Format
Sort by
Items per page

Send to

Choose Destination

Search results

Items: 1 to 50 of 55

1.

Improved GaLV-TR Glycoproteins to Pseudotype Lentiviral Vectors: Impact of Viral Protease Activity in the Production of LV Pseudotypes.

Tomás HA, Mestre DA, Rodrigues AF, Guerreiro MR, Carrondo MJT, Coroadinha AS.

Mol Ther Methods Clin Dev. 2019 Aug 14;15:1-8. doi: 10.1016/j.omtm.2019.08.001. eCollection 2019 Dec 13.

2.

Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins.

Suárez-Patiño SF, Bernardino TC, Núñez EGF, Astray RM, Pereira CA, Soares HR, Coroadinha AS, Jorge SAC.

Cytotechnology. 2019 Oct;71(5):949-962. doi: 10.1007/s10616-019-00337-y. Epub 2019 Aug 17.

PMID:
31422494
3.

Enhancing Hepatitis C virus pseudoparticles infectivity through p7NS2 cellular expression.

Soares HR, Ferreira-Fernandes M, Almeida AI, Marchel M, Alves PM, Coroadinha AS.

J Virol Methods. 2019 Dec;274:113714. doi: 10.1016/j.jviromet.2019.113714. Epub 2019 Aug 11.

4.

Evaluation of AAV-mediated delivery of shRNA to target basal-like breast cancer genetic vulnerabilities.

Pinto C, Silva G, Ribeiro AS, Oliveira M, Garrido M, Bandeira VS, Nascimento A, Coroadinha AS, Peixoto C, Barbas A, Paredes J, Brito C, Alves PM.

J Biotechnol. 2019 Jul 20;300:70-77. doi: 10.1016/j.jbiotec.2019.05.016. Epub 2019 May 28.

PMID:
31150679
5.

Detection and Quantification of Label-Free Infectious Adenovirus Using a Switch-On Cell-Based Fluorescent Biosensor.

Guerreiro MR, Freitas DF, Alves PM, Coroadinha AS.

ACS Sens. 2019 Jun 28;4(6):1654-1661. doi: 10.1021/acssensors.9b00489. Epub 2019 Jun 5.

PMID:
31117363
6.

Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81.

Soares HR, Castro R, Tomás HA, Carrondo MJT, Alves PM, Coroadinha AS.

AMB Express. 2019 Feb 7;9(1):22. doi: 10.1186/s13568-019-0741-5.

7.

MicroRNA-146a controls functional plasticity in γδ T cells by targeting NOD1.

Schmolka N, Papotto PH, Romero PV, Amado T, Enguita FJ, Amorim A, Rodrigues AF, Gordon KE, Coroadinha AS, Boldin M, Serre K, Buck AH, Gomes AQ, Silva-Santos B.

Sci Immunol. 2018 May 4;3(23). pii: eaao1392. doi: 10.1126/sciimmunol.aao1392.

PMID:
29728425
8.

LentiPro26: novel stable cell lines for constitutive lentiviral vector production.

Tomás HA, Rodrigues AF, Carrondo MJT, Coroadinha AS.

Sci Rep. 2018 Mar 27;8(1):5271. doi: 10.1038/s41598-018-23593-y.

9.

Intracellular Delivery of HCV NS3p gene using vectored particles.

Lemos MAN, Patiño SFS, Bernardino TC, Coroadinha AS, Soares H, Astray RM, Pereira CA, Jorge SAC.

J Biotechnol. 2018 May 20;274:33-39. doi: 10.1016/j.jbiotec.2018.03.010. Epub 2018 Mar 22.

PMID:
29577966
10.

Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange.

Soares HR, Almeida AI, Tomás HA, Alves PM, Coroadinha AS.

Biotechnol Lett. 2018 Apr;40(4):633-639. doi: 10.1007/s10529-018-2515-6. Epub 2018 Jan 20.

11.

Disclosing the Parameters Leading to High Productivity of Retroviral Producer Cells Lines: Evaluating Random Versus Targeted Integration.

Bandeira VS, Tomás HA, Alici E, Carrondo MJ, Coroadinha AS.

Hum Gene Ther Methods. 2017 Apr;28(2):78-90. doi: 10.1089/hgtb.2016.149.

PMID:
28301970
12.

Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins.

