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Items: 24


The complete genome sequence of the nitrile biocatalyst Rhodocccus rhodochrous ATCC BAA-870.

Frederick J, Hennessy F, Horn U, de la Torre Cortés P, van den Broek M, Strych U, Willson R, Hefer CA, Daran JG, Sewell T, Otten LG, Brady D.

BMC Genomics. 2020 Jan 2;21(1):3. doi: 10.1186/s12864-019-6405-7.


Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization.

Salazar AN, Gorter de Vries AR, van den Broek M, Brouwers N, de la Torre Cortès P, Kuijpers NGA, Daran JG, Abeel T.

BMC Genomics. 2019 Dec 2;20(1):916. doi: 10.1186/s12864-019-6263-3.


Connecting central carbon and aromatic amino acid metabolisms to improve de novo 2-phenylethanol production in Saccharomyces cerevisiae.

Hassing EJ, de Groot PA, Marquenie VR, Pronk JT, Daran JG.

Metab Eng. 2019 Dec;56:165-180. doi: 10.1016/j.ymben.2019.09.011. Epub 2019 Sep 28.


Lager-brewing yeasts in the era of modern genetics.

Gorter de Vries AR, Pronk JT, Daran JG.

FEMS Yeast Res. 2019 Nov 1;19(7). pii: foz063. doi: 10.1093/femsyr/foz063.


Himalayan Saccharomyces eubayanus Genome Sequences Reveal Genetic Markers Explaining Heterotic Maltotriose Consumption by Saccharomyces pastorianus Hybrids.

Brouwers N, Brickwedde A, Gorter de Vries AR, van den Broek M, Weening SM, van den Eijnden L, Diderich JA, Bai FY, Pronk JT, Daran JG.

Appl Environ Microbiol. 2019 Oct 30;85(22). pii: e01516-19. doi: 10.1128/AEM.01516-19. Print 2019 Nov 15.


Biological Parts for Kluyveromyces marxianus Synthetic Biology.

Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP.

Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 10.3389/fbioe.2019.00097. eCollection 2019.


Phenotype-Independent Isolation of Interspecies Saccharomyces Hybrids by Dual-Dye Fluorescent Staining and Fluorescence-Activated Cell Sorting.

Gorter de Vries AR, Koster CC, Weening SM, Luttik MAH, Kuijpers NGA, Geertman JA, Pronk JT, Daran JG.

Front Microbiol. 2019 Apr 26;10:871. doi: 10.3389/fmicb.2019.00871. eCollection 2019.


Laboratory Evolution of a Saccharomyces cerevisiae × S. eubayanus Hybrid Under Simulated Lager-Brewing Conditions.

Gorter de Vries AR, Voskamp MA, van Aalst ACA, Kristensen LH, Jansen L, van den Broek M, Salazar AN, Brouwers N, Abeel T, Pronk JT, Daran JG.

Front Genet. 2019 Mar 29;10:242. doi: 10.3389/fgene.2019.00242. eCollection 2019.


In vivo recombination of Saccharomyces eubayanus maltose-transporter genes yields a chimeric transporter that enables maltotriose fermentation.

Brouwers N, Gorter de Vries AR, van den Broek M, Weening SM, Elink Schuurman TD, Kuijpers NGA, Pronk JT, Daran JG.

PLoS Genet. 2019 Apr 4;15(4):e1007853. doi: 10.1371/journal.pgen.1007853. eCollection 2019 Apr.


Allele-specific genome editing using CRISPR-Cas9 is associated with loss of heterozygosity in diploid yeast.

Gorter de Vries AR, Couwenberg LGF, van den Broek M, de la Torre Cortés P, Ter Horst J, Pronk JT, Daran JG.

Nucleic Acids Res. 2019 Feb 20;47(3):1362-1372. doi: 10.1093/nar/gky1216.


Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes in Saccharomyces eubayanus CBS 12357T.

Brickwedde A, Brouwers N, van den Broek M, Gallego Murillo JS, Fraiture JL, Pronk JT, Daran JG.

Front Microbiol. 2018 Aug 10;9:1786. doi: 10.3389/fmicb.2018.01786. eCollection 2018.


Selection of Pof-Saccharomyces eubayanus Variants for the Construction of S. cerevisiae × S. eubayanus Hybrids With Reduced 4-Vinyl Guaiacol Formation.

