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Items: 1 to 50 of 158

1.

Human Proteome Project Mass Spectrometry Data Interpretation Guidelines 3.0.

Deutsch EW, Lane L, Overall CM, Bandeira N, Baker MS, Pineau C, Moritz RL, Corrales F, Orchard S, Van Eyk JE, Paik YK, Weintraub ST, Vandenbrouck Y, Omenn GS.

J Proteome Res. 2019 Oct 10. doi: 10.1021/acs.jproteome.9b00542. [Epub ahead of print]

PMID:
31599596
2.

Blockade of the trans-sulfuration pathway in acute pancreatitis due to nitration of cystathionine β-synthase.

Rius-Pérez S, Pérez S, Torres-Cuevas I, Martí-Andrés P, Taléns-Visconti R, Paradela A, Guerrero L, Franco L, López-Rodas G, Torres L, Corrales F, Sastre J.

Redox Biol. 2019 Sep 8;28:101324. doi: 10.1016/j.redox.2019.101324. [Epub ahead of print]

3.

The Human Brain Proteome Project: Biological and Technological Challenges.

Fernández-Irigoyen J, Corrales F, Santamaría E.

Methods Mol Biol. 2019;2044:3-23. doi: 10.1007/978-1-4939-9706-0_1.

PMID:
31432403
4.

Progress on Identifying and Characterizing the Human Proteome: 2019 Metrics from the HUPO Human Proteome Project.

Omenn GS, Lane L, Overall CM, Corrales FJ, Schwenk JM, Paik YK, Van Eyk JE, Liu S, Pennington S, Snyder MP, Baker MS, Deutsch EW.

J Proteome Res. 2019 Sep 13. doi: 10.1021/acs.jproteome.9b00434. [Epub ahead of print]

PMID:
31430157
5.

Getting insights into hepatocellular carcinoma tumour heterogeneity by multiomics dissection.

Sarobe P, Corrales F.

Gut. 2019 Nov;68(11):1913-1914. doi: 10.1136/gutjnl-2019-319410. Epub 2019 Aug 2. No abstract available.

PMID:
31375598
6.

Toward Completion of the Human Proteome Parts List: Progress Uncovering Proteins That Are Missing or Have Unknown Function and Developing Analytical Methods.

Paik YK, Overall CM, Corrales F, Deutsch EW, Lane L, Omenn GS.

J Proteome Res. 2018 Dec 7;17(12):4023-4030. doi: 10.1021/acs.jproteome.8b00885. No abstract available.

7.

Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers.

Navajas R, Corrales FJ, Paradela A.

Methods Mol Biol. 2019;1959:39-50. doi: 10.1007/978-1-4939-9164-8_3.

PMID:
30852814
8.

Launching the C-HPP neXt-CP50 Pilot Project for Functional Characterization of Identified Proteins with No Known Function.

Paik YK, Lane L, Kawamura T, Chen YJ, Cho JY, LaBaer J, Yoo JS, Domont G, Corrales F, Omenn GS, Archakov A, Encarnación-Guevara S, Lui S, Salekdeh GH, Cho JY, Kim CY, Overall CM.

J Proteome Res. 2018 Dec 7;17(12):4042-4050. doi: 10.1021/acs.jproteome.8b00383. Epub 2018 Nov 29.

9.

Liver cancer-associated changes to the proteome: what deserves clinical focus?

Mato JM, Elortza F, Lu SC, Brun V, Paradela A, Corrales FJ.

Expert Rev Proteomics. 2018 Sep;15(9):749-756. doi: 10.1080/14789450.2018.1521277. Epub 2018 Sep 19. Review.

PMID:
30204005
10.

Progress on Identifying and Characterizing the Human Proteome: 2018 Metrics from the HUPO Human Proteome Project.

Omenn GS, Lane L, Overall CM, Corrales FJ, Schwenk JM, Paik YK, Van Eyk JE, Liu S, Snyder M, Baker MS, Deutsch EW.

J Proteome Res. 2018 Dec 7;17(12):4031-4041. doi: 10.1021/acs.jproteome.8b00441. Epub 2018 Aug 23.

11.

Bioengineered PBGD variant improves the therapeutic index of gene therapy vectors for acute intermittent porphyria.

