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Items: 17

1.

A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation.

Carballeira JD, González-Pérez B, Moncalián G, de la Cruz F.

Nucleic Acids Res. 2014;42(16):10632-43. doi: 10.1093/nar/gku741. Epub 2014 Aug 14.

2.

Diplogelasinospora grovesii IMI 171018 immobilized in polyurethane foam. An efficient biocatalyst for stereoselective reduction of ketones.

Quezada MA, Carballeira JD, Sinisterra JV.

Bioresour Technol. 2012 May;112:18-27. doi: 10.1016/j.biortech.2012.02.074. Epub 2012 Mar 3.

PMID:
22424921
3.

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.

Reetz MT, Soni P, Fernández L, Gumulya Y, Carballeira JD.

Chem Commun (Camb). 2010 Dec 7;46(45):8657-8. doi: 10.1039/c0cc02657c. Epub 2010 Oct 18.

PMID:
20957255
4.

Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods.

Reetz MT, Prasad S, Carballeira JD, Gumulya Y, Bocola M.

J Am Chem Soc. 2010 Jul 7;132(26):9144-52. doi: 10.1021/ja1030479.

PMID:
20536132
5.

Microbial cells as catalysts for stereoselective red-ox reactions.

Carballeira JD, Quezada MA, Hoyos P, Simeó Y, Hernaiz MJ, Alcantara AR, Sinisterra JV.

Biotechnol Adv. 2009 Nov-Dec;27(6):686-714. doi: 10.1016/j.biotechadv.2009.05.001. Epub 2009 May 11. Review.

PMID:
19442715
6.

Monascus kaoliang CBS 302.78 immobilized in polyurethane foam using iso-propanol as co-substrate: Optimized immobilization conditions of a fungus as biocatalyst for the reduction of ketones.

Quezada MA, Carballeira JD, Sinisterra JV.

Bioresour Technol. 2009 Mar;100(6):2018-25. doi: 10.1016/j.biortech.2008.07.068. Epub 2008 Nov 28.

PMID:
19046879
7.

Changing the recognition site of a conjugative relaxase by rational design.

González-Pérez B, Carballeira JD, Moncalián G, de la Cruz F.

Biotechnol J. 2009 Apr;4(4):554-7. doi: 10.1002/biot.200800184.

PMID:
19039782
8.

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates.

Sanchis J, Fernández L, Carballeira JD, Drone J, Gumulya Y, Höbenreich H, Kahakeaw D, Kille S, Lohmer R, Peyralans JJ, Podtetenieff J, Prasad S, Soni P, Taglieber A, Wu S, Zilly FE, Reetz MT.

Appl Microbiol Biotechnol. 2008 Nov;81(2):387-97. doi: 10.1007/s00253-008-1678-9. Epub 2008 Sep 27.

PMID:
18820909
9.
10.

Alternate-site enzyme promiscuity.

Taglieber A, Höbenreich H, Carballeira JD, Mondière RJ, Reetz MT.

Angew Chem Int Ed Engl. 2007;46(45):8597-600. No abstract available.

PMID:
17912724
11.

Directed evolution and axial chirality: optimization of the enantioselectivity of Pseudomonas aeruginosa lipase towards the kinetic resolution of a racemic allene.

Carballeira JD, Krumlinde P, Bocola M, Vogel A, Reetz MT, Bäckvall JE.

Chem Commun (Camb). 2007 May 21;(19):1913-5.

PMID:
17695227
12.

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes.

Reetz MT, Carballeira JD.

Nat Protoc. 2007;2(4):891-903.

PMID:
17446890
13.

Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity.

Reetz MT, Puls M, Carballeira JD, Vogel A, Jaeger KE, Eggert T, Thiel W, Bocola M, Otte N.

Chembiochem. 2007 Jan 2;8(1):106-12.

PMID:
17133645
14.

Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability.

Reetz MT, Carballeira JD, Vogel A.

Angew Chem Int Ed Engl. 2006 Nov 27;45(46):7745-51. No abstract available.

PMID:
17075931
15.

Expanding the substrate scope of enzymes: combining mutations obtained by CASTing.

Reetz MT, Carballeira JD, Peyralans J, Höbenreich H, Maichele A, Vogel A.

Chemistry. 2006 Aug 7;12(23):6031-8.

PMID:
16789057
16.

Isolation and biochemical characterization of two novel metagenome-derived esterases.

Elend C, Schmeisser C, Leggewie C, Babiak P, Carballeira JD, Steele HL, Reymond JL, Jaeger KE, Streit WR.

Appl Environ Microbiol. 2006 May;72(5):3637-45.

17.

Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test.

Reetz MT, Bocola M, Carballeira JD, Zha D, Vogel A.

Angew Chem Int Ed Engl. 2005 Jul 4;44(27):4192-6. No abstract available.

PMID:
15929154

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