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J Cell Sci. 2019 Sep 4;133(5). pii: jcs232322. doi: 10.1242/jcs.232322.

Distinct actin cytoskeleton behaviour in primary and immortalised T-cells.

Author information

1
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK.
2
Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA kumars04@mit.edu marco.fritzsche@rdm.ox.ac.uk.
3
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK kumars04@mit.edu marco.fritzsche@rdm.ox.ac.uk.
4
Kennedy Institute for Rheumatology, University of Oxford, Roosevelt Drive, Oxford OX3 7LF, UK.

Abstract

Cytoskeletal actin dynamics are crucial for the activation of T-cells. Immortalised Jurkat T-cells have been the model system of choice to examine and correlate the dynamics of the actin cytoskeleton and the immunological synapse leading to T-cell activation. However, it has remained unclear whether immortalised cellular systems, such as Jurkat T-cells can recapitulate the cytoskeletal behaviour of primary T-cells. Studies delineating the cytoskeletal behaviour of Jurkat T-cells in comparison to primary T-cells are lacking. Here, we employ live-cell super-resolution microscopy to investigate the cytoskeletal actin organisation and dynamics of living primary and immortalised Jurkat T-cells at the appropriate spatiotemporal resolution. Under comparable activation conditions, we found differences in the architectural organisation and dynamics of Jurkat and primary mouse and human T-cells. Although the three main actin network architectures in Jurkat T-cells were reminiscent of primary T-cells, there were differences in the organisation and molecular mechanisms underlying these networks. Our results highlight mechanistic distinctions in the T-cell model system most utilised to study cytoskeletal actin dynamics.

KEYWORDS:

Actin cytoskeleton; Immune synapse; Jurkat cells; Primary T-cells

PMID:
31413071
DOI:
10.1242/jcs.232322
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Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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