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Items: 1 to 50 of 144

1.

Current outcomes when optimizing 'standard' sample preparation for single-particle cryo-EM.

Carragher B, Cheng Y, Frost A, Glaeser RM, Lander GC, Nogales E, Wang HW.

J Microsc. 2019 Sep 25. doi: 10.1111/jmi.12834. [Epub ahead of print]

PMID:
31553060
2.

Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization.

Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, Cheng C, Dingens AS, Gorman J, Sastry M, Shen CH, Zhang B, Zhou T, Chuang GY, Chao CW, Gu Y, Jafari AJ, Louder MK, O'Dell S, Rowshan AP, Viox EG, Wang Y, Choi CW, Corcoran MM, Corrigan AR, Dandey VP, Eng ET, Geng H, Foulds KE, Guo Y, Kwon YD, Lin B, Liu K, Mason RD, Nason MC, Ohr TY, Ou L, Rawi R, Sarfo EK, Schön A, Todd JP, Wang S, Wei H, Wu W; NISC Comparative Sequencing Program, Mullikin JC, Bailer RT, Doria-Rose NA, Karlsson Hedestam GB, Scorpio DG, Overbaugh J, Bloom JD, Carragher B, Potter CS, Shapiro L, Kwong PD, Mascola JR.

Cell. 2019 Jul 25;178(3):567-584.e19. doi: 10.1016/j.cell.2019.06.030.

3.

Reducing cryoEM file storage using lossy image formats.

Eng ET, Kopylov M, Negro CJ, Dallaykan S, Rice WJ, Jordan KD, Kelley K, Carragher B, Potter CS.

J Struct Biol. 2019 Jul 1;207(1):49-55. doi: 10.1016/j.jsb.2019.04.013. Epub 2019 May 21.

PMID:
31121317
4.

Visualization of clustered protocadherin neuronal self-recognition complexes.

Brasch J, Goodman KM, Noble AJ, Rapp M, Mannepalli S, Bahna F, Dandey VP, Bepler T, Berger B, Maniatis T, Potter CS, Carragher B, Honig B, Shapiro L.

Nature. 2019 May;569(7755):280-283. doi: 10.1038/s41586-019-1089-3. Epub 2019 Apr 10.

5.

Engineering the PP7 Virus Capsid as a Peptide Display Platform.

Zhao L, Kopylov M, Potter CS, Carragher B, Finn MG.

ACS Nano. 2019 Apr 23;13(4):4443-4454. doi: 10.1021/acsnano.8b09683. Epub 2019 Mar 26.

PMID:
30912918
6.

Structure-based design of a quadrivalent fusion glycoprotein vaccine for human parainfluenza virus types 1-4.

Stewart-Jones GBE, Chuang GY, Xu K, Zhou T, Acharya P, Tsybovsky Y, Ou L, Zhang B, Fernandez-Rodriguez B, Gilardi V, Silacci-Fregni C, Beltramello M, Baxa U, Druz A, Kong WP, Thomas PV, Yang Y, Foulds KE, Todd JP, Wei H, Salazar AM, Scorpio DG, Carragher B, Potter CS, Corti D, Mascola JR, Lanzavecchia A, Kwong PD.

Proc Natl Acad Sci U S A. 2018 Nov 27;115(48):12265-12270. doi: 10.1073/pnas.1811980115. Epub 2018 Nov 12.

7.

Reducing effects of particle adsorption to the air-water interface in cryo-EM.

Noble AJ, Wei H, Dandey VP, Zhang Z, Tan YZ, Potter CS, Carragher B.

Nat Methods. 2018 Oct;15(10):793-795. doi: 10.1038/s41592-018-0139-3. Epub 2018 Sep 24.

8.

Cryo-EM for Small Molecules Discovery, Design, Understanding, and Application.

Scapin G, Potter CS, Carragher B.

Cell Chem Biol. 2018 Nov 15;25(11):1318-1325. doi: 10.1016/j.chembiol.2018.07.006. Epub 2018 Aug 9. Review.

PMID:
30100349
9.

Mechanotransduction by PCDH15 Relies on a Novel cis-Dimeric Architecture.

