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A Role for FACT in RNA Polymerase II Promoter-Proximal Pausing.

Tettey TT, Gao X, Shao W, Li H, Story BA, Chitsazan AD, Glaser RL, Goode ZH, Seidel CW, Conaway RC, Zeitlinger J, Blanchette M, Conaway JW.

Cell Rep. 2019 Jun 25;27(13):3770-3779.e7. doi: 10.1016/j.celrep.2019.05.099.


Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events.

Noe Gonzalez M, Conaway JW, Conaway RC.

J Vis Exp. 2019 May 13;(147). doi: 10.3791/59497.


DNA-dependent protein kinase catalytic subunit (DNA-PKcs) contributes to incorporation of histone variant H2A.Z into nucleosomes.

Wang LY, He YX, Li M, Ding J, Sui Y, Conaway JW, Conaway RC, Wang F, Jin J, Cai Y.

Protein Cell. 2019 May 20. doi: 10.1007/s13238-019-0632-1. [Epub ahead of print] No abstract available.


Imaging-based assays for investigating functions of the RNA polymerase II elongation factor Elongin and the Elongin ubiquitin ligase.

Weems JC, Unruh JR, Slaughter BD, Conaway RC, Conaway JW.

Methods. 2019 Apr 15;159-160:157-164. doi: 10.1016/j.ymeth.2019.02.015. Epub 2019 Feb 19.


Frozen in Transcription: Cryo-EM Structures of Pol II Transcribing through a Nucleosome.

Noe Gonzalez M, Conaway RC, Conaway JW.

Mol Cell. 2018 Dec 6;72(5):802-804. doi: 10.1016/j.molcel.2018.11.027.


CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts.

Noe Gonzalez M, Sato S, Tomomori-Sato C, Conaway JW, Conaway RC.

Nat Commun. 2018 Aug 23;9(1):3392. doi: 10.1038/s41467-018-05923-w.


Schizosaccharomyces pombe Pol II transcription elongation factor ELL functions as part of a rudimentary super elongation complex.

Gopalan S, Gibbon DM, Banks CA, Zhang Y, Florens LA, Washburn MP, Dabas P, Sharma N, Seidel CW, Conaway RC, Conaway JW.

Nucleic Acids Res. 2018 Nov 2;46(19):10095-10105. doi: 10.1093/nar/gky713.


Metabolic Regulation of Transcription and Chromatin.

Conaway RC.

Annu Rev Biochem. 2018 Jun 20;87:23-25. doi: 10.1146/annurev-biochem-062917-012600.


O-Linked N-acetylglucosamine transferase 1 regulates global histone H4 acetylation via stabilization of the nonspecific lethal protein NSL3.

Wu D, Zhao L, Feng Z, Yu C, Ding J, Wang L, Wang F, Liu D, Zhu H, Xing F, Conaway JW, Conaway RC, Cai Y, Jin J.

J Biol Chem. 2017 Jun 16;292(24):10014-10025. doi: 10.1074/jbc.M117.781401. Epub 2017 Apr 27.


Cockayne syndrome B protein regulates recruitment of the Elongin A ubiquitin ligase to sites of DNA damage.

Weems JC, Slaughter BD, Unruh JR, Boeing S, Hall SM, McLaird MB, Yasukawa T, Aso T, Svejstrup JQ, Conaway JW, Conaway RC.

J Biol Chem. 2017 Apr 21;292(16):6431-6437. doi: 10.1074/jbc.C117.777946. Epub 2017 Mar 14.


Mediator structure and rearrangements required for holoenzyme formation.

Tsai KL, Yu X, Gopalan S, Chao TC, Zhang Y, Florens L, Washburn MP, Murakami K, Conaway RC, Conaway JW, Asturias FJ.

Nature. 2017 Apr 13;544(7649):196-201. doi: 10.1038/nature21393. Epub 2017 Mar 1.


Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme.

