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Items: 1 to 50 of 83

1.

Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.

Gand M, Mattheus W, Saltykova A, Roosens N, Dierick K, Marchal K, De Keersmaecker SCJ, Bertrand S.

Appl Microbiol Biotechnol. 2019 Jun;103(12):4987-4996. doi: 10.1007/s00253-019-09854-4. Epub 2019 May 6.

2.

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

Ventola E, Bogaerts B, De Keersmaecker SCJ, Vanneste K, Roosens NHC, Mattheus W, Ceyssens PJ.

Microbiologyopen. 2019 Mar 28:e00807. doi: 10.1002/mbo3.807. [Epub ahead of print]

3.

Validation of a Bioinformatics Workflow for Routine Analysis of Whole-Genome Sequencing Data and Related Challenges for Pathogen Typing in a European National Reference Center: Neisseria meningitidis as a Proof-of-Concept.

Bogaerts B, Winand R, Fu Q, Van Braekel J, Ceyssens PJ, Mattheus W, Bertrand S, De Keersmaecker SCJ, Roosens NHC, Vanneste K.

Front Microbiol. 2019 Mar 6;10:362. doi: 10.3389/fmicb.2019.00362. eCollection 2019.

4.

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

Fraiture MA, Saltykova A, Hoffman S, Winand R, Deforce D, Vanneste K, De Keersmaecker SCJ, Roosens NHC.

Sci Rep. 2018 May 21;8(1):7903. doi: 10.1038/s41598-018-26259-x.

5.

High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei.

Girault G, Wattiau P, Saqib M, Martin B, Vorimore F, Singha H, Engelsma M, Roest HJ, Spicic S, Grunow R, Vicari N, De Keersmaecker SCJ, Roosens NHC, Fabbi M, Tripathi BN, Zientara S, Madani N, Laroucau K.

Infect Genet Evol. 2018 Sep;63:1-4. doi: 10.1016/j.meegid.2018.05.004. Epub 2018 May 8.

PMID:
29751195
6.

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system.

Barbau-Piednoir E, Denayer S, Botteldoorn N, Dierick K, De Keersmaecker SCJ, Roosens NH.

Appl Microbiol Biotechnol. 2018 Apr;102(7):3267-3285. doi: 10.1007/s00253-018-8820-0. Epub 2018 Feb 20.

7.

A novel genotoxin-specific qPCR array based on the metabolically competent human HepaRG cell line as a rapid and reliable tool for improved in vitro hazard assessment.

Ates G, Mertens B, Heymans A, Verschaeve L, Milushev D, Vanparys P, Roosens NHC, De Keersmaecker SCJ, Rogiers V, Doktorova TY.

Arch Toxicol. 2018 Apr;92(4):1593-1608. doi: 10.1007/s00204-018-2172-5. Epub 2018 Feb 6.

PMID:
29411056
8.

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

Saltykova A, Wuyts V, Mattheus W, Bertrand S, Roosens NHC, Marchal K, De Keersmaecker SCJ.

PLoS One. 2018 Feb 6;13(2):e0192504. doi: 10.1371/journal.pone.0192504. eCollection 2018.

9.

The genetic structure of the Belgian population.

Van den Eynden J, Descamps T, Delporte E, Roosens NHC, De Keersmaecker SCJ, De Wit V, Vermeesch JR, Goetghebeur E, Tafforeau J, Demarest S, Van den Bulcke M, Van Oyen H.

Hum Genomics. 2018 Feb 2;12(1):6. doi: 10.1186/s40246-018-0136-8.

10.

Detection of Plasmid-Mediated Colistin Resistance, mcr-1 and mcr-2 Genes, in Salmonella spp. Isolated from Food at Retail in Belgium from 2012 to 2015.

Garcia-Graells C, De Keersmaecker SCJ, Vanneste K, Pochet B, Vermeersch K, Roosens N, Dierick K, Botteldoorn N.

Foodborne Pathog Dis. 2018 Feb;15(2):114-117. doi: 10.1089/fpd.2017.2329. Epub 2017 Nov 28.