Pina AS, Carvalho S, Dias AM, Guilherme M, Pereira AS, Caraça LT, Coroadinha AS, Lowe CR, Roque AC.

J Chromatogr A. 2016 Nov 11;1472:55-65. doi: 10.1016/j.chroma.2016.10.017. Epub 2016 Oct 19.

PMID:
27773392
13.

Retroviral particles are effectively purified on an affinity matrix containing peptides selected by phage-display.

Fernandes CS, Barbosa I, Castro R, Pina AS, Coroadinha AS, Barbas A, Roque AC.

Biotechnol J. 2016 Dec;11(12):1513-1524. doi: 10.1002/biot.201600025. Epub 2016 Sep 26.

PMID:
27491899
14.

Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production.

Carinhas N, Pais DA, Koshkin A, Fernandes P, Coroadinha AS, Carrondo MJ, Alves PM, Teixeira AP.

Sci Rep. 2016 Mar 23;6:23529. doi: 10.1038/srep23529.

15.

Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope.

Fernandes CS, Castro R, Coroadinha AS, Roque AC.

J Chromatogr A. 2016 Mar 18;1438:160-70. doi: 10.1016/j.chroma.2016.02.026. Epub 2016 Feb 10.

PMID:
26903473
16.

Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity.

Soares HR, Castro R, Tomás HA, Rodrigues AF, Gomes-Alves P, Bellier B, Klatzmann D, Carrondo MJT, Alves PM, Coroadinha AS.

Vaccine. 2016 Mar 18;34(13):1634-1641. doi: 10.1016/j.vaccine.2015.12.015. Epub 2016 Jan 12.

PMID:
26795367
17.

Palmitoylation Strengthens Cholesterol-dependent Multimerization and Fusion Activity of Human Cytomegalovirus Glycoprotein B (gB).

Patrone M, Coroadinha AS, Teixeira AP, Alves PM.

J Biol Chem. 2016 Feb 26;291(9):4711-22. doi: 10.1074/jbc.M115.682252. Epub 2015 Dec 22.

18.

Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity.

Alves M, Lamego J, Bandeiras T, Castro R, Tomás H, Coroadinha AS, Costa J, Simplício AL.

Biochem Biophys Rep. 2015 Nov 19;5:105-110. doi: 10.1016/j.bbrep.2015.11.018. eCollection 2016 Mar.

19.

Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology.

Rodrigues AF, Soares HR, Guerreiro MR, Alves PM, Coroadinha AS.

Biotechnol J. 2015 Sep;10(9):1329-44. doi: 10.1002/biot.201400387. Epub 2015 Jul 24. Review.

PMID:
26212697
20.

Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK.

Rodrigues AF, Guerreiro MR, Formas-Oliveira AS, Fernandes P, Blechert AK, Genzel Y, Alves PM, Hu WS, Coroadinha AS.

Biotechnol Bioeng. 2016 Jan;113(1):150-62. doi: 10.1002/bit.25691. Epub 2015 Sep 2.

PMID:
26134455
21.

Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells.

Castro R, Fernandes P, Laske T, Sousa MF, Genzel Y, Scharfenberg K, Alves PM, Coroadinha AS.

Appl Microbiol Biotechnol. 2015 Sep;99(17):7059-68. doi: 10.1007/s00253-015-6636-8. Epub 2015 May 21.

PMID:
25994255
22.

Robust Expansion of Human Pluripotent Stem Cells: Integration of Bioprocess Design With Transcriptomic and Metabolomic Characterization.

Silva MM, Rodrigues AF, Correia C, Sousa MF, Brito C, Coroadinha AS, Serra M, Alves PM.

Stem Cells Transl Med. 2015 Jul;4(7):731-42. doi: 10.5966/sctm.2014-0270. Epub 2015 May 15.

23.

Single-step cloning-screening method: a new tool for developing and studying high-titer viral vector producer cells.

Rodrigues AF, Formas-Oliveira AS, Guerreiro MR, Tomás HA, Alves PM, Coroadinha AS.

Gene Ther. 2015 Sep;22(9):685-95. doi: 10.1038/gt.2015.44. Epub 2015 May 4.

PMID:
25938191
24.

Canine helper-dependent vectors production: implications of Cre activity and co-infection on adenovirus propagation.

