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Nucleic Acids Res. 2018 Apr 6;46(6):2701-2721. doi: 10.1093/nar/gky167.

Capturing the 'ome': the expanding molecular toolbox for RNA and DNA library construction.

Author information

1
Center for Medical Biotechnology, VIB, Zwijnaarde 9052, Belgium.
2
Department of Biochemistry and Microbiology, Ghent University, Ghent 9000, Belgium.

Abstract

All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences. In the past decade especially, driven by the progress in the field of massively parallel sequencing, numerous studies have comprehensively assessed the impact of particular manipulations on library complexity and quality, and characterized the activities and specificities of several key enzymes used in library construction. Fortunately, careful protocol design and reagent choice can substantially mitigate many of these biases, and enable reliable representation of sequences in libraries. This review aims to guide the reader through the vast expanse of literature on the subject to promote informed library generation, independent of the application.

PMID:
29514322
PMCID:
PMC5888575
DOI:
10.1093/nar/gky167
[Indexed for MEDLINE]
Free PMC Article

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