Dionne G, Qiu X, Rapp M, Liang X, Zhao B, Peng G, Katsamba PS, Ahlsen G, Rubinstein R, Potter CS, Carragher B, Honig B, Müller U, Shapiro L.

Neuron. 2018 Aug 8;99(3):480-492.e5. doi: 10.1016/j.neuron.2018.07.006. Epub 2018 Jul 26.

10.

High resolution single particle cryo-electron microscopy using beam-image shift.

Cheng A, Eng ET, Alink L, Rice WJ, Jordan KD, Kim LY, Potter CS, Carragher B.

J Struct Biol. 2018 Nov;204(2):270-275. doi: 10.1016/j.jsb.2018.07.015. Epub 2018 Jul 25.

PMID:
30055234
11.

Routine determination of ice thickness for cryo-EM grids.

Rice WJ, Cheng A, Noble AJ, Eng ET, Kim LY, Carragher B, Potter CS.

J Struct Biol. 2018 Oct;204(1):38-44. doi: 10.1016/j.jsb.2018.06.007. Epub 2018 Jul 4.

12.

Complete functional mapping of infection- and vaccine-elicited antibodies against the fusion peptide of HIV.

Dingens AS, Acharya P, Haddox HK, Rawi R, Xu K, Chuang GY, Wei H, Zhang B, Mascola JR, Carragher B, Potter CS, Overbaugh J, Kwong PD, Bloom JD.

PLoS Pathog. 2018 Jul 5;14(7):e1007159. doi: 10.1371/journal.ppat.1007159. eCollection 2018 Jul.

13.

Benchmarking cryo-EM Single Particle Analysis Workflow.

Kim LY, Rice WJ, Eng ET, Kopylov M, Cheng A, Raczkowski AM, Jordan KD, Bobe D, Potter CS, Carragher B.

Front Mol Biosci. 2018 Jun 4;5:50. doi: 10.3389/fmolb.2018.00050. eCollection 2018.

14.

Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1.

Xu K, Acharya P, Kong R, Cheng C, Chuang GY, Liu K, Louder MK, O'Dell S, Rawi R, Sastry M, Shen CH, Zhang B, Zhou T, Asokan M, Bailer RT, Chambers M, Chen X, Choi CW, Dandey VP, Doria-Rose NA, Druz A, Eng ET, Farney SK, Foulds KE, Geng H, Georgiev IS, Gorman J, Hill KR, Jafari AJ, Kwon YD, Lai YT, Lemmin T, McKee K, Ohr TY, Ou L, Peng D, Rowshan AP, Sheng Z, Todd JP, Tsybovsky Y, Viox EG, Wang Y, Wei H, Yang Y, Zhou AF, Chen R, Yang L, Scorpio DG, McDermott AB, Shapiro L, Carragher B, Potter CS, Mascola JR, Kwong PD.

Nat Med. 2018 Jun;24(6):857-867. doi: 10.1038/s41591-018-0042-6. Epub 2018 Jun 4.

15.

Routine single particle CryoEM sample and grid characterization by tomography.

Noble AJ, Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ, Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET, Rice WJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D, des Georges A, Potter CS, Carragher B.

Elife. 2018 May 29;7. pii: e34257. doi: 10.7554/eLife.34257.

16.

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.

Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ.

Elife. 2018 Mar 29;7. pii: e33572. doi: 10.7554/eLife.33572.

17.

Structure of the insulin receptor-insulin complex by single-particle cryo-EM analysis.

Scapin G, Dandey VP, Zhang Z, Prosise W, Hruza A, Kelly T, Mayhood T, Strickland C, Potter CS, Carragher B.

Nature. 2018 Apr 5;556(7699):122-125. doi: 10.1038/nature26153. Epub 2018 Feb 28.

18.

Spotiton: New features and applications.

Dandey VP, Wei H, Zhang Z, Tan YZ, Acharya P, Eng ET, Rice WJ, Kahn PA, Potter CS, Carragher B.

J Struct Biol. 2018 May;202(2):161-169. doi: 10.1016/j.jsb.2018.01.002.

19.

Optimizing "self-wicking" nanowire grids.

Wei H, Dandey VP, Zhang Z, Raczkowski A, Rice WJ, Carragher B, Potter CS.

