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Antimicrob Agents Chemother. 2016 Jun 20;60(7):4394-7. doi: 10.1128/AAC.00444-16. Print 2016 Jul.

Genetic Features of MCR-1-Producing Colistin-Resistant Escherichia coli Isolates in South Africa.

Author information

1
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland INSERM European Unit (LEA) (INSERM, Paris, France), University of Fribourg, Fribourg, Switzerland laurent.poirel@unifr.ch.
2
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
3
Department of Clinical Microbiology, Ampath National Laboratory Services, Milpark Hospital, Johannesburg, South Africa.
4
Department of Clinical Microbiology and Molecular Biology, Ampath National Laboratory Services, National Referral Laboratory, Centurion, South Africa.
5
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland INSERM European Unit (LEA) (INSERM, Paris, France), University of Fribourg, Fribourg, Switzerland University of Lausanne and University Hospital Center, Lausanne, Switzerland.

Abstract

A series of colistin-resistant Escherichia coli clinical isolates was recovered from hospitalized and community patients in South Africa. Seven clonally unrelated isolates harbored the mcr-1 gene located on different plasmid backbones. Two distinct plasmids were fully sequenced, and identical 2,600-bp-long DNA sequences defining a mcr-1 cassette were identified. Promoter sequences responsible for the expression of mcr-1, deduced from the precise identification of the +1 transcription start site for mcr-1, were characterized.

PMID:
27161623
PMCID:
PMC4914673
DOI:
10.1128/AAC.00444-16
[Indexed for MEDLINE]
Free PMC Article

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