Serrano-Mendioroz I, Sampedro A, Serna N, de Salamanca RE, Sanz-Parra A, Corrales F, Berraondo P, Millet O, Fontanellas A.

Hum Mol Genet. 2018 Nov 1;27(21):3688-3696. doi: 10.1093/hmg/ddy283.

PMID:
30085095
12.

Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.

Clemente LF, Hernáez ML, Ramos-Fernández A, Ligero G, Gil C, Corrales FJ, Marcilla M.

J Proteome Res. 2018 Dec 7;17(12):4325-4328. doi: 10.1021/acs.jproteome.8b00384. Epub 2018 Jul 6.

PMID:
29974749
13.

Interaction between an ADCY3 Genetic Variant and Two Weight-Lowering Diets Affecting Body Fatness and Body Composition Outcomes Depending on Macronutrient Distribution: A Randomized Trial.

Goni L, Riezu-Boj JI, Milagro FI, Corrales FJ, Ortiz L, Cuervo M, Martínez JA.

Nutrients. 2018 Jun 19;10(6). pii: E789. doi: 10.3390/nu10060789.

14.

In-Depth Proteomic Characterization of Classical and Non-Classical Monocyte Subsets.

Segura V, Valero ML, Cantero L, Muñoz J, Zarzuela E, García F, Aloria K, Beaskoetxea J, Arizmendi JM, Navajas R, Paradela A, Díez P, Dégano RM, Fuentes M, Orfao A, Montero AG, Garin-Muga A, Corrales FJ, Pino MMSD.

Proteomes. 2018 Feb 5;6(1). pii: E8. doi: 10.3390/proteomes6010008.

15.

Engineered fibroblast growth factor 19 protects from acetaminophen-induced liver injury and stimulates aged liver regeneration in mice.

Alvarez-Sola G, Uriarte I, Latasa MU, Jimenez M, Barcena-Varela M, Santamaría E, Urtasun R, Rodriguez-Ortigosa C, Prieto J, Corrales FJ, Baulies A, García-Ruiz C, Fernandez-Checa JC, Berraondo P, Fernandez-Barrena MG, Berasain C, Avila MA.

Cell Death Dis. 2017 Oct 5;8(10):e3083. doi: 10.1038/cddis.2017.480.

16.

Enhanced Missing Proteins Detection in NCI60 Cell Lines Using an Integrative Search Engine Approach.

Guruceaga E, Garin-Muga A, Prieto G, Bejarano B, Marcilla M, Marín-Vicente C, Perez-Riverol Y, Casal JI, Vizcaíno JA, Corrales FJ, Segura V.

J Proteome Res. 2017 Dec 1;16(12):4374-4390. doi: 10.1021/acs.jproteome.7b00388. Epub 2017 Oct 11.

17.

Prioritizing Popular Proteins in Liver Cancer: Remodelling One-Carbon Metabolism.

Mora MI, Molina M, Odriozola L, Elortza F, Mato JM, Sitek B, Zhang P, He F, Latasa MU, Ávila MA, Corrales FJ.

J Proteome Res. 2017 Dec 1;16(12):4506-4514. doi: 10.1021/acs.jproteome.7b00390. Epub 2017 Oct 2.

PMID:
28944671
18.

A new mass spectrometry-based method for the quantification of histones in plasma from septic shock patients.

García-Giménez JL, Romá-Mateo C, Carbonell N, Palacios L, Peiró-Chova L, García-López E, García-Simón M, Lahuerta R, Gimenez-Garzó C, Berenguer-Pascual E, Mora MI, Valero ML, Alpízar A, Corrales FJ, Blanquer J, Pallardó FV.

Sci Rep. 2017 Sep 6;7(1):10643. doi: 10.1038/s41598-017-10830-z.

19.

A community proposal to integrate proteomics activities in ELIXIR.

Vizcaíno JA, Walzer M, Jiménez RC, Bittremieux W, Bouyssié D, Carapito C, Corrales F, Ferro M, Heck AJR, Horvatovich P, Hubalek M, Lane L, Laukens K, Levander F, Lisacek F, Novak P, Palmblad M, Piovesan D, Pühler A, Schwämmle V, Valkenborg D, van Rijswijk M, Vondrasek J, Eisenacher M, Martens L, Kohlbacher O.