J Struct Biol. 2018 May;202(2):170-174. doi: 10.1016/j.jsb.2018.01.001. Epub 2018 Jan 6.

20.

Big data in cryoEM: automated collection, processing and accessibility of EM data.

Baldwin PR, Tan YZ, Eng ET, Rice WJ, Noble AJ, Negro CJ, Cianfrocco MA, Potter CS, Carragher B.

Curr Opin Microbiol. 2018 Jun;43:1-8. doi: 10.1016/j.mib.2017.10.005. Epub 2017 Oct 31. Review.

21.

Best practices for managing large CryoEM facilities.

Alewijnse B, Ashton AW, Chambers MG, Chen S, Cheng A, Ebrahim M, Eng ET, Hagen WJH, Koster AJ, López CS, Lukoyanova N, Ortega J, Renault L, Reyntjens S, Rice WJ, Scapin G, Schrijver R, Siebert A, Stagg SM, Grum-Tokars V, Wright ER, Wu S, Yu Z, Zhou ZH, Carragher B, Potter CS.

J Struct Biol. 2017 Sep;199(3):225-236. doi: 10.1016/j.jsb.2017.07.011. Epub 2017 Aug 4.

22.

Addressing preferred specimen orientation in single-particle cryo-EM through tilting.

Tan YZ, Baldwin PR, Davis JH, Williamson JR, Potter CS, Carragher B, Lyumkis D.

Nat Methods. 2017 Aug;14(8):793-796. doi: 10.1038/nmeth.4347. Epub 2017 Jul 3.

23.

Corrigendum: Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer.

Liu Q, Acharya P, Dolan MA, Zhang P, Guzzo C, Lu J, Kwon A, Gururani D, Miao H, Bylund T, Chuang GY, Druz A, Zhou T, Rice WJ, Wigge C, Carragher B, Potter CS, Kwong PD, Lusso P.

Nat Struct Mol Biol. 2017 Jun 6;24(6):553. doi: 10.1038/nsmb0617-553b. No abstract available.

PMID:
28586323
24.

Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer.

Liu Q, Acharya P, Dolan MA, Zhang P, Guzzo C, Lu J, Kwon A, Gururani D, Miao H, Bylund T, Chuang GY, Druz A, Zhou T, Rice WJ, Wigge C, Carragher B, Potter CS, Kwong PD, Lusso P.

Nat Struct Mol Biol. 2017 Apr;24(4):370-378. doi: 10.1038/nsmb.3382. Epub 2017 Feb 20. Erratum in: Nat Struct Mol Biol. 2017 Jun 6;24(6):553.

25.

Modular Assembly of the Bacterial Large Ribosomal Subunit.

Davis JH, Tan YZ, Carragher B, Potter CS, Lyumkis D, Williamson JR.

Cell. 2016 Dec 1;167(6):1610-1622.e15. doi: 10.1016/j.cell.2016.11.020.

26.

Strategies for Automated CryoEM Data Collection Using Direct Detectors.

Cheng A, Tan YZ, Dandey VP, Potter CS, Carragher B.

Methods Enzymol. 2016;579:87-102. doi: 10.1016/bs.mie.2016.04.008. Epub 2016 Jun 22. Review.

PMID:
27572724
27.

A new method for vitrifying samples for cryoEM.

Razinkov I, Dandey V, Wei H, Zhang Z, Melnekoff D, Rice WJ, Wigge C, Potter CS, Carragher B.

J Struct Biol. 2016 Aug;195(2):190-198. doi: 10.1016/j.jsb.2016.06.001. Epub 2016 Jun 8.

28.

Automated data collection in single particle electron microscopy.

Tan YZ, Cheng A, Potter CS, Carragher B.

Microscopy (Oxf). 2016 Feb;65(1):43-56. doi: 10.1093/jmicro/dfv369. Epub 2015 Dec 15. Review.

29.

Recent advances in new technologies and applications for molecular TEM.

Carragher B, Potter CS.

J Struct Biol. 2015 Nov;192(2):145. doi: 10.1016/j.jsb.2015.10.013. No abstract available.

PMID:
26534727
30.

Conformational states of the full-length glucagon receptor.