PMID:
29182477
11.

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

Wuyts V, Mattheus W, Roosens NHC, Marchal K, Bertrand S, De Keersmaecker SCJ.

Methods Mol Biol. 2017;1616:39-69. doi: 10.1007/978-1-4939-7037-7_3.

PMID:
28600761
12.

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

Libert X, Packeu A, Bureau F, Roosens NH, De Keersmaecker SC.

BMC Microbiol. 2017 Apr 4;17(1):84. doi: 10.1186/s12866-017-0996-4.

13.

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

Libert X, Packeu A, Bureau F, Roosens NH, De Keersmaecker SC.

PLoS One. 2017 Mar 9;12(3):e0173390. doi: 10.1371/journal.pone.0173390. eCollection 2017.

14.

FabR regulates Salmonella biofilm formation via its direct target FabB.

Hermans K, Roberfroid S, Thijs IM, Kint G, De Coster D, Marchal K, Vanderleyden J, De Keersmaecker SC, Steenackers HP.

BMC Genomics. 2016 Mar 22;17:253. doi: 10.1186/s12864-016-2387-x.

15.

Optimized MOL-PCR for Characterization of Microbial Pathogens.

Wuyts V, Roosens NH, Bertrand S, Marchal K, De Keersmaecker SC.

Curr Protoc Cytom. 2016 Jan 6;75:13.15.1-13.15.15. doi: 10.1002/0471142956.cy1315s75.

PMID:
26742655
16.

Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.

Barbau-Piednoir E, De Keersmaecker SC, Delvoye M, Gau C, Philipp P, Roosens NH.

BMC Biotechnol. 2015 Nov 11;15:103. doi: 10.1186/s12896-015-0216-y.

17.

Whole Genome Sequence Analysis of Salmonella Enteritidis PT4 Outbreaks from a National Reference Laboratory's Viewpoint.

Wuyts V, Denayer S, Roosens NH, Mattheus W, Bertrand S, Marchal K, Dierick K, De Keersmaecker SC.

PLoS Curr. 2015 Sep 11;7. pii: ecurrents.outbreaks.aa5372d90826e6cb0136ff66bb7a62fc. doi: 10.1371/currents.outbreaks.aa5372d90826e6cb0136ff66bb7a62fc.

18.

Statistical framework for detection of genetically modified organisms based on Next Generation Sequencing.

Willems S, Fraiture MA, Deforce D, De Keersmaecker SC, De Loose M, Ruttink T, Herman P, Van Nieuwerburgh F, Roosens N.

Food Chem. 2016 Feb 1;192:788-98. doi: 10.1016/j.foodchem.2015.07.074. Epub 2015 Jul 21.

19.

Erratum to: Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Libert X, Chasseur C, Bladt S, Packeu A, Bureau F, Roosens NH, De Keersmaecker SC.

Appl Microbiol Biotechnol. 2015 Oct;99(19):8319-20. doi: 10.1007/s00253-015-6932-3. No abstract available.

20.

A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium.

Wuyts V, Mattheus W, Roosens NH, Marchal K, Bertrand S, De Keersmaecker SC.

Appl Microbiol Biotechnol. 2015 Oct;99(19):8137-49. doi: 10.1007/s00253-015-6831-7. Epub 2015 Jul 25.

21.

Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Libert X, Chasseur C, Bladt S, Packeu A, Bureau F, Roosens NH, De Keersmaecker SC.

Appl Microbiol Biotechnol. 2015 Sep;99(17):7267-82. doi: 10.1007/s00253-015-6785-9. Epub 2015 Jul 17. Erratum in: Appl Microbiol Biotechnol. 2015 Oct;99(19):8319-20.

22.

Experimental approaches to identify small RNAs and their diverse roles in bacteria--what we have learnt in one decade of MicA research.

Van Puyvelde S, Vanderleyden J, De Keersmaecker SC.

Microbiologyopen. 2015 Oct;4(5):699-711. doi: 10.1002/mbo3.263. Epub 2015 May 13. Review.

23.