Fernandes P, Almeida AI, Kremer EJ, Alves PM, Coroadinha AS.

Sci Rep. 2015 Mar 16;5:9135. doi: 10.1038/srep09135.

25.

Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells.

Rodrigues AF, Carrondo MJ, Alves PM, Coroadinha AS.

Trends Biotechnol. 2014 Dec;32(12):602-7. doi: 10.1016/j.tibtech.2014.09.010. Epub 2014 Oct 28. Review.

PMID:
25450042
26.

Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors.

Fernandes P, Simão D, Guerreiro MR, Kremer EJ, Coroadinha AS, Alves PM.

Gene Ther. 2015 Jan;22(1):40-9. doi: 10.1038/gt.2014.92. Epub 2014 Oct 23.

PMID:
25338917
27.

Insect cell line development using FLP-mediated cassette exchange technology.

Vidigal J, Fernandes F, Coroadinha AS, Teixeira AP, Alves PM.

Methods Mol Biol. 2014;1104:15-27. doi: 10.1007/978-1-62703-733-4_2.

PMID:
24297406
28.

Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineering.

Rodrigues AF, Formas-Oliveira AS, Bandeira VS, Alves PM, Hu WS, Coroadinha AS.

Metab Eng. 2013 Nov;20:131-45. doi: 10.1016/j.ymben.2013.10.001. Epub 2013 Oct 11.

PMID:
24120735
29.

Impact of E1 and Cre on adenovirus vector amplification: developing MDCK CAV-2-E1 and E1-Cre transcomplementing cell lines.

Fernandes P, Santiago VM, Rodrigues AF, Tomás H, Kremer EJ, Alves PM, Coroadinha AS.

PLoS One. 2013;8(4):e60342. doi: 10.1371/journal.pone.0060342. Epub 2013 Apr 2.

30.

A methodology for detection and quantification of esterase activity.

Simplício AL, Coroadinha AS, Gilmer JF, Lamego J.

Methods Mol Biol. 2013;984:309-19. doi: 10.1007/978-1-62703-296-4_22.

PMID:
23386353
31.

Downstream processing of lentiviral vectors: releasing bottlenecks.

Bandeira V, Peixoto C, Rodrigues AF, Cruz PE, Alves PM, Coroadinha AS, Carrondo MJ.

Hum Gene Ther Methods. 2012 Aug;23(4):255-63. doi: 10.1089/hgtb.2012.059. Epub 2012 Aug 30.

PMID:
22934827
32.

Bioprocess development for canine adenovirus type 2 vectors.

Fernandes P, Peixoto C, Santiago VM, Kremer EJ, Coroadinha AS, Alves PM.

Gene Ther. 2013 Apr;20(4):353-60. doi: 10.1038/gt.2012.52. Epub 2012 Jul 5.

PMID:
22763405
33.

Flipase-mediated cassette exchange in Sf9 insect cells for stable gene expression.

Fernandes F, Vidigal J, Dias MM, Prather KL, Coroadinha AS, Teixeira AP, Alves PM.

Biotechnol Bioeng. 2012 Nov;109(11):2836-44. doi: 10.1002/bit.24542. Epub 2012 May 19.

PMID:
22565981
34.

Carboxylesterase 2 production and characterization in human cells: new insights into enzyme oligomerization and activity.

Lamego J, Cunha B, Peixoto C, Sousa MF, Alves PM, Simplício AL, Coroadinha AS.

Appl Microbiol Biotechnol. 2013 Feb;97(3):1161-73. doi: 10.1007/s00253-012-3994-3. Epub 2012 Mar 25.

PMID:
22446793
35.

Down-regulation of CD81 in human cells producing HCV-E1/E2 retroVLPs.

Rodrigues AF, Guerreiro MR, Castro R, Tomás HA, Dalba C, Klatzmann D, Alves PM, Carrondo MJ, Coroadinha AS.

BMC Proc. 2011 Nov 22;5 Suppl 8:P72. doi: 10.1186/1753-6561-5-S8-P72. eCollection 2011. No abstract available.

36.

Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production.

Rodrigues AF, Amaral AI, Veríssimo V, Alves PM, Coroadinha AS.

Biotechnol Bioeng. 2012 May;109(5):1269-79. doi: 10.1002/bit.24410. Epub 2012 Jan 2.