F1000Res. 2017 Jun 13;6. pii: ELIXIR-875. doi: 10.12688/f1000research.11751.1. eCollection 2017.

20.

Fibroblast Growth Factor 15/19 in Hepatocarcinogenesis.

Alvarez-Sola G, Uriarte I, Latasa MU, Urtasun R, Bárcena-Varela M, Elizalde M, Jiménez M, Rodriguez-Ortigosa CM, Corrales FJ, Fernández-Barrena MG, Berasain C, Avila MA.

Dig Dis. 2017;35(3):158-165. doi: 10.1159/000450905. Epub 2017 Mar 1. Review.

PMID:
28249259
21.

Localisation of stem cell factor, stanniocalcin-1, connective tissue growth factor and heparin-binding epidermal growth factor in the bovine uterus at the time of blastocyst formation.

Muñoz M, Martin D, Carrocera S, Alonso-Guervos M, Mora MI, Corrales FJ, Peynot N, Giraud-Delville C, Duranthon V, Sandra O, Gómez E.

Reprod Fertil Dev. 2017 Oct;29(11):2127-2139. doi: 10.1071/RD16383.

PMID:
28231867
22.

Maresin 1 improves insulin sensitivity and attenuates adipose tissue inflammation in ob/ob and diet-induced obese mice.

Martínez-Fernández L, González-Muniesa P, Laiglesia LM, Sáinz N, Prieto-Hontoria PL, Escoté X, Odriozola L, Corrales FJ, Arbones-Mainar JM, Martínez JA, Moreno-Aliaga MJ.

FASEB J. 2017 May;31(5):2135-2145. doi: 10.1096/fj.201600859R. Epub 2017 Feb 10.

PMID:
28188173
23.

Fibroblast growth factor 15/19 (FGF15/19) protects from diet-induced hepatic steatosis: development of an FGF19-based chimeric molecule to promote fatty liver regeneration.

Alvarez-Sola G, Uriarte I, Latasa MU, Fernandez-Barrena MG, Urtasun R, Elizalde M, Barcena-Varela M, Jiménez M, Chang HC, Barbero R, Catalán V, Rodríguez A, Frühbeck G, Gallego-Escuredo JM, Gavaldà-Navarro A, Villarroya F, Rodriguez-Ortigosa CM, Corrales FJ, Prieto J, Berraondo P, Berasain C, Avila MA.

Gut. 2017 Oct;66(10):1818-1828. doi: 10.1136/gutjnl-2016-312975. Epub 2017 Jan 24.

PMID:
28119353
24.

A multicentric study to evaluate the use of relative retention times in targeted proteomics.

Vialas V, Colomé-Calls N, Abian J, Aloria K, Alvarez-Llamas G, Antúnez O, Arizmendi JM, Azkargorta M, Barceló-Batllori S, Barderas MG, Blanco F, Casal JI, Casas V, de la Torre C, Chicano-Gálvez E, Elortza F, Espadas G, Estanyol JM, Fernandez-Irigoyen J, Fernandez-Puente P, Fidalgo MJ, Fuentes M, Gay M, Gil C, Hainard A, Hernaez ML, Ibarrola N, Kopylov AT, Lario A, Lopez JA, López-Lucendo M, Marcilla M, Marina-Ramírez A, Marko-Varga G, Martín L, Mora MI, Morato-López E, Muñoz J, Odena MA, de Oliveira E, Orera I, Ortea I, Pasquarello C, Ray KB, Rezeli M, Ruppen I, Sabidó E, Del Pino MMS, Sancho J, Santamaría E, Vazquez J, Vilaseca M, Vivanco F, Walters JJ, Zgoda VG, Corrales FJ, Canals F, Paradela A.

J Proteomics. 2017 Jan 30;152:138-149. doi: 10.1016/j.jprot.2016.10.014. Epub 2016 Oct 29.

PMID:
27989941
25.

Progress and pitfalls in finding the 'missing proteins' from the human proteome map.

Segura V, Garin-Muga A, Guruceaga E, Corrales FJ.

Expert Rev Proteomics. 2017 Jan;14(1):9-14. doi: 10.1080/14789450.2017.1265450. Epub 2016 Dec 2. Review.

PMID:
27885863
26.

