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Items: 1 to 50 of 86

1.

CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci.

Mao S, Ying Y, Wu X, Krueger CJ, Chen AK.

Nucleic Acids Res. 2019 Aug 31. pii: gkz752. doi: 10.1093/nar/gkz752. [Epub ahead of print]

PMID:
31504824
2.

Live-Cell Imaging of Long Noncoding RNAs Using Molecular Beacons.

Ying Y, Mao S, Krueger CJ, Chen AK.

Methods Mol Biol. 2019;2038:21-33. doi: 10.1007/978-1-4939-9674-2_2.

PMID:
31407275
3.

The impact of obesity on severity of solid organ injury in the adult population at a Level I trauma center.

Chen AK, Jeffcoach D, Stivers JC, McCullough KA, Dirks RC, Boehnke RJ, Sue L, Kwok AM, Wolfe MM, Davis JW.

Trauma Surg Acute Care Open. 2019 Jul 12;4(1):e000318. doi: 10.1136/tsaco-2019-000318. eCollection 2019.

4.

Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms.

Wu X, Mao S, Ying Y, Krueger CJ, Chen AK.

Genomics Proteomics Bioinformatics. 2019 Apr;17(2):119-128. doi: 10.1016/j.gpb.2018.10.001. Epub 2019 Jan 30. Review.

5.

Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular Beacons.

Chen M, Mao S, Wu X, Ma Z, Yang Y, Krueger CJ, Chen AK.

Methods Mol Biol. 2019;1870:23-39. doi: 10.1007/978-1-4939-8808-2_2.

PMID:
30539544
6.

Roles of Gag-RNA interactions in HIV-1 virus assembly deciphered by single-molecule localization microscopy.

Yang Y, Qu N, Tan J, Rushdi MN, Krueger CJ, Chen AK.

Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):6721-6726. doi: 10.1073/pnas.1805728115. Epub 2018 Jun 11.

7.

A CRISPR/molecular beacon hybrid system for live-cell genomic imaging.

Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK.

Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304.

8.

Improved erythroid differentiation of multiple human pluripotent stem cell lines in microcarrier culture by modulation of Wnt/β-Catenin signaling.

Sivalingam J, Chen HY, Yang BX, Lim ZR, Lam ATL, Woo TL, Chen AK, Reuveny S, Loh YH, Oh SK.

Haematologica. 2018 Jul;103(7):e279-e283. doi: 10.3324/haematol.2017.180919. Epub 2018 Mar 8. No abstract available.

9.

Defined Serum-Free Medium for Bioreactor Culture of an Immortalized Human Erythroblast Cell Line.

Lee E, Lim ZR, Chen HY, Yang BX, Lam AT, Chen AK, Sivalingam J, Reuveny S, Loh YH, Oh SK.

Biotechnol J. 2018 Apr;13(4):e1700567. doi: 10.1002/biot.201700567. Epub 2018 Feb 6.

PMID:
29330927
10.

Optimizing Molecular Beacons for Intracellular Analysis of RNA.

Chen M, Yang Y, Krueger CJ, Chen AK.

Methods Mol Biol. 2018;1649:243-257. doi: 10.1007/978-1-4939-7213-5_16.

PMID:
29130202
11.

Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons.

Yang Y, Chen M, Krueger CJ, Tsourkas A, Chen AK.

Methods Mol Biol. 2018;1649:231-242. doi: 10.1007/978-1-4939-7213-5_15.

PMID:
29130201
12.

Immature HIV-1 lattice assembly dynamics are regulated by scaffolding from nucleic acid and the plasma membrane.

Pak AJ, Grime JMA, Sengupta P, Chen AK, Durumeric AEP, Srivastava A, Yeager M, Briggs JAG, Lippincott-Schwartz J, Voth GA.

Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):E10056-E10065. doi: 10.1073/pnas.1706600114. Epub 2017 Nov 7.

13.

Discussion of: "Day versus night laparoscopic cholecystectomy for acute cholecystitis: A comparison of outcomes and cost".

Siada SS, Schaetzel SS, Chen AK, Hoang HD, Wilder FG, Dirks RC, Kaups KL, Davis JW.

Am J Surg. 2017 Dec;214(6):1028-1029. doi: 10.1016/j.amjsurg.2017.09.028. Epub 2017 Sep 30. No abstract available.

PMID:
28987412
14.

Engineering Novel Molecular Beacon Constructs to Study Intracellular RNA Dynamics and Localization.

