Send to

Choose Destination
J Cell Biol. 2020 Mar 2;219(3). pii: e201902127. doi: 10.1083/jcb.201902127.

TORC1 regulates vacuole membrane composition through ubiquitin- and ESCRT-dependent microautophagy.

Author information

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI.
Life Sciences Institute, University of Michigan, Ann Arbor, MI.
Institute for Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.


Cellular adaptation in response to nutrient limitation requires the induction of autophagy and lysosome biogenesis for the efficient recycling of macromolecules. Here, we discovered that starvation and TORC1 inactivation not only lead to the up-regulation of autophagy and vacuole proteins involved in recycling but also result in the down-regulation of many vacuole membrane proteins to supply amino acids as part of a vacuole remodeling process. Down-regulation of vacuole membrane proteins is initiated by ubiquitination, which is accomplished by the coordination of multiple E3 ubiquitin ligases, including Rsp5, the Dsc complex, and a newly characterized E3 ligase, Pib1. The Dsc complex is negatively regulated by TORC1 through the Rim15-Ume6 signaling cascade. After ubiquitination, vacuole membrane proteins are sorted into the lumen for degradation by ESCRT-dependent microautophagy. Thus, our study uncovered a complex relationship between TORC1 inactivation and vacuole biogenesis.


Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center