Sato S, Tomomori-Sato C, Tsai KL, Yu X, Sardiu M, Saraf A, Washburn MP, Florens L, Asturias FJ, Conaway RC, Conaway JW.

J Biol Chem. 2016 Dec 23;291(52):26886-26898. doi: 10.1074/jbc.M116.756098. Epub 2016 Nov 7.


Multiomic Analysis of the UV-Induced DNA Damage Response.

Boeing S, Williamson L, Encheva V, Gori I, Saunders RE, Instrell R, Aygün O, Rodriguez-Martinez M, Weems JC, Kelly GP, Conaway JW, Conaway RC, Stewart A, Howell M, Snijders AP, Svejstrup JQ.

Cell Rep. 2016 May 17;15(7):1597-1610. doi: 10.1016/j.celrep.2016.04.047. Epub 2016 May 12.


TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin.

Masuda Y, Takahashi H, Sato S, Tomomori-Sato C, Saraf A, Washburn MP, Florens L, Conaway RC, Conaway JW, Hatakeyama S.

Nat Commun. 2015 Jun 22;6:7299. doi: 10.1038/ncomms8299.


Assembly of the Elongin A Ubiquitin Ligase Is Regulated by Genotoxic and Other Stresses.

Weems JC, Slaughter BD, Unruh JR, Hall SM, McLaird MB, Gilmore JM, Washburn MP, Florens L, Yasukawa T, Aso T, Conaway JW, Conaway RC.

J Biol Chem. 2015 Jun 12;290(24):15030-41. doi: 10.1074/jbc.M114.632794. Epub 2015 Apr 15.


Orchestrating transcription with the Pol II CTD.

Conaway RC, Conaway JW.

Nat Rev Mol Cell Biol. 2015 Mar;16(3):128. doi: 10.1038/nrm3956. Epub 2015 Feb 18. No abstract available.


MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.

Takahashi H, Takigawa I, Watanabe M, Anwar D, Shibata M, Tomomori-Sato C, Sato S, Ranjan A, Seidel CW, Tsukiyama T, Mizushima W, Hayashi M, Ohkawa Y, Conaway JW, Conaway RC, Hatakeyama S.

Nat Commun. 2015 Jan 9;6:5941. doi: 10.1038/ncomms6941.


Regulation of the Rhp26ERCC6/CSB chromatin remodeler by a novel conserved leucine latch motif.

Wang L, Limbo O, Fei J, Chen L, Kim B, Luo J, Chong J, Conaway RC, Conaway JW, Ranish JA, Kadonaga JT, Russell P, Wang D.

Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18566-71. doi: 10.1073/pnas.1420227112. Epub 2014 Dec 15.


Conserved abundance and topological features in chromatin-remodeling protein interaction networks.

Sardiu ME, Gilmore JM, Groppe BD, Herman D, Ramisetty SR, Cai Y, Jin J, Conaway RC, Conaway JW, Florens L, Washburn MP.

EMBO Rep. 2015 Jan;16(1):116-26. doi: 10.15252/embr.201439403. Epub 2014 Nov 26.


Biochemical assays for analyzing activities of ATP-dependent chromatin remodeling enzymes.

Chen L, Ooi SK, Conaway JW, Conaway RC.

J Vis Exp. 2014 Oct 25;(92):e51721. doi: 10.3791/51721.


Generation and purification of human INO80 chromatin remodeling complexes and subcomplexes.

Chen L, Ooi SK, Conaway RC, Conaway JW.

J Vis Exp. 2014 Oct 23;(92):e51720. doi: 10.3791/51720.


Subunit Architecture and Functional Modular Rearrangements of the Transcriptional Mediator Complex.

Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ.

Cell. 2014 Jul 17;158(2):463. doi: 10.1016/j.cell.2014.06.036. Epub 2014 Jul 17. No abstract available.


Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.

Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ.

Cell. 2014 Jun 5;157(6):1430-44. doi: 10.1016/j.cell.2014.05.015. Epub 2014 May 29. Erratum in: Cell. 2014 Jul 17;158(2):463.


