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Characterization of six recombinant human RNase H2 bearing Aicardi-Goutiéres syndrome causing mutations.

Nishimura T, Baba M, Ogawa S, Kojima K, Takita T, Crouch RJ, Yasukawa K.

J Biochem. 2019 Sep 16. pii: mvz073. doi: 10.1093/jb/mvz073. [Epub ahead of print]


Unlike the Escherichia coli counterpart, archaeal RNase HII cannot process ribose monophosphate abasic sites and oxidized ribonucleotides embedded in DNA.

Malfatti MC, Henneke G, Balachander S, Koh KD, Newnam G, Uehara R, Crouch RJ, Storici F, Tell G.

J Biol Chem. 2019 Aug 30;294(35):13061-13072. doi: 10.1074/jbc.RA119.009493. Epub 2019 Jul 12.


Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.

Deasy SK, Uehara R, Vodnala SK, Yang HH, Dass RA, Hu Y, Lee MP, Crouch RJ, Hunter KW.

PLoS Genet. 2019 May 24;15(5):e1008020. doi: 10.1371/journal.pgen.1008020. eCollection 2019 May.


Two RNase H2 Mutants with Differential rNMP Processing Activity Reveal a Threshold of Ribonucleotide Tolerance for Embryonic Development.

Uehara R, Cerritelli SM, Hasin N, Sakhuja K, London M, Iranzo J, Chon H, Grinberg A, Crouch RJ.

Cell Rep. 2018 Oct 30;25(5):1135-1145.e5. doi: 10.1016/j.celrep.2018.10.019.


Abasic and oxidized ribonucleotides embedded in DNA are processed by human APE1 and not by RNase H2.

Malfatti MC, Balachander S, Antoniali G, Koh KD, Saint-Pierre C, Gasparutto D, Chon H, Crouch RJ, Storici F, Tell G.

Nucleic Acids Res. 2017 Nov 2;45(19):11193-11212. doi: 10.1093/nar/gkx723.


Effects of neutral salts and pH on the activity and stability of human RNase H2.

Baba M, Kojima K, Nakase R, Imai S, Yamasaki T, Takita T, Crouch RJ, Yasukawa K.

J Biochem. 2017 Sep 1;162(3):211-219. doi: 10.1093/jb/mvx021.


R-Loop Depletion by Over-expressed RNase H1 in Mouse B Cells Increases Activation-Induced Deaminase Access to the Transcribed Strand without Altering Frequency of Isotype Switching.

Maul RW, Chon H, Sakhuja K, Cerritelli SM, Gugliotti LA, Gearhart PJ, Crouch RJ.

J Mol Biol. 2017 Oct 27;429(21):3255-3263. doi: 10.1016/j.jmb.2016.12.020. Epub 2017 Jan 6.


Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.

Akman G, Desai R, Bailey LJ, Yasukawa T, Dalla Rosa I, Durigon R, Holmes JB, Moss CF, Mennuni M, Houlden H, Crouch RJ, Hanna MG, Pitceathly RD, Spinazzola A, Holt IJ.

Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4276-85. doi: 10.1073/pnas.1600537113. Epub 2016 Jul 8.


The Balancing Act of Ribonucleotides in DNA.

Cerritelli SM, Crouch RJ.

Trends Biochem Sci. 2016 May;41(5):434-445. doi: 10.1016/j.tibs.2016.02.005. Epub 2016 Mar 17. Review.


RNase H2 catalytic core Aicardi-Goutières syndrome-related mutant invokes cGAS-STING innate immune-sensing pathway in mice.

Pokatayev V, Hasin N, Chon H, Cerritelli SM, Sakhuja K, Ward JM, Morris HD, Yan N, Crouch RJ.

J Exp Med. 2016 Mar 7;213(3):329-36. doi: 10.1084/jem.20151464. Epub 2016 Feb 15.


Unlocking the steric gate of DNA polymerase η leads to increased genomic instability in Saccharomyces cerevisiae.

Donigan KA, Cerritelli SM, McDonald JP, Vaisman A, Crouch RJ, Woodgate R.

DNA Repair (Amst). 2015 Nov;35:1-12. doi: 10.1016/j.dnarep.2015.07.002. Epub 2015 Aug 7.


Primer retention owing to the absence of RNase H1 is catastrophic for mitochondrial DNA replication.

Holmes JB, Akman G, Wood SR, Sakhuja K, Cerritelli SM, Moss C, Bowmaker MR, Jacobs HT, Crouch RJ, Holt IJ.

Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):9334-9. doi: 10.1073/pnas.1503653112. Epub 2015 Jul 10.


Targeted deletion of the gene encoding the La autoantigen (Sjögren's syndrome antigen B) in B cells or the frontal brain causes extensive tissue loss.

