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Structural basis for transcription activation by Crl through tethering of σS and RNA polymerase.

Cartagena AJ, Banta AB, Sathyan N, Ross W, Gourse RL, Campbell EA, Darst SA.

Proc Natl Acad Sci U S A. 2019 Sep 4. pii: 201910827. doi: 10.1073/pnas.1910827116. [Epub ahead of print]


Mechanisms of Transcriptional Pausing in Bacteria.

Kang JY, Mishanina TV, Landick R, Darst SA.

J Mol Biol. 2019 Jul 13. pii: S0022-2836(19)30446-2. doi: 10.1016/j.jmb.2019.07.017. [Epub ahead of print] Review.


Discovery and structure of the antimicrobial lasso peptide citrocin.

Cheung-Lee WL, Parry ME, Jaramillo Cartagena A, Darst SA, Link AJ.

J Biol Chem. 2019 Apr 26;294(17):6822-6830. doi: 10.1074/jbc.RA118.006494. Epub 2019 Mar 7.


Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.

Boyaci H, Chen J, Jansen R, Darst SA, Campbell EA.

Nature. 2019 Jan;565(7739):382-385. doi: 10.1038/s41586-018-0840-5. Epub 2019 Jan 9.


Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.

Braffman NR, Piscotta FJ, Hauver J, Campbell EA, Link AJ, Darst SA.

Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1273-1278. doi: 10.1073/pnas.1817352116. Epub 2019 Jan 9.


Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism.

Peek J, Lilic M, Montiel D, Milshteyn A, Woodworth I, Biggins JB, Ternei MA, Calle PY, Danziger M, Warrier T, Saito K, Braffman N, Fay A, Glickman MS, Darst SA, Campbell EA, Brady SF.

Nat Commun. 2018 Oct 8;9(1):4147. doi: 10.1038/s41467-018-06587-2.


Structural Basis for Transcript Elongation Control by NusG Family Universal Regulators.

Kang JY, Mooney RA, Nedialkov Y, Saba J, Mishanina TV, Artsimovitch I, Landick R, Darst SA.

Cell. 2018 Jun 14;173(7):1650-1662.e14. doi: 10.1016/j.cell.2018.05.017. Epub 2018 Jun 7.


RNA Polymerase Accommodates a Pause RNA Hairpin by Global Conformational Rearrangements that Prolong Pausing.

Kang JY, Mishanina TV, Bellecourt MJ, Mooney RA, Darst SA, Landick R.

Mol Cell. 2018 Mar 1;69(5):802-815.e5. doi: 10.1016/j.molcel.2018.01.018.


Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts.

Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA.

Elife. 2018 Feb 26;7. pii: e34823. doi: 10.7554/eLife.34823.


6S RNA Mimics B-Form DNA to Regulate Escherichia coli RNA Polymerase.

Chen J, Wassarman KM, Feng S, Leon K, Feklistov A, Winkelman JT, Li Z, Walz T, Campbell EA, Darst SA.

Mol Cell. 2017 Oct 19;68(2):388-397.e6. doi: 10.1016/j.molcel.2017.09.006. Epub 2017 Oct 5.


Structural insights into the mycobacteria transcription initiation complex from analysis of X-ray crystal structures.

Hubin EA, Lilic M, Darst SA, Campbell EA.

Nat Commun. 2017 Jul 13;8:16072. doi: 10.1038/ncomms16072.


RNA polymerase motions during promoter melting.

Feklistov A, Bae B, Hauver J, Lass-Napiorkowska A, Kalesse M, Glaus F, Altmann KH, Heyduk T, Landick R, Darst SA.

Science. 2017 May 26;356(6340):863-866. doi: 10.1126/science.aam7858.


Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex.

Kang JY, Olinares PD, Chen J, Campbell EA, Mustaev A, Chait BT, Gottesman ME, Darst SA.

Elife. 2017 Mar 20;6. pii: e25478. doi: 10.7554/eLife.25478.


Crystal structure of Aquifex aeolicus σN bound to promoter DNA and the structure of σN-holoenzyme.

Campbell EA, Kamath S, Rajashankar KR, Wu M, Darst SA.

Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):E1805-E1814. doi: 10.1073/pnas.1619464114. Epub 2017 Feb 21.


Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA.

Hubin EA, Fay A, Xu C, Bean JM, Saecker RM, Glickman MS, Darst SA, Campbell EA.

Elife. 2017 Jan 9;6. pii: e22520. doi: 10.7554/eLife.22520.


Single-Molecule Real-Time 3D Imaging of the Transcription Cycle by Modulation Interferometry.

Wang G, Hauver J, Thomas Z, Darst SA, Pertsinidis A.

Cell. 2016 Dec 15;167(7):1839-1852.e21. doi: 10.1016/j.cell.2016.11.032.