Understanding the Functional Plasticity in Neural Networks of the Basal Ganglia in Cocaine Use Disorder: A Role for Allosteric Receptor-Receptor Interactions in A2A-D2 Heteroreceptor Complexes.

Borroto-Escuela DO, Wydra K, Pintsuk J, Narvaez M, Corrales F, Zaniewska M, Agnati LF, Franco R, Tanganelli S, Ferraro L, Filip M, Fuxe K.

Neural Plast. 2016;2016:4827268. Epub 2016 Oct 30. Review.

27.

Detection of Missing Proteins Using the PRIDE Database as a Source of Mass Spectrometry Evidence.

Garin-Muga A, Odriozola L, Martínez-Val A, Del Toro N, Martínez R, Molina M, Cantero L, Rivera R, Garrido N, Dominguez F, Sanchez Del Pino MM, Vizcaíno JA, Corrales FJ, Segura V.

J Proteome Res. 2016 Nov 4;15(11):4101-4115. Epub 2016 Sep 15.

28.

Highlights of the Biology and Disease-driven Human Proteome Project, 2015-2016.

Van Eyk JE, Corrales FJ, Aebersold R, Cerciello F, Deutsch EW, Roncada P, Sanchez JC, Yamamoto T, Yang P, Zhang H, Omenn GS.

J Proteome Res. 2016 Nov 4;15(11):3979-3987. Epub 2016 Sep 20.

29.

Multicentric study of the effect of pre-analytical variables in the quality of plasma samples stored in biobanks using different complementary proteomic methods.

Mateos J, Carneiro I, Corrales F, Elortza F, Paradela A, Del Pino MS, Iloro I, Marcilla M, Mora MI, Valero L, Ciordia S, Fernández V, Fortuño MA, García-Sánchez I, Martínez R, Muñoz MA, Rodriguez C, Doménech N; ProteoRed Spanish Biobanks Network.

J Proteomics. 2017 Jan 6;150:109-120. doi: 10.1016/j.jprot.2016.09.003. Epub 2016 Sep 9.

PMID:
27620695
30.

Proteogenomic Analysis of Single Amino Acid Polymorphisms in Cancer Research.

Garin-Muga A, Corrales FJ, Segura V.

Adv Exp Med Biol. 2016;926:93-113.

PMID:
27686808
31.

Identification of a new potential mechanism responsible for severe bleeding in myeloma: immunoglobulins bind the heparin binding domain of antithrombin activating this endogenous anticoagulant.

Martínez-Martínez I, González-Porras JR, Cebeira MJ, de Arriba F, Espín S, Bohdan N, Corrales FJ, Corral J, Vicente V.

Haematologica. 2016 Oct;101(10):e423-e426. Epub 2016 Jun 16. No abstract available.

32.

SPARC gene deletion protects against toxic liver injury and is associated to an enhanced proliferative capacity and reduced oxidative stress response.

Peixoto E, Atorrasagasti C, Malvicini M, Fiore E, Rodriguez M, Garcia M, Finocchieto P, Poderoso JJ, Corrales F, Mazzolini G.

Oncotarget. 2016 May 18;10(41):4169-4179. doi: 10.18632/oncotarget.9456. eCollection 2019 Jun 25.

33.

Cardiac sympathetic denervation for intractable ventricular arrhythmias in Chagas disease.

Saenz LC, Corrales FM, Bautista W, Traina M, Meymandi S, Rodriguez DA, Tellez LJ, Vaseghi M, Garcia F, Shivkumar K, Bradfield JS.

Heart Rhythm. 2016 Jul;13(7):1388-94. doi: 10.1016/j.hrthm.2016.03.014. Epub 2016 Mar 9.

PMID:
26969783
34.

Glucose metabolism during fasting is altered in experimental porphobilinogen deaminase deficiency.

Collantes M, Serrano-Mendioroz I, Benito M, Molinet-Dronda F, Delgado M, Vinaixa M, Sampedro A, Enríquez de Salamanca R, Prieto E, Pozo MA, Peñuelas I, Corrales FJ, Barajas M, Fontanellas A.

Hum Mol Genet. 2016 Apr 1;25(7):1318-27. doi: 10.1093/hmg/ddw013. Epub 2016 Jan 21.

PMID:
26908609
35.