Genome Sequence of EU-Unauthorized Genetically Modified Bacillus subtilis Strain 2014-3557 Overproducing Riboflavin, Isolated from a Vitamin B2 80% Feed Additive.

Barbau-Piednoir E, De Keersmaecker SC, Wuyts V, Gau C, Pirovano W, Costessi A, Philipp P, Roosens NH.

Genome Announc. 2015 Apr 9;3(2). pii: e00214-15. doi: 10.1128/genomeA.00214-15.

24.

Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.

Wuyts V, Roosens NH, Bertrand S, Marchal K, De Keersmaecker SC.

Biomed Res Int. 2015;2015:790170. doi: 10.1155/2015/790170. Epub 2015 Feb 3.

25.

Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies.

El Bali L, Diman A, Bernard A, Roosens NH, De Keersmaecker SC.

J Biomol Tech. 2014 Dec;25(4):96-110. doi: 10.7171/jbt.14-2504-002.

26.

A GFP promoter fusion library for the study of Salmonella biofilm formation and the mode of action of biofilm inhibitors.

Robijns SC, Roberfroid S, Van Puyvelde S, De Pauw B, Uceda Santamaría E, De Weerdt A, De Coster D, Hermans K, De Keersmaecker SC, Vanderleyden J, Steenackers HP.

Biofouling. 2014;30(5):605-25. doi: 10.1080/08927014.2014.907401. Epub 2014 Apr 15.

PMID:
24735176
27.

Genome Sequence of the Salmonella enterica subsp. enterica Serovar Namur Strain 05-2929, Lacking the Salmonella Atypical Fimbrial Operon.

Barbau-Piednoir E, Bertrand S, Roosens NH, De Keersmaecker SC.

Genome Announc. 2014 Apr 10;2(2). pii: e00299-14. doi: 10.1128/genomeA.00299-14.

28.

MLVA as a tool for public health surveillance of human Salmonella Typhimurium: prospective study in Belgium and evaluation of MLVA loci stability.

Wuyts V, Mattheus W, De Laminne de Bex G, Wildemauwe C, Roosens NH, Marchal K, De Keersmaecker SC, Bertrand S.

PLoS One. 2013 Dec 31;8(12):e84055. doi: 10.1371/journal.pone.0084055. eCollection 2013.

29.

Lipoteichoic acid is an important microbe-associated molecular pattern of Lactobacillus rhamnosus GG.

Claes IJ, Segers ME, Verhoeven TL, Dusselier M, Sels BF, De Keersmaecker SC, Vanderleyden J, Lebeer S.

Microb Cell Fact. 2012 Dec 15;11:161. doi: 10.1186/1475-2859-11-161.

30.

How to deal with the upcoming challenges in GMO detection in food and feed.

Broeders SR, De Keersmaecker SC, Roosens NH.

J Biomed Biotechnol. 2012;2012:402418. doi: 10.1155/2012/402418. Epub 2012 Oct 21. Review.

31.

Towards understanding gene expression in multispecies biofilms containing Salmonella typhimurium.

Roberfroid S, Hermans K, Robijns SC, Steenackers HP, Vanderleyden J, De Keersmaecker SC.

Commun Agric Appl Biol Sci. 2012;77(1):33-7. No abstract available.

PMID:
22558752
32.

A combined approach to study the protein glycosylation potential of Lactobacillus rhamnosus GG (LGG).

Tytgat HL, Sánchez-Rodríguez A, Schoofs G, Verhoeven TL, De Keersmaecker SC, Marchal K, Vanderleyden J, Lebeer S.

Commun Agric Appl Biol Sci. 2012;77(1):15-9. No abstract available.

PMID:
22558749
33.

Identification and characterization of 4-[4-(3-phenyl-2-propen-1-yl)-1-piperazinyl]-5H-pyrimido[5,4-b]indole derivatives as Salmonella biofilm inhibitors.

Robijns SC, De Pauw B, Loosen B, Marchand A, Chaltin P, De Keersmaecker SC, Vanderleyden J, Steenackers HP.