Effects of Increasing the Affinity of CarD for RNA Polymerase on Mycobacterium tuberculosis Growth, rRNA Transcription, and Virulence.

Garner AL, Rammohan J, Huynh JP, Onder LM, Chen J, Bae B, Jensen D, Weiss LA, Manzano AR, Darst SA, Campbell EA, Nickels BE, Galburt EA, Stallings CL.

J Bacteriol. 2017 Jan 30;199(4). pii: e00698-16. doi: 10.1128/JB.00698-16. Print 2017 Feb 15.


CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.

Bae B, Chen J, Davis E, Leon K, Darst SA, Campbell EA.

Elife. 2015 Sep 8;4. doi: 10.7554/eLife.08505.


Structure of a bacterial RNA polymerase holoenzyme open promoter complex.

Bae B, Feklistov A, Lass-Napiorkowska A, Landick R, Darst SA.

Elife. 2015 Sep 8;4. doi: 10.7554/eLife.08504.


CBR antimicrobials inhibit RNA polymerase via at least two bridge-helix cap-mediated effects on nucleotide addition.

Bae B, Nayak D, Ray A, Mustaev A, Landick R, Darst SA.

Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):E4178-87. doi: 10.1073/pnas.1502368112. Epub 2015 Jul 20.


Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA.

Hubin EA, Tabib-Salazar A, Humphrey LJ, Flack JE, Olinares PD, Darst SA, Campbell EA, Paget MS.

Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7171-6. doi: 10.1073/pnas.1504942112. Epub 2015 May 26.


TFIIB is only ∼9 Å away from the 5'-end of a trimeric RNA primer in a functional RNA polymerase II preinitiation complex.

Bick MJ, Malik S, Mustaev A, Darst SA.

PLoS One. 2015 Mar 16;10(3):e0119007. doi: 10.1371/journal.pone.0119007. eCollection 2015.


Mycobacterial RNA polymerase forms unstable open promoter complexes that are stabilized by CarD.

Davis E, Chen J, Leon K, Darst SA, Campbell EA.

Nucleic Acids Res. 2015 Jan;43(1):433-45. doi: 10.1093/nar/gku1231. Epub 2014 Dec 15.


RNA polymerase pausing and nascent-RNA structure formation are linked through clamp-domain movement.

Hein PP, Kolb KE, Windgassen T, Bellecourt MJ, Darst SA, Mooney RA, Landick R.

Nat Struct Mol Biol. 2014 Sep;21(9):794-802. doi: 10.1038/nsmb.2867. Epub 2014 Aug 10.


Bacterial sigma factors: a historical, structural, and genomic perspective.

Feklístov A, Sharon BD, Darst SA, Gross CA.

Annu Rev Microbiol. 2014;68:357-76. doi: 10.1146/annurev-micro-092412-155737. Epub 2014 Jun 18. Review.


Structure of the branched intermediate in protein splicing.

Liu Z, Frutos S, Bick MJ, Vila-Perelló M, Debelouchina GT, Darst SA, Muir TW.

Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8422-7. doi: 10.1073/pnas.1402942111. Epub 2014 Apr 28.


Promoter melting by an alternative σ, one base at a time.

Darst SA, Feklistov A, Gross CA.

Nat Struct Mol Biol. 2014 Apr;21(4):350-1. doi: 10.1038/nsmb.2798. No abstract available.


Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of σ70 domain 1.1.

Bae B, Davis E, Brown D, Campbell EA, Wigneshweraraj S, Darst SA.

Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19772-7. doi: 10.1073/pnas.1314576110. Epub 2013 Nov 11.


RNA-Seq reveals differential gene expression in Staphylococcus aureus with single-nucleotide resolution.

Osmundson J, Dewell S, Darst SA.

PLoS One. 2013 Oct 7;8(10):e76572. doi: 10.1371/journal.pone.0076572. eCollection 2013.


Crystallographic analysis of an RNA polymerase σ-subunit fragment complexed with -10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein-ssDNA complexes.

Feklistov A, Darst SA.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):950-5. doi: 10.1107/S1744309113020368. Epub 2013 Aug 19.


Marking and measuring single microtubules by PRC1 and kinesin-4.

Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM.

Cell. 2013 Jul 18;154(2):377-90. doi: 10.1016/j.cell.2013.06.021. Erratum in: Cell. 2013 Nov 21;155(5):1188.


Structure and function of CarD, an essential mycobacterial transcription factor.

Srivastava DB, Leon K, Osmundson J, Garner AL, Weiss LA, Westblade LF, Glickman MS, Landick R, Darst SA, Stallings CL, Campbell EA.

Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12619-24. doi: 10.1073/pnas.1308270110. Epub 2013 Jul 15.


Biochemical insights into the function of phage G1 gp67 in Staphylococcus aureus.

