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Items: 26


Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures.

Chokhawala HA, Roche CM, Kim TW, Atreya ME, Vegesna N, Dana CM, Blanch HW, Clark DS.

BMC Biotechnol. 2015 Feb 21;15:11. doi: 10.1186/s12896-015-0118-z.


The importance of pyroglutamate in cellulase Cel7A.

Dana CM, Dotson-Fagerstrom A, Roche CM, Kal SM, Chokhawala HA, Blanch HW, Clark DS.

Biotechnol Bioeng. 2014 Apr;111(4):842-7. doi: 10.1002/bit.25178. Epub 2014 Jan 28.


An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support.

Fei Y, Landry JP, Li Y, Yu H, Lau K, Huang S, Chokhawala HA, Chen X, Zhu XD.

Rev Sci Instrum. 2013 Nov;84(11):114102. doi: 10.1063/1.4826352.


N-Terminal labeling of filamentous phage to create cancer marker imaging agents.

Carrico ZM, Farkas ME, Zhou Y, Hsiao SC, Marks JD, Chokhawala H, Clark DS, Francis MB.

ACS Nano. 2012 Aug 28;6(8):6675-80. doi: 10.1021/nn301134z. Epub 2012 Aug 6.


Cross-comparison of protein recognition of sialic acid diversity on two novel sialoglycan microarrays.

Padler-Karavani V, Song X, Yu H, Hurtado-Ziola N, Huang S, Muthana S, Chokhawala HA, Cheng J, Verhagen A, Langereis MA, Kleene R, Schachner M, de Groot RJ, Lasanajak Y, Matsuda H, Schwab R, Chen X, Smith DF, Cummings RD, Varki A.

J Biol Chem. 2012 Jun 29;287(27):22593-608. doi: 10.1074/jbc.M112.359323. Epub 2012 May 1.


Fluorescent labeling agents change binding profiles of glycan-binding proteins.

Fei Y, Sun YS, Li Y, Lau K, Yu H, Chokhawala HA, Huang S, Landry JP, Chen X, Zhu X.

Mol Biosyst. 2011 Dec;7(12):3343-52. doi: 10.1039/c1mb05332a. Epub 2011 Oct 18.


High-throughput in vitro glycoside hydrolase (HIGH) screening for enzyme discovery.

Kim TW, Chokhawala HA, Hess M, Dana CM, Baer Z, Sczyrba A, Rubin EM, Blanch HW, Clark DS.

Angew Chem Int Ed Engl. 2011 Nov 18;50(47):11215-8. doi: 10.1002/anie.201104685. Epub 2011 Sep 16. No abstract available.


A sialylated glycan microarray reveals novel interactions of modified sialic acids with proteins and viruses.

Song X, Yu H, Chen X, Lasanajak Y, Tappert MM, Air GM, Tiwari VK, Cao H, Chokhawala HA, Zheng H, Cummings RD, Smith DF.

J Biol Chem. 2011 Sep 9;286(36):31610-22. doi: 10.1074/jbc.M111.274217. Epub 2011 Jul 12.


Identification and characterization of a multidomain hyperthermophilic cellulase from an archaeal enrichment.

Graham JE, Clark ME, Nadler DC, Huffer S, Chokhawala HA, Rowland SE, Blanch HW, Clark DS, Robb FT.

Nat Commun. 2011 Jul 5;2:375. doi: 10.1038/ncomms1373.


Human xeno-autoantibodies against a non-human sialic acid serve as novel serum biomarkers and immunotherapeutics in cancer.

Padler-Karavani V, Hurtado-Ziola N, Pu M, Yu H, Huang S, Muthana S, Chokhawala HA, Cao H, Secrest P, Friedmann-Morvinski D, Singer O, Ghaderi D, Verma IM, Liu YT, Messer K, Chen X, Varki A, Schwab R.

Cancer Res. 2011 May 1;71(9):3352-63. doi: 10.1158/0008-5472.CAN-10-4102. Epub 2011 Apr 19.


Metagenomic discovery of biomass-degrading genes and genomes from cow rumen.