PMID:
22179842
37.

Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition.

Rodrigues AF, Guerreiro MR, Santiago VM, Dalba C, Klatzmann D, Alves PM, Carrondo MJ, Coroadinha AS.

Biotechnol Bioeng. 2011 Nov;108(11):2623-33. doi: 10.1002/bit.23231. Epub 2011 Jun 21.

PMID:
21656710
38.

Manufacturing of retroviruses.

Cruz PE, Rodrigues T, Carmo M, Wirth D, Amaral AI, Alves PM, Coroadinha AS.

Methods Mol Biol. 2011;737:157-82. doi: 10.1007/978-1-61779-095-9_7.

PMID:
21590397
39.

Detection and quantification of carboxylesterase 2 activity by capillary electrophoresis.

Lamego J, Coroadinha AS, Simplício AL.

Anal Chem. 2011 Feb 1;83(3):881-7. doi: 10.1021/ac102547c. Epub 2011 Jan 5.

PMID:
21207966
40.

Production of retroviral vectors: review.

Coroadinha AS, Gama-Norton L, Amaral AI, Hauser H, Alves PM, Cruz PE.

Curr Gene Ther. 2010 Dec;10(6):456-73. Review.

PMID:
21054246
41.

Integrated strategy for the production of therapeutic retroviral vectors.

Carrondo M, Panet A, Wirth D, Coroadinha AS, Cruz P, Falk H, Schucht R, Dupont F, Geny-Fiamma C, Merten OW, Hauser H.

Hum Gene Ther. 2011 Mar;22(3):370-9. doi: 10.1089/hum.2009.165. Epub 2011 Mar 2.

PMID:
21043806
42.

Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines.

Gama-Norton L, Herrmann S, Schucht R, Coroadinha AS, Löw R, Alves PM, Bartholomae CC, Schmidt M, Baum C, Schambach A, Hauser H, Wirth D.

Hum Gene Ther. 2010 Aug;21(8):979-91. doi: 10.1089/hum.2009.089.

PMID:
20222806
43.

Retroviral vector production under serum deprivation: The role of lipids.

Rodrigues AF, Carmo M, Alves PM, Coroadinha AS.

Biotechnol Bioeng. 2009 Dec 15;104(6):1171-81. doi: 10.1002/bit.22499.

PMID:
19655394
44.

Thermosensitivity of the reverse transcription process as an inactivation mechanism of lentiviral vectors.

Carmo M, Dias JD, Panet A, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE.

Hum Gene Ther. 2009 Oct;20(10):1168-76. doi: 10.1089/hum.2009.068.

PMID:
19537947
45.

Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer.

Carmo M, Alves A, Rodrigues AF, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE.

J Gene Med. 2009 Aug;11(8):670-8. doi: 10.1002/jgm.1353.

PMID:
19507176
46.

Improving retroviral vectors production: role of carbon sources in lipid biosynthesis.

Amaral AI, Coroadinha AS, Merten OW, Alves PM.

J Biotechnol. 2008 Nov 25;138(3-4):57-66. doi: 10.1016/j.jbiotec.2008.08.005. Epub 2008 Aug 27.

PMID:
18793684
47.

Impact of retroviral vector components stoichiometry on packaging cell lines: effects on productivity and vector quality.

Carrondo MJ, Merten OW, Haury M, Alves PM, Coroadinha AS.

Hum Gene Ther. 2008 Feb;19(2):199-210.

PMID:
18092921
48.

A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors.

Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D.

Mol Ther. 2006 Aug;14(2):285-92. Epub 2006 May 11.

49.

Relationship between retroviral vector membrane and vector stability.

Carmo M, Faria TQ, Falk H, Coroadinha AS, Teixeira M, Merten OW, Gény-Fiamma C, Alves PM, Danos O, Panet A, Carrondo MJ, Cruz PE.

J Gen Virol. 2006 May;87(Pt 5):1349-56.

PMID:
16603538
50.

Retrovirus producer cell line metabolism: implications on viral productivity.

Coroadinha AS, Alves PM, Santos SS, Cruz PE, Merten OW, Carrondo MJ.

Appl Microbiol Biotechnol. 2006 Oct;72(6):1125-35. Epub 2006 Apr 6.

PMID:
16598449

Supplemental Content

Loading ...
Support Center