Methylthioadenosine (MTA) Regulates Liver Cells Proteome and Methylproteome: Implications in Liver Biology and Disease.

Bigaud E, Corrales FJ.

Mol Cell Proteomics. 2016 May;15(5):1498-510. doi: 10.1074/mcp.M115.055772. Epub 2016 Jan 27.

36.

Lamin A is involved in the development of vascular calcification induced by chronic kidney failure and phosphorus load.

Quirós-González I, Román-García P, Alonso-Montes C, Barrio-Vázquez S, Carrillo-López N, Naves-Díaz M, Mora MI, Corrales FJ, López-Hernández FJ, Ruiz-Torres MP, Cannata-Andía JB, Fernández-Martín JL.

Bone. 2016 Mar;84:160-168. doi: 10.1016/j.bone.2016.01.005. Epub 2016 Jan 6.

PMID:
26769003
37.

The proteome quest to understand biology and disease (HUPO 2014).

Gil C, Calvete JJ, Corrales FJ.

J Proteomics. 2015 Sep 8;127(Pt B):223-4. doi: 10.1016/j.jprot.2015.09.025. No abstract available.

PMID:
26481688
38.

Long-term metabolic correction of Wilson's disease in a murine model by gene therapy.

Murillo O, Luqui DM, Gazquez C, Martinez-Espartosa D, Navarro-Blasco I, Monreal JI, Guembe L, Moreno-Cermeño A, Corrales FJ, Prieto J, Hernandez-Alcoceba R, Gonzalez-Aseguinolaza G.

J Hepatol. 2016 Feb;64(2):419-426. doi: 10.1016/j.jhep.2015.09.014. Epub 2015 Sep 25.

PMID:
26409215
39.

Mudd's disease (MAT I/III deficiency): a survey of data for MAT1A homozygotes and compound heterozygotes.

Chien YH, Abdenur JE, Baronio F, Bannick AA, Corrales F, Couce M, Donner MG, Ficicioglu C, Freehauf C, Frithiof D, Gotway G, Hirabayashi K, Hofstede F, Hoganson G, Hwu WL, James P, Kim S, Korman SH, Lachmann R, Levy H, Lindner M, Lykopoulou L, Mayatepek E, Muntau A, Okano Y, Raymond K, Rubio-Gozalbo E, Scholl-Bürgi S, Schulze A, Singh R, Stabler S, Stuy M, Thomas J, Wagner C, Wilson WG, Wortmann S, Yamamoto S, Pao M, Blom HJ.

Orphanet J Rare Dis. 2015 Aug 20;10:99. doi: 10.1186/s13023-015-0321-y. Review.

40.

Discovery of nutritional biomarkers: future directions based on omics technologies.

Odriozola L, Corrales FJ.

Int J Food Sci Nutr. 2015 Jul;66 Suppl 1:S31-40. Review.

PMID:
26241009
41.

Integration of Proteomics and Transcriptomics Data Sets for the Analysis of a Lymphoma B-Cell Line in the Context of the Chromosome-Centric Human Proteome Project.

Díez P, Droste C, Dégano RM, González-Muñoz M, Ibarrola N, Pérez-Andrés M, Garin-Muga A, Segura V, Marko-Varga G, LaBaer J, Orfao A, Corrales FJ, De Las Rivas J, Fuentes M.

J Proteome Res. 2015 Sep 4;14(9):3530-40. doi: 10.1021/acs.jproteome.5b00474. Epub 2015 Aug 5.

PMID:
26216070
42.

In Vitro Transcription/Translation System: A Versatile Tool in the Search for Missing Proteins.

Horvatovich P, Végvári Á, Saul J, Park JG, Qiu J, Syring M, Pirrotte P, Petritis K, Tegeler TJ, Aziz M, Fuentes M, Diez P, Gonzalez-Gonzalez M, Ibarrola N, Droste C, De Las Rivas J, Gil C, Clemente F, Hernaez ML, Corrales FJ, Nilsson CL, Berven FS, Bischoff R, Fehniger TE, LaBaer J, Marko-Varga G.

J Proteome Res. 2015 Sep 4;14(9):3441-51. doi: 10.1021/acs.jproteome.5b00486. Epub 2015 Jul 28.

PMID:
26155874
43.

Proteogenomics Dashboard for the Human Proteome Project.