Yang L, Yang D, de Graaf C, Moeller A, West GM, Dharmarajan V, Wang C, Siu FY, Song G, Reedtz-Runge S, Pascal BD, Wu B, Potter CS, Zhou H, Griffin PR, Carragher B, Yang H, Wang MW, Stevens RC, Jiang H.

Nat Commun. 2015 Jul 31;6:7859. doi: 10.1038/ncomms8859.

31.

Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.

Mulligan SK, Speir JA, Razinkov I, Cheng A, Crum J, Jain T, Duggan E, Liu E, Nolan JP, Carragher B, Potter CS.

Microsc Microanal. 2015 Aug;21(4):1017-1025. doi: 10.1017/S1431927615014324.

32.

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.

Kang Y, Zhou XE, Gao X, He Y, Liu W, Ishchenko A, Barty A, White TA, Yefanov O, Han GW, Xu Q, de Waal PW, Ke J, Tan MH, Zhang C, Moeller A, West GM, Pascal BD, Van Eps N, Caro LN, Vishnivetskiy SA, Lee RJ, Suino-Powell KM, Gu X, Pal K, Ma J, Zhi X, Boutet S, Williams GJ, Messerschmidt M, Gati C, Zatsepin NA, Wang D, James D, Basu S, Roy-Chowdhury S, Conrad CE, Coe J, Liu H, Lisova S, Kupitz C, Grotjohann I, Fromme R, Jiang Y, Tan M, Yang H, Li J, Wang M, Zheng Z, Li D, Howe N, Zhao Y, Standfuss J, Diederichs K, Dong Y, Potter CS, Carragher B, Caffrey M, Jiang H, Chapman HN, Spence JC, Fromme P, Weierstall U, Ernst OP, Katritch V, Gurevich VV, Griffin PR, Hubbell WL, Stevens RC, Cherezov V, Melcher K, Xu HE.

Nature. 2015 Jul 30;523(7562):561-7. doi: 10.1038/nature14656. Epub 2015 Jul 22.

33.

Patterned Threadlike Micelles and DNA-Tethered Nanoparticles: A Structural Study of PEGylated Cationic Liposome-DNA Assemblies.

Majzoub RN, Ewert KK, Jacovetty EL, Carragher B, Potter CS, Li Y, Safinya CR.

Langmuir. 2015 Jun 30;31(25):7073-83. doi: 10.1021/acs.langmuir.5b00993. Epub 2015 Jun 17.

34.

CTF Challenge: Result summary.

Marabini R, Carragher B, Chen S, Chen J, Cheng A, Downing KH, Frank J, Grassucci RA, Bernard Heymann J, Jiang W, Jonic S, Liao HY, Ludtke SJ, Patwari S, Piotrowski AL, Quintana A, Sorzano CO, Stahlberg H, Vargas J, Voss NR, Chiu W, Carazo JM.

J Struct Biol. 2015 Jun;190(3):348-59. doi: 10.1016/j.jsb.2015.04.003. Epub 2015 Apr 23.

35.

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy.

Campbell MG, Veesler D, Cheng A, Potter CS, Carragher B.

Elife. 2015 Mar 11;4. doi: 10.7554/eLife.06380.

36.

Distinct conformational spectrum of homologous multidrug ABC transporters.

Moeller A, Lee SC, Tao H, Speir JA, Chang G, Urbatsch IL, Potter CS, Carragher B, Zhang Q.

Structure. 2015 Mar 3;23(3):450-460. doi: 10.1016/j.str.2014.12.013. Epub 2015 Feb 5.

37.

Structural basis for translational surveillance by the large ribosomal subunit-associated protein quality control complex.

Lyumkis D, Oliveira dos Passos D, Tahara EB, Webb K, Bennett EJ, Vinterbo S, Potter CS, Carragher B, Joazeiro CA.

Proc Natl Acad Sci U S A. 2014 Nov 11;111(45):15981-6. doi: 10.1073/pnas.1413882111. Epub 2014 Oct 27.

38.

A combined quantitative mass spectrometry and electron microscopy analysis of ribosomal 30S subunit assembly in E. coli.

Sashital DG, Greeman CA, Lyumkis D, Potter CS, Carragher B, Williamson JR.

