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Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes.

Chang C, Wu C, Osipiuk J, Siegel SD, Zhu S, Liu X, Joachimiak A, Clubb RT, Das A, Ton-That H.

Proc Natl Acad Sci U S A. 2019 Sep 3;116(36):18041-18049. doi: 10.1073/pnas.1907733116. Epub 2019 Aug 19.


NMR experiments redefine the hemoglobin binding properties of bacterial NEAr-iron Transporter domains.

Macdonald R, Mahoney BJ, Ellis-Guardiola K, Maresso A, Clubb RT.

Protein Sci. 2019 Aug;28(8):1513-1523. doi: 10.1002/pro.3662. Epub 2019 Jul 3.


Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function.

Tsui HS, Pham NVB, Amer BR, Bradley MC, Gosschalk JE, Gallagher-Jones M, Ibarra H, Clubb RT, Blaby-Haas CE, Clarke CF.

J Lipid Res. 2019 Jul;60(7):1293-1310. doi: 10.1194/jlr.M093534. Epub 2019 May 2.


Structure and mechanism of TagA, a novel membrane-associated glycosyltransferase that produces wall teichoic acids in pathogenic bacteria.

Kattke MD, Gosschalk JE, Martinez OE, Kumar G, Gale RT, Cascio D, Sawaya MR, Philips M, Brown ED, Clubb RT.

PLoS Pathog. 2019 Apr 19;15(4):e1007723. doi: 10.1371/journal.ppat.1007723. eCollection 2019 Apr.


Erratum for Siegel et al., "Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein".

Siegel SD, Amer BR, Wu C, Sawaya MR, Gosschalk JE, Clubb RT, Ton-That H.

MBio. 2019 Apr 16;10(2). pii: e00617-19. doi: 10.1128/mBio.00617-19. No abstract available.


Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.

Siegel SD, Amer BR, Wu C, Sawaya MR, Gosschalk JE, Clubb RT, Ton-That H.

MBio. 2019 Feb 19;10(1). pii: e01580-18. doi: 10.1128/mBio.01580-18. Erratum in: MBio. 2019 Apr 16;10(2):.


The Streptococcus pyogenes Shr protein captures human hemoglobin using two structurally unique binding domains.

Macdonald R, Cascio D, Collazo MJ, Phillips M, Clubb RT.

J Biol Chem. 2018 Nov 23;293(47):18365-18377. doi: 10.1074/jbc.RA118.005261. Epub 2018 Oct 9.


Protein Labeling via a Specific Lysine-Isopeptide Bond Using the Pilin Polymerizing Sortase from Corynebacterium diphtheriae.

McConnell SA, Amer BR, Muroski J, Fu J, Chang C, Ogorzalek Loo RR, Loo JA, Osipiuk J, Ton-That H, Clubb RT.

J Am Chem Soc. 2018 Jul 11;140(27):8420-8423. doi: 10.1021/jacs.8b05200. Epub 2018 Jun 28.


In vitro reconstitution of sortase-catalyzed pilus polymerization reveals structural elements involved in pilin cross-linking.

Chang C, Amer BR, Osipiuk J, McConnell SA, Huang IH, Hsieh V, Fu J, Nguyen HH, Muroski J, Flores E, Ogorzalek Loo RR, Loo JA, Putkey JA, Joachimiak A, Das A, Clubb RT, Ton-That H.

Proc Natl Acad Sci U S A. 2018 Jun 12;115(24):E5477-E5486. doi: 10.1073/pnas.1800954115. Epub 2018 May 29.


Stabilizing displayed proteins on vegetative Bacillus subtilis cells.

Huang GL, Gosschalk JE, Kim YS, Ogorzalek Loo RR, Clubb RT.

Appl Microbiol Biotechnol. 2018 Aug;102(15):6547-6565. doi: 10.1007/s00253-018-9062-x. Epub 2018 May 23.


Energetics underlying hemin extraction from human hemoglobin by Staphylococcus aureus.

Sjodt M, Macdonald R, Marshall JD, Clayton J, Olson JS, Phillips M, Gell DA, Wereszczynski J, Clubb RT.

J Biol Chem. 2018 May 4;293(18):6942-6957. doi: 10.1074/jbc.RA117.000803. Epub 2018 Mar 14.


Retraction for Anderson et al., "Assembly of Minicellulosomes on the Surface of Bacillus subtilis".

Anderson TD, Robson SA, Jiang XW, Malmirchegini GR, Fierobe HP, Lazazzera BA, Clubb RT.

Appl Environ Microbiol. 2018 Jan 31;84(4). pii: e02429-17. doi: 10.1128/AEM.02429-17. Print 2018 Feb 15. No abstract available.


Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.

Jacobitz AW, Kattke MD, Wereszczynski J, Clubb RT.

Adv Protein Chem Struct Biol. 2017;109:223-264. doi: 10.1016/bs.apcsb.2017.04.008. Epub 2017 Jun 5. Review.


Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies.

Sjodt M, Clubb RT.

Bio Protoc. 2017 Apr 5;7(7). pii: e2207. doi: 10.21769/BioProtoc.2207.


NMR structure-based optimization of Staphylococcus aureus sortase A pyridazinone inhibitors.

Chan AH, Yi SW, Weiner EM, Amer BR, Sue CK, Wereszczynski J, Dillen CA, Senese S, Torres JZ, McCammon JA, Miller LS, Jung ME, Clubb RT.

Chem Biol Drug Des. 2017 Sep;90(3):327-344. doi: 10.1111/cbdd.12962. Epub 2017 Mar 6.


Crystal Structure of the Streptomyces coelicolor Sortase E1 Transpeptidase Provides Insight into the Binding Mode of the Novel Class E Sorting Signal.

Kattke MD, Chan AH, Duong A, Sexton DL, Sawaya MR, Cascio D, Elliot MA, Clubb RT.

PLoS One. 2016 Dec 9;11(12):e0167763. doi: 10.1371/journal.pone.0167763. eCollection 2016.


The "Lid" in the Streptococcus pneumoniae SrtC1 Sortase Adopts a Rigid Structure that Regulates Substrate Access to the Active Site.

Jacobitz AW, Naziga EB, Yi SW, McConnell SA, Peterson R, Jung ME, Clubb RT, Wereszczynski J.

J Phys Chem B. 2016 Aug 25;120(33):8302-12. doi: 10.1021/acs.jpcb.6b01930. Epub 2016 May 5.


Rapid addition of unlabeled silent solubility tags to proteins using a new substrate-fused sortase reagent.

Amer BR, Macdonald R, Jacobitz AW, Liauw B, Clubb RT.

J Biomol NMR. 2016 Mar;64(3):197-205. doi: 10.1007/s10858-016-0019-z. Epub 2016 Feb 6.


Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication.

Tekeste SS, Wilkinson TA, Weiner EM, Xu X, Miller JT, Le Grice SF, Clubb RT, Chow SA.

J Virol. 2015 Dec;89(23):12058-69. doi: 10.1128/JVI.01471-15. Epub 2015 Sep 23.


Structure of the Bacillus anthracis Sortase A Enzyme Bound to Its Sorting Signal: A FLEXIBLE AMINO-TERMINAL APPENDAGE MODULATES SUBSTRATE ACCESS.

Chan AH, Yi SW, Terwilliger AL, Maresso AW, Jung ME, Clubb RT.

J Biol Chem. 2015 Oct 16;290(42):25461-74. doi: 10.1074/jbc.M115.670984. Epub 2015 Aug 31.


Identification of agents effective against multiple toxins and viruses by host-oriented cell targeting.

Zilbermintz L, Leonardi W, Jeong SY, Sjodt M, McComb R, Ho CL, Retterer C, Gharaibeh D, Zamani R, Soloveva V, Bavari S, Levitin A, West J, Bradley KA, Clubb RT, Cohen SN, Gupta V, Martchenko M.

Sci Rep. 2015 Aug 27;5:13476. doi: 10.1038/srep13476.


Native top-down mass spectrometry for the structural characterization of human hemoglobin.

Zhang J, Reza Malmirchegini G, Clubb RT, Loo JA.

Eur J Mass Spectrom (Chichester). 2015;21(3):221-31. doi: 10.1255/ejms.1340.


Solution structure of the PhoP DNA-binding domain from Mycobacterium tuberculosis.

Macdonald R, Sarkar D, Amer BR, Clubb RT.

J Biomol NMR. 2015 Sep;63(1):111-7. doi: 10.1007/s10858-015-9965-0. Epub 2015 Jul 25. No abstract available.


The structure of haemoglobin bound to the haemoglobin receptor IsdH from Staphylococcus aureus shows disruption of the native α-globin haem pocket.

Dickson CF, Jacques DA, Clubb RT, Guss JM, Gell DA.

Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1295-306. doi: 10.1107/S1399004715005817. Epub 2015 May 14.


The PRE-Derived NMR Model of the 38.8-kDa Tri-Domain IsdH Protein from Staphylococcus aureus Suggests That It Adaptively Recognizes Human Hemoglobin.