Tabas-Madrid D, Alves-Cruzeiro J, Segura V, Guruceaga E, Vialas V, Prieto G, García C, Corrales FJ, Albar JP, Pascual-Montano A.

J Proteome Res. 2015 Sep 4;14(9):3738-49. doi: 10.1021/acs.jproteome.5b00466. Epub 2015 Jul 16.

PMID:
26144527
44.

Quest for Missing Proteins: Update 2015 on Chromosome-Centric Human Proteome Project.

Horvatovich P, Lundberg EK, Chen YJ, Sung TY, He F, Nice EC, Goode RJ, Yu S, Ranganathan S, Baker MS, Domont GB, Velasquez E, Li D, Liu S, Wang Q, He QY, Menon R, Guan Y, Corrales FJ, Segura V, Casal JI, Pascual-Montano A, Albar JP, Fuentes M, Gonzalez-Gonzalez M, Diez P, Ibarrola N, Degano RM, Mohammed Y, Borchers CH, Urbani A, Soggiu A, Yamamoto T, Salekdeh GH, Archakov A, Ponomarenko E, Lisitsa A, Lichti CF, Mostovenko E, Kroes RA, Rezeli M, Végvári Á, Fehniger TE, Bischoff R, Vizcaíno JA, Deutsch EW, Lane L, Nilsson CL, Marko-Varga G, Omenn GS, Jeong SK, Lim JS, Paik YK, Hancock WS.

J Proteome Res. 2015 Sep 4;14(9):3415-31. doi: 10.1021/pr5013009. Epub 2015 Jul 23.

45.

Vitamin D-binding protein as a biomarker of active disease in acute intermittent porphyria.

Serrano-Mendioroz I, Sampedro A, Mora MI, Mauleón I, Segura V, Enríquez de Salamanca R, Harper P, Sardh E, Corrales FJ, Fontanellas A.

J Proteomics. 2015 Sep 8;127(Pt B):377-85. doi: 10.1016/j.jprot.2015.05.004. Epub 2015 May 13.

PMID:
25979770
46.

Corrigendum to "Clinical and metabolic findings in patients with methionine adenosyltransferase I/III deficiency detected by newborn screening" [Mol. Genet. Metab. 110 (2013) 218-221].

Couce ML, Dolores Bóveda M, García-Jimémez C, Balmaseda E, Vives I, Castiñeiras DE, Fernández-Marmiesse A, Fraga JM, Mudd SH, Corrales FJ.

Mol Genet Metab. 2015 Mar;114(3):486. doi: 10.1016/j.ymgme.2015.01.011. Epub 2015 Feb 11. No abstract available.

PMID:
25877476
47.

The Spanish biology/disease initiative within the human proteome project: Application to rheumatic diseases.

Ruiz-Romero C, Calamia V, Albar JP, Casal JI, Corrales FJ, Fernández-Puente P, Gil C, Mateos J, Vivanco F, Blanco FJ.

J Proteomics. 2015 Sep 8;127(Pt B):406-13. doi: 10.1016/j.jprot.2015.01.023. Epub 2015 Feb 14. Review.

PMID:
25686859
48.

Juan Pablo Albar (1953-2014).

Corrales F, Gil C, Paradela A.

Proteomics. 2015 Feb;15(4):625-6. doi: 10.1002/pmic.201570034. No abstract available.

PMID:
25676246
49.

Prediction of a missing protein expression map in the context of the human proteome project.

Guruceaga E, Sanchez del Pino MM, Corrales FJ, Segura V.

J Proteome Res. 2015 Mar 6;14(3):1350-60. doi: 10.1021/pr500850u. Epub 2015 Feb 5.

PMID:
25612097
50.

SPARC (secreted protein acidic and rich in cysteine) knockdown protects mice from acute liver injury by reducing vascular endothelial cell damage.

Peixoto E, Atorrasagasti C, Aquino JB, Militello R, Bayo J, Fiore E, Piccioni F, Salvatierra E, Alaniz L, García MG, Bataller R, Corrales F, Gidekel M, Podhajcer O, Colombo MI, Mazzolini G.

Gene Ther. 2015 Jan;22(1):9-19. doi: 10.1038/gt.2014.102. Epub 2014 Nov 20.

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