Elife. 2014 Oct 14;3. doi: 10.7554/eLife.04491.

39.

Near-atomic resolution reconstructions using a mid-range electron microscope operated at 200 kV.

Campbell MG, Kearney BM, Cheng A, Potter CS, Johnson JE, Carragher B, Veesler D.

J Struct Biol. 2014 Nov;188(2):183-7. Epub 2014 Sep 30.

40.

Transmission electron microscopy as an orthogonal method to characterize protein aggregates.

Sung JJ, Pardeshi NN, Mulder AM, Mulligan SK, Quispe J, On K, Carragher B, Potter CS, Carpenter JF, Schneemann A.

J Pharm Sci. 2015 Feb;104(2):750-9. doi: 10.1002/jps.24157. Epub 2014 Sep 17.

41.

Molecular architecture of mammalian nitric oxide synthases.

Campbell MG, Smith BC, Potter CS, Carragher B, Marletta MA.

Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):E3614-23. doi: 10.1073/pnas.1413763111. Epub 2014 Aug 14.

42.

Uptake and transfection efficiency of PEGylated cationic liposome-DNA complexes with and without RGD-tagging.

Majzoub RN, Chan CL, Ewert KK, Silva BF, Liang KS, Jacovetty EL, Carragher B, Potter CS, Safinya CR.

Biomaterials. 2014 Jun;35(18):4996-5005. doi: 10.1016/j.biomaterials.2014.03.007. Epub 2014 Mar 21.

43.

Single-particle EM reveals the higher-order domain architecture of soluble guanylate cyclase.

Campbell MG, Underbakke ES, Potter CS, Carragher B, Marletta MA.

Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):2960-5. doi: 10.1073/pnas.1400711111. Epub 2014 Feb 10.

44.

Characterization of virus-like particles in GARDASIL® by cryo transmission electron microscopy.

Zhao Q, Potter CS, Carragher B, Lander G, Sworen J, Towne V, Abraham D, Duncan P, Washabaugh MW, Sitrin RD.

Hum Vaccin Immunother. 2014;10(3):734-9. Epub 2013 Dec 3.

45.

Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer.

Lyumkis D, Julien JP, de Val N, Cupo A, Potter CS, Klasse PJ, Burton DR, Sanders RW, Moore JP, Carragher B, Wilson IA, Ward AB.

Science. 2013 Dec 20;342(6165):1484-90. doi: 10.1126/science.1245627. Epub 2013 Oct 31.

46.

Optimod--an automated approach for constructing and optimizing initial models for single-particle electron microscopy.

Lyumkis D, Vinterbo S, Potter CS, Carragher B.

J Struct Biol. 2013 Dec;184(3):417-26. doi: 10.1016/j.jsb.2013.10.009. Epub 2013 Oct 24.

47.

Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization.

Lyumkis D, Talley H, Stewart A, Shah S, Park CK, Tama F, Potter CS, Carragher B, Horton NC.

Structure. 2013 Oct 8;21(10):1848-58. doi: 10.1016/j.str.2013.08.012. Epub 2013 Sep 19.

48.

Maximizing the potential of electron cryomicroscopy data collected using direct detectors.

Veesler D, Campbell MG, Cheng A, Fu CY, Murez Z, Johnson JE, Potter CS, Carragher B.

J Struct Biol. 2013 Nov;184(2):193-202. doi: 10.1016/j.jsb.2013.09.003. Epub 2013 Sep 12.

49.

Engineered nanostructured β-sheet peptides protect membrane proteins.

Tao H, Lee SC, Moeller A, Roy RS, Siu FY, Zimmermann J, Stevens RC, Potter CS, Carragher B, Zhang Q.

Nat Methods. 2013 Aug;10(8):759-61. doi: 10.1038/nmeth.2533. Epub 2013 Jun 30.

50.

The structure of dual-variable-domain immunoglobulin molecules alone and bound to antigen.

Correia I, Sung J, Burton R, Jakob CG, Carragher B, Ghayur T, Radziejewski C.

MAbs. 2013 May-Jun;5(3):364-72. doi: 10.4161/mabs.24258. Epub 2013 Apr 9.

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