Sjodt M, Macdonald R, Spirig T, Chan AH, Dickson CF, Fabian M, Olson JS, Gell DA, Clubb RT.

J Mol Biol. 2016 Mar 27;428(6):1107-1129. doi: 10.1016/j.jmb.2015.02.008. Epub 2015 Feb 14.


Novel mechanism of hemin capture by Hbp2, the hemoglobin-binding hemophore from Listeria monocytogenes.

Malmirchegini GR, Sjodt M, Shnitkind S, Sawaya MR, Rosinski J, Newton SM, Klebba PE, Clubb RT.

J Biol Chem. 2014 Dec 12;289(50):34886-99. doi: 10.1074/jbc.M114.583013. Epub 2014 Oct 14.


A sweet new role for LCP enzymes in protein glycosylation.

Amer BR, Clubb RT.

Mol Microbiol. 2014 Dec;94(6):1197-200. doi: 10.1111/mmi.12825. Epub 2014 Nov 21.


Trichomonas vaginalis homolog of macrophage migration inhibitory factor induces prostate cell growth, invasiveness, and inflammatory responses.

Twu O, Dessí D, Vu A, Mercer F, Stevens GC, de Miguel N, Rappelli P, Cocco AR, Clubb RT, Fiori PL, Johnson PJ.

Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8179-84. doi: 10.1073/pnas.1321884111. Epub 2014 May 19.


Structural and computational studies of the Staphylococcus aureus sortase B-substrate complex reveal a substrate-stabilized oxyanion hole.

Jacobitz AW, Wereszczynski J, Yi SW, Amer BR, Huang GL, Nguyen AV, Sawaya MR, Jung ME, McCammon JA, Clubb RT.

J Biol Chem. 2014 Mar 28;289(13):8891-902. doi: 10.1074/jbc.M113.509273. Epub 2014 Feb 11.


Engineering microbial surfaces to degrade lignocellulosic biomass.

Huang GL, Anderson TD, Clubb RT.

Bioengineered. 2014 Mar-Apr;5(2):96-106. doi: 10.4161/bioe.27461. Epub 2013 Dec 18. Review.


Structure of the hemoglobin-IsdH complex reveals the molecular basis of iron capture by Staphylococcus aureus.

Dickson CF, Kumar KK, Jacques DA, Malmirchegini GR, Spirig T, Mackay JP, Clubb RT, Guss JM, Gell DA.

J Biol Chem. 2014 Mar 7;289(10):6728-38. doi: 10.1074/jbc.M113.545566. Epub 2014 Jan 14.


Axial ligand replacement mechanism in heme transfer from streptococcal heme-binding protein Shp to HtsA of the HtsABC transporter.

Ran Y, Malmirchegini GR, Clubb RT, Lei B.

Biochemistry. 2013 Sep 17;52(37):6537-47. doi: 10.1021/bi400965u. Epub 2013 Sep 5.


Discovery of Staphylococcus aureus sortase A inhibitors using virtual screening and the relaxed complex scheme.

Chan AH, Wereszczynski J, Amer BR, Yi SW, Jung ME, McCammon JA, Clubb RT.

Chem Biol Drug Des. 2013 Oct;82(4):418-28. doi: 10.1111/cbdd.12167.


Recombinant Bacillus subtilis that grows on untreated plant biomass.

Anderson TD, Miller JI, Fierobe HP, Clubb RT.

Appl Environ Microbiol. 2013 Feb;79(3):867-76. doi: 10.1128/AEM.02433-12. Epub 2012 Nov 26. Retraction in: Anderson TD, Miller JI, Fierobe HP, Clubb RT. Appl Environ Microbiol. 2015 Nov 15;81(22):7957.


Staphylococcus aureus uses a novel multidomain receptor to break apart human hemoglobin and steal its heme.

Spirig T, Malmirchegini GR, Zhang J, Robson SA, Sjodt M, Liu M, Krishna Kumar K, Dickson CF, Gell DA, Lei B, Loo JA, Clubb RT.

J Biol Chem. 2013 Jan 11;288(2):1065-78. doi: 10.1074/jbc.M112.419119. Epub 2012 Nov 6.


The binding mechanism, multiple binding modes, and allosteric regulation of Staphylococcus aureus Sortase A probed by molecular dynamics simulations.

Kappel K, Wereszczynski J, Clubb RT, McCammon JA.

Protein Sci. 2012 Dec;21(12):1858-71. doi: 10.1002/pro.2168.


Solution structure of the sortase required for efficient production of infectious Bacillus anthracis spores.

Robson SA, Jacobitz AW, Phillips ML, Clubb RT.