Multiple modes of regulation of the human Ino80 SNF2 ATPase by subunits of the INO80 chromatin-remodeling complex.

Chen L, Conaway RC, Conaway JW.

Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20497-502. doi: 10.1073/pnas.1317092110. Epub 2013 Dec 2.


Crosstalk between NSL histone acetyltransferase and MLL/SET complexes: NSL complex functions in promoting histone H3K4 di-methylation activity by MLL/SET complexes.

Zhao X, Su J, Wang F, Liu D, Ding J, Yang Y, Conaway JW, Conaway RC, Cao L, Wu D, Wu M, Cai Y, Jin J.

PLoS Genet. 2013 Nov;9(11):e1003940. doi: 10.1371/journal.pgen.1003940. Epub 2013 Nov 14.


Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6α.

Sela D, Conkright JJ, Chen L, Gilmore J, Washburn MP, Florens L, Conaway RC, Conaway JW.

J Biol Chem. 2013 Sep 6;288(36):26179-87. doi: 10.1074/jbc.M113.496968. Epub 2013 Jul 17.


Transcriptional properties of mammalian elongin A and its role in stress response.

Kawauchi J, Inoue M, Fukuda M, Uchida Y, Yasukawa T, Conaway RC, Conaway JW, Aso T, Kitajima S.

J Biol Chem. 2013 Aug 23;288(34):24302-15. doi: 10.1074/jbc.M113.496703. Epub 2013 Jul 3.


A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction.

Tsai KL, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Asturias FJ.

Nat Struct Mol Biol. 2013 May;20(5):611-9. doi: 10.1038/nsmb.2549. Epub 2013 Apr 7.


Immunoaffinity purification of protein complexes from Mammalian cells.

Tomomori-Sato C, Sato S, Conaway RC, Conaway JW.

Methods Mol Biol. 2013;977:273-87. doi: 10.1007/978-1-62703-284-1_22.


Activation of the SNF2 family ATPase ALC1 by poly(ADP-ribose) in a stable ALC1·PARP1·nucleosome intermediate.

Gottschalk AJ, Trivedi RD, Conaway JW, Conaway RC.

J Biol Chem. 2012 Dec 21;287(52):43527-32. doi: 10.1074/jbc.M112.401141. Epub 2012 Nov 6.


Transcriptional elongation factor elongin A regulates retinoic acid-induced gene expression during neuronal differentiation.

Yasukawa T, Bhatt S, Takeuchi T, Kawauchi J, Takahashi H, Tsutsui A, Muraoka T, Inoue M, Tsuda M, Kitajima S, Conaway RC, Conaway JW, Trainor PA, Aso T.

Cell Rep. 2012 Nov 29;2(5):1129-36. doi: 10.1016/j.celrep.2012.09.031. Epub 2012 Nov 1.


The Mediator complex and transcription elongation.

Conaway RC, Conaway JW.

Biochim Biophys Acta. 2013 Jan;1829(1):69-75. doi: 10.1016/j.bbagrm.2012.08.017. Epub 2012 Sep 13. Review.


The human EKC/KEOPS complex is recruited to Cullin2 ubiquitin ligases by the human tumour antigen PRAME.

Costessi A, Mahrour N, Sharma V, Stunnenberg R, Stoel MA, Tijchon E, Conaway JW, Conaway RC, Stunnenberg HG.

PLoS One. 2012;7(8):e42822. doi: 10.1371/journal.pone.0042822. Epub 2012 Aug 13.


Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.

Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC.

J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 10.1074/jbc.M112.369504. Epub 2012 May 10.


The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters.

Costessi A, Mahrour N, Tijchon E, Stunnenberg R, Stoel MA, Jansen PW, Sela D, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC, Stunnenberg HG.

EMBO J. 2011 Aug 5;30(18):3786-98. doi: 10.1038/emboj.2011.262.