Ma Z, Wu X, Krueger CJ, Chen AK.

Genomics Proteomics Bioinformatics. 2017 Oct;15(5):279-286. doi: 10.1016/j.gpb.2017.04.004. Epub 2017 Sep 21. Review.

15.

Day versus night laparoscopic cholecystectomy for acute cholecystitis: A comparison of outcomes and cost.

Siada SS, Schaetzel SS, Chen AK, Hoang HD, Wilder FG, Dirks RC, Kaups KL, Davis JW.

Am J Surg. 2017 Dec;214(6):1024-1027. doi: 10.1016/j.amjsurg.2017.08.027. Epub 2017 Sep 18.

PMID:
28941725
16.

Immobilization of vitronectin-binding heparan sulfates onto surfaces to support human pluripotent stem cells.

Yap L, Murali S, Bhakta G, Titmarsh DM, Chen AK, Chiin Sim L, Bardor M, Lim YM, Goh JCH, Oh SKW, Choo ABH, van Wijnen AJ, Robinson DE, Whittle JD, Birch WR, Short RD, Nurcombe V, Cool SM.

J Biomed Mater Res B Appl Biomater. 2018 Jul;106(5):1887-1896. doi: 10.1002/jbm.b.33999. Epub 2017 Sep 20.

PMID:
28941021
17.

Inhibition of retroviral Gag assembly by non-silencing miRNAs promotes autophagic viral degradation.

Qu N, Ma Z, Zhang M, Rushdi MN, Krueger CJ, Chen AK.

Protein Cell. 2018 Jul;9(7):640-651. doi: 10.1007/s13238-017-0461-z. Epub 2017 Sep 7.

18.

Immature Midbrain Dopaminergic Neurons Derived from Floor-Plate Method Improve Cell Transplantation Therapy Efficacy for Parkinson's Disease.

Qiu L, Liao MC, Chen AK, Wei S, Xie S, Reuveny S, Zhou ZD, Hunziker W, Tan EK, Oh SKW, Zeng L.

Stem Cells Transl Med. 2017 Sep;6(9):1803-1814. doi: 10.1002/sctm.16-0470. Epub 2017 Jun 26.

19.

A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.

Chen M, Ma Z, Wu X, Mao S, Yang Y, Tan J, Krueger CJ, Chen AK.

Sci Rep. 2017 May 8;7(1):1550. doi: 10.1038/s41598-017-01740-1.

20.

Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD.

Yu X, Zhang Y, Li T, Ma Z, Jia H, Chen Q, Zhao Y, Zhai L, Zhong R, Li C, Zou X, Meng J, Chen AK, Puri PL, Chen M, Zhu D.

Nat Commun. 2017 Jan 16;8:14016. doi: 10.1038/ncomms14016.

21.

Biodegradable poly-ε-caprolactone microcarriers for efficient production of human mesenchymal stromal cells and secreted cytokines in batch and fed-batch bioreactors.

Lam AT, Li J, Toh JP, Sim EJ, Chen AK, Chan JK, Choolani M, Reuveny S, Birch WR, Oh SK.

Cytotherapy. 2017 Mar;19(3):419-432. doi: 10.1016/j.jcyt.2016.11.009. Epub 2016 Dec 22.

PMID:
28017598
22.

Interprofessional education in the clinical setting: A qualitative look at the preceptor's perspective in training advanced practice nursing students.

Chen AK, Rivera J, Rotter N, Green E, Kools S.

Nurse Educ Pract. 2016 Nov;21:29-36. doi: 10.1016/j.nepr.2016.09.006. Epub 2016 Sep 28.

23.

Superior Red Blood Cell Generation from Human Pluripotent Stem Cells Through a Novel Microcarrier-Based Embryoid Body Platform.

Sivalingam J, Lam AT, Chen HY, Yang BX, Chen AK, Reuveny S, Loh YH, Oh SK.

Tissue Eng Part C Methods. 2016 Aug;22(8):765-80. doi: 10.1089/ten.TEC.2015.0579. Epub 2016 Aug 1.

PMID:
27392822
24.

The effect of Mind Body Medicine course on medical student empathy: a pilot study.

Chen AK, Kumar A, Haramati A.

Med Educ Online. 2016 Jun 27;21:31196. doi: 10.3402/meo.v21.31196. eCollection 2016.

25.

Single-molecule detection and tracking of RNA transcripts in living cells using phosphorothioate-optimized 2'-O-methyl RNA molecular beacons.