FEMS Immunol Med Microbiol. 2012 Jul;65(2):390-4. doi: 10.1111/j.1574-695X.2012.00973.x. Epub 2012 May 8.

34.

Genetic and biochemical characterization of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG.

Claes IJ, Schoofs G, Regulski K, Courtin P, Chapot-Chartier MP, Rolain T, Hols P, von Ossowski I, Reunanen J, de Vos WM, Palva A, Vanderleyden J, De Keersmaecker SC, Lebeer S.

PLoS One. 2012;7(2):e31588. doi: 10.1371/journal.pone.0031588. Epub 2012 Feb 16.

35.

The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG.

Lebeer S, Claes IJ, Balog CI, Schoofs G, Verhoeven TL, Nys K, von Ossowski I, de Vos WM, Tytgat HL, Agostinis P, Palva A, Van Damme EJ, Deelder AM, De Keersmaecker SC, Wuhrer M, Vanderleyden J.

Microb Cell Fact. 2012 Feb 1;11:15. doi: 10.1186/1475-2859-11-15.

36.

Adaptation factors of the probiotic Lactobacillus rhamnosus GG.

Lebeer S, Vanderleyden J, De Keersmaecker SC.

Benef Microbes. 2010 Nov;1(4):335-42. doi: 10.3920/BM2010.0032. Review.

PMID:
21831772
37.

Lessons from probiotic-host interaction studies in murine models of experimental colitis.

Claes IJ, De Keersmaecker SC, Vanderleyden J, Lebeer S.

Mol Nutr Food Res. 2011 Oct;55(10):1441-53. doi: 10.1002/mnfr.201100139. Epub 2011 Jul 28. Review.

PMID:
21796777
38.

Functional characterization of a mucus-specific LPXTG surface adhesin from probiotic Lactobacillus rhamnosus GG.

von Ossowski I, Satokari R, Reunanen J, Lebeer S, De Keersmaecker SC, Vanderleyden J, de Vos WM, Palva A.

Appl Environ Microbiol. 2011 Jul;77(13):4465-72. doi: 10.1128/AEM.02497-10. Epub 2011 May 20.

39.

Structure-activity relationship of 2-hydroxy-2-aryl-2,3-dihydro-imidazo[1,2-a]pyrimidinium salts and 2N-substituted 4(5)-aryl-2-amino-1H-imidazoles as inhibitors of biofilm formation by Salmonella Typhimurium and Pseudomonas aeruginosa.

Steenackers HP, Ermolat'ev DS, Savaliya B, Weerdt AD, Coster DD, Shah A, Van der Eycken EV, De Vos DE, Vanderleyden J, De Keersmaecker SC.

Bioorg Med Chem. 2011 Jun 1;19(11):3462-73. doi: 10.1016/j.bmc.2011.04.026. Epub 2011 Apr 19.

PMID:
21550812
40.

High-throughput screening in search of broad applicable, small molecule inhibitors of salmonella biofilm formation.

Robijns SC, Steenackers HP, Janssens JC, Marchand A, Chaltin P, Vanderleyden J, De Keersmaecker SC.

Commun Agric Appl Biol Sci. 2011;76(1):125-8. No abstract available.

PMID:
21539213
41.

Exopolysaccharides of Lactobacillus rhamnosus GG form a protective shield against innate immune factors in the intestine.

Lebeer S, Claes IJ, Verhoeven TL, Vanderleyden J, De Keersmaecker SC.

Microb Biotechnol. 2011 May;4(3):368-74. doi: 10.1111/j.1751-7915.2010.00199.x. Epub 2010 Aug 17.

42.

Gene expression analysis of monospecies Salmonella typhimurium biofilms using differential fluorescence induction.

Hermans K, Nguyen TL, Roberfroid S, Schoofs G, Verhoeven T, De Coster D, Vanderleyden J, De Keersmaecker SC.

J Microbiol Methods. 2011 Mar;84(3):467-78. doi: 10.1016/j.mimet.2011.01.012. Epub 2011 Jan 21.

PMID:
21256891
43.