Diderich JA, Weening SM, van den Broek M, Pronk JT, Daran JG.

Front Microbiol. 2018 Jul 27;9:1640. doi: 10.3389/fmicb.2018.01640. eCollection 2018.


Fermentation of glucose-xylose-arabinose mixtures by a synthetic consortium of single-sugar-fermenting Saccharomyces cerevisiae strains.

Verhoeven MD, de Valk SC, Daran JG, van Maris AJA, Pronk JT.

FEMS Yeast Res. 2018 Dec 1;18(8). doi: 10.1093/femsyr/foy075.


Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive l-arabinose uptake.

Verhoeven MD, Bracher JM, Nijland JG, Bouwknegt J, Daran JG, Driessen AJM, van Maris AJA, Pronk JT.

FEMS Yeast Res. 2018 Sep 1;18(6). doi: 10.1093/femsyr/foy062.


The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive l-arabinose transport in Saccharomyces cerevisiae.

Bracher JM, Verhoeven MD, Wisselink HW, Crimi B, Nijland JG, Driessen AJM, Klaassen P, van Maris AJA, Daran JG, Pronk JT.

Biotechnol Biofuels. 2018 Mar 13;11:63. doi: 10.1186/s13068-018-1047-6. eCollection 2018.


Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid.

Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG.

FEMS Yeast Res. 2018 May 1;18(3). doi: 10.1093/femsyr/foy012.


CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.

Gorter de Vries AR, de Groot PA, van den Broek M, Daran JG.

Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1.


Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production.

Mans R, Daran JG, Pronk JT.

Curr Opin Biotechnol. 2018 Apr;50:47-56. doi: 10.1016/j.copbio.2017.10.011. Epub 2017 Nov 20. Review.


Nanopore sequencing enables near-complete de novo assembly of Saccharomyces cerevisiae reference strain CEN.PK113-7D.

Salazar AN, Gorter de Vries AR, van den Broek M, Wijsman M, de la Torre Cortés P, Brickwedde A, Brouwers N, Daran JG, Abeel T.

FEMS Yeast Res. 2017 Nov 1;17(7). doi: 10.1093/femsyr/fox074.


Evolutionary Engineering in Chemostat Cultures for Improved Maltotriose Fermentation Kinetics in Saccharomyces pastorianus Lager Brewing Yeast.

Brickwedde A, van den Broek M, Geertman JA, Magalhães F, Kuijpers NGA, Gibson B, Pronk JT, Daran JG.

Front Microbiol. 2017 Sep 8;8:1690. doi: 10.3389/fmicb.2017.01690. eCollection 2017.


Laboratory Evolution of a Biotin-Requiring Saccharomyces cerevisiae Strain for Full Biotin Prototrophy and Identification of Causal Mutations.

Bracher JM, de Hulster E, Koster CC, van den Broek M, Daran JG, van Maris AJA, Pronk JT.

Appl Environ Microbiol. 2017 Aug 1;83(16). pii: e00892-17. doi: 10.1128/AEM.00892-17. Print 2017 Aug 15.


Mutations in PMR1 stimulate xylose isomerase activity and anaerobic growth on xylose of engineered Saccharomyces cerevisiae by influencing manganese homeostasis.

Verhoeven MD, Lee M, Kamoen L, van den Broek M, Janssen DB, Daran JG, van Maris AJ, Pronk JT.

Sci Rep. 2017 Apr 12;7:46155. doi: 10.1038/srep46155.


Industrial Relevance of Chromosomal Copy Number Variation in Saccharomyces Yeasts.

Gorter de Vries AR, Pronk JT, Daran JG.

Appl Environ Microbiol. 2017 May 17;83(11). pii: e03206-16. doi: 10.1128/AEM.03206-16. Print 2017 Jun 1. Review.


Elimination of sucrose transport and hydrolysis in Saccharomyces cerevisiae: a platform strain for engineering sucrose metabolism.

Marques WL, Mans R, Marella ER, Cordeiro RL, van den Broek M, Daran JG, Pronk JT, Gombert AK, van Maris AJ.

FEMS Yeast Res. 2017 Jan 1;17(1). doi: 10.1093/femsyr/fox006.

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