Hess M, Sczyrba A, Egan R, Kim TW, Chokhawala H, Schroth G, Luo S, Clark DS, Chen F, Zhang T, Mackie RI, Pennacchio LA, Tringe SG, Visel A, Woyke T, Wang Z, Rubin EM.

Science. 2011 Jan 28;331(6016):463-7. doi: 10.1126/science.1200387.


Binding modules alter the activity of chimeric cellulases: Effects of biomass pretreatment and enzyme source.

Kim TW, Chokhawala HA, Nadler DC, Blanch HW, Clark DS.

Biotechnol Bioeng. 2010 Nov 1;107(4):601-11. doi: 10.1002/bit.22856. Erratum in: Biotechnol Bioeng. 2011 Sep;108(9):2247. Nadler, Dana [corrected to Nadler, Dana C].


Sialidase substrate specificity studies using chemoenzymatically synthesized sialosides containing C5-modified sialic acids.

Cao H, Li Y, Lau K, Muthana S, Yu H, Cheng J, Chokhawala HA, Sugiarto G, Zhang L, Chen X.

Org Biomol Chem. 2009 Dec 21;7(24):5137-45. doi: 10.1039/b916305k. Epub 2009 Oct 26.


Combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides.

Chokhawala HA, Huang S, Lau K, Yu H, Cheng J, Thon V, Hurtado-Ziola N, Guerrero JA, Varki A, Chen X.

ACS Chem Biol. 2008 Sep 19;3(9):567-76. doi: 10.1021/cb800127n. Epub 2008 Aug 27.


Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: potential implications for disease.

Padler-Karavani V, Yu H, Cao H, Chokhawala H, Karp F, Varki N, Chen X, Varki A.

Glycobiology. 2008 Oct;18(10):818-30. doi: 10.1093/glycob/cwn072. Epub 2008 Jul 31.


Multifunctionality of Campylobacter jejuni sialyltransferase CstII: characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities.

Cheng J, Yu H, Lau K, Huang S, Chokhawala HA, Li Y, Tiwari VK, Chen X.

Glycobiology. 2008 Sep;18(9):686-97. doi: 10.1093/glycob/cwn047. Epub 2008 May 28.


N-Terminal 112 amino acid residues are not required for the sialyltransferase activity of Photobacterium damsela alpha2,6-sialyltransferase.

Sun M, Li Y, Chokhawala HA, Henning R, Chen X.

Biotechnol Lett. 2008 Apr;30(4):671-6. Epub 2007 Nov 8.


Enzymatic synthesis of fluorinated mechanistic probes for sialidases and sialyltransferases.

Chokhawala HA, Cao H, Yu H, Chen X.

J Am Chem Soc. 2007 Sep 5;129(35):10630-1. Epub 2007 Aug 14. No abstract available.


The Hd0053 gene of Haemophilus ducreyi encodes an alpha2,3-sialyltransferase.

Li Y, Sun M, Huang S, Yu H, Chokhawala HA, Thon V, Chen X.

Biochem Biophys Res Commun. 2007 Sep 21;361(2):555-60. Epub 2007 Jul 23.


Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids.

Yu H, Chokhawala HA, Varki A, Chen X.

Org Biomol Chem. 2007 Aug 7;5(15):2458-63. Epub 2007 Jun 27.


Crystal structures of Pasteurella multocida sialyltransferase complexes with acceptor and donor analogues reveal substrate binding sites and catalytic mechanism.

Ni L, Chokhawala HA, Cao H, Henning R, Ng L, Huang S, Yu H, Chen X, Fisher AJ.

Biochemistry. 2007 May 29;46(21):6288-98. Epub 2007 May 8.


Cytidine 5'-monophosphate (CMP)-induced structural changes in a multifunctional sialyltransferase from Pasteurella multocida.

Ni L, Sun M, Yu H, Chokhawala H, Chen X, Fisher AJ.

Biochemistry. 2006 Feb 21;45(7):2139-48.


A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries.

Yu H, Chokhawala H, Karpel R, Yu H, Wu B, Zhang J, Zhang Y, Jia Q, Chen X.

J Am Chem Soc. 2005 Dec 21;127(50):17618-9.


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