Biochemistry. 2012 Oct 9;51(40):7953-63. doi: 10.1021/bi300867t. Epub 2012 Sep 27.


Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies.

Sam MD, Clubb RT.

Methods Mol Biol. 2012;831:219-32. doi: 10.1007/978-1-61779-480-3_13.


Backbone 1H, 13C and 15N resonance assignments of the 39 kDa staphylococcal hemoglobin receptor IsdH.

Spirig T, Clubb RT.

Biomol NMR Assign. 2012 Oct;6(2):169-72. doi: 10.1007/s12104-011-9348-8. Epub 2011 Nov 19.


Sortase enzymes in Gram-positive bacteria.

Spirig T, Weiner EM, Clubb RT.

Mol Microbiol. 2011 Dec;82(5):1044-59. doi: 10.1111/j.1365-2958.2011.07887.x. Epub 2011 Nov 7. Review.


Structural basis for hemoglobin capture by Staphylococcus aureus cell-surface protein, IsdH.

Krishna Kumar K, Jacques DA, Pishchany G, Caradoc-Davies T, Spirig T, Malmirchegini GR, Langley DB, Dickson CF, Mackay JP, Clubb RT, Skaar EP, Guss JM, Gell DA.

J Biol Chem. 2011 Nov 4;286(44):38439-47. doi: 10.1074/jbc.M111.287300. Epub 2011 Sep 14.


Transient weak protein-protein complexes transfer heme across the cell wall of Staphylococcus aureus.

Villareal VA, Spirig T, Robson SA, Liu M, Lei B, Clubb RT.

J Am Chem Soc. 2011 Sep 14;133(36):14176-9. doi: 10.1021/ja203805b. Epub 2011 Aug 19.


Assembly of minicellulosomes on the surface of Bacillus subtilis.

Anderson TD, Robson SA, Jiang XW, Malmirchegini GR, Fierobe HP, Lazazzera BA, Clubb RT.

Appl Environ Microbiol. 2011 Jul;77(14):4849-58. doi: 10.1128/AEM.02599-10. Epub 2011 May 27. Retraction in: Appl Environ Microbiol. 2018 Jan 31;84(4):.


Site-directed spin labeling electron paramagnetic resonance study of the ORF1 protein from a mouse L1 retrotransposon.

Januszyk K, Fleissner MR, Atchabahian L, Shieh FK, Altenbach C, Martin SL, Guo F, Hubbell WL, Clubb RT.

Protein Sci. 2011 Jul;20(7):1231-43. doi: 10.1002/pro.651. Epub 2011 Jun 14.


Anthrax toxin receptor 1/tumor endothelial marker 8: mutation of conserved inserted domain residues overrides cytosolic control of protective antigen binding.

Ramey JD, Villareal VA, Ng C, Ward SC, Xiong JP, Clubb RT, Bradley KA.

Biochemistry. 2010 Aug 31;49(34):7403-10. doi: 10.1021/bi100887w.


A heteronuclear zero quantum coherence Nz-exchange experiment that resolves resonance overlap and its application to measure the rates of heme binding to the IsdC protein.

Robson SA, Peterson R, Bouchard LS, Villareal VA, Clubb RT.

J Am Chem Soc. 2010 Jul 21;132(28):9522-3. doi: 10.1021/ja1017865.


Structure of the dimerization domain of DiGeorge critical region 8.

Senturia R, Faller M, Yin S, Loo JA, Cascio D, Sawaya MR, Hwang D, Clubb RT, Guo F.

Protein Sci. 2010 Jul;19(7):1354-65. doi: 10.1002/pro.414.


The Sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusual active site architecture.

Weiner EM, Robson S, Marohn M, Clubb RT.

J Biol Chem. 2010 Jul 23;285(30):23433-43. doi: 10.1074/jbc.M110.135434. Epub 2010 May 19.


Discovery and structure-activity relationship analysis of Staphylococcus aureus sortase A inhibitors.

Suree N, Yi SW, Thieu W, Marohn M, Damoiseaux R, Chan A, Jung ME, Clubb RT.

Bioorg Med Chem. 2009 Oct 15;17(20):7174-85. doi: 10.1016/j.bmc.2009.08.067. Epub 2009 Sep 6.


The structure of the Staphylococcus aureus sortase-substrate complex reveals how the universally conserved LPXTG sorting signal is recognized.

Suree N, Liew CK, Villareal VA, Thieu W, Fadeev EA, Clemens JJ, Jung ME, Clubb RT.

J Biol Chem. 2009 Sep 4;284(36):24465-77. doi: 10.1074/jbc.M109.022624. Epub 2009 Jul 10.

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