Zhao D, Yang Y, Qu N, Chen M, Ma Z, Krueger CJ, Behlke MA, Chen AK.

Biomaterials. 2016 Sep;100:172-83. doi: 10.1016/j.biomaterials.2016.05.022. Epub 2016 May 24.

26.

Microcarrier-Expanded Neural Progenitor Cells Can Survive, Differentiate, and Innervate Host Neurons Better When Transplanted as Aggregates.

Qiu L, Lim YM, Chen AK, Reuveny S, Oh SK, Tan EK, Zeng L.

Cell Transplant. 2016;25(7):1343-57. doi: 10.3727/096368915X690378. Epub 2015 Dec 30.

PMID:
26720780
27.

Integrated processes for expansion and differentiation of human pluripotent stem cells in suspended microcarriers cultures.

Lam AT, Chen AK, Ting SQ, Reuveny S, Oh SK.

Biochem Biophys Res Commun. 2016 May 6;473(3):764-8. doi: 10.1016/j.bbrc.2015.09.079. Epub 2015 Sep 15. Review.

PMID:
26385176
28.

Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures.

Lam AT, Li J, Chen AK, Birch WR, Reuveny S, Oh SK.

Biores Open Access. 2015 Apr 1;4(1):242-57. doi: 10.1089/biores.2015.0010. eCollection 2015.

29.

Serum-free media formulations are cell line-specific and require optimization for microcarrier culture.

Tan KY, Teo KL, Lim JF, Chen AK, Choolani M, Reuveny S, Chan J, Oh SK.

Cytotherapy. 2015 Aug;17(8):1152-65. doi: 10.1016/j.jcyt.2015.05.001. Erratum in: Cytotherapy. 2015 Dec;17(12):1845. Choolani, Mahesh [added]; Chan, Jerry [added].

PMID:
26139547
30.

Increasing efficiency of human mesenchymal stromal cell culture by optimization of microcarrier concentration and design of medium feed.

Chen AK, Chew YK, Tan HY, Reuveny S, Weng Oh SK.

Cytotherapy. 2015 Feb;17(2):163-73. doi: 10.1016/j.jcyt.2014.08.011. Epub 2014 Oct 7.

PMID:
25304664
31.

Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture.

Lam AT, Chen AK, Li J, Birch WR, Reuveny S, Oh SK.

Stem Cell Res Ther. 2014 Sep 15;5(5):110. doi: 10.1186/scrt498.

32.

MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production.

Chen AK, Sengupta P, Waki K, Van Engelenburg SB, Ochiya T, Ablan SD, Freed EO, Lippincott-Schwartz J.

Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2676-83. doi: 10.1073/pnas.1408037111. Epub 2014 Jun 17.

33.

Cationic surface charge combined with either vitronectin or laminin dictates the evolution of human embryonic stem cells/microcarrier aggregates and cell growth in agitated cultures.

Lam AT, Li J, Chen AK, Reuveny S, Oh SK, Birch WR.

Stem Cells Dev. 2014 Jul 15;23(14):1688-703. doi: 10.1089/scd.2013.0645. Epub 2014 Apr 25.

34.

In prehypertension leukocytosis is associated with body mass index but not with blood pressure or incident hypertension.

Julius S, Egan BM, Kaciroti NA, Nesbitt SD, Chen AK; TROPHY investigators.

J Hypertens. 2014 Feb;32(2):251-9. doi: 10.1097/HJH.0000000000000032.

PMID:
24275841
35.

Inhibition of ROCK-myosin II signaling pathway enables culturing of human pluripotent stem cells on microcarriers without extracellular matrix coating.

Chen AK, Chen X, Lim YM, Reuveny S, Oh SK.

Tissue Eng Part C Methods. 2014 Mar;20(3):227-38. doi: 10.1089/ten.TEC.2013.0191. Epub 2013 Jul 25.

PMID:
23777438
36.

Microcarrier culture for efficient expansion and osteogenic differentiation of human fetal mesenchymal stem cells.

Goh TK, Zhang ZY, Chen AK, Reuveny S, Choolani M, Chan JK, Oh SK.

Biores Open Access. 2013 Apr;2(2):84-97. doi: 10.1089/biores.2013.0001.

37.

Cell spreading and proliferation in response to the composition and mechanics of engineered fibrillar extracellular matrices.

Chen AK, Delrio FW, Peterson AW, Chung KH, Bhadiraju K, Plant AL.