Osmundson J, Darst SA.

Bacteriophage. 2013 Jan 1;3(1):e24767.


Phage-encoded inhibitor of Staphylococcus aureus transcription exerts context-dependent effects on promoter function in a modified Escherichia coli-based transcription system.

Montero-Diez C, Deighan P, Osmundson J, Darst SA, Hochschild A.

J Bacteriol. 2013 Aug;195(16):3621-8. doi: 10.1128/JB.00499-13. Epub 2013 Jun 7.


Structural basis of transcriptional pausing in bacteria.

Weixlbaumer A, Leon K, Landick R, Darst SA.

Cell. 2013 Jan 31;152(3):431-41. doi: 10.1016/j.cell.2012.12.020.


Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.

Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA.

Cell. 2012 Nov 21;151(5):1005-16. doi: 10.1016/j.cell.2012.10.034.


Initiation of transcription-coupled repair characterized at single-molecule resolution.

Howan K, Smith AJ, Westblade LF, Joly N, Grange W, Zorman S, Darst SA, Savery NJ, Strick TR.

Nature. 2012 Oct 18;490(7420):431-4. doi: 10.1038/nature11430. Epub 2012 Sep 9.


Interaction of CarD with RNA polymerase mediates Mycobacterium tuberculosis viability, rifampin resistance, and pathogenesis.

Weiss LA, Harrison PG, Nickels BE, Glickman MS, Campbell EA, Darst SA, Stallings CL.

J Bacteriol. 2012 Oct;194(20):5621-31. Epub 2012 Aug 17.


Structural basis for promoter-10 element recognition by the bacterial RNA polymerase σ subunit.

Feklistov A, Darst SA.

Cell. 2011 Dec 9;147(6):1257-69. doi: 10.1016/j.cell.2011.10.041. Epub 2011 Dec 1.


Crystal structure of the bacteriophage T4 late-transcription coactivator gp33 with the β-subunit flap domain of Escherichia coli RNA polymerase.

Twist KA, Campbell EA, Deighan P, Nechaev S, Jain V, Geiduschek EP, Hochschild A, Darst SA.

Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19961-6. doi: 10.1073/pnas.1113328108. Epub 2011 Dec 1.


A novel method for the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking the α C-terminal domain.

Twist KA, Husnain SI, Franke JD, Jain D, Campbell EA, Nickels BE, Thomas MS, Darst SA, Westblade LF.

Protein Sci. 2011 Jun;20(6):986-95. doi: 10.1002/pro.622. Epub 2011 Apr 26.


Derepression of bacterial transcription-repair coupling factor is associated with a profound conformational change.

Srivastava DB, Darst SA.

J Mol Biol. 2011 Feb 18;406(2):275-84. doi: 10.1016/j.jmb.2010.12.004. Epub 2010 Dec 23.


The 2.7 Å resolution structure of the glycopeptide sulfotransferase Teg14.

Bick MJ, Banik JJ, Darst SA, Brady SF.

Acta Crystallogr D Biol Crystallogr. 2010 Dec;66(Pt 12):1278-86. doi: 10.1107/S0907444910036681. Epub 2010 Nov 16.


Temporal regulation of gene expression of the Thermus thermophilus bacteriophage P23-45.

Berdygulova Z, Westblade LF, Florens L, Koonin EV, Chait BT, Ramanculov E, Washburn MP, Darst SA, Severinov K, Minakhin L.

J Mol Biol. 2011 Jan 7;405(1):125-42. doi: 10.1016/j.jmb.2010.10.049. Epub 2010 Nov 2.


Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.

Opalka N, Brown J, Lane WJ, Twist KA, Landick R, Asturias FJ, Darst SA.

PLoS Biol. 2010 Sep 14;8(9). pii: e1000483. doi: 10.1371/journal.pbio.1000483.


Structural basis for the bacterial transcription-repair coupling factor/RNA polymerase interaction.

Westblade LF, Campbell EA, Pukhrambam C, Padovan JC, Nickels BE, Lamour V, Darst SA.

Nucleic Acids Res. 2010 Dec;38(22):8357-69. doi: 10.1093/nar/gkq692. Epub 2010 Aug 11.


Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.

Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM.

Cell. 2010 Aug 6;142(3):433-43. doi: 10.1016/j.cell.2010.07.012.


Promoter melting triggered by bacterial RNA polymerase occurs in three steps.

Chen J, Darst SA, Thirumalai D.

Proc Natl Acad Sci U S A. 2010 Jul 13;107(28):12523-8. doi: 10.1073/pnas.1003533107. Epub 2010 Jul 1.


Climate change and the integrity of science.