A community effort towards a knowledge-base and mathematical model of the human pathogen Salmonella Typhimurium LT2.

Thiele I, Hyduke DR, Steeb B, Fankam G, Allen DK, Bazzani S, Charusanti P, Chen FC, Fleming RM, Hsiung CA, De Keersmaecker SC, Liao YC, Marchal K, Mo ML, Özdemir E, Raghunathan A, Reed JL, Shin SI, Sigurbjörnsdóttir S, Steinmann J, Sudarsan S, Swainston N, Thijs IM, Zengler K, Palsson BO, Adkins JN, Bumann D.

BMC Syst Biol. 2011 Jan 18;5:8. doi: 10.1186/1752-0509-5-8.

44.

FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts.

Lebeer S, Verhoeven TL, Claes IJ, De Hertogh G, Vermeire S, Buyse J, Van Immerseel F, Vanderleyden J, De Keersmaecker SC.

Lett Appl Microbiol. 2011 Mar;52(3):220-6. doi: 10.1111/j.1472-765X.2010.02994.x.

45.

Structure-activity relationship of 4(5)-aryl-2-amino-1H-imidazoles, N1-substituted 2-aminoimidazoles and imidazo[1,2-a]pyrimidinium salts as inhibitors of biofilm formation by Salmonella typhimurium and Pseudomonas aeruginosa.

Steenackers HP, Ermolat'ev DS, Savaliya B, De Weerdt A, De Coster D, Shah A, Van der Eycken EV, De Vos DE, Vanderleyden J, De Keersmaecker SC.

J Med Chem. 2011 Jan 27;54(2):472-84. doi: 10.1021/jm1011148. Epub 2010 Dec 20.

PMID:
21174477
46.

The small regulatory RNA molecule MicA is involved in Salmonella enterica serovar Typhimurium biofilm formation.

Kint G, De Coster D, Marchal K, Vanderleyden J, De Keersmaecker SC.

BMC Microbiol. 2010 Nov 2;10:276. doi: 10.1186/1471-2180-10-276.

47.

Concise and diversity-oriented route toward polysubstituted 2-aminoimidazole alkaloids and their analogues.

Ermolat'ev DS, Bariwal JB, Steenackers HP, De Keersmaecker SC, Van der Eycken EV.

Angew Chem Int Ed Engl. 2010 Dec 3;49(49):9465-8. doi: 10.1002/anie.201004256. No abstract available.

PMID:
20963737
48.

Impact of lipoteichoic acid modification on the performance of the probiotic Lactobacillus rhamnosus GG in experimental colitis.

Claes IJ, Lebeer S, Shen C, Verhoeven TL, Dilissen E, De Hertogh G, Bullens DM, Ceuppens JL, Van Assche G, Vermeire S, Rutgeerts P, Vanderleyden J, De Keersmaecker SC.

Clin Exp Immunol. 2010 Nov;162(2):306-14. doi: 10.1111/j.1365-2249.2010.04228.x. Epub 2010 Aug 20.

49.

The AI-2-dependent regulator LsrR has a limited regulon in Salmonella Typhimurium.

Thijs IM, Zhao H, De Weerdt A, Engelen K, De Coster D, Schoofs G, McClelland M, Vanderleyden J, Marchal K, De Keersmaecker SC.

Cell Res. 2010 Aug;20(8):966-9. doi: 10.1038/cr.2010.104. Epub 2010 Jul 13. No abstract available.

PMID:
20628367
50.

Structure-activity relationship of brominated 3-alkyl-5-methylene-2(5H)-furanones and alkylmaleic anhydrides as inhibitors of Salmonella biofilm formation and quorum sensing regulated bioluminescence in Vibrio harveyi.

Steenackers HP, Levin J, Janssens JC, De Weerdt A, Balzarini J, Vanderleyden J, De Vos DE, De Keersmaecker SC.

Bioorg Med Chem. 2010 Jul 15;18(14):5224-33. doi: 10.1016/j.bmc.2010.05.055. Epub 2010 May 25.

PMID:
20580562

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