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J Cell Sci. 2019 Sep 6;132(17). pii: jcs230672. doi: 10.1242/jcs.230672.

The golgin PpImh1 mediates reversible cisternal stacking in the Golgi of the budding yeast Pichia pastoris.

Author information

1
Department of Cell and Tumor Biology, Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210 MH, India.
2
Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, MH 400085, India.
3
Department of Cell and Tumor Biology, Advanced Centre for Treatment Research & Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210 MH, India dbhattacharyya@actrec.gov.in.

Abstract

The adhesive force for cisternal stacking of Golgi needs to be reversible - to be initiated and undone in a continuous cycle to keep up with the cisternal maturation. Microscopic evidence in support of such a reversible nature of stacking, in the form of 'TGN peeling,' has been reported in various species, suggesting a potential evolutionarily conserved mechanism. However, knowledge of such mechanism has remained sketchy. Here, we have explored this issue in the budding yeast Pichia pastoris which harbors stacked Golgi. We observed that deletion of GRIP domain golgin P. pastoris (Pp)IMH1 increases the peeling of late cisterna, causing unstacking of the Golgi stack. Our results suggest that the PpImh1 dimer mediates reversible stacking through a continuous association-dissociation cycle of its GRIP domain to the middle and late Golgi cisterna under the GTP hydrolysis-based regulation of Arl3-Arl1 GTPase cascade switch. The reversible cisternal stacking function of PpImh1 is independent of its vesicle-capturing function. Since GRIP domain proteins are conserved in plants, animals and fungi, it is plausible that this reversible mechanism of Golgi stacking is evolutionarily conserved.This article has an associated First Person interview with the first author of the paper.

KEYWORDS:

Cisternal stacking; GRIP domain; Golgi; Golgin; PpImh1

PMID:
31391238
DOI:
10.1242/jcs.230672

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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