Gleick PH, Adams RM, Amasino RM, Anders E, Anderson DJ, Anderson WW, Anselin LE, Arroyo MK, Asfaw B, Ayala FJ, Bax A, Bebbington AJ, Bell G, Bennett MV, Bennetzen JL, Berenbaum MR, Berlin OB, Bjorkman PJ, Blackburn E, Blamont JE, Botchan MR, Boyer JS, Boyle EA, Branton D, Briggs SP, Briggs WR, Brill WJ, Britten RJ, Broecker WS, Brown JH, Brown PO, Brunger AT, Cairns J Jr, Canfield DE, Carpenter SR, Carrington JC, Cashmore AR, Castilla JC, Cazenave A, Chapin FS 3rd, Ciechanover AJ, Clapham DE, Clark WC, Clayton RN, Coe MD, Conwell EM, Cowling EB, Cowling RM, Cox CS, Croteau RB, Crothers DM, Crutzen PJ, Daily GC, Dalrymple GB, Dangl JL, Darst SA, Davies DR, Davis MB, De Camilli PV, Dean C, DeFries RS, Deisenhofer J, Delmer DP, DeLong EF, DeRosier DJ, Diener TO, Dirzo R, Dixon JE, Donoghue MJ, Doolittle RF, Dunne T, Ehrlich PR, Eisenstadt SN, Eisner T, Emanuel KA, Englander SW, Ernst WG, Falkowski PG, Feher G, Ferejohn JA, Fersht A, Fischer EH, Fischer R, Flannery KV, Frank J, Frey PA, Fridovich I, Frieden C, Futuyma DJ, Gardner WR, Garrett CJ, Gilbert W, Goldberg RB, Goodenough WH, Goodman CS, Goodman M, Greengard P, Hake S, Hammel G, Hanson S, Harrison SC, Hart SR, Hartl DL, Haselkorn R, Hawkes K, Hayes JM, Hille B, Hökfelt T, House JS, Hout M, Hunten DM, Izquierdo IA, Jagendorf AT, Janzen DH, Jeanloz R, Jencks CS, Jury WA, Kaback HR, Kailath T, Kay P, Kay SA, Kennedy D, Kerr A, Kessler RC, Khush GS, Kieffer SW, Kirch PV, Kirk K, Kivelson MG, Klinman JP, Klug A, Knopoff L, Kornberg H, Kutzbach JE, Lagarias JC, Lambeck K, Landy A, Langmuir CH, Larkins BA, Le Pichon XT, Lenski RE, Leopold EB, Levin SA, Levitt M, Likens GE, Lippincott-Schwartz J, Lorand L, Lovejoy CO, Lynch M, Mabogunje AL, Malone TF, Manabe S, Marcus J, Massey DS, McWilliams JC, Medina E, Melosh HJ, Meltzer DJ, Michener CD, Miles EL, Mooney HA, Moore PB, Morel FM, Mosley-Thompson ES, Moss B, Munk WH, Myers N, Nair GB, Nathans J, Nester EW, Nicoll RA, Novick RP, O'Connell JF, Olsen PE, Opdyke ND, Oster GF, Ostrom E, Pace NR, Paine RT, Palmiter RD, Pedlosky J, Petsko GA, Pettengill GH, Philander SG, Piperno DR, Pollard TD, Price PB Jr, Reichard PA, Reskin BF, Ricklefs RE, Rivest RL, Roberts JD, Romney AK, Rossmann MG, Russell DW, Rutter WJ, Sabloff JA, Sagdeev RZ, Sahlins MD, Salmond A, Sanes JR, Schekman R, Schellnhuber J, Schindler DW, Schmitt J, Schneider SH, Schramm VL, Sederoff RR, Shatz CJ, Sherman F, Sidman RL, Sieh K, Simons EL, Singer BH, Singer MF, Skyrms B, Sleep NH, Smith BD, Snyder SH, Sokal RR, Spencer CS, Steitz TA, Strier KB, Südhof TC, Taylor SS, Terborgh J, Thomas DH, Thompson LG, Tjian RT, Turner MG, Uyeda S, Valentine JW, Valentine JS, Van Etten JL, van Holde KE, Vaughan M, Verba S, von Hippel PH, Wake DB, Walker A, Walker JE, Watson EB, Watson PJ, Weigel D, Wessler SR, West-Eberhard MJ, White TD, Wilson WJ, Wolfenden RV, Wood JA, Woodwell GM, Wright HE Jr, Wu C, Wunsch C, Zoback ML.

Science. 2010 May 7;328(5979):689-90. doi: 10.1126/science.328.5979.689. No abstract available. Erratum in: Science. 2010 May 14;328(5980):826.


Crystal structures of the glycopeptide sulfotransferase Teg12 in a complex with the teicoplanin aglycone.

Bick MJ, Banik JJ, Darst SA, Brady SF.

Biochemistry. 2010 May 18;49(19):4159-68. doi: 10.1021/bi100150v.

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