Origins and activity of the Mediator complex.

Conaway RC, Conaway JW.

Semin Cell Dev Biol. 2011 Sep;22(7):729-34. doi: 10.1016/j.semcdb.2011.07.021. Epub 2011 Jul 28. Review.


Human mediator subunit MED26 functions as a docking site for transcription elongation factors.

Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE, Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW.

Cell. 2011 Jul 8;146(1):92-104. doi: 10.1016/j.cell.2011.06.005.


Function and regulation of the Mediator complex.

Conaway RC, Conaway JW.

Curr Opin Genet Dev. 2011 Apr;21(2):225-30. doi: 10.1016/j.gde.2011.01.013. Epub 2011 Feb 15. Review.


Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling.

Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway JW, Conaway RC.

J Biol Chem. 2011 Apr 1;286(13):11283-9. doi: 10.1074/jbc.M111.222505. Epub 2011 Feb 8.


O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1.

Capotosti F, Guernier S, Lammers F, Waridel P, Cai Y, Jin J, Conaway JW, Conaway RC, Herr W.

Cell. 2011 Feb 4;144(3):376-88. doi: 10.1016/j.cell.2010.12.030.


AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia.

Lin C, Smith ER, Takahashi H, Lai KC, Martin-Brown S, Florens L, Washburn MP, Conaway JW, Conaway RC, Shilatifard A.

Mol Cell. 2010 Feb 12;37(3):429-37. doi: 10.1016/j.molcel.2010.01.026.


Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex.

Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, Conaway JW, Conaway RC.

J Biol Chem. 2010 Feb 12;285(7):4268-72. doi: 10.1074/jbc.C109.087981. Epub 2009 Dec 14.


Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation.

Harreman M, Taschner M, Sigurdsson S, Anindya R, Reid J, Somesh B, Kong SE, Banks CA, Conaway RC, Conaway JW, Svejstrup JQ.

Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20705-10. doi: 10.1073/pnas.0907052106. Epub 2009 Nov 17.


Proteomics reveals a physical and functional link between hepatocyte nuclear factor 4alpha and transcription factor IID.

Takahashi H, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC.

J Biol Chem. 2009 Nov 20;284(47):32405-12. doi: 10.1074/jbc.M109.017954. Epub 2009 Oct 5.


Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler.

Gottschalk AJ, Timinszky G, Kong SE, Jin J, Cai Y, Swanson SK, Washburn MP, Florens L, Ladurner AG, Conaway JW, Conaway RC.

Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13770-4. doi: 10.1073/pnas.0906920106. Epub 2009 Aug 6.


Direct inhibition of RNA polymerase II transcription by RECQL5.

Aygün O, Xu X, Liu Y, Takahashi H, Kong SE, Conaway RC, Conaway JW, Svejstrup JQ.

J Biol Chem. 2009 Aug 28;284(35):23197-203. doi: 10.1074/jbc.M109.015750. Epub 2009 Jul 1.


Mediator comes out from the shadows.

Conaway RC, Conaway JW.

Structure. 2009 Apr 15;17(4):485-6. doi: 10.1016/j.str.2009.03.003. No abstract available.


The INO80 chromatin remodeling complex in transcription, replication and repair.

Conaway RC, Conaway JW.

Trends Biochem Sci. 2009 Feb;34(2):71-7. doi: 10.1016/j.tibs.2008.10.010. Epub 2008 Dec 6. Review.


MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.

Ding N, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Boyer TG.

J Biol Chem. 2009 Jan 30;284(5):2648-56. doi: 10.1074/jbc.M806514200. Epub 2008 Dec 2.


Mammalian Elongin A complex mediates DNA-damage-induced ubiquitylation and degradation of Rpb1.

Yasukawa T, Kamura T, Kitajima S, Conaway RC, Conaway JW, Aso T.

EMBO J. 2008 Dec 17;27(24):3256-66. doi: 10.1038/emboj.2008.249. Epub 2008 Nov 27.

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