Gaidamakov S, Maximova OA, Chon H, Blewett NH, Wang H, Crawford AK, Day A, Tulchin N, Crouch RJ, Morse HC 3rd, Blitzer RD, Maraia RJ.

Mol Cell Biol. 2014 Jan;34(1):123-31. doi: 10.1128/MCB.01010-13. Epub 2013 Nov 4.


RNase H2 roles in genome integrity revealed by unlinking its activities.

Chon H, Sparks JL, Rychlik M, Nowotny M, Burgers PM, Crouch RJ, Cerritelli SM.

Nucleic Acids Res. 2013 Mar 1;41(5):3130-43. doi: 10.1093/nar/gkt027. Epub 2013 Jan 25.


RNase H2-initiated ribonucleotide excision repair.

Sparks JL, Chon H, Cerritelli SM, Kunkel TA, Johansson E, Crouch RJ, Burgers PM.

Mol Cell. 2012 Sep 28;47(6):980-6. doi: 10.1016/j.molcel.2012.06.035. Epub 2012 Aug 2.


Molecular biology. A new twist for topoisomerase.

Cerritelli SM, Chon H, Crouch RJ.

Science. 2011 Jun 24;332(6037):1510-1. doi: 10.1126/science.1208450. No abstract available.


Actin and myosin contribute to mammalian mitochondrial DNA maintenance.

Reyes A, He J, Mao CC, Bailey LJ, Di Re M, Sembongi H, Kazak L, Dzionek K, Holmes JB, Cluett TJ, Harbour ME, Fearnley IM, Crouch RJ, Conti MA, Adelstein RS, Walker JE, Holt IJ.

Nucleic Acids Res. 2011 Jul;39(12):5098-108. doi: 10.1093/nar/gkr052. Epub 2011 Mar 11.


The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutières syndrome defects.

Figiel M, Chon H, Cerritelli SM, Cybulska M, Crouch RJ, Nowotny M.

J Biol Chem. 2011 Mar 25;286(12):10540-50. doi: 10.1074/jbc.M110.181974. Epub 2010 Dec 22.


Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage.

Rychlik MP, Chon H, Cerritelli SM, Klimek P, Crouch RJ, Nowotny M.

Mol Cell. 2010 Nov 24;40(4):658-70. doi: 10.1016/j.molcel.2010.11.001.


An upstream open reading frame and the context of the two AUG codons affect the abundance of mitochondrial and nuclear RNase H1.

Suzuki Y, Holmes JB, Cerritelli SM, Sakhuja K, Minczuk M, Holt IJ, Crouch RJ.

Mol Cell Biol. 2010 Nov;30(21):5123-34. doi: 10.1128/MCB.00619-10. Epub 2010 Sep 7.


Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.

Pohjoismäki JL, Holmes JB, Wood SR, Yang MY, Yasukawa T, Reyes A, Bailey LJ, Cluett TJ, Goffart S, Willcox S, Rigby RE, Jackson AP, Spelbrink JN, Griffith JD, Crouch RJ, Jacobs HT, Holt IJ.

J Mol Biol. 2010 Apr 16;397(5):1144-55. doi: 10.1016/j.jmb.2010.02.029. Epub 2010 Feb 23.


Ribonuclease H: the enzymes in eukaryotes.

Cerritelli SM, Crouch RJ.

FEBS J. 2009 Mar;276(6):1494-505. doi: 10.1111/j.1742-4658.2009.06908.x. Epub 2008 Feb 18. Review.


Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex.

Chon H, Vassilev A, DePamphilis ML, Zhao Y, Zhang J, Burgers PM, Crouch RJ, Cerritelli SM.

Nucleic Acids Res. 2009 Jan;37(1):96-110. doi: 10.1093/nar/gkn913. Epub 2008 Nov 16.


Effect of the disease-causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII.

Rohman MS, Koga Y, Takano K, Chon H, Crouch RJ, Kanaya S.

FEBS J. 2008 Oct;275(19):4836-49. doi: 10.1111/j.1742-4658.2008.06622.x. Epub 2008 Aug 21.


Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD.

Nowotny M, Cerritelli SM, Ghirlando R, Gaidamakov SA, Crouch RJ, Yang W.

EMBO J. 2008 Apr 9;27(7):1172-81. doi: 10.1038/emboj.2008.44. Epub 2008 Mar 13.


Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription.

Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W.

Mol Cell. 2007 Oct 26;28(2):264-76. Erratum in: Mol Cell. 2007 Nov 9;28(3):513.


Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis.

Nowotny M, Gaidamakov SA, Crouch RJ, Yang W.

Cell. 2005 Jul 1;121(7):1005-16.


Eukaryotic RNases H1 act processively by interactions through the duplex RNA-binding domain.