Biotechnol Bioeng. 2013 Oct;110(10):2731-41. doi: 10.1002/bit.24921. Epub 2013 Apr 22.

PMID:
23568715
38.

Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: achievements and future direction.

Chen AK, Reuveny S, Oh SK.

Biotechnol Adv. 2013 Nov 15;31(7):1032-46. doi: 10.1016/j.biotechadv.2013.03.006. Epub 2013 Mar 24. Review.

39.

Microcarrier suspension cultures for high-density expansion and differentiation of human pluripotent stem cells to neural progenitor cells.

Bardy J, Chen AK, Lim YM, Wu S, Wei S, Weiping H, Chan K, Reuveny S, Oh SK.

Tissue Eng Part C Methods. 2013 Feb;19(2):166-80. doi: 10.1089/ten.TEC.2012.0146. Epub 2012 Sep 4.

PMID:
22834957
40.

Legislation restricting access to indoor tanning throughout the world.

Pawlak MT, Bui M, Amir M, Burkhardt DL, Chen AK, Dellavalle RP.

Arch Dermatol. 2012 Sep;148(9):1006-12. doi: 10.1001/archdermatol.2012.2080.

PMID:
22801924
41.

Translating human embryonic stem cells from 2-dimensional to 3-dimensional cultures in a defined medium on laminin- and vitronectin-coated surfaces.

Heng BC, Li J, Chen AK, Reuveny S, Cool SM, Birch WR, Oh SK.

Stem Cells Dev. 2012 Jul 1;21(10):1701-15. doi: 10.1089/scd.2011.0509. Epub 2011 Dec 23.

PMID:
22034857
42.

Critical microcarrier properties affecting the expansion of undifferentiated human embryonic stem cells.

Chen AK, Chen X, Choo AB, Reuveny S, Oh SK.

Stem Cell Res. 2011 Sep;7(2):97-111. doi: 10.1016/j.scr.2011.04.007. Epub 2011 May 11.

43.

Delivery of molecular beacons for live-cell imaging and analysis of RNA.

Chen AK, Rhee WJ, Bao G, Tsourkas A.

Methods Mol Biol. 2011;714:159-74. doi: 10.1007/978-1-61779-005-8_10.

PMID:
21431740
44.

Cadmium accumulation in and tolerance of rice (Oryza sativa L.) varieties with different rates of radial oxygen loss.

Wang MY, Chen AK, Wong MH, Qiu RL, Cheng H, Ye ZH.

Environ Pollut. 2011 Jun;159(6):1730-6. doi: 10.1016/j.envpol.2011.02.025. Epub 2011 Mar 15.

PMID:
21411196
45.

Sleep deprivation and interference by emotional distracters.

Chuah LY, Dolcos F, Chen AK, Zheng H, Parimal S, Chee MW.

Sleep. 2010 Oct;33(10):1305-13.

46.

Expansion of human embryonic stem cells on cellulose microcarriers.

Chen AK, Chen X, Choo AB, Reuveny S, Oh SK.

Curr Protoc Stem Cell Biol. 2010 Sep;Chapter 1:Unit 1C.11. doi: 10.1002/9780470151808.sc01c11s14.

PMID:
20814936
47.

An unnatural history of left ventricular pseudoaneurysm?

Chen AK, Rosenthal N, Nyberg E, Hoit BD.

J Am Soc Echocardiogr. 2010 Dec;23(12):1335.e5-7. doi: 10.1016/j.echo.2010.05.001. Epub 2010 Jun 19.

PMID:
20646911
48.

Examination of folate-targeted liposomes with encapsulated poly(2-propylacrylic acid) as a pH-responsive nanoplatform for cytosolic drug delivery.

Cheng Z, Chen AK, Lee HY, Tsourkas A.

Small. 2010 Jul 5;6(13):1398-401. doi: 10.1002/smll.201000347. No abstract available.

PMID:
20564486
49.

Ratiometric bimolecular beacons for the sensitive detection of RNA in single living cells.

Chen AK, Davydenko O, Behlke MA, Tsourkas A.

Nucleic Acids Res. 2010 Aug;38(14):e148. doi: 10.1093/nar/gkq436. Epub 2010 May 27.

50.

Immunization updates and challenges.

Keeton VF, Chen AK.

Curr Opin Pediatr. 2010 Apr;22(2):234-40. doi: 10.1097/MOP.0b013e328337685b. Review. Erratum in: Curr Opin Pediatr. 2010 Aug;22(4):559-60.

PMID:
20125025

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