heimburg-Molinaro J, Azadi P, Cummings RD, Merritt JH, DeLisa MP.

Sci Rep. 2017 Nov 21;7(1):15907. doi: 10.1038/s41598-017-15891-8.


A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases.

Jaroentomeechai T, Zheng X, Hershewe J, Stark JC, Jewett MC, DeLisa MP.

Methods Enzymol. 2017;597:55-81. doi: 10.1016/bs.mie.2017.07.011. Epub 2017 Sep 13.


A single dose and long lasting vaccine against pandemic influenza through the controlled release of a heterospecies tandem M2 sequence embedded within detoxified bacterial outer membrane vesicles.

Watkins HC, Pagan CL, Childs HR, Posada S, Chau A, Rios J, Guarino C, DeLisa MP, Whittaker GR, Putnam D.

Vaccine. 2017 Sep 25;35(40):5373-5380. doi: 10.1016/j.vaccine.2017.08.013. Epub 2017 Aug 31.


A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo.

Mizrachi D, Robinson MP, Ren G, Ke N, Berkmen M, DeLisa MP.

Nat Chem Biol. 2017 Sep;13(9):1022-1028. doi: 10.1038/nchembio.2409. Epub 2017 Jun 19.


Designer outer membrane vesicles as immunomodulatory systems - Reprogramming bacteria for vaccine delivery.

Gnopo YMD, Watkins HC, Stevenson TC, DeLisa MP, Putnam D.

Adv Drug Deliv Rev. 2017 May 15;114:132-142. doi: 10.1016/j.addr.2017.05.003. Epub 2017 May 10. Review.


Safe Recombinant Outer Membrane Vesicles that Display M2e Elicit Heterologous Influenza Protection.

Watkins HC, Rappazzo CG, Higgins JS, Sun X, Brock N, Chau A, Misra A, Cannizzo JPB, King MR, Maines TR, Leifer CA, Whittaker GR, DeLisa MP, Putnam D.

Mol Ther. 2017 Apr 5;25(4):989-1002. doi: 10.1016/j.ymthe.2017.01.010. Epub 2017 Feb 16.


An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in Escherichia coli.

Natarajan A, Haitjema CH, Lee R, Boock JT, DeLisa MP.

ACS Synth Biol. 2017 May 19;6(5):875-883. doi: 10.1021/acssynbio.6b00366. Epub 2017 Feb 16.


A Molecularly Complete Planar Bacterial Outer Membrane Platform.

Hsia CY, Chen L, Singh RR, DeLisa MP, Daniel S.

Sci Rep. 2016 Sep 7;6:32715. doi: 10.1038/srep32715.


Immunization with Outer Membrane Vesicles Displaying Designer Glycotopes Yields Class-Switched, Glycan-Specific Antibodies.

Valentine JL, Chen L, Perregaux EC, Weyant KB, Rosenthal JA, Heiss C, Azadi P, Fisher AC, Putnam D, Moe GR, Merritt JH, DeLisa MP.

Cell Chem Biol. 2016 Jun 23;23(6):655-65. doi: 10.1016/j.chembiol.2016.05.014.


Outer membrane vesicles displaying engineered glycotopes elicit protective antibodies.

Chen L, Valentine JL, Huang CJ, Endicott CE, Moeller TD, Rasmussen JA, Fletcher JR, Boll JM, Rosenthal JA, Dobruchowska J, Wang Z, Heiss C, Azadi P, Putnam D, Trent MS, Jones BD, DeLisa MP.

Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):E3609-18. doi: 10.1073/pnas.1518311113. Epub 2016 Jun 6.


Engineered Protein Machines: Emergent Tools for Synthetic Biology.

Glasscock CJ, Lucks JB, DeLisa MP.

Cell Chem Biol. 2016 Jan 21;23(1):45-56. doi: 10.1016/j.chembiol.2015.12.004. Review.


Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice.

Rappazzo CG, Watkins HC, Guarino CM, Chau A, Lopez JL, DeLisa MP, Leifer CA, Whittaker GR, Putnam D.

Vaccine. 2016 Mar 4;34(10):1252-8. doi: 10.1016/j.vaccine.2016.01.028. Epub 2016 Jan 29.


Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences.

Ollis AA, Chai Y, Natarajan A, Perregaux E, Jaroentomeechai T, Guarino C, Smith J, Zhang S, DeLisa MP.

Sci Rep. 2015 Oct 20;5:15237. doi: 10.1038/srep15237.


Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria.

Robinson MP, Ke N, Lobstein J, Peterson C, Szkodny A, Mansell TJ, Tuckey C, Riggs PD, Colussi PA, Noren CJ, Taron CH, DeLisa MP, Berkmen M.

Nat Commun. 2015 Aug 27;6:8072. doi: 10.1038/ncomms9072.


GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes.

Ollis AA, Chai Y, DeLisa MP.

Methods Mol Biol. 2015;1321:37-47. doi: 10.1007/978-1-4939-2760-9_3.


Characterizing Metal-Dependent Nucleases of CRISPR-Cas Prokaryotic Adaptive Immunity Systems.