Gaidamakov SA, Gorshkova II, Schuck P, Steinbach PJ, Yamada H, Crouch RJ, Cerritelli SM.

Nucleic Acids Res. 2005 Apr 14;33(7):2166-75. Print 2005.


Selective inhibition of HIV-1 reverse transcriptase-associated ribonuclease H activity by hydroxylated tropolones.

Budihas SR, Gorshkova I, Gaidamakov S, Wamiru A, Bona MK, Parniak MA, Crouch RJ, McMahon JB, Beutler JA, Le Grice SF.

Nucleic Acids Res. 2005 Mar 1;33(4):1249-56. Print 2005.


A capillary electrophoretic assay for ribonuclease H activity.

Chan KC, Budihas SR, Le Grice SF, Parniak MA, Crouch RJ, Gaidamakov SA, Isaaq HJ, Wamiru A, McMahon JB, Beutler JA.

Anal Biochem. 2004 Aug 15;331(2):296-302.


Effects of RNA polymerase modifications on transcription-induced negative supercoiling and associated R-loop formation.

Broccoli S, Rallu F, Sanscartier P, Cerritelli SM, Crouch RJ, Drolet M.

Mol Microbiol. 2004 Jun;52(6):1769-79.


RNase H2 of Saccharomyces cerevisiae is a complex of three proteins.

Jeong HS, Backlund PS, Chen HC, Karavanov AA, Crouch RJ.

Nucleic Acids Res. 2004 Jan 20;32(2):407-14. Print 2004. Erratum in: Nucleic Acids Res. 2004 Feb 24;32(4):1616.


Selective inhibitory DNA aptamers of the human RNase H1.

Pileur F, Andreola ML, Dausse E, Michel J, Moreau S, Yamada H, Gaidamakov SA, Crouch RJ, Toulmé JJ, Cazenave C.

Nucleic Acids Res. 2003 Oct 1;31(19):5776-88.


Humanized docking system for assembly of targeting drug delivery complexes.

Backer MV, Gaynutdinov TI, Gorshkova II, Crouch RJ, Hu T, Aloise R, Arab M, Przekop K, Backer JM.

J Control Release. 2003 May 20;89(3):499-511.


Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice.

Cerritelli SM, Frolova EG, Feng C, Grinberg A, Love PE, Crouch RJ.

Mol Cell. 2003 Mar;11(3):807-15.


Multiple ribonuclease H-encoding genes in the Caenorhabditis elegans genome contrasts with the two typical ribonuclease H-encoding genes in the human genome.

Arudchandran A, Cerritelli SM, Bowen NJ, Chen X, Krause MW, Crouch RJ.

Mol Biol Evol. 2002 Nov;19(11):1910-9.


RNase H1 of Saccharomyces cerevisiae: methods and nomenclature.

Crouch RJ, Arudchandran A, Cerritelli SM.

Methods Enzymol. 2001;341:395-413. No abstract available.


Quantitative regulation of class switch recombination by switch region transcription.

Lee CG, Kinoshita K, Arudchandran A, Cerritelli SM, Crouch RJ, Honjo T.

J Exp Med. 2001 Aug 6;194(3):365-74.


HIV-1 reverse transcriptase interaction with model RNA-DNA duplexes.

Gorshkova II, Rausch JW, Le Grice SF, Crouch RJ.

Anal Biochem. 2001 Apr 15;291(2):198-206.


Sorting of mannose 6-phosphate receptors mediated by the GGAs.

Puertollano R, Aguilar RC, Gorshkova I, Crouch RJ, Bonifacino JS.

Science. 2001 Jun 1;292(5522):1712-6.


Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4.

Aguilar RC, Boehm M, Gorshkova I, Crouch RJ, Tomita K, Saito T, Ohno H, Bonifacino JS.

J Biol Chem. 2001 Apr 20;276(16):13145-52. Epub 2001 Jan 3.


Detection of nucleic acid interactions using surface plasmon resonance.

Crouch RJ, Wakasa M, Haruki M.

Methods Mol Biol. 1999;118:143-60. No abstract available.


Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.

Itaya M, Omori A, Kanaya S, Crouch RJ, Tanaka T, Kondo K.

J Bacteriol. 1999 Apr;181(7):2118-23.


A common 40 amino acid motif in eukaryotic RNases H1 and caulimovirus ORF VI proteins binds to duplex RNAs.

Cerritelli SM, Fedoroff OY, Reid BR, Crouch RJ.

Nucleic Acids Res. 1998 Apr 1;26(7):1834-40.


Ribonuclease H renaturation gel assay using a fluorescent-labeled substrate.

Han LY, Ma WP, Crouch RJ.

Biotechniques. 1997 Nov;23(5):920-6.


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