Nam KH, DeLisa MP, Ke A.

Methods Mol Biol. 2015;1311:265-76. doi: 10.1007/978-1-4939-2687-9_17.


Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy.

Mizrachi D, Chen Y, Liu J, Peng HM, Ke A, Pollack L, Turner RJ, Auchus RJ, DeLisa MP.

Nat Commun. 2015 Apr 8;6:6826. doi: 10.1038/ncomms7826.


Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells.

Boock JT, King BC, Taw MN, Conrado RJ, Siu KH, Stark JC, Walker LP, Gibson DM, DeLisa MP.

J Mol Biol. 2015 Mar 27;427(6 Pt B):1451-1463. doi: 10.1016/j.jmb.2015.01.003. Epub 2015 Jan 12.


An engineered genetic selection for ternary protein complexes inspired by a natural three-component hitchhiker mechanism.

Lee HC, Portnoff AD, Rocco MA, DeLisa MP.

Sci Rep. 2014 Dec 22;4:7570. doi: 10.1038/srep07570.


Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them.

Boock JT, Waraho-Zhmayev D, Mizrachi D, DeLisa MP.

Methods Mol Biol. 2015;1258:79-97. doi: 10.1007/978-1-4939-2205-5_5. Review.


Mechanistic insight into the TH1-biased immune response to recombinant subunit vaccines delivered by probiotic bacteria-derived outer membrane vesicles.

Rosenthal JA, Huang CJ, Doody AM, Leung T, Mineta K, Feng DD, Wayne EC, Nishimura N, Leifer C, DeLisa MP, Mendez S, Putnam D.

PLoS One. 2014 Nov 26;9(11):e112802. doi: 10.1371/journal.pone.0112802. eCollection 2014.


Glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions.

Çelik E, Ollis AA, Lasanajak Y, Fisher AC, Gür G, Smith DF, DeLisa MP.

Biotechnol J. 2015 Jan;10(1):199-209. doi: 10.1002/biot.201400354. Epub 2014 Oct 31.


Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection.

Waraho-Zhmayev D, Meksiriporn B, Portnoff AD, DeLisa MP.

Protein Eng Des Sel. 2014 Oct;27(10):351-8. doi: 10.1093/protein/gzu038. Epub 2014 Sep 14.


Engineered oligosaccharyltransferases with greatly relaxed acceptor-site specificity.

Ollis AA, Zhang S, Fisher AC, DeLisa MP.

Nat Chem Biol. 2014 Oct;10(10):816-22. doi: 10.1038/nchembio.1609. Epub 2014 Aug 17.


Pathogen-like particles: biomimetic vaccine carriers engineered at the nanoscale.

Rosenthal JA, Chen L, Baker JL, Putnam D, DeLisa MP.

Curr Opin Biotechnol. 2014 Aug;28:51-8. doi: 10.1016/j.copbio.2013.11.005. Epub 2013 Dec 7. Review.


Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions.

Park M, Sun Q, Liu F, DeLisa MP, Chen W.

PLoS One. 2014 May 12;9(5):e97103. doi: 10.1371/journal.pone.0097103. eCollection 2014.


Microbial biosynthesis of designer outer membrane vesicles.

Baker JL, Chen L, Rosenthal JA, Putnam D, DeLisa MP.

Curr Opin Biotechnol. 2014 Oct;29:76-84. doi: 10.1016/j.copbio.2014.02.018. Epub 2014 Mar 22. Review.


Ubiquibodies, synthetic E3 ubiquitin ligases endowed with unnatural substrate specificity for targeted protein silencing.

Portnoff AD, Stephens EA, Varner JD, DeLisa MP.

J Biol Chem. 2014 Mar 14;289(11):7844-55. doi: 10.1074/jbc.M113.544825. Epub 2014 Jan 28.


Split-Cre recombinase effectively monitors protein-protein interactions in living bacteria.

O'Brien SP, DeLisa MP.

Biotechnol J. 2014 Mar;9(3):355-61. doi: 10.1002/biot.201300462. Epub 2014 Jan 29.


Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis.

Sun Q, Madan B, Tsai SL, DeLisa MP, Chen W.

Chem Commun (Camb). 2014 Feb 11;50(12):1423-5. doi: 10.1039/c3cc47215a.


Universal genetic assay for engineering extracellular protein expression.

Haitjema CH, Boock JT, Natarajan A, Dominguez MA, Gardner JG, Keating DH, Withers ST, DeLisa MP.

ACS Synth Biol. 2014 Feb 21;3(2):74-82. doi: 10.1021/sb400142b. Epub 2013 Nov 22.


Directed evolution of Mycobacterium tuberculosis β-lactamase reveals gatekeeper residue that regulates antibiotic resistance and catalytic efficiency.

Feiler C, Fisher AC, Boock JT, Marrichi MJ, Wright L, Schmidpeter PA, Blankenfeldt W, Pavelka M, DeLisa MP.

PLoS One. 2013 Sep 4;8(9):e73123. doi: 10.1371/journal.pone.0073123. eCollection 2013.

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